المؤلفون: Maboudi Amir Hosein, Lotfipour Mitra Hosseini, Rasouli Milad, Azhdari Mohammad H., MacLoughlin Ronan, Bekeschus Sander, Doroudian Mohammad

المصدر: Nanotechnology Reviews, Vol 13, Iss 1, Pp 2253-69 (2024)

مصطلحات موضوعية: polymeric micelles, micelle nps, cancer treatment, drug delivery, internal and external stimuli, Technology, Chemical technology, TP1-1185, Physical and theoretical chemistry, QD450-801

الوصف: Cancer treatment often causes adverse effects and toxicity, as chemotherapy drugs affect both cancerous and healthy cells. Scientists seek to target tumor cells specifically and minimize harm to normal cells. Smart nanoparticles (NPs) are a modern technique that can release drugs when triggered by internal or external stimuli, such as temperature, pH, ultrasound, etc. This review covers stimuli-responsive micelle-based nanoparticles (SRM-NPs), a promising drug delivery platform that can enhance drug efficacy and reduce toxicity. It discusses the recent developments and applications of SRM-NPs, their responsiveness to different stimuli, and their potential to overcome drug resistance and adaptive responses. It also addresses the challenges and issues related to their stability, reproducibility, biocompatibility, safety, and optimization. The study concludes that SRM-NPs have great potential for drug delivery, but more research and development are needed to improve their clinical utility.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2191-9097Test

الوصول الحر: https://doaj.org/article/eb3650866d984b1f868da4c33a2acd2bTest

المؤلفون: Neary, Michael T., Mulder, Lianne M., Kowalski, Piotr S., MacLoughlin, Ronan, Crean, Abina M., Ryan, Katie B.

مصطلحات موضوعية: Nebulisation, RNA, Oligonucleotides, Lung, Pulmonary delivery, Nanoparticles

الوصف: In the past decade RNA-based therapies such as small interfering RNA (siRNA) and messenger RNA (mRNA) have emerged as new and ground-breaking therapeutic agents for the treatment and prevention of many conditions from viral infection to cancer. Most clinically approved RNA therapies are parenterally administered which impacts patient compliance and adds to healthcare costs. Pulmonary administration via inhalation is a non-invasive means to deliver RNA and offers an attractive alternative to injection. Nebulisation is a particularly appealing method due to the capacity to deliver large RNA doses during tidal breathing. In this review, we discuss the unique physiological barriers presented by the lung to efficient nebulised RNA delivery and approaches adopted to circumvent this problem. Additionally, the different types of nebulisers are evaluated from the perspective of their suitability for RNA delivery. Furthermore, we discuss recent preclinical studies involving nebulisation of RNA and analysis in in vitro and in vivo settings. Several studies have also demonstrated the importance of an effective delivery vector in RNA nebulisation therefore we assess the variety of lipid, polymeric and hybrid-based delivery systems utilised to date. We also consider the outlook for nebulised RNA medicinal products and the hurdles which must be overcome for successful clinical translation. In summary, nebulised RNA delivery has demonstrated promising potential for the treatment of several lung-related conditions such as asthma, COPD and cystic fibrosis, to which the mode of delivery is of crucial importance for clinical success.

وصف الملف: application/pdf

العلاقة: info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2275/IE/Synthesis and Solid State Pharmaceutical Centre (SSPC)/; info:eu-repo/grantAgreement///101041424//Synthetic Circular RNA therapeutics for prevention of sepsis-associated organ failure/CIRCLE; Journal of Controlled Release; Neary, M.T., Mulder, L.M., Kowalski, P.S., MacLoughlin, R., Crean, A.M. and Ryan, K.B. (2024) ‘Nebulised delivery of RNA formulations to the lungs: From aerosol to cytosol’, Journal of Controlled Release, 366, pp. 812–833. Available at: https://doi.org/10.1016/j.jconrel.2023.12.012Test; https://doi.org/10.1016/j.jconrel.2023.12.012Test; 833; 812; https://hdl.handle.net/10468/15476Test; 366

