المؤلفون: Chai, Min, Fu, Yueyao, Zheng, Shuihua, Hong, Zhiwei, Shao, Changxiao, Luo, Kun, Fan, Jianren

المساهمون: National Natural Science Foundation of China, Zhejiang University State Key Laboratory of Clean Energy Utilization

المصدر: International Journal of Multiphase Flow ; volume 161, page 104390 ; ISSN 0301-9322

مصطلحات موضوعية: Fluid Flow and Transfer Processes, General Physics and Astronomy, Mechanical Engineering

الإتاحة: https://doi.org/10.1016/j.ijmultiphaseflow.2023.104390Test
https://api.elsevier.com/content/article/PII:S0301932223000137?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0301932223000137?httpAccept=text/plainTest

المؤلفون: Huang, Xiaona, Luo, Kun, Shen, Yidi, Yue, Yanan, An, Qi

المصدر: Energy and AI ; volume 11, page 100210 ; ISSN 2666-5468

مصطلحات موضوعية: Artificial Intelligence, General Energy, Engineering (miscellaneous)

الإتاحة: https://doi.org/10.1016/j.egyai.2022.100210Test
https://api.elsevier.com/content/article/PII:S2666546822000568?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666546822000568?httpAccept=text/plainTest

المؤلفون: Liu, Shuang, Wang, Wei, Jia, Tianchang, Xin, Lusheng, Xu, Ting-ting, Wang, Chong, Xie, Guosi, Luo, Kun, Li, Jun, Kong, Jie, Zhang, Qingli

المساهمون: Rather, Philip N., MOA | CAFS | Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences, China Agricultural Research System

المصدر: Microbiology Spectrum ; volume 11, issue 6 ; ISSN 2165-0497

الوصف: Translucent post-larvae disease (TPD), caused by Vibrio parahaemolyticus ( Vp TPD ), has become an emerging shrimp disease, affecting more than 70%–80% of coastal shrimp nurseries in China in spring 2020. Here, we investigated the key virulence factors of Vp TPD by analyzing protein fragments, related genomic information, as well as experimental challenge tests. After investigating the toxic effects of purified protein fragments with different molecular weights (MWs) from Vp TPD, we found that only the protein fragments with MW >100 kDa showed similar lethality to live Vp TPD in the experimental challenge test using post-larvae shrimp. Meanwhile, similar histopathological changes exhibiting in the hepatopancreas and midgut of the diseased individuals were observed in the post-larvae shrimp challenged with either bacterial protein fragments (MW >100 kDa) or live Vp TPD . Based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry analyses, two novel proteins, Vibrio high virulent protein (VHVP)-1 and VHVP-2, were identified as the candidates of key virulence factors to cause TPD. Moreover, VHVP-1 and VHVP-2 were found to be encoded by two genes ( vhvp-1 and vhvp-2 ) tandemly located on a 187,791-bp plasmid and were predicted to depend on the same promoter following a comparative genomic analysis. Further epidemiological investigation and challenge test indicated that the V. parahaemolyticus isolate carrying only the vhvp-1 gene and lacking vhvp-2 gene could not cause mortality of experimental Penaeus vannamei post-larvae. The mutant (Δvhvp-2) by deleting vhvp-2 gene could only cause 4.92% of accumulative mortality of post-larvae that is similar to the non- Vp TPD Vibrio strain. Additionally, the complemented strains, Δvhvp-2/pBT3-vhvp-2 and Δvhvp-2/pwtCas9-vhvp-2, showed similar virulence to the wild-type Vp TPD . The results demonstrated that V. parahaemolyticus becomes lethal to post-larval shrimp by acquiring the VHVP-2 virulence factor. This study sheds ...

الإتاحة: https://doi.org/10.1128/spectrum.00492-23Test

المؤلفون: Luo, Kun, Li, Ya, Liu, Tong, Zhuge, Xiangqun, Chung, Etelka, Timms, Andrew R., Graham, Simon P., Ren, Guogang

المساهمون: Royal Society, National Natural Science Foundation of China, Biotechnology and Biological Sciences Research Council

المصدر: ACS Omega ; volume 8, issue 39, page 36171-36178 ; ISSN 2470-1343 2470-1343

مصطلحات موضوعية: General Chemical Engineering, General Chemistry

الإتاحة: https://doi.org/10.1021/acsomega.3c03665Test

المؤلفون: Luo, Kun, Zhou, Tao, Chen, Wei

المصدر: Phys. Rev. B 96, 245414 (2017)

مصطلحات موضوعية: Condensed Matter - Mesoscale and Nanoscale Physics, Condensed Matter - Superconductivity

