المؤلفون: Teresa Padilla‐Flores, Alicia Sampieri, Luis Vaca

المصدر: Pharmacology Research & Perspectives, Vol 12, Iss 3, Pp n/a-n/a (2024)

مصطلحات موضوعية: adverse effects, COVID‐19, SARS‐CoV‐2, vaccines, Therapeutics. Pharmacology, RM1-950

الوصف: Abstract Coronavirus disease 2019 (COVID‐19) caused by the severe acute respiratory syndrome coronavirus 2n first appeared in Wuhan, China in 2019. Soon after, it was declared a pandemic by the World Health Organization. The health crisis imposed by a new virus and its rapid spread worldwide prompted the fast development of vaccines. For the first time in human history, two vaccines based on recombinant genetic material technology were approved for human use. These mRNA vaccines were applied in massive immunization programs around the world, followed by other vaccines based on more traditional approaches. Even though all vaccines were tested in clinical trials prior to their general administration, serious adverse events, usually of very low incidence, were mostly identified after application of millions of doses. Establishing a direct correlation (the cause‐effect paradigm) between vaccination and the appearance of adverse effects has proven challenging. This review focuses on the main adverse effects observed after vaccination, including anaphylaxis, myocarditis, vaccine‐induced thrombotic thrombocytopenia, Guillain–Barré syndrome, and transverse myelitis reported in the context of COVID‐19 vaccination. We highlight the symptoms, laboratory tests required for an adequate diagnosis, and briefly outline the recommended treatments for these adverse effects. The aim of this work is to increase awareness among healthcare personnel about the serious adverse events that may arise post‐vaccination. Regardless of the ongoing discussion about the safety of COVID‐19 vaccination, these adverse effects must be identified promptly and treated effectively to reduce the risk of complications.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2052-1707Test

الوصول الحر: https://doaj.org/article/cc2bee9dc6574fcdb2cd9894b78f894bTest

المؤلفون: Boris Zurita-Cueva, Luis Vaca Burbano, Youmana Martillo, Cleto Ramírez, Norman López

المصدر: Interdisciplinary Neurosurgery, Vol 35, Iss , Pp 101915- (2024)

مصطلحات موضوعية: Cavernoma, Brain stem surgery, Hypoglossal trigone, Vagal trigone, Case Report, Surgery, RD1-811, Neurology. Diseases of the nervous system, RC346-429

الوصف: Background and importance: The surgical approach to the medulla oblongata poses a significant challenge for neurosurgery, considering the clinical consequences and risks of this procedure. The authors describe a step-by-step technique to safely extirpate a cavernous malformation within the hypoglossal trigone with minimal risk of neurological deficits. Clinical Presentation: In a young 33-year-old patient was identified a lesion in the center of the medulla oblongata. Complete resection of the cavernous malformation through the hypoglossal trigone was performed with an improvement of all symptoms. The key surgical tip is to dissect the lesion between the posterior median sulcus and the sulcus limitans avoiding the vagal trigone. Conclusion: Surgical approach through the hypoglossal trigone appears to be safe and effective for the treatment of medulla oblongata cavernomas. The entry zone through the dorsomedial sulcus of the hypoglossal trigone with a meticulous delicate microsurgical technique is the key to success with these kinds of lesions.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2214751923001986Test; https://doaj.org/toc/2214-7519Test

الوصول الحر: https://doaj.org/article/626930e497de4d8686b676e081aff1c9Test

المؤلفون: Alicia Sampieri, Alexander Asanov, Kevin Manuel Méndez-Acevedo, Luis Vaca

المصدر: Cells, Vol 12, Iss 19, p 2353 (2023)

مصطلحات موضوعية: SIDT2, ApoA1, cholesterol, CRAC motif, Cytology, QH573-671

الوصف: SIDT2 is a lysosomal protein involved in the degradation of nucleic acids and the transport of cholesterol between membranes. Previous studies identified two “cholesterol recognition/interaction amino acid consensus” (CRAC) motifs in SIDT1 and SIDT2 members. We have previously shown that the first CRAC motif (CRAC-1) is essential for protein translocation to the PM upon cholesterol depletion in the cell. In the present study, we show that SIDT2 and the apolipoprotein A1 (ApoA1) form a complex which requires the second CRAC-2 motif in SIDT2 to be established. The overexpression of SIDT2 and ApoA1 results in enhanced ApoA1 secretion by HepG2 cells. This is not observed when overexpressing the SIDT2 with the CRAC-2 domain mutated to render it unfunctional. All these results provide evidence of a novel role for SIDT2 as a protein forming a complex with ApoA1 and enhancing its secretion to the extracellular space.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2073-4409/12/19/2353Test; https://doaj.org/toc/2073-4409Test

