المؤلفون: Du, Jingli, Su, Yue, Qiao, Juan, Gao, Shang, Dong, Enjun, Wang, Ruilan, Nie, Yanhui, Ji, Jing, Wang, Zhendong, Liang, Jianqin, Gong, Wenping

المصدر: Chinese Medical Journal ; volume 137, issue 5, page 559-561 ; ISSN 0366-6999 2542-5641

الإتاحة: https://doi.org/10.1097/cm9.0000000000003018Test

المؤلفون: Mi, Jie, Liu, Yinping, Xue, Yong, Sun, Wenna, Liang, Yan, Liang, Jianqin, An, Huiru, Wu, Xueqiong

المصدر: Frontiers in Microbiology ; volume 15 ; ISSN 1664-302X

مصطلحات موضوعية: Microbiology (medical), Microbiology

الوصف: Objective Tuberculous meningitis (TBM) is the most severe form of tuberculosis (TB). The purpose of this study was to explore the relationship between the number of natural killer (NK) cells and adaptive immune status, and disease severity in TBM patients. Methods We conducted a retrospective study on 244 TB patients and 146 healthy control subjects in the 8th Medical Center of the PLA General Hospital from March 2018 and August 2023. Results The absolute count of NK cells in the peripheral blood of TBM patients was significantly lower than that in normal controls (NC), latent tuberculosis infection (LTBI), and non-severe TB (NSTB) patients ( p < 0.05). The proportion of TBM patients (48.7%) with a lower absolute count of NK cells than the normal reference value was significantly higher than that in NC (5.2%) and LTBI groups (4.0%) ( p < 0.05), and slightly higher than that in NSTB group (36.0%) ( p > 0.05). The absolute counts of lymphocyte subsets in TBM combined with other active TB group, etiology (+) group, IGRA (−) group, and antibody (+) group were lower than that in simple TBM group, etiology (−) group, IGRA (+) group, and antibody (−) group, respectively. The CD3 + T, NK, and B cells in BMRC-stage III TBM patients were significantly lower than those in stage I and stage II patients ( p < 0.05). The counts of CD3 + T, CD4 + T, and B cells in the etiology (+) group were significantly lower than those in the etiology (−) group ( p < 0.05). Conclusion The absolute counts of lymphocyte subsets in the peripheral blood of TBM patients were significantly decreased, especially in NK cells. The reduction of these immune cells was closely related to the disease severity and had a certain correlation with cellular and humoral immune responses. This study helps to better understand the immune mechanism of TBM and provides reliable indicators for evaluating the immune status of TBM patients in clinical practice.

الإتاحة: https://doi.org/10.3389/fmicb.2024.1344162Test

المؤلفون: Du, Jingli, Su, Yue, Dong, Enjun, Qiao, Juan, Wang, Ruilan, Zhao, Wenjuan, Liang, Jianqin, Gong, Wenping

المصدر: Frontiers in Public Health ; volume 11 ; ISSN 2296-2565

مصطلحات موضوعية: Public Health, Environmental and Occupational Health

الوصف: Background Tuberculosis (TB) prevention and control among groups living together, such as students, workers, older adults in nursing homes, and prisoners, present many challenges due to their particular age and environmental factors, which can make them more susceptible to TB clusters with significant societal impact. This study aimed to evaluate a TB cluster outbreak epidemic in a university and provide suggestions for improving TB control strategies for groups living together. Methods Pulmonary TB screening and close-contact investigation were conducted using acid-fast staining, sputum culture, GeneXpert testing, tuberculin skin testing (TST), interferon-gamma release assay (IGRA), and chest computed tomography (CT). GraphPad Prism 9.5.1 was utilized for data analysis. Collected epidemic data were comprehensively analyzed by rate comparison. Results The TB cluster outbreak epidemic was identified with an index case confirmed positive. The initial screening was conducted on potential close contacts of the index case, and the TST’s positive rate (diameter ≥ 5 mm) and strong positive rate (diameter ≥ 15 mm) among these close contacts were 65.60% (21/32) and 34.40% (11/32), respectively. Moreover, the latent TB infection (LTBI) rate (diameter ≥ 10 mm) was 43.75% (14/32), and the IGRA’s positive rate was 9.30% (3/32). Chest CT scans did not reveal any abnormalities. Surprisingly, 5 of the close contacts developed active TB in the second screening, accompanied by changes from negative to positive TST and/or IGRA results, after 3 months of follow-up. Accordingly, we expanded the screening scope to include another 28 general contacts. We found that the positive rate (78.00%, 25/32), strong positive rate (50.00%, 16/32), and LTBI rate (62.50%, 20/32) of the 32 close contacts were significantly higher than those of the additional general contacts (28.00%, 8/28; 14.3%, 4/28; 25.00%, 7/28), as indicated by p < 0.05. Conclusion In the event of an epidemic TB outbreak, it is essential to rapidly identify the source ...