الإتاحة: https://doi.org/10.1016/j.jconrel.2023.12.012Test
https://hdl.handle.net/10468/15476Test

المؤلفون: Solé‐Porta, Anna, Areny‐Balagueró, Aina, Camprubí‐Rimblas, Marta, Fernández Fernández, Elena, O’Sullivan, Andrew, Giannoccari, Rossella, MacLoughlin, Ronan, Closa, Daniel, Artigas, Antonio, Roig, Anna

المساهمون: Ministerio de Ciencia e Innovación, Fundación Ramón Areces, Ministerio de Ciencia, Innovación y Universidades

المصدر: Small Science ; ISSN 2688-4046 2688-4046

الوصف: Acute respiratory distress syndrome (ARDS) is a clinical syndrome characterized by acute hypoxemic respiratory failure. Pneumonia and sepsis are the most common causes, turning ARDS into a critical public health problem. Despite recent advances in pharmacological strategies, clinical trials have not demonstrated a reduction in ARDS‐associated mortality. This is in part connected to the singularity of the pulmonary physiological barrier, which hampers drug delivery, specifically at distal areas. To this aim, the use of polymeric nanocarriers as a platform for the efficient delivery of therapeutics to the lungs by nebulization is introduced. Herein, poly(lactic‐co‐glycolic acid) (PLGA) nanocapsules (NCs) loaded with human serum albumin, as an inhalable nanotherapeutic are prepared. The production of stable NCs aerosols in the inhalable range is achieved using a commercial device, while the nanocarrier's physicochemical parameters are only minimally altered after nebulization. Importantly, in vivo studies with healthy and acute lung injury animals show that after inhalation, the NCs are homogeneously distributed throughout the lungs, arriving at the distal areas. The NCs are internalized by alveolar type II cells, avoiding macrophage‐mediated lung clearance. These features make the PLGA NCs excellent vehicles for noninvasive pulmonary delivery, facilitating a ready‐to‐be‐used nanomedicine.

الإتاحة: https://doi.org/10.1002/smsc.202400066Test

المؤلفون: Reilly, Leanne, Mac Giolla Eain, Marc, Murphy, Sarah, O’Sullivan, Andrew, Joyce, Mary, MacLoughlin, Ronan

المصدر: Frontiers in Medicine ; volume 10 ; ISSN 2296-858X

مصطلحات موضوعية: General Medicine

الوصف: Introduction Aerosol therapy is often prescribed concurrently during invasive mechanical ventilation (IMV). This study determines the effects of nebuliser position, circuit humidification source, and most importantly, lung health on the delivery of aerosol in simulated adult and paediatric IMV patients. Furthermore, the influence of closed suction catheters on aerosol delivery is also addressed. Methods A vibrating mesh nebuliser was used to deliver Albuterol to simulated adult and paediatric IMV patients with differing states of lung health. Four different nebuliser positions and two types of humidification were analysed. Closed suction catheter mounts, a mainstay in IMV therapy, were incorporated into the circuits. The mean ± SD dose of aerosol (%) was assayed from a filter at the distal end of the endotracheal tube. Results Nebuliser placement and circuit humidification source had no effect on the delivered dose (%) in adults, yet both significantly did in the simulated paediatric patients. The use of closed suction catheter mounts significantly reduced the delivered dose (%) in adults but not in paediatric patients. A simulated healthy lung state generated the largest delivered dose (%), irrespective of nebuliser position in the adult. However, different lung health and nebuliser positions yielded higher delivered doses (%) in paediatrics. Conclusion Lung health and respiratory circuit composition significantly affect aerosol delivery in both adult and paediatric IMV patients. Nebuliser placement and respiratory circuit humidification source do not affect the delivered dose in adult but do in paediatric IMV patients.

