المؤلفون: Cao, Shan, Yang, Lingjie, Liu, Liyun, Mu, Yuming, Guan, Lina

المساهمون: National Natural Science Foundation of China

المصدر: BMC Cardiovascular Disorders ; volume 24, issue 1 ; ISSN 1471-2261

مصطلحات موضوعية: Cardiology and Cardiovascular Medicine

الوصف: Background To evaluate the changes of right ventricular (RV) myocardial perfusion and function in patients with hypertrophic cardiomyopathy (HCM) by myocardial contrast echocardiography (MCE) and speckle tracking (2D-STE), and to explore the relationship between RV myocardial perfusion and strain. Methods Conventional ultrasound, MCE and 2D-STE were performed on 29 HCM patients and 21 healthy subjects to analyze RV myocardial perfusion, RV global strain, RV free wall strain, and strain of each segment. The correlation between RV myocardial perfusion and strain was further analyzed in HCM patients. Results MCE results showed that the regional myocardial perfusion of the RV in HCM patients was decreased. Compared with the normal control group, the mean slope (β) in the middle and apical segments of the RV free wall, and the peak intensity (A), β, myocardial blood flow (MBF) of the ventricular septum decreased in HCM patients ( P < 0.05). RV function was impaired in HCM patients. The RV global strain (RV GLS), and the strain of RV free wall and each segment were lower than those in the normal control group ( P < 0.05). Correlation analysis showed that there was a certain correlation between RV myocardial perfusion and strain, such as the β of the whole RV in HCM group had a positive correlation with the strain of the middle segment of the interventricular septum ( r = 0.550, P = 0.002). Conclusions The regional myocardial perfusion and strain of the RV in HCM patients are reduced, and there is a positive correlation between them, suggesting that the reduction of myocardial strain may be related to the impairment of myocardial microcirculation.

الإتاحة: https://doi.org/10.1186/s12872-024-03705-5Test

المؤلفون: Liu, Liyun, Assaha, Dekoum V. M., Islam, Mohammad Sohidul, Rajendran, Karthika, Theivasigamani, Parthasarathi, Soufan, Walid, Ayman, El Sabagh, Ueda, Akihiro

المصدر: Frontiers in Sustainable Food Systems ; volume 7 ; ISSN 2571-581X

مصطلحات موضوعية: Horticulture, Management, Monitoring, Policy and Law, Agronomy and Crop Science, Ecology, Food Science, Global and Planetary Change

الوصف: Oat ( Avena nuda L.) is a globally important cereal crop grown for its nutritious grains and is considered as moderately salt-tolerant. Studying salinity tolerant mechanisms of oats could assist breeders in increasing oat production and their economic income in salt-affected areas, as the total amount of saline land in the world is still increasing. The present study was carried out to better understand the salt tolerance mechanism of the naked oat line Bayou1. A soil experiment was conducted on 17 days-old Bayou1 seedlings treated with varying concentrations of NaCl for a period of 12 days. Bayou1 plants grew optimally when treated with 50 mM NaCl, demonstrating their salinity tolerance. Reduced water uptake, decreased Ca 2+ , Mg 2+ , K + , and guaiacol peroxidase activity, as well as increased Na + concentration in leaves, all contributed to a reduction in shoot growth. However, the damage to ionic homeostasis caused by increased Na + concentrations and decreased K + concentrations in the roots of Bayou1 did not inhibit its root growth, indicating that the main salt-tolerant mechanism in Bayou1 existed in its roots. Further, a hydroponic experiment found that increasing Na + concentration in root cell sap enhanced root growth, while maintaining the integrity of root cell membranes. The accumulated Na + may have facilitated the root growth of Bayou1 exposed to NaCl by effectively adjusting cellular osmotic potential, thereby ensuring root cell turgor and expansion.

