المؤلفون: Thomas Ehammer, Kathrin Yen, Erich Sorantin, M.H. Borkenstein, Eva Scheurer, Thomas Widek, Astrid Krauskopf, Martin Urschler

المصدر: Forensic Science International. 266:281-288

مصطلحات موضوعية: medicine.medical_specialty, Radiography, Pilot Projects, 030218 nuclear medicine & medical imaging, Pathology and Forensic Medicine, 03 medical and health sciences, 0302 clinical medicine, Age Determination by Skeleton, medicine, Humans, 030216 legal & forensic medicine, Greulich pyle, Grading (tumors), medicine.diagnostic_test, business.industry, Magnetic resonance imaging, Bone age, Magnetic Resonance Imaging, Surgery, Hand Bones, Age estimation, Sample size determination, Radiological weapon, business, Nuclear medicine, Law

الوصف: Determination of skeletal development is a key pillar in forensic age estimation of living persons. Radiological assessment of hand bone age is widely used until the age of about 17-18 years, applying visual grading techniques to hand radiographs. This study investigated whether Greulich-Pyle (GP) and Tanner-Whitehouse (TW2) grading can be equally used for magnetic resonance imaging (MRI) data, which would offer the huge benefit of avoiding ionizing radiation. In 18 subjects aged between 7 and 17 years a radiograph and an MRI scan of the hand were performed. Epiphyseal ossification of hand bones was rated by two blinded radiologists with both GP and TW2. Correlation between hand MRIs and radiographs was analyzed by linear regression and inter-observer agreement was assessed. Correlation between age estimates from MRI and radiographs was high for both GP (r(2)=0.98) and TW2 (r(2)=0.93). MRI showed a tendency to estimate age slightly lower for 14-18 year-olds, which would be favorable regarding majority age determination in case this result could be reproduced using a currently not existing reference estimation method based on MRI data. Inter-observer agreement was similar for GP in radiographs and MRI, while for TW2, agreement in MRI was lower than in radiographs. In spite of limitations regarding sample size and recruited subjects, our results indicate that the use of GP and TW2 on MRI data offers the possibility of hand bone age estimation without the need for ionizing radiation.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14e93f0d45265caeed9b30f46c252888Test
https://doi.org/10.1016/j.forsciint.2016.06.016Test

المؤلفون: Wolfgang Muntean, Maximilian Leberl, Bettina Leschnik, Christina Cimenti, Elke Fröhlich-Reiterer, Gudrun Weinhandl, Axel Schlagenhauf, M.H. Borkenstein, Elisabeth Suppan, Harald Haidl, Hildegard Jasser-Nitsche

المصدر: Thrombosis research. 148

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Adolescent, medicine.medical_treatment, Antithrombin III, Type 2 diabetes, 030204 cardiovascular system & hematology, 03 medical and health sciences, Tissue factor, Young Adult, 0302 clinical medicine, Thrombin, Internal medicine, Diabetes mellitus, medicine, Humans, Hypoglycemic Agents, Insulin, 030212 general & internal medicine, Child, Blood Coagulation, Prothrombin time, Type 1 diabetes, medicine.diagnostic_test, business.industry, Antithrombin, Hematology, medicine.disease, Endocrinology, Diabetes Mellitus, Type 1, Case-Control Studies, Child, Preschool, Female, business, circulatory and respiratory physiology, medicine.drug, Peptide Hydrolases

