المؤلفون: M. Zanjirband, R. Hodayi, Z. Safaeinejad, M. H. Nasr-Esfahani, R. Ghaedi-Heydari

المصدر: Scientific Reports, Vol 13, Iss 1, Pp 1-15 (2023)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract The polycystic ovarian syndrome (PCOS) is closely associated with enhanced apoptosis of granulosa cells, which have a vital role in maturation of oocytes. p53 plays a critical role in the regulation of apoptosis and cell cycle arrest, metabolism and insulin resistance. The aim of this study was to investigate the impact of p53 pathway in enhancing apoptosis and abnormal function of granulosa cells. In this study, microarray analysis and RNA sequencing were downloaded from the GEO and used as datasets. Principal Component Analysis (PCA) and online SSizer tool were applied to evaluate the experiment quality control and sample sufficiency, respectively. Bioinformatics’ analyses were performed on the selected datasets, and validated by qRT-PCR and western blot analyses. Three datasets out of five ones were chosen for re-analyzing based on the PCA outcomes. 21 deregulated genes were identified via filters including p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/888a78fcb2c74d8fb1d7c48a98efec3aTest

المؤلفون: Z. Mohammadi, M. Tavalaee, P. Gharagozloo, J. R. Drevet, M. H. Nasr-Esfahani

المصدر: Basic and Clinical Andrology, Vol 30, Iss 1, Pp 1-12 (2020)

مصطلحات موضوعية: Human spermatozoa, Sperm nuclear integrity, Sperm DNA fragmentation, Sperm DNA condensation, Acridine orange staining, High DNA stainability (HDS), Medicine (General), R5-920

الوصف: Résumé Contexte Le test SCSA® (Sperm Chromatin Structure Assay), en plus d’identifier l’indice de fragmentation de l’ADN (DFI = DNA fragmentation Index), identifie également la susceptibilté à la coloration à l’acridine orange de l’ADN (HDS: High DNA Stainability), censée refléter la compaction nucléaire des spermatozoïdes. Cependant, les données sur ce que révèle exactement ce paramètre, sa pertinence et son utilité sont contradictoires. Afin de faire la lumière sur cette situation, les spermatozoïdes d’une cohorte (N = 397) d’hommes stériles ont été soumis aux tests SCSA®, TUNEL et CMA3. Dans une sous-cohorte plus petite (N = 100), la coloration au bleu d’aniline (AB) et au bleu de toluidine (TB) a été effectuée en plus. L’objectif de cette étude était donc de répondre à la question de savoir si le HDS est. un paramètre pertinent et fiable à prendre en compte? Résultats Le HDS ne semble pas être un indicateur fiable de l’intégrité nucléaire car il montre une faible corrélation avec les tests CMA3, AB et TB. La faible corrélation du HDS avec les tests de fragmentation de l’ADN du sperme (TUNEL et SCSA®) et de condensation de l’ADN (CMA3, AB et TB) suggère que ces deux paramètres pourraient être découplés. Contrairement au DFI et au TUNEL, il n’a pas été démontré que le HDS est. corrélé avec les situations cliniques classiques de l’infertilité masculine (asthénozoospermie, tératozoospermie ou asthéno-tératozoospermie). Conclusion Le HDS présente une faible corrélation avec la plupart des tests qui se concentrent spécifiquement sur le niveau de maturité du noyau du sperme. À notre connaissance, cette étude est. la première à comparer les tests SCSA®, TUNEL, AB, TB et CMA3 sur des échantillons identiques. Elle montre la puissance, la cohérence et les limites de chaque test et le soin qui doit être apporté à leur interprétation.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12610-020-00110-8Test; https://doaj.org/toc/2051-4190Test

الوصول الحر: https://doaj.org/article/313c156a27584286a90480a560e49ce2Test

المؤلفون: F. Shahsavar, A. Rezaei, M. H. Nasr Esfahani, B. Asadifar, M. R. Nasiri, M. Jafari, P. Danesh

المصدر: Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, Vol 1, Iss 3, Pp 27-32 (2007)

مصطلحات موضوعية: Antisperm Antibody, Agglutination, Flow Cytometry, Sperm Agglutination, Medicine (General), R5-920