الوصول الحر: https://doaj.org/article/b58ae5bff2be4eea85f60efa81ab8f02Test

المؤلفون: Adolfo Cruz-Resendiz, Gonzalo Acero, Alicia Sampieri, Goar Gevorkian, Carolina Salvador, Laura Escobar, Margarita Jacaranda Rosendo-Pineda, Mara Medeiros, Luis Vaca

المصدر: Frontiers in Immunology, Vol 13 (2022)

مصطلحات موضوعية: polyhedrin, baculovirus, occlusion bodies, nasal immunization, nanoparticles, Immunologic diseases. Allergy, RC581-607

الوصف: Polyhedrins are viral proteins present in a large family of baculoviruses that form occlusion bodies (polyhedra). These structures protect the virus particles from the outside environment until they are ingested by susceptible insects. Occluded viruses can sustain inclement weather for long periods of time. Therefore, the polyhedra is a natural preservative that keeps the viral structure intact at ambient temperature for years. In a previous study we identified the first 110 amino acids from polyhedrin (PH(1-110)) as a good candidate to carry antigens of interest. As a proof of concept, we produced a fusion protein with PH(1-110) and the green fluorescent protein (PH(1-110)GFP). The fusion protein associates spontaneously during its synthesis resulting in the formation of nanoparticles. Nasal immunization with these nanoparticles and in the absence of any adjuvant, results in a robust immune response with the production of IgG immunoglobulins that remained elevated for months and that selectively recognize the GFP but not PH(1-110). These results indicate that PH(1-110) is poorly immunogenic but capable of enhancing the immune response to GFP.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fimmu.2022.1057499/fullTest; https://doaj.org/toc/1664-3224Test

الوصول الحر: https://doaj.org/article/a4984553d3154b228e7a3a9c4b28731bTest

المؤلفون: Alicia Sampieri, Ricardo Monroy-Contreras, Alexander Asanov, Luis Vaca

المصدر: Frontiers in Bioengineering and Biotechnology, Vol 10 (2022)

مصطلحات موضوعية: molecular beacons, microarrays, silk, point-of-care diagnostics, influenza virus, Biotechnology, TP248.13-248.65

الوصف: We have developed a novel microarray system based on three technologies: 1) molecular beacons designed to interact with DNA targets at room temperature (25–27°C), 2) tridimensional silk-based microarrays containing the molecular beacons immersed in the silk hydrogel, and 3) shallow angle illumination, which uses separated optical pathways for excitation and emission. Unlike conventional microarrays that exhibit reduced signal-to-background ratio, require several stages of incubation, rinsing, and stringency control, and measure only end-point results, our microarray technology provides enhanced signal-to-background ratio (achieved by separating the optical pathways for excitation and emission, resulting in reduced stray light), performs analysis rapidly in one step without the need for labeling DNA targets, and measures the entire course of association kinetics between target DNA and the molecular beacons. To illustrate the benefits of our technology, we conducted microarray assays designed for the identification of influenza viruses. We show that in a single microarray slide, we can identify the virus subtype according to the molecular beacons designed for hemagglutinin (H1, H2, and H3) and neuraminidase (N1, N2). We also show the identification of human and swine influenza using sequence-specific molecular beacons. This microarray technology can be easily implemented for reagentless point-of-care diagnostics of several contagious diseases, including coronavirus variants responsible for the current pandemic.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fbioe.2022.881679/fullTest; https://doaj.org/toc/2296-4185Test