الوصف: Background Micro- and macrovascular diseases are frequent complications in patients with diabetes. Hypercoagulability may contribute to microvascular alterations. Objective In this study, we investigated whether type 1 diabetes in children is associated with a hypercoagulable state by performing a global function test of coagulation – the thrombin generation assay. Subjects 75 patients with type 1 diabetes aged between 2 and 19 years were compared to an age-matched healthy control group. Diabetes patients were divided into high-dose and low-dose insulin cohorts with a cut-off at 0.8 U kg − 1 d − 1 . Methods Measurements were performed with platelet poor plasma using Calibrated Automated Thrombography and 1 pM or 5 pM tissue factor. Additionally, we quantified prothrombin fragments F1 + 2, thrombin-antithrombin complex, prothrombin, tissue factor pathway inhibitor, and antithrombin. Results Patients with type 1 diabetes exhibited a significantly shorter of lag time as well as decreased thrombin peak and endogenous thrombin potential compared to control subjects with 5 pM but not with 1 pM tissue factor. In high-dose insulin patients peak thrombin generation was higher and time to peak shorter than in low-dose patients. Thrombin-antithrombin complex was decreased in patients with type 1 diabetes, whereas prothrombin fragments F1 + 2 was comparable in both groups. Thrombin generation parameters did not correlate with parameters of metabolic control and the duration of diabetes. Conclusions Taken together, we found only minor changes of thrombin generation in children and adolescents with type 1 diabetes which - in contrast to type 2 diabetes - do not argue for a hypercoagulable state.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4229befe219d14ed4af328fed42604aTest
https://pubmed.ncbi.nlm.nih.gov/27776253Test

المؤلفون: Maria Fritsch, Ines Schwarz, Thomas Waldhör, M.H. Borkenstein, Birgit Rami-Merhar, Elke Fröhlich-Reiterer, Claudia Steigleder-Schweiger, Edith Schober

المصدر: European Journal of Pediatrics. 171:1193-1202

مصطلحات موضوعية: Male, Pediatrics, medicine.medical_specialty, Time Factors, Waist, Adolescent, Cross-sectional study, medicine.medical_treatment, Age Distribution, Risk Factors, Prevalence, medicine, Humans, Hypoglycemic Agents, Insulin, Obesity, Age of Onset, Sex Distribution, Young adult, Risk factor, Child, Dyslipidemias, Glycemic, Glycated Hemoglobin, Type 1 diabetes, business.industry, medicine.disease, Cross-Sectional Studies, Diabetes Mellitus, Type 1, Cardiovascular Diseases, Austria, Child, Preschool, Hypertension, Pediatrics, Perinatology and Child Health, Female, business, Body mass index, Biomarkers

الوصف: Mortality of cardiovascular diseases in patients with type 1 diabetes is increased 2- to 20-fold compared to non-diabetic individuals. In young adults with type 1 diabetes, cardiovascular events are more often the cause of premature death than nephropathy. The aim of this study was to evaluate the prevalence and extent of cardiovascular risk factors in children and adolescents with type 1 diabetes in Austria. In a cross sectional study data of children with type 1 diabetes

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b83ec1e7e9dbb0f9249fcdbe86035182Test
https://doi.org/10.1007/s00431-012-1704-xTest

المؤلفون: Bert Nagel, Andreas Gamillscheg, Heinz Zotter, M.H. Borkenstein, Reingard Aigner, Werner Sauseng

المصدر: Scandinavian Journal of Medicine & Science in Sports. 21:e100-e105

مصطلحات موضوعية: medicine.medical_specialty, School age child, business.industry, digestive, oral, and skin physiology, Significant difference, Acylated ghrelin, Physical Therapy, Sports Therapy and Rehabilitation, Peptide hormone, Endocrinology, Time exercise, Internal medicine, Medicine, Population study, Orthopedics and Sports Medicine, Ghrelin, Exercise physiology, business, hormones, hormone substitutes, and hormone antagonists

الوصف: Ghrelin is a peptide hormone, which plays an important role in appetite regulation. The effects of exercise on ghrelin plasma concentrations are still not clear, especially in children and adolescents. The aim of this study was to investigate the response of acylated and total ghrelin concentrations to controlled exercise in school-aged children. Thirty-six healthy school-aged children (mean age 12.61 years, SD ± 0.39) underwent a controlled bicycle exercise test. Before and immediately after exercise, blood samples were taken in order to measure plasma ghrelin concentrations. The control group consisted of 24 healthy school-aged children. After controlled short-time exercise, total ghrelin concentrations showed no significant difference, whereas acylated ghrelin concentrations increased significantly (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::0597ba292de3bb88206e22a297ed1afaTest
https://doi.org/10.1111/j.1600-0838.2010.01165.xTest