الإتاحة: https://doi.org/10.3389/fpubh.2023.1136355Test

المؤلفون: An, Huiru, Sun, Wenna, Liu, Xiao, Wang, Tianhao, Qiao, Juan, Liang, Jianqin

المساهمون: She, Rosemary C., Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support

المصدر: Microbiology Spectrum ; volume 11, issue 5 ; ISSN 2165-0497

الوصف: A novel oxazolidinone for the treatment of Mycobacterium tuberculosis has been developed, but the activity of contezolid (MRX-I) still needs to be clarified. In this study, we isolated Mycobacterium tuberculosis from 48 clinical patients with pulmonary tuberculosis. Roche drug susceptibility tests identified drug-sensitive and 39 drug-resistant M. tuberculosis isolates. Drug susceptibility assays indicated that MRX-I exhibited anti- Mycobacterium tuberculosis activity against both drug-sensitive and drug-resistant isolates, with an advantage against drug-resistant isolates. The results also showed that the anti- Mycobacterium tuberculosis activity was comparable to that of linezolid. IMPORTANCE Currently, Mycobacterium tuberculosis has exhibited increased drug resistance, leading to ineffective drug treatment in many patients with tuberculosis. Among the anti- Mycobacterium tuberculosis drugs, oxazolidinones have been gradually developed. Contezolid (MRX-I) has been newly developed in China with advantages versus the first oxazolidinone antibiotic approved by the Food and Drug Administration for clinical use, but the anti- M. tuberculosis activity needs to be further clarified. In this study, in vitro activities of MRX-I against M. tuberculosis were tested. The drug susceptibility assays indicated that MRX-I exhibited anti- M. tuberculosis activity comparable to that of linezolid, with an advantage against drug-resistant isolates.

الإتاحة: https://doi.org/10.1128/spectrum.04627-22Test

المؤلفون: Zhang, Jianyou, Ji, Shengqiang, Liang, Jianqin, Chen, Yutong, Tang, Wei, Lyu, Fei

المصدر: International Journal of Food Science & Technology; Jul2024, Vol. 59 Issue 7, p4374-4392, 19p

مصطلحات موضوعية: CHEMICAL modification of proteins, ULTRASONICS, PROTEIN structure, THREE-dimensional printing

مستخلص: Summary: The advancement of the modern food industry has prompted a shift away from traditional protein extraction methods due to their inherent limitations. As a result, there is a growing interest in exploring alternative methods for protein extraction and modification such as ultrasonic (US) treatment. This shift towards more sustainable and efficient protein extraction methods is a key research focus in obtaining safe proteins in an environmentally friendly manner. Furthermore, enhancing the functional properties of proteins through modification can expand their application. This paper reviews recent research progress and applications of US treatment on protein extraction and modification as well as the impact on protein structure and functional properties. In addition, the latest applications of ultrasound‐modified‐proteins, including nano‐proteins, 3D printing and interactions between proteins and other food components, are reviewed. This review may provide some inspiration for the potential application of US technologies in food fields. [ABSTRACT FROM AUTHOR]

: Copyright of International Journal of Food Science & Technology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Cui, Pengbo, Liang, Jianqin, Cheng, Tianyu, Zhang, Jianyou

المصدر: Foods; Jun2024, Vol. 13 Issue 11, p1652, 13p

مصطلحات موضوعية: CYTOSKELETAL proteins, MACKERELS, CALCIUM ions, PEPTIDES, CALCIUM supplements, OXALIC acid

مستخلص: The purpose of this study was to prepare mackerel peptides (MPs) with calcium-binding capacity through an enzyme method and to investigate the potential role they play in improving the bioavailability of calcium in vitro. The calcium-binding capacity, degree of hydrolysis (DH), molecular weight (MW), and charge distribution changes with the enzymolysis time of MPs were measured. The structural characterization of mackerel peptide–calcium (MP–calcium) complexes was performed using spectroscopy and morphology analysis. The results showed that the maximum calcium-binding capacity of the obtained MPs was 120.95 mg/g when alcalase was used for 3 h, with a DH of 15.45%. Moreover, with an increase in hydrolysis time, the MW of the MPs decreased, and the negative charge increased. The carboxyl and amino groups in aspartic (Asp) and glutamate (Glu) of the MPs may act as calcium-binding sites, which are further assembled into compact nanoscale spherical complexes with calcium ions through intermolecular interactions. Furthermore, even under the influence of oxalic acid, MP–calcium complexes maintained a certain solubility. This study provides a basis for developing new calcium supplements and efficiently utilizing the mackerel protein resource. [ABSTRACT FROM AUTHOR]

: Copyright of Foods is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Lin, Yiting, Li, Daichao, Peng, Peng, Liang, Jianqin, Ding, Fei, Jin, Xinlei, Zeng, Zhan