الوصف: Ballistic point contact (BPC) with zigzag edges in graphene is a main candidate of a valley filter, in which the polarization of the valley degree of freedom can be selected by using a local gate voltage. Here, we propose to detect the valley filtering effect by Andreev reflection. Because electrons in the lowest conduction band and the highest valence band of the BPC possess opposite chirality, the inter-band Andreev reflection is strongly suppressed, after multiple scattering and interference. We draw this conclusion by both the scattering matrix analysis and the numerical simulation. The Andreev reflection as a function of the incident energy of electrons and the local gate voltage at the BPC is obtained, by which the parameter region for a perfect valley filter and the direction of valley polarization can be determined. The Andreev reflection exhibits an oscillatory decay with the length of the BPC, indicating a negative correlation to valley polarization.
Comment: 7 pages, 4 figures, the title is changed, and the references are updated

الوصول الحر: http://arxiv.org/abs/1707.00612Test

المؤلفون: Zhang, Shuangshuang, Li, Zihe, Luo, Kun, He, Julong, Gao, Yufei, Soldatov, Alexander V, Benavides, Vicente, Shi, Kaiyuan, Nie, Anmin, Zhang, Bin, Hu, Wentao, Ma, Mengdong, Liu, Yong, Wen, Bin, Gao, Guoying, Liu, Bing, Zhang, Yang, Shu, Yu, Yu, Dongli, Zhou, Xiang-Feng, Zhao, Zhisheng, Xu, Bo, Su, Lei, Yang, Guoqiang, Chernogorova, Olga P, Tian, Yongjun

المصدر: National Science Review. 9(1)

مصطلحات موضوعية: amorphous carbon, ultrahard, ultrastrong, semiconductor, phase transition, Experimentell fysik, Experimental Physics

الوصف: Carbon is one of the most fascinating elements due to its structurally diverse allotropic forms stemming from its bonding varieties (sp, sp2, and sp3). Exploring new forms of carbon has always been the eternal theme of scientific research. Herein, we report the amorphous (AM) carbon materials with high fraction of sp3 bonding recovered from compression of fullerene C60 under high pressure and high temperature previously unexplored. Analysis of photoluminescence and absorption spectra demonstrates that they are semiconducting with a bandgap range of 1.5–2.2 eV, comparable to that of widely used amorphous silicon. Comprehensive mechanical tests demonstrate that the synthesized AM-III carbon is the hardest and strongest amorphous material known so far, which can scratch diamond crystal and approach its strength. The produced AM carbon materials combine outstanding mechanical and electronic properties, and may potentially be used in photovoltaic applications that require ultrahigh strength and wear resistance.

وصف الملف: print

الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-88295Test
https://doi.org/10.1093/nsr/nwab140Test

المؤلفون: Hwang, Jiunn-Jer, Chen, Pei-Yu, Luo, Kun-Hao, Wang, Yung-Chin, Lai, Ting-Ying, Balitaan, Jolleen Natalie I., Lin, Shu-Rung, Yeh, Jui-Ming

المصدر: Polymers (20734360); Jun2024, Vol. 16 Issue 12, p1673, 21p

مستخلص: In this study, a series of amine-modified mesoporous silica (AMS)-based epoxy composites with superhydrophobic biomimetic structure surface of Xanthosoma sagittifolium leaves (XSLs) were prepared and applied as anti-corrosion and anti-biofilm coatings. Initially, the AMS was synthesized by the base-catalyzed sol–gel reaction of tetraethoxysilane (TEOS) and triethoxysilane (APTES) through a non-surfactant templating route. Subsequently, a series of AMS-based epoxy composites were prepared by performing the ring-opening polymerization of DGEBA with T-403 in the presence of AMS spheres, followed by characterization through FTIR, TEM, and CA. Furthermore, a nano-casting technique with polydimethylsiloxane (PDMS) as the soft template was utilized to transfer the surface pattern of natural XSLs to AMS-based epoxy composites, leading to the formation of AMS-based epoxy composites with biomimetic structure. From a hydrophilic CA of 69°, the surface of non-biomimetic epoxy significantly increased to 152° upon introducing XSL surface structure to the AMS-based epoxy composites. Based on the standard electrochemical anti-corrosion and anti-biofilm measurements, the superhydrophobic BEAMS3 composite was found to exhibit a remarkable anti-corrosion efficiency of ~99% and antimicrobial efficacy of 82% as compared to that of hydrophilic epoxy coatings. [ABSTRACT FROM AUTHOR]

: Copyright of Polymers (20734360) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Sui, Juan, Sun, Kun, Kong, Jie, Tan, Jian, Dai, Ping, Cao, Jiawang, Luo, Kun, Luan, Sheng, Xing, Qun, Meng, Xianhong