الوصف: Background and Objectives: The immunobead binding test (IBT) and the mixed agglutination reaction (MAR) are the most commonly used methods for detection of antisperm antibodies (ASA). The detection of ASA by flow cytometry (FCM) was first described by Haas and Cunningham. Both assays can be performed as direct or indirect methods. In this study, indirect FCM was compared with the direct MAR for detection of ASA. Methods: Semen samples were obtained from 80 men (infertile couples) in Isfahan Fertility and Infertility Center. Seminal plasma samples were incubated with ASA-negative donor sperm. Then, surface-bound antibody was detected with FITC-labeled antihuman immunoglobulin directed against IgA and IgG in the indirect FCM assay. ASAs bound to the surface of patients’ sperm were detected by direct MAR test. Results: The indirect FCM correlates with direct MAR for detection of IgA antisperm antibodies (r=0.55 and P=0.006). The indirect FCM, however, does not correlate with direct MAR for the detection of IgG antisperm antibodies (r=0.25 and P=0.25). Conclusion: Some of the ASAs in seminal fluid bind to spermatozoa. Therefore, indirect tests to detect ASAs in seminal plasma are likely to miss the presence of IgG antisperm antibodies while they effectively detect IgA antisperm antibodies. Keywords: Antisperm Antibody; Agglutination; Flow Cytometry; Sperm Agglutination

وصف الملف: electronic resource

العلاقة: http://journal.muq.ac.ir/library/upload/article/86.3.13.pdfTest; https://doaj.org/toc/1735-7799Test; https://doaj.org/toc/2008-1375Test

الوصول الحر: https://doaj.org/article/836fd1fdd3e84f56b3d0fbd2c28b22f3Test

المؤلفون: Abdelhamid M. H. Nasr, Kamal Sarabandi

المصدر: IEEE Open Journal of Antennas and Propagation, Vol 3, Pp 1313-1323 (2022)

مصطلحات موضوعية: Antenna~arrays, millimeter wave antenna, multi-beam antenna, vehicle-to-everything communication, and 5G antenna, Telecommunication, TK5101-6720

الوصف: Design procedure and experimental validation for a millimeter-wave (MMW) vehicular antenna with simultaneous multi-beam, for 5G V2X application, is presented. The design overcomes the constraint for requiring both wide angular coverage and high gain through a compact multi-array design. Each array is designed to have a fixed beam direction and sufficient beamwidth so that the overall pattern guarantees simultaneous coverage in the elevation plane. The multi-array idea is a cost-effective technique compared to employing phase shifters and beam steering of a single array and avoids latency associated with beam steering. The proposed design is composed of three compact dual polarized patch arrays. Each array covers different elevation angles, and their beam maxima are chosen to be at 10°, −20° and −40° with respect to the normal of the windshield. The antenna covers the entire 28 GHz 5G band which ranges from 26.5 GHz to 29.5 GHz. Each feeding network is designed to minimize beam scanning with frequency. Dual-polarized antenna arrays are implemented to provide polarization diversity. Antenna mounting behind the laminated windshield have been investigated and proven to have a negligible effect on the antenna matching and a minor effect on the radiation pattern.

العلاقة: https://ieeexplore.ieee.org/document/9964040Test/; https://doaj.org/toc/2637-6431Test; https://doaj.org/article/f7bce493cfe54a73986935badad92875Test

الإتاحة: https://doi.org/10.1109/OJAP.2022.3224807Test
https://doaj.org/article/f7bce493cfe54a73986935badad92875Test

المؤلفون: M. Rahmani, M. Tavalaee, M. Hosseini, A. Eskandari, E. Shaygannia, N. Sadeghi, M. N. Nazem, P. Gharagozloo, J. R. Drevet, M. H. Nasr-Esfahani

المصدر: Oxidative Medicine and Cellular Longevity, Vol 2021 (2021)

مصطلحات موضوعية: Cytology, QH573-671

الوصف: Varicocele is characterized by testicular dysfunction that originates from hyperthermia and hypoxia, leading to defects in testicular tissue and altered spermatozoa structure and function. The varicocele testis is characterized by the presence of intracellular iron deposits that contribute to the associated oxidative stress. Therefore, we tested the hypothesis that administration of an iron-chelating agent, such as deferasirox (DFX), could potentially mitigate the consequences of varicocele on testicular tissue and spermatozoa. Using a well-established rat model of varicocele (VCL), we show that treatment with DFX partially improved the structure and function of the testis and spermatozoa. In particular, sperm motility was markedly restored whereas abnormal sperm morphology was only partially improved. No significant improvement in sperm count was observed that could be associated with the proapoptotic response observed following iron chelation treatment. No reduction in oxidative damage to spermatozoa was observed since lipid peroxidation and DNA integrity were not modified. This was suggested to be a result of increased oxidative stress. Finally, we also saw no indication of attenuation of the endoplasmic reticulum/unfolded protein (ER/UPR) stress response that we recently found associated with the VCL testis in rats.