الإتاحة: https://doi.org/10.3389/fsufs.2023.1336350Test

المؤلفون: Zhu, Hongrui, Gao, Yamin, Liu, Liyun, Tao, Mengyu, Lin, Xiao, Cheng, Yijia, Shen, Yaoyao, Xue, Haitao, Guan, Li, Zhao, Huimin, Liu, Li, Wang, Shuping, Yang, Fan, Zhou, Yongjun, Liao, Hongze, Sun, Fan, Lin, Houwen

المساهمون: National Natural Science Foundation of China, School of Medicine, Shanghai Jiao Tong University, National Key Research and Development Program of China

المصدر: Acta Pharmaceutica Sinica B ; volume 14, issue 1, page 207-222 ; ISSN 2211-3835

مصطلحات موضوعية: General Pharmacology, Toxicology and Pharmaceutics

الإتاحة: https://doi.org/10.1016/j.apsb.2023.10.013Test
https://api.elsevier.com/content/article/PII:S2211383523004070?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2211383523004070?httpAccept=text/plainTest

المؤلفون: Fan, Huayu, Cao, Xiangyang, Wu, Mingxiang, Zhao, Mingyu, Liu, Liyun, Song, Yongwei, Zhang, Xiangdong, Zhang, Binqing

المصدر: Current Medical Imaging Formerly Current Medical Imaging Reviews ; volume 20 ; ISSN 1573-4056

مصطلحات موضوعية: Radiology, Nuclear Medicine and imaging

الوصف: Background:: Magnetic resonance imaging (MRI) is a handy diagnostic tool for orthopedic disorders, particularly spinal and joint diseases. Methods:: The lumbar intervertebral disc is visible in the T1 and T2 weight sequences of the spine MRI, which aids in diagnosing lumbar disc herniation, lumbar spine tuberculosis, lumbar spine tumors, and other conditions. The lumbar intervertebral disc cannot be seen accurately in the Spectral Attenuated Inversion Recovery (SPAIR) due to weaknesses in the fat and frequency offset parameters, which is not conducive to developing the intelligence diagnosis model of medical image. Results:: In order to solve this problem, we propose a composite framework, which is first to use the contrast limited adaptive histogram equalization (CLAHE) method to enhance the SPAIR image contrast of the spine MRI and then use the non-local means method to remove the noise of the image to ensure that the image contrast is uniform without losing details. We employ the Information Entropy (IE), Peak signal-to-noise ratio (PSNR), and feature similarity index measure (FSIM) to quantify image quality after enhancement by the composite framework. Conclusion:: The outcomes of the experiments’ output images and quantitative data indicate that our composite framework is better than others.

الإتاحة: https://doi.org/10.2174/0115734056251482231107072125Test
https://www.eurekaselect.com/226644/articleTest

المؤلفون: Yuan Chen, Liu Liyun, Tayier Baihetiya, Ma Ting, Guan Lina, Mu Yuming, Li Yanhong

المصدر: Open Life Sciences, Vol 18, Iss 1, Pp 920-33 (2023)

مصطلحات موضوعية: drug delivery, dual targeting, foam cells, atherosclerosis, Biology (General), QH301-705.5

الوصف: Given the miR-33’s mechanistic relationships with multiple etiological factors in the pathogenesis of atherosclerosis (AS), we investigated the therapeutic potentials of dual-targeted microbubbles (HA-PANBs) in foam cell-specific release of anti-miR-33 (ANM33) oligonucleotides, resulting in the early prevention of AS progression and severity. The intracellular localization, loading optimization, and therapeutic effects of HA-PANBs were examined in detail in a co-cultured cell model of phagocytosis. Compared with non-targeting nanobubbles (NBs) and single-targeted microbubbles as controls, HA-PANBs efficiently delivered the ANM33 specifically to foam cells via sustained release, exhibiting its clinical value in mediating RNA silencing. Moreover, when used at a dose of 12 µg/mL HA-PANBs per 107 cells for 48 h, a higher release rate and drug efficacy were observed. Therefore, HA-PANBs, effectively targeting early AS foam cells, may represent a novel and optimal gene therapy approach for AS management.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2391-5412Test