المؤلفون: M.H. Borkenstein, Elke Fröhlich-Reiterer

المصدر: Pädiatrie & Pädologie. 44:10-14

مصطلحات موضوعية: Gynecology, medicine.medical_specialty, business.industry, Pediatrics, Perinatology and Child Health, medicine, business

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::509d61d00afb21ea3e1131145db05a59Test
https://doi.org/10.1007/s00608-009-0154-9Test

المؤلفون: Siegfried Gallistl, Reingard Aigner, M.H. Borkenstein, Gerhard Pichler, Reinhold Kerbl, Heinz Zotter

المصدر: Metabolism. 54:862-865

مصطلحات موضوعية: Leptin, Male, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Short stature, Body Mass Index, Growth hormone deficiency, Endocrinology, Internal medicine, medicine, Humans, Insulin, Insulin-Like Growth Factor I, Child, Pancreatic hormone, business.industry, Insulin tolerance test, medicine.disease, Body Height, Idiopathic short stature, Growth Hormone, Female, medicine.symptom, business, Body mass index

الوصف: The aim of the study was to investigate the effect of standard insulin tolerance test on plasma leptin levels in children with idiopathic short stature (ISS) and in children with growth hormone deficiency (GHD). Furthermore, plasma leptin levels were analyzed with regard to age, body mass index (BMI), and plasma levels of human growth hormone and of insulin-like growth factor-1 (IGF-1). Sixty-three patients with a height below the third percentile, an age of 10.24 ± 0.40 years and a BMI standard deviation score (SDS) of −0.78 ± 0.13 (weight SDS −0.07 ± 0.12; height SDS −2.39 ± 0.10) were investigated (mean ± SD). Based on responses to insulin tolerance test, the patients were classified as ISS (n = 49) or GHD (n = 14). Plasma leptin levels were significantly lower in all patients 60 minutes (P < .001) and 120 minutes (P < .001) after insulin administration. This effect was independent of GHD, and no difference in leptin decrease was found when comparing patients with ISS to those with GHD. A correlation was found when comparing plasma leptin levels of all patients to BMI SDS (r = 0.43; P < .001) and plasma IGF-1 levels (r = 0.31; P < .01). Furthermore, positive correlation was found when BMI SDS was compared to IGF-1 (r = 0.25; P < .05). In summary, we found that insulin administration in children with short stature decreases plasma leptin levels, equally in those with and without GHD.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4c195df00c0dda5349f87945584bd34Test
https://doi.org/10.1016/j.metabol.2005.01.032Test

المؤلفون: Hildegard Jasser-Nitsche, Elisabeth Suppan, Reingard Aigner, M.H. Borkenstein, Julia Huber, Karl Sudi, Gudrun Weinhandl, Elke Fröhlich-Reiterer

المصدر: Pediatric Diabetes. 11:383-385

مصطلحات موضوعية: Male, medicine.medical_specialty, Adolescent, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Physical activity, Motor Activity, Hba1c level, Internal medicine, Diabetes mellitus, Internal Medicine, medicine, Humans, Insulin, Insulin-Like Growth Factor I, Child, Glycated Hemoglobin, Type 1 diabetes, Human Growth Hormone, business.industry, medicine.disease, Ghrelin, Insulin-Like Growth Factor Binding Protein 3, Endocrinology, Basal (medicine), Metabolic control analysis, Pediatrics, Perinatology and Child Health, Female, business, hormones, hormone substitutes, and hormone antagonists