الوصول الحر: https://doaj.org/article/9fa2cbf8cbfc4373bd55d0a772a51147Test

المؤلفون: Aaron Pavel Rodríguez-Hernández, Daniel Martínez-Flores, Adolfo Cruz-Reséndiz, Teresa Padilla-Flores, Rodrigo González-Flores, Kenia Estrada, Alicia Sampieri, Aldo Román Camacho-Zarco, Luis Vaca

المصدر: Viruses, Vol 15, Iss 2, p 411 (2023)

مصطلحات موضوعية: baculovirus display, GP64, VP39, vaccines, Microbiology, QR1-502

الوصف: Baculoviridae is a large family of arthropod-infective viruses. Recombinant baculoviruses have many applications, the best known is as a system for large scale protein production in combination with insect cell cultures. More recently recombinant baculoviruses have been utilized for the display of proteins of interest with applications in medicine. In the present review we analyze the different strategies for the display of proteins and peptides on the surface of recombinant baculoviruses and provide some examples of the different proteins displayed. We analyze briefly the commercially available systems for recombinant baculovirus production and display and discuss the future of this emerging and powerful technology.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1999-4915/15/2/411Test; https://doaj.org/toc/1999-4915Test

الوصول الحر: https://doaj.org/article/914f0e5d6ce54c049b6f25532a321983Test

المؤلفون: Adolfo Cruz-Reséndiz, Jesús Zepeda-Cervantes, Alicia Sampieri, Carlos Bastián-Eugenio, Gonzalo Acero, J. Iván Sánchez-Betancourt, Goar Gevorkian, Luis Vaca

المصدر: BMC Biotechnology, Vol 20, Iss 1, Pp 1-14 (2020)

مصطلحات موضوعية: Vaccines, Particles, Self-assembling, Thermostable, Immunology, Biotechnology, TP248.13-248.65

الوصف: Abstract Background The use of biomaterials has been expanded to improve the characteristics of vaccines. Recently we have identified that the peptide PH (1–110) from polyhedrin self-aggregates and incorporates foreign proteins to form particles. We have proposed that this peptide can be used as an antigen carrying system for vaccines. However, the immune response generated by the antigen fused to the peptide has not been fully characterized. In addition, the adjuvant effect and thermostability of the particles has not been evaluated. Results In the present study we demonstrate the use of a system developed to generate nano and microparticles carrying as a fusion protein peptides or proteins of interest to be used as vaccines. These particles are purified easily by centrifugation. Immunization of animals with the particles in the absence of adjuvant result in a robust and long-lasting immune response. Proteins contained inside the particles are maintained for over 1 year at ambient temperature, preserving their immunological properties. Conclusion The rapid and efficient production of the particles in addition to the robust immune response they generate position this system as an excellent method for the rapid response against emerging diseases. The thermostability conferred by the particle system facilitates the distribution of the vaccines in developing countries or areas with no electricity.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1472-6750Test

الوصول الحر: https://doaj.org/article/d1a3953b2b2b48a4a7a4569fc81e8a7cTest

المؤلفون: Daniel Martínez-Flores, Jesús Zepeda-Cervantes, Adolfo Cruz-Reséndiz, Sergio Aguirre-Sampieri, Alicia Sampieri, Luis Vaca

المصدر: Frontiers in Immunology, Vol 12 (2021)

مصطلحات موضوعية: spike glycoprotein, RBD, vaccine design, SARS-CoV-2 variants, resistance to neutralization, Immunologic diseases. Allergy, RC581-607

الوصف: Coronavirus 19 Disease (COVID-19) originating in the province of Wuhan, China in 2019, is caused by the severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), whose infection in humans causes mild or severe clinical manifestations that mainly affect the respiratory system. So far, the COVID-19 has caused more than 2 million deaths worldwide. SARS-CoV-2 contains the Spike (S) glycoprotein on its surface, which is the main target for current vaccine development because antibodies directed against this protein can neutralize the infection. Companies and academic institutions have developed vaccines based on the S glycoprotein, as well as its antigenic domains and epitopes, which have been proven effective in generating neutralizing antibodies. However, the emergence of new SARS-CoV-2 variants could affect the effectiveness of vaccines. Here, we review the different types of vaccines designed and developed against SARS-CoV-2, placing emphasis on whether they are based on the complete S glycoprotein, its antigenic domains such as the receptor-binding domain (RBD) or short epitopes within the S glycoprotein. We also review and discuss the possible effectiveness of these vaccines against emerging SARS-CoV-2 variants.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fimmu.2021.701501/fullTest; https://doaj.org/toc/1664-3224Test