الإتاحة: https://doi.org/10.3389/fmed.2023.1307301Test

المؤلفون: Bahlool, Ahmad Z., Cavanagh, Brenton, Sullivan, Andrew O’, MacLoughlin, Ronan, Keane, Joseph, Sullivan, Mary P O’, Cryan, Sally-Ann

المساهمون: Aerosol Society, RCSI Royal College of Surgeons in Ireland Dublin, Science Foundation Ireland

المصدر: European Journal of Pharmaceutical Sciences ; volume 196, page 106734 ; ISSN 0928-0987

مصطلحات موضوعية: Pharmaceutical Science

الإتاحة: https://doi.org/10.1016/j.ejps.2024.106734Test
https://api.elsevier.com/content/article/PII:S0928098724000459?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0928098724000459?httpAccept=text/plainTest

المؤلفون: Sunkara, Krishna, Allam, Venkata Raj, Shukla, Shakti D., Chellappan, Dinesh K., Gupta, Gaurav, MacLoughlin, Ronan, Dua, Kamal

المصدر: EXCLI Journal. 20:248-251

وصف الملف: electronic

الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-438805Test
https://doi.org/10.17179/excli2021-3322Test
https://uu.diva-portal.org/smash/get/diva2:1540888/FULLTEXT01.pdfTest

المؤلفون: Fernández, Elena Fernández, MacLoughlin, Ronan

المصدر: Humidification in the Intensive Care Unit ; page 323-336 ; ISBN 9783031239526 9783031239533

الوصف: Humidification of the patient airways during respiratory support interventions is a critical aspect of care and with significant demonstrated benefit. Humidification can be broadly classified as active, through the use of heated humidifiers or passive and heat and moisture exchangers. Meta-analysis would suggest that both are comparable with respect to clinical outcomes. Hygroscopic growth of aerosol droplets is well-described and understood; however, the effect of humidification on the consequent delivered dose to the patient is not. This is important given the prevalence of aerosol therapy in the intensive care setting and the reported practices of turning off the humidifier or removing the heat and moisture exchangers during treatment. In this chapter, we review the current state-of-the-art literature across both in vitro bench assessments and in vivo human studies. The key finding from our review is that the limited number of clinical assessments of the effect of humidification on aerosol therapy found there to be no effect, with measures such as urinary drug levels showing no difference with and without humidification. Interestingly, this is counter to the majority of the in vitro reports, where lower drug delivery was noted under humidified conditions. We conclude that more work is required to understand the effect on aerosol therapy in patients and why the bench studies are not predictive of the clinical findings. Finally, we provide some suggestions as to how this may be done.

الإتاحة: https://doi.org/10.1007/978-3-031-23953-3_35Test

المؤلفون: Fernández, Elena Fernández, MacLoughlin, Ronan

المصدر: Humidification in the Intensive Care Unit ; page C1-C2 ; ISBN 9783031239526 9783031239533

الإتاحة: https://doi.org/10.1007/978-3-031-23953-3_38Test

المؤلفون: Gonzalez, Hector, McCarthy, Sean, Masterson, Claire, Byrnes, Declan, Sallent, Ignacio, Horan, Emma, Elliman, Stephen J, Vella, Gabriele, Mello, Adriele P, Silva, Johnatas D, Krasnodembskaya, Anna D, MacLoughlin, Ronan, Laffey, John G, O'Toole, Daniel

المصدر: Gonzalez , H , McCarthy , S , Masterson , C , Byrnes , D , Sallent , I , Horan , E , Elliman , S J , Vella , G , Mello , A P , Silva , J D , Krasnodembskaya , A D , MacLoughlin , R , Laffey , J G & O'Toole , D 2023 , ' Nebulised mesenchymal stem cell derived extracellular vesicles ameliorate E. coli induced pneumonia in a rodent model ' , Stem Cell Research & Therapy , vol. 14 , 151 . https://doi.org/10.1186/s13287-023-03385-6Test