الوصف: Huber J, Frohlich-Reiterer EE, Sudi K, Suppan E, Weinhandl G, Jasser-Nitsche H, Aigner R, Borkenstein MH. The influence of physical activity on ghrelin and IGF-1/IGFBP-3 levels in children and adolescents with type 1 diabetes mellitus. Objectives: The aim of the study was to determine the influence of regular physical activity on ghrelin and IGF-1/IGFBP-3 levels during a diabetes camp. Methods: Twenty-eight children and adolescents (14 boys; mean age 12.1 yr) with type 1 diabetes mellitus (T1DM, mean duration of diabetes 4.8 yr) attending a 2-wk diabetes camp that features increased regular physical activities have been studied. Serum levels of ghrelin (total and acylated), growth hormone (GH), insulin-like growth factor-1 (IGF-1), insulin-like growth factor-bindng protein-3 (IGFBP-3) and insulin were measured in fasting state on day 1 and day 14. Improvement of metabolic control was documented by haemoglobin A1c (HbA1c). Glucose levels and insulin doses were determined daily. Results: Mean insulin dosage decreased from 0.87 to 0.78 U/kg/d, mean HbA1c levels decreased from 8.6 to 8.3%, but the changes were not statistical. There was a significant decline in total ghrelin. IGFBP-3 and IGF-1 decreased also significantly. Total basal ghrelin was inversely related to the change in IGFBP-3. Conclusions: We hypothesize an association between ghrelin and metabolic control in T1DM. Higher ghrelin levels might be associated with poor metabolic control. The dynamic of IGFBP-3 levels appears to be under the influence of basal ghrelin concentrations in T1DM.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76bda501c6f4108870b3bb7421876788Test
https://doi.org/10.1111/j.1399-5448.2009.00604.xTest

المؤلفون: S. Gallistl, K.M. Sudi, B. Leschnik, W. Muntean, M.H. Borkenstein

المصدر: Journal of pediatric endocrinologymetabolism : JPEM. 13(9)

مصطلحات موضوعية: Hemostasis, Factor VIII, Adolescent, Endocrinology, Diabetes and Metabolism, Thyroid Gland, Fibrinogen, Coronary Disease, Factor VII, Thyroxine, Endocrinology, Child, Preschool, Pediatrics, Perinatology and Child Health, Humans, Obesity, Child, Biomarkers

الوصف: Childhood obesity may be associated with thyroid dysfunction. Both obesity and hypothyroidism are related to increased risk of coronary heart disease (CHD) in adults through high levels of serum lipids and/or hemostatic abnormalities.To investigate a possible relationship between thyroid function and hemostatic markers for CHD in obese children and adolescents.Thirty-nine obese children and adolescents were investigated for thyroid function and markers for CHD after overnight fast. Thyroid hormones were measured by radioimmunoassay. Factor VII coagulant activity (VIIc) and factor VIII coagulant activity (VIIIc) were determined using one stage clotting assays; fibrinogen was measured according to the method of Clauss; von Willebrand factor antigen (vWF-Ag), tissue type plasminogen activator antigen (tPA-Ag), and plasminogen activator inhibitor-1 antigen (PAI-1-Ag) were determined by ELISA.We found a significant inverse correlation between fT4 and factor VIIc (r = -0.33, p = 0.03) and fibrinogen (r = -0.35, p = 0.02), which remained significant after adjustment for body fat mass. Factor VIIIc (r = -0.26, p = 0.066) and vWF-Ag (r = -0.28, p = 0.053) tended to be correlated negatively to fT4. fT4 did not correlate with tPA-Ag and PAI-1-Ag. fT3 was inversely related to factor VIIc (r = -0.3, p = 0.039), which was not independent of body fat mass, and showed a less impressive negative correlation with fibrinogen (r = -0.27, p = 0.058). fT3 did not correlate with vWF-Ag, tPA-Ag, or PAI-1-Ag. There was no relationship between TSH and the determined hemostatic markers.Our study demonstrates a close relationship between thyroid function and hemostatic markers for CHD in obese children and adolescents and suggests that thyroid dysfunction is associated with an unfavorable hemostatic state even in pediatric patients.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3437e6f683e23e18aa80b2ee5b93d14eTest
https://pubmed.ncbi.nlm.nih.gov/11154157Test

المؤلفون: Heinz Zotter, G. Weinhandl, Wolfgang Muntean, Karl Sudi, M.H. Borkenstein, Siegfried Gallistl

المصدر: Blood coagulationfibrinolysis : an international journal in haemostasis and thrombosis. 11(8)

