المؤلفون: Maeda, Gen¹ (AUTHOR), Baba, Misuzu² (AUTHOR), Baba, Norio^1,2 (AUTHOR) baban@cc.kogakuin.ac.jp

المصدر: Microscopy. Oct2023, Vol. 72 Issue 5, p433-445. 13p.

المؤلفون: Maeda, Gen, Baba, Misuzu, Baba, Norio

المصدر: Microscopy ; volume 68, issue Supplement_1, page i44-i44 ; ISSN 2050-5698 2050-5701

مصطلحات موضوعية: Radiology, Nuclear Medicine and imaging, Instrumentation, Structural Biology

الإتاحة: https://doi.org/10.1093/jmicro/dfz091Test
http://academic.oup.com/jmicro/article-pdf/68/Supplement_1/i44/32368406/dfz091.pdfTest

المؤلفون: KUSAKA, HIROYASU, Toyama, Yuzo, Sakai, Shinjiro, Maeda, Gen, Nishizawa, Hidemitsu, Kodama, Yu, Asahara, Shingo, Mizokami, Yuji, Horiguchi, Shin-ichiro, Nagahama, Ryuji

المصدر: Gastrointestinal Endoscopy; 2024 Supplement, Vol. 99 Issue 6, pAB603-AB604, 2p

المؤلفون: Tezuka, Shoki, Maeda, Gen, Baba, Misuzu, Baba, Norio

المصدر: Microscopy and Microanalysis ; volume 23, issue S1, page 134-135 ; ISSN 1431-9276 1435-8115

مصطلحات موضوعية: Instrumentation

الإتاحة: https://doi.org/10.1017/s1431927617001350Test
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S1431927617001350Test

المؤلفون: Maeda, Gen, Tezuka, Shoki, Sakamoto, Shohei, Baba, Misuzu, Baba, Norio

المصدر: Microscopy and Microanalysis ; volume 23, issue S1, page 138-139 ; ISSN 1431-9276 1435-8115

مصطلحات موضوعية: Instrumentation

الإتاحة: https://doi.org/10.1017/s1431927617001374Test
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S1431927617001374Test

المؤلفون: Masumoto, Tomoyuki, Maeda, Gen, Baba, Norio

مصطلحات موضوعية: Session PC6: Methods, Techniques, and Others

وصف الملف: text/html

العلاقة: http://jmicro.oxfordjournals.org/cgi/content/short/64/suppl_1/i142-aTest; http://dx.doi.org/10.1093/jmicro/dfv337Test

الإتاحة: https://doi.org/10.1093/jmicro/dfv337Test
http://jmicro.oxfordjournals.org/cgi/content/short/64/suppl_1/i142-aTest

المؤلفون: Harada, Hideaki, Miyaoka, Youichi, Yuki, Takafumi, Iwaki, Tomoyuki, Maeda, Gen, Nakahara, Ryotaro, Murakami, Daisuke, Ujihara, Tetsuro, Katsuyama, Yasushi, Hayasaka, Kenji, Kushiyama, Yoshinori, Fujishiro, Hirofumi, Amano, Yuji

المصدر: Gastrointestinal Endoscopy ; volume 93, issue 3, page 691-698 ; ISSN 0016-5107

مصطلحات موضوعية: Gastroenterology, Radiology, Nuclear Medicine and imaging

الإتاحة: https://doi.org/10.1016/j.gie.2020.09.046Test
https://api.elsevier.com/content/article/PII:S0016510720348483?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0016510720348483?httpAccept=text/plainTest

المؤلفون: Yoshiaki Kamada, Eigo Takeda, Maeda Gen, Misuzu Baba, Akira Matsuura, Sachihiko Tomonaga, Norio Baba, Masato Suzuki

المصدر: Autophagy. 15:423-437

مصطلحات موضوعية: 0301 basic medicine, Autophagosome, Electron Microscope Tomography, Saccharomyces cerevisiae Proteins, Nuclear Envelope, Research Paper - Basic Science, ATG8, Autophagy-Related Proteins, Saccharomyces cerevisiae, Vacuole, Biology, 03 medical and health sciences, Autophagy, medicine, Nuclear membrane, Molecular Biology, 030102 biochemistry & molecular biology, Endoplasmic reticulum, Autophagosomes, Membrane Proteins, Autophagy-Related Protein 8 Family, Cell Biology, Cell biology, Microscopy, Electron, 030104 developmental biology, medicine.anatomical_structure, Vacuoles, CVT complex, Biogenesis

