المؤلفون: Guangqi An, Bo Lei, Zhili Wang, Kaizhuan Yang, Dongsheng Fan, Bing Li, Ke Fu, Haixin Fang, Min Zhang, Lin Li, Yu Zhao, Xuemin Jin, Liping Du

المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024)

مصطلحات موضوعية: Retina, COVID-19, Acute macular neuroretinopathy, Multimodal Imaging, SARS-CoV-2, Medicine, Science

الوصف: Abstract To define the characteristics of fundus manifestations in patients after SARS-CoV-2 infection with multimodal imaging techniques. This is a retrospective multicenter and multimodal imaging study including 90 patients. All patients with a visual complaint occurring immediately after SARS-CoV-2 infection were referred to six clinics between December 2022 and February 2023. Demographic information and the temporal relationship between SARS-CoV-2 infection and visual symptoms were documented. The characteristics of the fundus lesions were evaluated using multimodal imaging. Ninety patients from six hospitals were included in this study, including 24 males (26.67%) and 66 (73.33%) females. Seventy-eight patients (86.66%) (146 eyes) were diagnosed with Acute Macular Neuroretinopathy (AMN). The AMN patients were primarily young women (67.95%). Sixty-eight patients (87.18%) had AMN in both eyes. Thirty-eight eyes (24.36%) included Purtscher or Purtscher-like lesions. optical coherence tomography and infrared retinal photographs can show AMN lesions well. Eleven cases were diagnosed with simple Purtscher or Purtscher-like retinopathy (2 cases, 2.22%), Vogt‒Koyanagi‒Harada (VKH) syndrome or VKH-like uveitis (3 cases, 3.33%), multiple evanescent white-dot syndrome (MEWDS) (2 cases, 2.22%), and rhino-orbital-cerebral mucormycosis (ROCM) (5 cases, 5.56%). After SARS-CoV-2 infection, diversified fundus lesions were evident in patients with visual complaints. In this report, AMN was the dominant manifestation, followed by Purtscher or Purtscher-like retinopathy, MEWDS, VKH-like uveitis, and ROCM.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-2322Test

الوصول الحر: https://doaj.org/article/2d813993f753428aadcf40260a46d74aTest

المؤلفون: Lin Li, Fuzhen Li, Jiankang Shan, Kunpeng Xie, Pengyi Zhou, Haiyan Zhu, Xuemin Jin, Liping Du, Peizeng Yang

المصدر: BMC Ophthalmology, Vol 24, Iss 1, Pp 1-6 (2024)

مصطلحات موضوعية: Acute anterior uveitis, IL6 receptor, IL10, Genetic variation, Ophthalmology, RE1-994

الوصف: Abstract Background Several autoimmune disorders have been linked to polymorphisms in IL10 and IL6R genes. This research aimed to study whether single nucleotide polymorphisms (SNPs) in the genes of IL10 and IL6R were associated with acute anterior uveitis (AAU) in Han Chinese. Methods Genotyping was carried out by the iPLEX Gold Genotyping Assay. Our study comprised 420 patients with AAU and 918 healthy subjects from Han Chinese. Using the chi-square (χ2) test, alleles and genotypes were analyzed between AAU subjects and healthy controls. Results All ten SNPs were successfully genotyped and four SNPs (IL10/rs1800871, IL10/rs3021094, IL10/rs2222202, IL6R/rs4845618) exhibited weak associations with AAU, as indicated by their Puncorr values. However, upon applying the Bonferroni correction, there was no significant association between AAU and the control subjects. Additionally, the haplotype analysis of the ten SNPs revealed no association with AAU. Conclusion Our findings suggested that polymorphisms of the tested ten SNPs on the IL10 and IL6R genes did not show any association with the risk of developing AAU among the Han Chinese population.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2415Test

الوصول الحر: https://doaj.org/article/f1b1bf0b2865410bb238e87af815c3cfTest

المؤلفون: Zhizhong Tian, Linxia Liu, Lijuan Wu, Zixuan Yang, Yahui Zhang, Liping Du, Dawei Zhang

المصدر: Microbial Cell Factories, Vol 23, Iss 1, Pp 1-15 (2024)