مصطلحات موضوعية: Male, medicine.medical_specialty, P-selectin, Adolescent, Childhood obesity, Fibrin Fibrinogen Degradation Products, chemistry.chemical_compound, Internal medicine, D-dimer, medicine, Humans, Platelet activation, Obesity, Risk factor, Child, Blood Coagulation, Cholesterol, business.industry, Hematology, General Medicine, medicine.disease, Endothelial stem cell, P-Selectin, Endocrinology, chemistry, Child, Preschool, Female, business

الوصف: Thirty-eight obese children and adolescents were investigated for a possible relation between cholesterol and markers of platelet activation, endothelial cell dysfunction, and activation of the coagulation system. Soluble P-selectin, von Willebrand factor antigen (vWf-Ag), D-dimer, and prothrombin fragment 1 + 2 (F1 + 2) were determined by enzyme-linked immunosorbent assays, and factor VIII coagulant activity (VIIIc) was measured by means of one-stage clotting assay. Cholesterol correlated significantly with log P-selectin (r = 0.43, P = 0.003) and log D-dimer (r = 0.33, P = 0.02). Cholesterol did not correlate with vWf-Ag, factor VIIIc, and F1 + 2. Log P-selectin correlated significantly with log D-dimer (r = 0.42, P = 0.003), which remained significant after adjustment for cholesterol (P = 0.02). Log D-dimer correlated significantly with F1 + 2 (r = 0.38, P = 0.01). Our study demonstrates that, in obese children and adolescents, cholesterol is significantly associated with P-selectin and D-dimer, and suggests an unfavorable intercorrelation between metabolic and hemostatic risk factors for coronary heart disease in childhood obesity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fdfa3189918b8f210aaf6bbd0c144bc4Test
https://pubmed.ncbi.nlm.nih.gov/11132654Test

المؤلفون: Reingard Aigner, Doris Payerl, M.H. Borkenstein, Siegfried Gallistl, Wolfgang Muntean, Karl Sudi, M. Tröbinger, G. Weinhandl

المصدر: Journal of Pediatric Endocrinology and Metabolism. 14

مصطلحات موضوعية: Blood Glucose, Male, medicine.medical_specialty, Adolescent, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Adipose tissue, Endocrinology, Insulin resistance, Weight loss, Internal medicine, Abdomen, Plasminogen Activator Inhibitor 1, Weight Loss, Fibrinolysis, Electric Impedance, Humans, Insulin, Medicine, Obesity, Child, Triglycerides, Sex Characteristics, business.industry, medicine.disease, Adipose Tissue, Tissue Plasminogen Activator, Pediatrics, Perinatology and Child Health, Body Composition, Body Constitution, Regression Analysis, Female, Insulin Resistance, medicine.symptom, business, Bioelectrical impedance analysis, Plasminogen activator, Sex characteristics

الوصف: We studied i) whether short-term weight loss alters plasminogen activator inhibitor-1 antigen (PAI-1-Ag) and tissue-type plasminogen activator antigen (tPA-Ag) in obese children, and ii) whether changes in body composition and/or abdominal adiposity are responsible for changes in PAI-1 and tPA-Ag. 20 obese boys (mean age 11.9 yr) and 40 obese girls (mean age 12 yr) were studied before and after three weeks of low-caloric diet and physical activity. Body composition was assessed by means of bioelectrical impedance, and the waist-to-hip ratio (WHR) was measured. Blood samples were determined for insulin, glucose, triglycerides, PAI-1-Ag, tPA-Ag, and the fasting insulin resistance index (FIRI) was calculated. Boys had a greater WHR, higher levels of glucose, and a slightly greater FIRI than girls. Estimates of adiposity, insulin, and triglycerides were correlated with PAI-1 and tPA-Ag. WHR was significantly correlated with fibrinolytic parameters only in girls. Insulin and tPA-Ag contributed to PAI-1 (adj. R2 = 0.36, p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38947fc184e05c76714535da1cbfdbf4Test
https://doi.org/10.1515/jpem.2001.14.1.85Test