الوصف: Macroautophagy (hereafter autophagy) is a conserved intracellular degradation mechanism required for cell survival. A double-membrane structure, the phagophore, is generated to sequester cytosolic cargos destined for degradation in the vacuole. The mechanism involved in the biogenesis of the phagophore is still an open question. We focused on 4 autophagy-related (Atg) proteins (Atg2, Atg9, Atg14, and Atg18), which are involved in the formation of the phagophore in order to gain a more complete understanding of the membrane dynamics that occur during formation of the autophagosome. The corresponding mutants, while defective in autophagy, nonetheless generate the membrane-bound form of Atg8, allowing us to use this protein as a marker for the nascent autophagosome precursor membrane. Using electron microscopy (EM), we discovered in these atg mutants a novel single-membrane structure (~120 to 150 nm in size). Electron tomography revealed that this structure originates from a part of the nuclear membrane, and we have named it the alphasome. Our data suggest that the alphasome is associated with Atg8, and sequesters selective cargo, the Cvt complex, during autophagy. Abbreviations: 3D: three-dimensional; AB: autophagic body; AP: autophagosome; Atg: autophagy-related; Cvt: cytoplasm-to-vacuole targeting; EM: electron microscopy; IEM: immunoelectron microscopy; L: lipid droplet; N: nucleus; NM: nuclear membrane; PAS: phagophore assembly site; PE: phosphatidylethanolamine; prApe1: precursor aminopeptidase I; rER: rough endoplasmic reticulum; TEM: transmission electron microscopy; V: vacuole; VLP: virus-like particle

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b0c182cee425fc2eac46886552eac11dTest
https://doi.org/10.1080/15548627.2018.1525475Test

المؤلفون: Inoue, Dai, Sekiguchi, Shuhei, Yamagata, Wataru, Maeda, Gen, Yamada, Daiki, Fujiwara, Shun, Itou, Shiori, Kurihara, Masamichi, Hijioka, Yuko, Shimoji, Kohei, Fujiki, Junko, Nakazono, Ayano, Horike, Hideyuki, Yoshioka, Atsushi, Ogura, Yuki, Hatao, Fumihiko, Imamura, Kazuhiro, Namiki, Shin

المصدر: Oncology ; volume 96, issue 3, page 140-146 ; ISSN 0030-2414 1423-0232

مصطلحات موضوعية: Cancer Research, Oncology, General Medicine

الوصف: Objectives: The neutrophil-to-lymphocyte ratio (NLR) has been proposed as an indicator of cancer-related inflammation. The aim of our study was to examine the prognostic value of the NLR for patients with advanced gastric cancer receiving second-line chemotherapy. Methods: The association of overall survival (OS) in second-line chemotherapy and the clinicopathological findings including NLR were analyzed retrospectively. The selection criteria were patients who received second-line chemotherapy between January 2010 and June 2015, had histologically confirmed gastric adenocarcinoma, and were followed up until death or for 180 days or longer. Results: Eighty-six patients met the selection criteria. Multivariate analysis revealed that performance status 2, hemoglobin < 10 g/dL, and NLR before first-line chemotherapy ≥3 were adverse predictive markers. NLR before second-line chemotherapy was not associated with OS. A prognostic model was constructed dividing patients into three groups according to the number of adverse predictive factors: good (no factor), intermediate (one factor), and poor (more than two factors). The median OS for the good, intermediate, and poor groups was 14.3, 7.2, and 4.4 months, respectively ( p < 0.001). Conclusions: Patients with advanced gastric cancer with performance status 2, hemoglobin < 10 g/dL, and NLR before first-line chemotherapy ≥3 are not likely to benefit from second-line chemotherapy.

الإتاحة: https://doi.org/10.1159/000493427Test
https://www.karger.com/Article/Pdf/493427Test

المؤلفون: Tezuka, Shoki, Maeda, Gen, Baba, Misuzu, Baba, Norio

المصدر: Microscopy ; volume 65, issue suppl 1, page i34.1-i34 ; ISSN 2050-5698 2050-5701

مصطلحات موضوعية: Radiology, Nuclear Medicine and imaging, Instrumentation, Structural Biology

الإتاحة: https://doi.org/10.1093/jmicro/dfw068Test
http://academic.oup.com/jmicro/article-pdf/65/suppl_1/i34/7365313/dfw068.pdfTest