المؤلفون: Yang Ru, Xiao Jianhua, Liu YingLan, Xu ShiKang

المصدر: e-Polymers, Vol 24, Iss 1, Pp 42-52 (2024)

مصطلحات موضوعية: additive manufacturing, fused deposition modeling, gas-assisted, hot air, die swell, Polymers and polymer manufacture, TP1080-1185

الوصف: For eliminating the die swell phenomenon in 3D printing of GF/PP, a hot air assisted 3D printing method is proposed and its mechanism is studied. A two-phase flow model consisting of compressible gas and in-compressible melt is established, and the process of polymer filament extrusion is simulated. A series of experiments are conducted to compare the differences between traditional printing and gas-assisted printing in terms of extruded filament, temperature, and morphology. The simulation and experiment results show that the addition of gas effectively mitigates the melt die swell, and increases the extrusion filament temperature to more than 70°C. The extrusion pressure is reduced about two orders of magnitude, and the first normal stress is decreased from 400,000 to 20,000 Pa. The surface morphology of printed product is smoother and more refined. This study provides valuable information for understanding the principles of gas-assisted printing and demonstrates its potential for improving printing quality and efficiency.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1618-7229Test

الوصول الحر: https://doaj.org/article/5c477486479b47fdbe8e3127464d0691Test

المؤلفون: Liu, Yinglan, Xiong, Ying

المصدر: Cancer Cell International ; volume 23, issue 1 ; ISSN 1475-2867

مصطلحات موضوعية: Cancer Research, Genetics, Oncology

الوصف: Background Ovarian cancer, a prevalent cause of cancer-related mortality among gynecological cancers, still lacks a clear understanding of its pathogenesis. In this study, our objective was to investigate the functional roles and pathogenic mechanisms of HADHA in ovarian cancer. Methods We utilized an ovarian cancer tissue microarray and three ovarian cancer cell lines (HO-8910, A2780, and SK-OV-3) for our analysis. Lentiviral-mediated short hairpin RNA (shRNA) was employed to interfere with HADHA expression in ovarian cancer cells. Various cellular events associated with tumor development were assessed using techniques such as Celigo cell counting assay, wound healing assay, Transwell assay, and flow cytometry analysis. Additionally, xenograft tumor models were developed to visualize the impacts of HADHA/CDK1 on ovarian cancer progression. Results Our data revealed significant HADHA overexpression in both ovarian cancer tissues and cell lines. Patients with elevated HADHA levels tended to experience poor survival outcomes. Moreover, HADHA upregulation correlated with several pathological parameters, including pathological stage, tumor size, tumor infiltrate, metastasis, and recurrence. Loss-of-function experiments targeting HADHA demonstrated that its suppression in ovarian cancer cells hindered cell growth and migration, while promoting apoptosis. To elucidate the underlying mechanism by which HADHA regulates ovarian cancer, we identified CDK1 as a target of HADHA. HADHA upregulated CDK1 expression by inhibiting its ubiquitination-dependent proteasomal degradation. Significantly, the overexpression of CDK1 reversed the impaired cell development caused by HADHA depletion, both in vitro and in vivo. Conclusion Our study highlights the involvement of HADHA in ovarian cancer tumorigenesis and suggests its potential as a promising prognostic marker in ovarian cancer. Through its regulation of CDK1, HADHA influences critical cellular processes in ovarian cancer, providing insights into its pathogenic ...

الإتاحة: https://doi.org/10.1186/s12935-023-03120-4Test

المؤلفون: Jiang, Aihua, Liu, Lin, Wang, Jianping, Liu, Yinglan, Deng, Shanshan, Jiang, Tao

المصدر: Cardiovascular Toxicology; Jun2024, Vol. 24 Issue 6, p587-597, 11p

مصطلحات موضوعية: NOTCH genes, NOTCH signaling pathway, TYPE 2 diabetes, LABORATORY rats, VASCULAR smooth muscle, PERCUTANEOUS coronary intervention

مستخلص: Vascular lesions frequently arise as complication in patients diagnosed with diabetes mellitus (DM). Presently, percutaneous coronary intervention (PCI) and antithrombotic therapy serve as primary treatments. However, in-stent restenosis persists as a challenging clinical issue following PCI, lacking sustained and effective treatment. Linarin (LN) exhibits diverse pharmacological activities and is regarded as a potential drug for treating various diseases, including DM. But its specific role in restenosis after vascular injury in DM patients remains unclear. A rat model of diabetes-related restenosis was established to evaluate the role of LN on neointimal hyperplasia. Vascular smooth muscle cells (VSMCs) stimulated by high glucose (HG, 30 mM) underwent LN treatment. Additionally, an overexpression plasmid of A disintegrin and metalloproteinases (ADAM10) was constructed to transfect VSMCs. We employed CCK-8, Brdu, wound-healing scratch, and transwell migration assays to evaluate the proliferation and migration of VSMCs. Furthermore, western blot and immunofluorescence assays were utilized to investigate the expressions of ADAM10 and the downstream Notch signaling pathway in vivo and in vitro models. LN notably alleviated intimal hyperplasia after vascular injury in DM rats and reduced the protein expression of ADAM10, alongside its downstream Notch1 signaling pathway-related proteins (Notch1, NICD and Hes1) in rat carotid artery tissues. LN effectively suppressed the proliferation and migration of VSMCs induced by HG, downregulating the protein expression of ADAM10, Notch1, NICD and Hes1. Moreover, our findings indicated that ADAM10 overexpression significantly reversed LN's effects on proliferation, migration, and the expression of Notch1 signaling pathway-related proteins in HG-treated VSMCs. LN demonstrates potential therapeutic efficacy in addressing restenosis after diabetic-related vascular injury, with the ADAM10 mediated Notch signaling pathway playing a pivotal role. [ABSTRACT FROM AUTHOR]