الوصف: BACKGROUND: Mesenchymal stem cell (MSC) derived extracellular vesicles (EVs) have been proposed as an alternative to cell therapy, creating new possible delivery modalities such as nebulisation. We wished to investigate the therapeutic potential of directly nebulised MSC-EVs in the mitigation of Escherichia coli-induced pneumonia. METHODS: EV size, surface markers and miRNA content were assessed pre- and post-nebulisation. BEAS2B and A459 lung cells were exposed to lipopolysaccharide (LPS) and treated with nebulised bone marrow (BM) or umbilical cord (UC) MSC-EVs. Viability assays (MTT) and inflammatory cytokine assays were performed. THP-1 monocytes were stimulated with LPS and nebulised BM- or UC-EVs and phagocytosis activity was measured. For in vivo experiments, mice received LPS intratracheally (IT) followed by BM- or UC-EVs intravenously (IV) and injury markers assessed at 24 h. Rats were instilled with E. coli bacteria IT and BM- or UC-EVs delivered IV or by direct nebulisation. At 48 h, lung damage was assessed by physiological parameters, histology and inflammatory marker presence. RESULTS: MSC-EVs retained their immunomodulatory and wound healing capacity after nebulisation in vitro. EV integrity and content were also preserved. Therapy with IV or nebulised MSC-EVs reduced the severity of LPS-induced lung injury and E. coli-induced pneumonia by reducing bacterial load and oedema, increasing blood oxygenation and improving lung histological scores. MSC-EV treated animals also showed lower levels of inflammatory cytokines and inflammatory-related markers. CONCLUSIONS: MSC-EVs given IV attenuated LPS-induced lung injury, and nebulisation of MSC-EVs did not affect their capacity to attenuate lung injury caused by E. coli pneumonia, as evidenced by reduction in bacterial load and improved lung physiology.

وصف الملف: application/pdf

العلاقة: https://pure.qub.ac.uk/en/publications/8f63c12a-adb3-4ef4-96bb-ac23438a5275Test

الإتاحة: https://doi.org/10.1186/s13287-023-03385-6Test
https://pure.qub.ac.uk/en/publications/8f63c12a-adb3-4ef4-96bb-ac23438a5275Test
https://pureadmin.qub.ac.uk/ws/files/470194468/s13287_023_03385_6.pdfTest

المؤلفون: Creane, Shannice, Joyce, Mary, MacLoughlin, Ronan, Weldon, Sinéad, Dalton, John P, Taggart, Clifford C

المصدر: Creane , S , Joyce , M , MacLoughlin , R , Weldon , S , Dalton , J P & Taggart , C C 2023 , ' In vitro evaluation of the potential use of snake-derived peptides in the treatment of respiratory infections using inhalation therapy: A proof of concept study ' , European Journal of Pharmaceutical Sciences , vol. 183 , 106398 . https://doi.org/10.1016/j.ejps.2023.106398Test

مصطلحات موضوعية: Peptide, Nebulisation, Anti-bacterial, Anti-inflammatory

الوصف: Inhalation therapy using nebulisers is an attractive non-invasive route for drug delivery, particularly for the treatment of lung infections with anti-inflammatory and anti-microbial compounds. This study evaluated the suitability of three snake-derived peptides (termed Sn1b, SnE1 and SnE1-F), which we have recently shown have potent anti-inflammatory and bacteriostatic activities, for nebulisation using a vibrating mesh nebuliser (VMN). The effect of nebulisation on peptide concentration, stability and function were assessed, prior to progression to aerodynamic particle size distribution, and in vitro drug delivery in simulated adult spontaneous breathing and mechanical ventilated patient models. When nebulised, all three peptides exhibited similar functions to their non-nebulised counterparts and were found to be respirable during simulated mechanical ventilation. Based on the assessment of the droplet distributions of nebulised peptides using a Next Generation Impactor (NGI) demonstrated that if administered in vivo each peptide would likely be delivered to the lower airways. These data suggest that nebulisation using a VMN is a viable means of anti-microbial / anti-inflammatory peptide delivery targeting microbial respiratory infections, and possibly even systemic infections.

وصف الملف: application/pdf

العلاقة: https://pure.qub.ac.uk/en/publications/f3ef4f25-52b6-4e42-9660-5fe18d0d9a3dTest

الإتاحة: https://doi.org/10.1016/j.ejps.2023.106398Test
https://pure.qub.ac.uk/en/publications/f3ef4f25-52b6-4e42-9660-5fe18d0d9a3dTest
https://pureadmin.qub.ac.uk/ws/files/434165437/1_s2.0_S0928098723000295_main.pdfTest