المصدر: Animals (2076-2615); Jun2024, Vol. 14 Issue 12, p1817, 15p

مستخلص: Simple Summary: This study aimed to estimate genetic parameters of growth traits and resistance to white spot syndrome virus in Pacific white shrimp (Litopenaeus vannamei). Through controlled virus challenge assays, phenotypic values for five traits were assessed: body weight, overall length, body length, tail length, and post-infection survival time. Three models were utilized, namely pedigree-based best linear unbiased prediction, genomic best linear unbiased prediction, and single-step genomic best linear unbiased prediction. Under the genomic prediction model, the heritability of growth-related traits decreased, whereas the heritability of post-infection survival time increased. Both genomic models significantly enhanced prediction accuracy compared to the pedigree-based model, with the most notable improvement observed in virus resistance. The genetic correlations between growth and WSSV resistance obtained from the three methods were consistently low and negative. These findings provide valuable insights for breeding programs and variety development in L. vannamei. The current study aimed to provide a precise assessment of the genetic parameters associated with growth and white spot syndrome virus (WSSV) resistance traits in Pacific white shrimp (Litopenaeus vannamei). This was achieved through a controlled WSSV challenge assay and the analysis of phenotypic values of five traits: body weight (BW), overall length (OL), body length (BL), tail length (TL), and survival hour post-infection (HPI). The analysis included test data from a total of 1017 individuals belonging to 20 families, of which 293 individuals underwent whole-genome resequencing, resulting in 18,137,179 high-quality SNP loci being obtained. Three methods, including pedigree-based best linear unbiased prediction (pBLUP), genomic best linear unbiased prediction (GBLUP), and single-step genomic BLUP (ssGBLUP) were utilized. Compared to the pBLUP model, the heritability of growth-related traits obtained from GBLUP and ssGBLUP was lower, whereas the heritability of WSSV resistance was higher. Both the GBLUP and ssGBLUP models significantly enhanced prediction accuracy. Specifically, the GBLUP model improved the prediction accuracy of BW, OL, BL, TL, and HPI by 4.77%, 21.93%, 19.73%, 19.34%, and 63.44%, respectively. Similarly, the ssGBLUP model improved prediction accuracy by 10.07%, 25.44%, 25.72%, 19.34%, and 122.58%, respectively. The WSSV resistance trait demonstrated the most substantial enhancement using both genomic prediction models, followed by body size traits (e.g., OL, BL, and TL), with BW showing the least improvement. Furthermore, the choice of models minimally impacted the assessment of genetic and phenotypic correlations. Genetic correlations among growth traits ranged from 0.767 to 0.999 across models, indicating high levels of positive correlations. Genetic correlations between growth and WSSV resistance traits ranged from (−0.198) to (−0.019), indicating low levels of negative correlations. This study assured significant advantages of the GBLUP and ssGBLUP models over the pBLUP model in the genetic parameter estimation of growth and WSSV resistance in L. vannamei, providing a foundation for further breeding programs. [ABSTRACT FROM AUTHOR]

: Copyright of Animals (2076-2615) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Wu, Zhengmin, Zhong, Hua, Wang, Xiaoran, Sun, Changying, Wang, Yujie, Luo, Kun, Qin, Kuan

المصدر: Journal of Food Science (John Wiley & Sons, Inc.); Jun2024, Vol. 89 Issue 6, p3629-3648, 20p

مصطلحات موضوعية: TEA, RESPONSE surfaces (Statistics), CONTINUOUS processing

مستخلص: In response to the challenges of low automation and a lack of a continuous processing system for Taiping Houkui tea, this study proposed a design scheme for a continuous processing line and built a continuous processing prototype for testing by combining the production requirements of Taiping Houkui tea, the characteristics of withered leaves, and the existing relevant production equipment. First, the physical properties of Taiping Houkui tea were determined. A simulation was performed using the Hertz–Mindlin model, and the motion states of the tea leaves were obtained under different conditions to define the parameter design range of the experimental platform and verify its structural rationality. Then, the response surface methodology was used to optimize the working parameter ranges and obtain the best working parameters for the feeding and kneading mechanisms. Finally, a continuous production prototype was constructed for further production verification. The experimental results show that the success rate of continuous production on this platform was 70.68%, with an average output of approximately 0.4 kg/h for Taiping Houkui dry tea on a single slide track, and the produced tea was similar to manually made tea. This demonstrates that the continuous production technique has high feasibility and provides a reference for continuous production of Taiping Houkui tea. [ABSTRACT FROM AUTHOR]

: Copyright of Journal of Food Science (John Wiley & Sons, Inc.) is the property of John Wiley & Sons, Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Jiang, Yang, Li, Wenhui, Luo, Kun

المصدر: ACS Sustainable Chemistry & Engineering; 5/27/2024, Vol. 12 Issue 21, p8051-8060, 10p