العلاقة: http://dx.doi.org/10.1155/2021/6698482Test; https://doaj.org/toc/1942-0900Test; https://doaj.org/toc/1942-0994Test; https://doaj.org/article/7abcb55f4294410db9b8af8e3c7623cdTest

الإتاحة: https://doi.org/10.1155/2021/6698482Test
https://doaj.org/article/7abcb55f4294410db9b8af8e3c7623cdTest

المؤلفون: Farnoosh Jafarpour, Faezeh Ghazvini Zadegan, Somayyeh Ostadhosseini, Mehdi Hajian, Abbas Kiani-Esfahani, M H Nasr-Esfahani

المصدر: PLoS ONE, Vol 15, Iss 6, p e0233880 (2020)

مصطلحات موضوعية: Medicine, Science

الوصف: The efficiency of somatic cell nuclear transfer (SCNT) is low due to the strong resistance of somatic donor cells to epigenetic reprogramming. Many epigenetic drugs targeting DNA methylation and histone acetylation have been used in attempts to improve the in vitro and in vivo development of SCNT embryos. H3K9me3 has been shown to be an important reprogramming barrier for generating induced pluripotent stem cells (iPSCs) and SCNT embryos in mice and humans. In this study, we examined the effects of selective siRNA and chemical inhibition of H3K9me3 in somatic donor cells on the in vitro development of bovine SCNT embryos. Chaetocin, an inhibitor of SUV39H1/H2, was supplemented during the culture of donor cells. In addition, the siRNA knockdown of SUV39H1/H2 was performed in the donor cells. The effects of chaetocin and siSUV39H1/H2 on H3K9me3 and H3K9ac were quantified using flow cytometry. Furthermore, we assessed chaetocin treatment and SUV39H1/H2 knockdown on the blastocyst formation rate. Both chaetocin and siSUV39H1/H2 significantly reduced and elevated the relative intensity level of H3K9me3 and H3K9ac in treated fibroblast cells, respectively. siSUV39H1/H2 transfection, but not chaetocin treatment, improved the in vitro development of SCNT embryos. Moreover, siSUV39H1/H2 altered the expression profile of the selected genes in the derived blastocysts, similar to those derived from in vitro fertilization (IVF). In conclusion, our results demonstrated H3K9me3 as an epigenetic barrier in the reprogramming process mediated by SCNT in bovine species, a finding which supports the role of H3K9me3 as a reprogramming barrier in mammalian species. Our findings provide a promising approach for improving the efficiency of mammalian cloning for agricultural and biomedical purposes.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/8ce9306169ba4bfb8368be780272f19cTest

المؤلفون: M. H. Nasr, M. M. Elkholy, L. M. S. El-Deen, A. S. Abouhaswa, Gamal M. Turky, A. A. EL-Hamalawy

المصدر: Journal of Materials Science: Materials in Electronics. 33:15652-15664

مصطلحات موضوعية: Electrical and Electronic Engineering, Condensed Matter Physics, Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::e85589f718ea4901da26171a33172867Test
https://doi.org/10.1007/s10854-022-08469-2Test

المؤلفون: Adib Y. Nashashibi, Kamal Sarabandi, Abdelhamid M. H. Nasr

المصدر: IEEE Transactions on Instrumentation and Measurement. 70:1-11

مصطلحات موضوعية: Materials science, business.industry, Coplanar waveguide, Attenuation, Physics::Optics, Dielectric, Characterization (materials science), Planar, Reflection (mathematics), Transmission line, Attenuation coefficient, Optoelectronics, Electrical and Electronic Engineering, business, Instrumentation

الوصف: A broadband (1–35 GHz) time-/frequency-domain reflection technique for measuring the complex dielectric constant of low-loss dielectric slabs is presented. The technique uses a coplanar waveguide (CPW) transmission line sample holder for measurements. In addition to the standard substrate materials, the technique is suitable for the characterization of materials, such as glass, as it does not require complicated sample machining and preparation. The procedure is based on comparing the measured effective dielectric constant and the attenuation constants for both the loaded and unloaded CPW line sample holder. The effective dielectric constant and the attenuation constant are first modeled as a function of the real and imaginary parts of the superstrate’s dielectric constant using a 2-D numerical line calculator. Samples under test are assumed to be nonmagnetic and have their material resonances at frequencies substantially above 35 GHz. The technique is validated through measurements of known Rogers RT/duroid samples. Measured complex dielectric constants of different materials are in very good agreement with data reported in materials’ datasheets.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::21974e0f6c8bb4fd6444e8115051459aTest
https://doi.org/10.1109/tim.2021.3102742Test