الوصول الحر: https://doaj.org/article/a8665ecfee1248dc8197937d267ee864Test

المؤلفون: Rogelio Arteaga-Tlecuitl, Ana Laura Sanchez-Sandoval, Belen Ernestina Ramirez-Cordero, Margarita Jacaranda Rosendo-Pineda, Luis Vaca, Juan Carlos Gomora

المصدر: BMC Research Notes, Vol 11, Iss 1, Pp 1-9 (2018)

مصطلحات موضوعية: Voltage-gated calcium channel, CaV3 channel, Electrophysiology, β-Subunit, Protein–protein interaction, Fluorescence, Medicine, Biology (General), QH301-705.5, Science (General), Q1-390

الوصف: Abstract Objective Low voltage-activated (LVA) calcium channels are crucial for regulating oscillatory behavior in several types of neurons and other excitable cells. LVA channels dysfunction has been implicated in epilepsy, neuropathic pain, cancer, among other diseases. Unlike for High Voltage-Activated (HVA) channels, voltage-dependence and kinetics of currents carried by recombinant LVA, i.e., CaV3 channels, are quite similar to those observed in native currents. Therefore, whether these channels are regulated by HVA auxiliary subunits, remain controversial. Here, we used the α1-subunits of CaV3.1, CaV3.2, and CaV3.3 channels, together with HVA auxiliary β-subunits to perform electrophysiological, confocal microscopy and immunoprecipitation experiments, in order to further explore this possibility. Results Functional expression of CaV3 channels is up-regulated by all four β-subunits, although most consistent effects were observed with the β1b-subunit. The biophysical properties of CaV3 channels were not modified by any β-subunit. Furthermore, although β1b-subunits increased colocalization of GFP-tagged CaV3 channels and the plasma membrane of HEK-293 cells, western blots analysis revealed the absence of physical interaction between CaV3.3 and β1b-subunits as no co-immunoprecipitation was observed. These results provide solid evidence that the up-regulation of LVA channels in the presence of HVA-β1b subunit is not mediated by a high affinity interaction between both proteins.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s13104-018-3917-1Test; https://doaj.org/toc/1756-0500Test

الوصول الحر: https://doaj.org/article/bc231bdb655e45fe9d0092b40313bb8bTest

المؤلفون: Jesus Zepeda-Cervantes, Luis Vaca

المصدر: Expert Review of Vaccines, Vol 17, Iss 8, Pp 723-738 (2018)

مصطلحات موضوعية: antigen delivery system, baculovirus, polyhedrin protein, self-aggregating peptides, subunit vaccine, Internal medicine, RC31-1245

الوصف: Introduction: Recently, subunit vaccines are replacing some of the traditional vaccines because they offer a higher margin of safety. However, generally subunit vaccines have low antigenicity. Adjuvants are used in vaccine formulations to increase their immunogenicity, but current research suggests that adjuvants could induce serious side effects in susceptible individuals; therefore, the improvement of antigens and adjuvants is important. Areas covered: Here we reviewed some self-aggregating peptides (SAPs) used as antigen delivery systems. SAPs are based on a short sequence of amino acids, which have self-aggregating properties, inducing self-interaction among peptide molecules by means of non-covalent interactions to generate nanoparticles (NPs). Expert commentary: SAPs increase the immunogenicity of fused/conjugated antigens because they can interact with antigen-presenting cells and induce adaptive immunity based on both humoral and cellular responses. As an example, we report an antigen delivery system based on SAPs forming NPs. These NPs are synthesized using a recombinant baculovirus. We fused the green fluorescent protein to the first 110 amino acids of polyhedrin protein from Autographa californica nucleopolyhedrovirus, which has self-aggregating properties. We showed that these NPs prompt high antibody levels without inducing inflammation, similarly to some SAPs reported here.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1476-0584Test; https://doaj.org/toc/1744-8395Test

الوصول الحر: https://doaj.org/article/6db1dacfeb724002bba1646eb30f0490Test