مصطلحات موضوعية: Vitamin B6, Omics analysis, Cellular processes, Fermentation optimization, Microbiology, QR1-502

الوصف: Abstract Background Microbial engineering aims to enhance the ability of bacteria to produce valuable products, including vitamin B6 for various applications. Numerous microorganisms naturally produce vitamin B6, yet the metabolic pathways involved are rigorously controlled. This regulation by the accumulation of vitamin B6 poses a challenge in constructing an efficient cell factory. Results In this study, we conducted transcriptome and metabolome analyses to investigate the effects of the accumulation of pyridoxine, which is the major commercial form of vitamin B6, on cellular processes in Escherichia coli. Our omics analysis revealed associations between pyridoxine and amino acids, as well as the tricarboxylic acid (TCA) cycle. Based on these findings, we identified potential targets for fermentation optimization, including succinate, amino acids, and the carbon-to-nitrogen (C/N) ratio. Through targeted modifications, we achieved pyridoxine titers of approximately 514 mg/L in shake flasks and 1.95 g/L in fed-batch fermentation. Conclusion Our results provide insights into pyridoxine biosynthesis within the cellular metabolic network for the first time. Our comprehensive analysis revealed that the fermentation process resulted in a remarkable final yield of 1.95 g/L pyridoxine, the highest reported yield to date. This work lays a foundation for the green industrial production of vitamin B6 in the future.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1475-2859Test

الوصول الحر: https://doaj.org/article/d9813ec862dd4374bca6b4b1a6e93d75Test

المؤلفون: Pei Liu, Haixin Fang, Guangqi An, Bo Jin, Chenyu Lu, Shu Li, Fan Yang, Liping Du, Xuemin Jin

المصدر: Ophthalmology and Therapy, Vol 13, Iss 1, Pp 321-335 (2023)

مصطلحات موضوعية: Chronic central serous chorioretinopathy, Vascular density, Subfoveal choroidal thickness, Ellipsoid zone rupture, Pachydrusen, Outer nuclear layer, Ophthalmology, RE1-994

الوصف: Abstract Introduction With advancements in imaging technology, researchers have been able to identify more distinctive imaging features of central serous chorioretinopathy (CSC). However, existing research primarily concentrates on young patients aged 50 years and below, leaving a dearth of studies on elderly CSC patients. Previous studies indicate that elderly CSC patients may exhibit unique imaging characteristics and have a clinical prognosis that significantly differs from younger patients. This study aimed to evaluate the characteristics of retina, choroid structure, and blood flow in elderly patients with chronic CSC (cCSC) examined multimode imaging and try to find new pathogenesis information of it. Methods Using a cut-off age of 50 years, patients with chronic central serous chorioretinopathy were divided into two groups: older and younger. The control group consisted of 40 healthy individuals, with their right eyes assigned. Various clinical features were recorded, including the incidence of ellipsoid zone rupture (EZ-), fibrin in the subretinal fluid (SRF), pachydrusen, subretinal drusenoid deposits (SDD), pigment epithelial detachment (PED), double-layer sign (DLS), and choroidal lipid globule cavern. Measurements were taken for the thickness of the outer nuclear layer (ONL), the length of the extended outer photoreceptor segment (POS), the height and width of SRF, the vascular density of each layer of the retinal capillary plexus, the central macular thickness (CMT), and the subfoveal choroidal thickness (SFCT). Results The proportion of females in the elderly group (43.75%) was significantly higher than that in the youth group (22.41%) (p = 0.034). The degree of hyperopia in the elderly group (1.03 ± 0.73) was higher than that in the youth group (0.26 ± 1.06), with a significant difference in BCVA (p = 0.05). The thickness of SFCT, CMT, ONL in the elderly group, and the length of photoreceptor outer segment in the elderly group were thinner than those in the youth group (p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2193-8245Test; https://doaj.org/toc/2193-6528Test

الوصول الحر: https://doaj.org/article/566345b1eb884ed7965b54d3ede5374eTest

المؤلفون: Guang Chen, Yueyun Chen, Zhiyuan Mai, Conghui Hao, Meijie Yang, Shuangshuang Han, Liping Du