المؤلفون: Esfahani, M. H. Nasr, Haghani, F. Khaksar, Heidarian, Shahram

مصطلحات موضوعية: QA Mathematics / matematika

وصف الملف: text

العلاقة: http://real.mtak.hu/153560/1/3216.pdfTest; Esfahani, M. H. Nasr and Haghani, F. Khaksar and Heidarian, Shahram (2022) Some results on I-Balls, radicals, sequences and topology in BL-Algebras. Miskolc Mathematical Notes, 23 (2). pp. 637-650. ISSN 1787-2413

الإتاحة: https://doi.org/10.18514/MMN.2022.3216Test
http://real.mtak.hu/153560Test/
http://real.mtak.hu/153560/1/3216.pdfTest

المؤلفون: M. H. Badr, Gamal Turky, M. Moustafa, A. S. Abouhaswa, M. H. Nasr, A. A. El-Hamalawy, L. M. S. El-Deen, M. M. Elkholy

المصدر: Journal of Inorganic and Organometallic Polymers and Materials

مصطلحات موضوعية: Permittivity, SCANNING ELECTRON MICROSCOPY, Materials science, Polymers and Plastics, FERRITES, Analytical chemistry, Young's modulus, SYNTHESIS (CHEMICAL), AC CONDUCTIVITY, Dielectric, DIELECTRIC LOSSES, PERMITTIVITY, engineering.material, MANGANESE, symbols.namesake, ELASTIC PROPERTIES, A.C CONDUCTIVITY, Lattice constant, DIELECTRICS PROPERTY, Materials Chemistry, NANOPARTICLES, SPECTROSCOPY [INFRARED], DIELECTRIC PROPERTIES, FERRITE, Elastic modulus, AC-CONDUCTIVITY, MANGANESE FERRITES, Bulk modulus, X-RAY DIFFRACTION, FOURIER TRANSFORM INFRARED SPECTROSCOPY, BINARY ALLOYS, MANGANESE ALLOYS, Spinel, X RAY POWDER DIFFRACTION, A.C. CONDUCTIVITY, MAGNETIC PROPERTIES, SATURATION MAGNETIZATION, X- RAY DIFFRACTIONS, SPECTRUM ANALYSIS, NANOMAGNETICS, engineering, symbols, MN CONTENT, Dielectric loss, AUTO COMBUSTION, LATTICE CONSTANTS, INFRARED SPECTROSCOPY

الوصف: Fe2Cd1-xMnxO4 (x= 0, 0.25, 0.5, 0.75, 1) spinel ferrite nanoparticle samples were synthesized using a flash auto-combustion technique and analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR). The inspection of dielectric and magnetic properties of the prepared ferrites was carried out by using broadband dielectric spectroscopy (BDS) and vibration sample magnetometer (VSM) measurements, respectively. X-ray diffraction analysis verified the formation of the main cubic phase with space group Fd3m and a decrease in lattice constants with the increase in Mn-content. FTIR study revealed the two characteristic absorption bands of spinel ferrites and their dependence on Mn-content was investigated and explained. Elastic moduli such as Longitudinal, transverse and mean elastic wave velocities (𝑉𝑙 , 𝑉𝑡 𝑉𝑚), bulk modulus (𝐵), rigidity modulus (𝐺), Young modulus (𝐸) and calculated Debye temperature (θD) have been studied and found to decrease with the increase in Mn-content. SEM analysis revealed nanoparticles agglomeration of uniform grains with increasing in the average grain size as Mn-content increased. The VSM measurements showed an increase in saturation magnetization (Ms) accompanied by a decrease in coercivity (Hc) as Mn-content increased. Dielectric investigations showed very high values of permittivity and dielectric loss at lower frequencies reflecting the combination of exchange of electrons between ferrous and ferric ions and ions’ transport. The ac-conductivity showed a plateau that yields the dc-conductivity (σdc) at lower frequencies followed by a characteristic frequency (νc) at which it tends out to follow a power law. The relation between these two parameters confirms the empirical BNN-relation.

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::becb25063058b6f5b50afd4c56675680Test
https://hdl.handle.net/10995/118382Test