المصدر: Transactions in GIS; Jun2024, Vol. 28 Issue 4, p902-924, 23p

مصطلحات موضوعية: KNOWLEDGE graphs, CASE-based reasoning, CROWDSENSING

مصطلحات جغرافية: CHINA

مستخلص: The lack of multidimensional knowledge means that current reasoning methods for rice fertilization cannot make decisions accurate when faced with complex spatiotemporal conditions in general. Therefore, we propose a reasoning method for rice fertilization strategy based on spatiotemporal knowledge graph. First, we systematically organize multisource expert knowledge about rice fertilization, and construct an ontology for rice fertilization consisting of five core elements: rice variety, planting environment, nutrition diagnosis, fertilization schemes, and time and place. Spatiotemporal differences in rice fertilization knowledge are expressed by assessing spatiotemporal concepts, relations, and state instances. Second, we propose a reasoning method for rice fertilization strategy based on the constructed knowledge graph. This method leverages a certainty factor model for nutrition diagnosis and integrates case‐based and rule‐based reasoning to determine fertilization schemes for different stages. Finally, taking Pucheng County, China, as an example, knowledge from crowd‐sensing data is obtained to construct a knowledge graph using the proposed method. The results demonstrate the method can support the expression and complex reasoning of rice fertilization decisions under different spatiotemporal conditions. [ABSTRACT FROM AUTHOR]

: Copyright of Transactions in GIS is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Li, Ling, Wang, Tao, Chen, Zhi, Liang, Jianqin, Ding, Hong

المصدر: Sci Rep ; ISSN:2045-2322 ; Volume:14 ; Issue:1

مصطلحات موضوعية: Immune microenvironment, Neural network model, PTB, Single-cell, Subtypes, Tuberculosis

الوصف: Tuberculosis (TB) remains a significant global health threat, necessitating effective strategies for diagnosis, prognosis, and treatment. This study employs a multi-cohort analysis approach to unravel the immune microenvironment of TB and delineate distinct subtypes within pulmonary TB (PTB) patients. Leveraging functional gene expression signatures (Fges), we identified three PTB subtypes (C1, C2, and C3) characterized by differential immune-inflammatory activity. These subtypes exhibited unique molecular features, functional disparities, and cell infiltration patterns, suggesting varying disease trajectories and treatment responses. A neural network model was developed to predict PTB progression based on a set of biomarker genes, achieving promising accuracy. Notably, despite both genders being affected by PTB, females exhibited a relatively higher risk of deterioration. Additionally, single-cell analysis provided insights into enhanced major histocompatibility complex (MHC) signaling in the rapid clearance of early pathogens in the C3 subgroup. This comprehensive approach offers valuable insights into PTB pathogenesis, facilitating personalized treatment strategies and precision medicine interventions.

العلاقة: https://doi.org/10.1038/s41598-024-63365-5Test; https://pubmed.ncbi.nlm.nih.gov/38858405Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164950Test/

الإتاحة: https://doi.org/10.1038/s41598-024-63365-5Test
https://pubmed.ncbi.nlm.nih.gov/38858405Test
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164950Test/

المؤلفون: Xu, Fudong, Du, Weili, Li, Chengjun, Li, Ye, Li, Zhihui, Han, Wenge, Li, Huimin, Liang, Jianqin, Zhao, Dongmei, Yang, Xinting, Wang, Feng, Long, Chaolian, Xing, Xuya, Tan, Jing, Zhang, Nana, Sun, Zuyu, Che, Nanying

المصدر: Clin Microbiol Infect ; ISSN:1469-0691

مصطلحات موضوعية: Cell-free DNA, Diagnosis, Droplet digital PCR, Pleural effusion, Tuberculous pleurisy

الوصف: Tuberculous pleurisy is one of the most common types of extra-pulmonary tuberculosis, but the sensitivity of conventional mycobacterial culture (Culture) or Xpert MTB/RIF assay (Xpert) is not satisfying. This multicentre cohort study evaluated the accuracy of a new cell-free DNA droplet digital PCR assay (cf-ddPCR) for diagnosing tuberculous pleurisy.

العلاقة: https://doi.org/10.1016/j.cmi.2024.05.012Test; https://pubmed.ncbi.nlm.nih.gov/38810928Test

الإتاحة: https://doi.org/10.1016/j.cmi.2024.05.012Test
https://pubmed.ncbi.nlm.nih.gov/38810928Test

المؤلفون: Yan, Jiongyi, Feng, Yinyi, Fang, Xuewan, Cui, Xiaojuan, Xia, Xing, Li, Fang, Luo, Weisheng, Liang, Jianqin, Feng, Jianfang, Yu, Kai

المساهمون: National Natural Science Foundation of China, Innovation-Driven Development Project of Guangxi, Graduate Education Innovation Program of Guangxi University of Chinese Medicine, Guangxi First-class Discipline: Chinese Materia Medica

المصدر: Pharmaceutical Biology ; volume 60, issue 1, page 1264-1277 ; ISSN 1388-0209 1744-5116

الإتاحة: https://doi.org/10.1080/13880209.2022.2086584Test