المصدر: Digital Communications and Networks, Vol 9, Iss 3, Pp 613-627 (2023)

مصطلحات موضوعية: Mobile edge computing, Intelligent reflective surface, Task offloading, Multiple resource allocation, Non-orthogonal multiple access, Information technology, T58.5-58.64

الوصف: Effective resource allocation can exploit the advantage of intelligent reflective surface (IRS) assisted mobile edge computing (MEC) fully. However, it is challenging to balance the limited energy of MTs and the strict delay requirement of their tasks. In this paper, in order to tackle the challenge, we jointly optimize the offloading delay and energy consumption of mobile terminals (MTs) to realize the delay-energy tradeoff in an IRS-assisted MEC network, in which non-orthogonal multiple access (NOMA) and multiantenna are applied to improve spectral efficiency. To achieve the optimal delay-energy tradeoff, an offloading cost minimization model is proposed, in which the edge computing resource allocation, signal detecting vector, uplink transmission power, and IRS phase shift coefficient are needed to be jointly optimized. The optimization of the model is a multi-level fractional problem in complex fields with some coupled high dimension variables. To solve the intractable problem, we decouple the original problem into a computing subproblem and a wireless transmission subproblem based on the uncoupled relationship between different variable types. The computing subproblem is proved convex and the closed-form solution is obtained for the edge computing resource allocation. Further, the wireless transmission subproblem is solved iteratively through decoupling the residual variables. In each iteration, the closed-form solution of residual variables is obtained through different successive convex approximation (SCA) methods. We verify the proposed algorithm can converge to an optimum with polynomial complexity. Simulation results indicate the proposed method achieves average saved costs of 65.64%, 11.24%, and 9.49% over three benchmark methods respectively.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2352864822002383Test; https://doaj.org/toc/2352-8648Test

الوصول الحر: https://doaj.org/article/6fe6cd9e9a754f1c9446608f063ab416Test

المؤلفون: Huan Liu, Panpan Zhang, Fuzhen Li, Xiao Xiao, Yinan Zhang, Na Li, Liping Du, Peizeng Yang

المصدر: Arthritis Research & Therapy, Vol 25, Iss 1, Pp 1-13 (2023)

مصطلحات موضوعية: Behcet’s disease, Uveitis, Immune, Proteomics, Biomarker, Diseases of the musculoskeletal system, RC925-935

الوصف: Abstract Background This study aimed to investigate the expression profile of immune response-related proteins of Behcet’s disease (BD) patients and identify potential biomarkers for this disease. Methods Plasma was collected from BD patients and healthy controls (HC). Immune response-related proteins were measured using the Olink Immune Response Panel. Differentially expressed proteins (DEPs) were used to construct prediction models via five machine learning algorithms: naive Bayes, support vector machine, extreme gradient boosting, random forest, and neural network. The prediction performance of the five models was assessed using the area under the curve (AUC) value, recall (sensitivity), specificity, precision, accuracy, F1 score, and residual distribution. Subtype analysis of BD was performed using the consensus clustering method. Results Proteomics results showed 43 DEPs between BD patients and HC (P

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1478-6362Test

الوصول الحر: https://doaj.org/article/647fb5ab03af4c10a2a22db59927c2feTest

المؤلفون: Rachel S. Chang, Jaimie Z. Shing, Jennifer C. Erves, Liping Du, Tatsuki Koyama, Stephen Deppen, Alyssa B. Rentuza, Caree McAfee, Christine Stroebel, Janet Cates, Lora Harnack, David Andrews, Robert Bramblett, Pamela C. Hull

المصدر: BMC Medical Informatics and Decision Making, Vol 22, Iss 1, Pp 1-15 (2022)

مصطلحات موضوعية: Measurement, Fidelity, Clinical guidelines, Assessment and feedback, Vaccine refusal, Computer applications to medicine. Medical informatics, R858-859.7