: Copyright of Cardiovascular Toxicology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Luo, Liang, Li, Yang, Sun, Xiong, Li, Jing, Hu, Enyuan, Liu, Yinglan, Tian, Yang, Yang, Xiao-Qing, Li, Yaping, Lin, Wen-Feng, Kuang, Yun, Liu, Wen, Sun, Xiaoming

المساهمون: Office of Science, Ministry of Education of the People's Republic of China, Capital Normal University, Ministry of Finance, Newton Fund, National Key Research and Development Program of China, Vehicle Technologies Office, Fundamental Research Funds for the Central Universities, U.S. Department of Energy, Program for Changjiang Scholars and Innovative Research Team in University, National Natural Science Foundation of China, Royal Society

المصدر: Chem ; volume 6, issue 2, page 448-459 ; ISSN 2451-9294

مصطلحات موضوعية: Materials Chemistry, Biochemistry (medical), General Chemical Engineering, Environmental Chemistry, Biochemistry, General Chemistry

الإتاحة: https://doi.org/10.1016/j.chempr.2019.11.004Test
https://api.elsevier.com/content/article/PII:S2451929419305145?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2451929419305145?httpAccept=text/plainTest

المؤلفون: Yaping Li, Liu Yinglan, Sun Xiong, Yang Tian, Wen-Feng Lin, Yun Kuang, Jing Li, Xiao-Qing Yang, Wen Liu, Xiaoming Sun, Enyuan Hu, Liang Luo, Yang Li

المصدر: Chem. 6:448-459

مصطلحات موضوعية: Nanostructure, Materials science, Passivation, General Chemical Engineering, chemistry.chemical_element, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biochemistry, Nanomaterials, Metal, Aluminium, Materials Chemistry, Environmental Chemistry, Surface plasmon resonance, Plasmon, Biochemistry (medical), General Chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, chemistry, visual_art, visual_art.visual_art_medium, 0210 nano-technology, Luminescence

الوصف: Summary The high reductivity of aluminum (Al) implies the utmost difficulty in achieving oxygen-resistant, ultrathin Al nanostructures. Herein, we demonstrate that sub-2-nm-thick Al nanosheets with ambient stability can be synthesized through a facile wet-chemical approach. Selective oxygen adsorption on the (111) facets of the face-centered cubic (fcc) Al has been revealed as the reason of controlling the morphology and stability of Al nanosheets, tailoring the thickness from 18 nm to 1.5 nm. Within the (111) surface passivation, Al nanosheets have achieved satisfactory stability, ensuring the possibility to study thickness-dependent localized surface plasmon resonance from visible to near-infrared (near-IR) region, and significantly enhanced two-photon luminescence. This work demonstrates, for the first time, the feasibility in obtaining stable ultrathin nanostructures of Al metal, which paves the way toward optical applications of Al as a sustainable plasmonic material, and shows great potential in the synthesis of other active metal-based nanomaterials.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::c8841715263f331b274aa04fe20ef761Test
https://doi.org/10.1016/j.chempr.2019.11.004Test

المؤلفون: Luo, Liang, Xie, Qixian, Liu, Yinglan

المصدر: SpringerBriefs in Molecular Science ; Nanoseparation Using Density Gradient Ultracentrifugation ; page 79-94 ; ISSN 2191-5407 2191-5415 ; ISBN 9789811051890 9789811051906

الإتاحة: https://doi.org/10.1007/978-981-10-5190-6_5Test

المؤلفون: Luo, Liang, Li, Yang, Sun, Xiong, Li, Jing, Hu, Enyuan, Liu, Yinglan, Tian, Yang, Yang, Xiao-Qing, Li, Yaping, Lin, Wen-Feng, Kuang, Yun, Liu, Wen, Sun, Xiaoming

وصف الملف: application/pdf

العلاقة: http://www.osti.gov/servlets/purl/1877703Test; https://www.osti.gov/biblio/1877703Test; https://doi.org/10.1016/j.chempr.2019.11.004Test

الإتاحة: https://doi.org/10.1016/j.chempr.2019.11.004Test
http://www.osti.gov/servlets/purl/1877703Test
https://www.osti.gov/biblio/1877703Test

المؤلفون: Liu, Yinglan, Jiang, Xiaoxin, Zeng, Yong, Zhou, Hui, Yang, Jing, Cao, Renxian

المصدر: Histochemistry and Cell Biology ; volume 142, issue 6, page 685-691 ; ISSN 0948-6143 1432-119X

مصطلحات موضوعية: Cell Biology, Medical Laboratory Technology, Molecular Biology, Histology

الإتاحة: https://doi.org/10.1007/s00418-014-1248-4Test

المؤلفون: Liu, Yinglan, Yu, Liqian, Wang, Yingwei, Zhang, Yaling, Wang, Yingchao, Zhang, Guangmei

المصدر: Journal of Cancer Research and Clinical Oncology ; volume 138, issue 4, page 647-655 ; ISSN 0171-5216 1432-1335

مصطلحات موضوعية: Cancer Research, Oncology, General Medicine

الإتاحة: https://doi.org/10.1007/s00432-011-1099-5Test

المؤلفون: Liu, Yinglan, Zhang, Yaling, Fu, Jianhuan, Tan, Wenhua

المصدر: Journal of Interferon & Cytokine Research ; volume 32, issue 5, page 191-197 ; ISSN 1079-9907 1557-7465

مصطلحات موضوعية: Virology, Cell Biology, Immunology

الإتاحة: https://doi.org/10.1089/jir.2011.0066Test