الوصول الحر: https://doaj.org/article/967e1417fe864c4fa5e94d165ada7491Test

المؤلفون: Mohammad Sohidul Islam, Md. Kamrul Hasan, Md. Rafiqul Islam, Md. Kaium Chowdhury, Moaz Hosen Pramanik, Muhammad Aamir Iqbal, Karthika Rajendran, Rashid Iqbal, Walid Soufan, Muhammad Kamran, Liu Liyun, Ayman El Sabagh

المصدر: Frontiers in Ecology and Evolution, Vol 11 (2023)

مصطلحات موضوعية: gibberellic acid (GA3), growth regulators, water relations, grain yield, functional relationship, mungbean, Evolution, QH359-425, Ecology, QH540-549.5

الوصف: Optimizing the dose and foliar application frequency of growth regulators such as gibberellic acid (GA3) may play an important role in ensuring food security under changing climate scenarios by boosting grain yield of food legumes such as mungbean. A trial was conducted to evaluate the growth promoting effect of foliage applied gibberellic acid (GA3) at various spraying frequencies for mungbean crop. The employed treatments included four gibberellic acid levels (0, 100, 200, and 300 ppm) and two application frequencies (single spray at 30 days after sowing DAS, and two sprays at 30 and 40 DAS). Water relations, yield contributing characteristics and mungbean grain yield were among the response variables investigated. The research findings revealed that GA3 (200 ppm applied twice at pre-flowering and post-flowering stages) significantly improved the water relations, morphological and yield attributes of mungbean. This treatment combination remained unmatched by producing the highest relative water content in the stem (85.52), water retention capacity in the stem (17.24), and water uptake capacity in the stem and leaf (2.35). Furthermore, the same treatment combination resulted in the maximum plant height (50.04 cm), pods per plant (11.07), pod length (6.62 cm), grains number per pod (11.00), 100-grains weight (3.78 g), grain yield per plant (4.57 g), and a minimum water saturation deficit. Thus, GA3 foliage application at 200 ppm at 30 and 60 DAS has the potential to stimulate growth and increase the yield attributes and grain yield of mungbean. However, additional in-depth field trials with various doses of GA3 sprayed at a higher frequency may be required before recommending GA3 for general adoption to mungbean growers.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fevo.2023.1048768/fullTest; https://doaj.org/toc/2296-701XTest

الوصول الحر: https://doaj.org/article/edc63623b0884911bd84a30ff408b123Test

المؤلفون: Ding, Ruixue, Yang, Shanshan, Geng, Lijuan, Liu, Yumeng, He, Baoping, Liu, Liyun, Yue, Xiqing, Wu, Rina, Wu, Junrui

المصدر: Food Science and Human Wellness ; volume 12, issue 4, page 1279-1286 ; ISSN 2213-4530

الإتاحة: https://doi.org/10.1016/j.fshw.2022.10.010Test
https://api.elsevier.com/content/article/PII:S2213453022002294?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2213453022002294?httpAccept=text/plainTest

المؤلفون: An, Kang, Zhang, Ruohan, Zhu, Binlu, Liu, Liyun, Tang, Jiayu, Ma, Yiru, Wu, Zengxiang, Zhang, Lin, She, Yi, Luo, Dan, Li, Caizheng, Li, Heting, Ma, Yuehua, Shi, Weichang, Su, Qiaoli, Li, Shuangqing

المساهمون: National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Sichuan Science and Technology Program

المصدر: BMC Medical Education ; volume 23, issue 1 ; ISSN 1472-6920

مصطلحات موضوعية: Education, General Medicine

الوصف: Background The insufficient number of general practitioners (GPs) is a major challenge facing China’s healthcare system. The purpose of the GP transfer training programme was to provide training for experienced doctors to transition to general practice. However, research on the competencies of GP transfer training trainers in teaching skills in China is limited. This cross-sectional study aimed to examine the baseline familiarity with teaching skills among Chinese GP transfer training trainers. Methods An online survey was conducted among trainers who participated in the 2021 Sichuan Province General Practice Training Trainer Program. The survey collected data on participants’ characteristics and familiarity with 20 skills in three essential teaching knowledge areas: the core functions of primary care (five questions), preparation for lesson plan (four questions), and teaching methods (11 questions). Results In total, 305 participants completed the survey. Familiarity rates were generally low across all three essential teaching knowledge areas. No significant differences were observed in familiarity rates between the tertiary and secondary hospitals. Conclusion This study revealed gaps in the teaching skills of GP transfer training trainers in China. These results suggest the necessity for targeted training programs to enhance the teaching skills and competencies of trainers.