الوصف: Abstract Background Assessment and feedback is a common implementation strategy to improve healthcare provider fidelity to clinical guidelines. For immunization guidelines, fidelity is often measured with doses administered during eligible visits. Adding a patient refusal measure captures provider fidelity more completely (i.e., all instances of a provider recommending a vaccine, resulting in vaccination or refusal) and enables providers to track patient vaccine hesitancy patterns. However, many electronic health record (EHR) systems have no structured field to document multiple instances of refusals for specific vaccines, and existing billing codes for refusal are not vaccine specific. This study assessed the feasibility of a novel method for refusal documentation used in a study focused on human papillomavirus (HPV) vaccine. Methods An observational, descriptive-comparative, mixed-methods study design was used to conduct secondary data analysis from an implementation-effectiveness trial. The parent trial compared coach-based versus web-based practice facilitation, including assessment and feedback, to increase HPV vaccination in 21 community-based private pediatric practices. Providers were instructed to document initial HPV vaccine refusals in the EHR's immunization forms and subsequent refusals using dummy procedure codes, for use in assessment and feedback reports. This analysis examined adoption and maintenance of the refusal documentation method during eligible well visits, identified barriers and facilitators to documentation and described demographic patterns in patient refusals. Results Seven practices adopted the refusal documentation method. Among adopter practices, documented refusals started at 2.4% of eligible well visits at baseline, increased to 14.2% at the start of implementation, peaked at 24.0%, then declined to 18.8%. Barriers to refusal documentation included low prioritization, workflow integration and complication of the billing process. Facilitators included high motivation, documentation instructions and coach support. Among adopter practices, odds of refusing HPV vaccine were 25% higher for patients aged 15–17 years versus 11–12 years, and 18% lower for males versus females. Conclusions We demonstrated the value of patient refusal documentation for measuring HPV vaccination guideline fidelity and ways that it can be improved in future research. Creation of vaccine-specific refusal billing codes or EHR adaptations to enable documenting multiple instances of specific vaccine refusals would facilitate consistent refusal documentation. Trial Registration NCT03399396 Registered in ClinicalTrials.gov on 1/16/2018.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1472-6947Test

الوصول الحر: https://doaj.org/article/eb9d9ba2c9f44e4c857d94621d70c0ddTest

المؤلفون: Shiao Yu, Liping Du, Na Ji, Binbin Li, Xuena Pang, Xiuhong li, Nana Ma, Congcong Huang, Aicun Fu

المصدر: BMC Ophthalmology, Vol 22, Iss 1, Pp 1-9 (2022)

مصطلحات موضوعية: Orthokeratology, 0.01% atropine, Additive effects, Axial length, Myopia, Ophthalmology, RE1-994

الوصف: Abstract Background: To evaluate the additive effects of orthokeratology (OK) lenses and 0.01% atropine on slowing axial elongation in myopic children. Methods: A prospective, randomized, double-blinded, placebo-controlled trial was conducted over a 12-month period. Sixty children aged 8 to 12 years with spherical equivalent refraction from − 1.00 to -4.00 D who had been wearing OK lenses successfully for 2 months (as baseline) were randomly assigned in a 1:1 ratio to combination group (combination of OK lens and 0.01% atropine eye drops) and control group (combination of OK lens and placebo). The primary outcome was change in axial length, along with secondary outcomes including change in pupil diameter (PD) and accommodative amplitude (AMP) at 12 months (measured at 4-month intervals). Results: After 12 months, the overall axial elongation was 0.10 ± 0.14 mm and 0.20 ± 0.15 mm (p = 0.01) in the combination and control groups, respectively. The change in axial length in the two groups showed significant differences only in the first four months (median [Q1, Q3] (95% CI), -0.01 mm [-0.07, 0.05] (-0.06, 0.04) vs. 0.04 mm [0.00, 0.10] (0.02, 0.09); p = 0.04), but no difference thereafter. Multivariate linear regression analysis showed that the axial elongation was significantly slower in the combination group than in the control group (standard β = -0.10, p = 0.02). PD significantly increased by 0.45 mm [0.20, 0.68] at the 4th month visit (p 0.05). Conclusion: The combination therapy was more effective than the OK lens alone in slowing axial elongation after 12 months of treatment, and mainly in the first 4 months. Trial registration: The First Affiliated Hospital of Zhengzhou University, ChiCTR2000033904. Registered 16/06/2020, http://www.chictr.org.cn/login.aspx?referurl=%2flistbycreater.aspxTest