الإتاحة: https://doi.org/10.1186/s12909-023-04945-3Test

المؤلفون: Yan, Limin, Xu, Jinheng, Liu, Liyun, Li, Xin

المصدر: Science Progress ; volume 106, issue 2 ; ISSN 0036-8504 2047-7163

مصطلحات موضوعية: Multidisciplinary

الوصف: Warthin tumor (WT)-like mucoepidermoid carcinoma resembles the histologic pattern of WT and pathologists unaware of this possibility may misdiagnose it as WT with squamous and mucous epithelium metaplasia or WT malignant transfer into mucoepidermoid carcinoma. The present study reported a case of a 41-year-old Chinese female with a solitary mass in the left parotid gland. In this case, microscopic observation revealed prominent lymph node stroma and multiple cystic structures similar to those seen in WT. However, it lacked the two layers of oncocytic epithelial tissue characteristic of WT. Furthermore, fluorescence in situ hybridization detected MAML2 rearrangement in the case. Considering the histological findings, this case was diagnosed as WT-like mucoepidermoid carcinoma. The present case report provides pathological and clinical features to differentiate it from WT malignant transition into mucoepidermoid carcinoma, WT with squamous and mucous epithelium metaplasia and non-sebaceous lymphadenoma-like mucoepidermoid carcinoma. In conclusion, WT-like mucoepidermoid carcinoma as a special subtype of mucoepidermoid carcinoma has special histological characteristics, which required further observations and more case reports to clearly define this variant.

الإتاحة: https://doi.org/10.1177/00368504231179816Test

المؤلفون: Yang, Sen, Liu, Liyun, Liu, Xiaoyun, Li, Xinghua, Zheng, Yuyu, Ren, Zeen, Wang, Ruijiang, Wang, Yun, Li, Qian

المصدر: Medicine ; volume 102, issue 46, page e36046 ; ISSN 0025-7974 1536-5964

الوصف: Background: Abnormalities in the mitochondrial energy metabolism pathways are closely related to the occurrence and development of many cancers. Furthermore, abnormal genes in mitochondrial energy metabolism pathways may be novel targets and biomarkers for the diagnosis and treatment of osteosarcoma. In this study, we aimed to establish a mitochondrial energy metabolism-related gene signature for osteosarcoma prognosis. Methods: We first obtained differentially expressed genes based on the metastatic status of 84 patients with osteosarcoma from the TARGET database. After Venn analysis of differentially expressed genes and mitochondrial energy metabolism pathway-related genes (MMRGs), 2 key genes were obtained using univariate Cox regression and least absolute shrinkage and selection operator (LASSO) regression analysis. Next, we used these 2 genes to establish a prognostic signature. Subsequent analyses elucidated the correlation between these 2 key genes with clinical features and 28 types of immune cells. Pathway changes in osteosarcoma pathogenesis under different metastatic states were clarified using gene set enrichment analysis (GSEA) of differentially expressed genes. Results: A gene signature composed of 2 key prognosis-related genes (KCNJ5 and PFKFB2) was identified. A risk score was calculated based on the gene signature, which divided osteosarcoma patients into low- or high-risk groups that showed good and poor prognosis, respectively. High expression of these 2 key genes is associated with low-risk group in patients with osteosarcoma. We constructed an accurate nomogram to help clinicians assess the survival time of patients with osteosarcoma. The results of immune cell infiltration level showed that the high-risk group had lower levels of immune cell infiltration. GSEA revealed changes in immune regulation and hypoxia stress pathways in osteosarcoma under different metastatic states. Conclusion: Our study identified an excellent gene signature that could be helpful in improving the prognosis of ...

الإتاحة: https://doi.org/10.1097/md.0000000000036046Test