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2415Test

الوصول الحر: https://doaj.org/article/d7720e4dff264252bb98e5e6e4b9f0ebTest

المؤلفون: Pulak Goswami, Oladapo R. Adeniran, Shelby K. Frantz, Lea Matsuoka, Liping Du, Ripal T. Gandhi, Zachary S. Collins, Marc R. Matrana, Michael Petroziello, Jayson S. Brower, Daniel Y. Sze, Andrew S. Kennedy, Jafar Golzarian, Eric A. Wang, Daniel B. Brown

المصدر: BMC Gastroenterology, Vol 22, Iss 1, Pp 1-10 (2022)

مصطلحات موضوعية: Carcinoma, hepatocellular carcinoma, Yttrium radioisotopes/ therapeutic use, Yttrium radioisotopes/ adverse events, Adult, Treatment outcome, Diseases of the digestive system. Gastroenterology, RC799-869

الوصف: Abstract Introduction National Comprehensive Cancer Network HCC guidelines recommend Y90 to treat BCLC-C patients only in select cases given the development of systemic regimens. We sought to identify ideal candidates for Y90 by assessing survival and toxicities in this patient group. Materials and methods The Radiation-Emitting Selective Internal radiation spheres in Non-resectable tumor registry is a prospective observational study (NCT: 02,685,631). Patients with advanced HCC were stratified into 3 groups based on tumor location, Eastern Cooperative Oncology Group (ECOG) performance status, and liver function. Group 1: liver isolated HCC, ECOG 0 and Child Pugh (CP) A (n = 12, 16%), Group 2: liver isolated HCC, ECOG ≥ 1 or CP B/C (n = 37, 49%), and Group 3: extrahepatic HCC with any ECOG or CP score (n = 26, 35%). Patients in any group could have macrovascular invasion. Overall survival (OS) and progression-free survival (PFS) with 95% confidence intervals (95% CI) were calculated. Grade 3 + toxicities were tracked using Common Terminology Criteria for Adverse Events v5. Cox proportional hazard model was performed to determine factors affecting OS. Results Seventy-five BCLC-C patients treated between 2015 and 2019 were reviewed. The groups were similar in age, sex, race, and ethnicity (all p > 0.05). Bilobar disease was least common in Group 1 (p 0.05). Cox Proportional Hazard analysis predicted shorter OS with CP class B/C (X2 = 6.7, p = 0.01), while macrovascular invasion (X2 = 0.5, p = 0.5) and ECOG score of ≥ 1 (X2 = 2.1, p = 0.3) was not associated with OS. Conclusions OS of CPA patients with advanced HCC and performance status of 0 was 21.8 months following Y90. CP A cirrhosis is the best predictor of prolonged OS in advanced (BCLC-C) HCC.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-230XTest

الوصول الحر: https://doaj.org/article/42344a2ef6bc45c49359b435eddfd9f2Test

المؤلفون: Sibo Zhao, Jia Li, Huiyuan Zhang, Lin Qi, Yuchen Du, Mari Kogiso, Frank K. Braun, Sophie Xiao, Yulun Huang, Jianfang Li, Wan-Yee Teo, Holly Lindsay, Patricia Baxter, Jack M. F. Su, Adekunle Adesina, Miklós Laczik, Paola Genevini, Anne-Clemence Veillard, Sol Schvartzman, Geoffrey Berguet, Shi-Rong Ding, Liping Du, Clifford Stephan, Jianhua Yang, Peter J. A. Davies, Xinyan Lu, Murali Chintagumpala, Donald William Parsons, Laszlo Perlaky, Yun-Fei Xia, Tsz-Kwong Man, Yun Huang, Deqiang Sun, Xiao-Nan Li

المصدر: Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)

مصطلحات موضوعية: Science

الوصف: While recurrence is frequent in ependymoma, the underlying molecular mechanisms remain to be explored. Here, the authors investigate epigenetic, genetic and tumorigenic changes in 30 patient-matched repeated relapses over 13 years and identify distinct patterns of DNA methylation.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2041-1723Test

الوصول الحر: https://doaj.org/article/67ce4ebc1c4e4e89b80aca05d5ec1d5fTest