مستخلص: A pleuromutilin cinnamic acid ester compound with activities against antibiotic-resistant infections having the following formula (I) [chemical expression included] R1, R2, R3, R4 and R5 are independently hydrogen, halogen, nitro, cyano, methoxy, trifluoromethyl, benzyloxy, aryl or dimethylamino.

مستخلص: A Syringa microphylla seed extract includes the following components by weight based on a total weight of the Syringa microphylla seed extract: 0.4944-0.7142 mg/g of echinacoside, 6.624-7.617 mg/g of oleuropein, 0.4276-0.6309 mg/g of verbascoside, 3.927-4.684 mg/g of syringin, and 4.505-5.250 mg/g of forsythiaside B. A method of preparing the Syringa microphylla seed extract is disclosed. A composition for treating antibiotic-resistant infections that includes the Syringa microphylla seed extract is also disclosed.

مستخلص: A method of preparing a hydroxytyrosol sturgeon skin gelatin film with antioxidant activity and Salmonella detection ability includes: (1) mixing a neutral red indicator solution and distilled water at room temperature to obtain a mixture solution, and adding 1 mol/L NaHCO3 solution to the mixture solution until discoloration to obtain an alkaline solution; (2) adding an amount of the alkaline solution obtained in step (1) to a mixture of Sturgeon skin gelatin and distilled water to obtain an alkaline gelatin solution; (3) adding hydroxytyrosol and glycerin and propylene glycol to the alkaline gelatin solution to obtain a hydroxytyrosol gelatin solution, and heating the hydroxytyrosol gelatin solution at 40-70° C.; (4) ultrasonicating the hydroxytyrosol gelatin solution from step (3), placing the hydroxytyrol gelatin solution evenly on a clean plexiglass plate, drying the hydroxytyrol gelatin solution on an ultra-clean worktable to obtain the hydroxytyrosol sturgeon skin gelatin film.

المؤلفون: Liu, Aimei, Liu, Sang, Lv, Kangyan, Zhu, Qingdong, Wen, Jun, Li, Jianpeng, Liang, Chengyuan, Huang, Xuegang, Gong, Chunming, Sun, Qingfeng, Gu, Hongcang

المصدر: Frontiers in Microbiology ; volume 15 ; ISSN 1664-302X

مصطلحات موضوعية: Microbiology (medical), Microbiology

الوصف: Background Resistance to anti-tuberculous drugs is a major challenge in the treatment of tuberculosis (TB). We aimed to evaluate the clinical availability of nanopore-based targeted next-generation sequencing (NanoTNGS) for the diagnosis of drug-resistant tuberculosis (DR-TB). Methods This study enrolled 253 patients with suspected DR-TB from six hospitals. The diagnostic efficacy of NanoTNGS for detecting Mycobacterium tuberculosis and its susceptibility or resistance to first- and second-line anti-tuberculosis drugs was assessed by comparing conventional phenotypic drug susceptibility testing (pDST) and Xpert MTB/RIF assays. NanoTNGS can be performed within 12 hours from DNA extraction to the result delivery. Results NanoTNGS showed a remarkable concordance rate of 99.44% (179/180) with the culture assay for identifying the Mycobacterium tuberculosis complex. The sensitivity of NanoTNGS for detecting drug resistance was 93.53% for rifampicin, 89.72% for isoniazid, 85.45% for ethambutol, 74.00% for streptomycin, and 88.89% for fluoroquinolones. Specificities ranged from 83.33% to 100% for all drugs tested. Sensitivity for rifampicin-resistant tuberculosis using NanoTNGS increased by 9.73% compared to Xpert MTB/RIF. The most common mutations were S531L (codon in E. coli) in the rpoB gene, S315T in the katG gene, and M306V in the embB gene, conferring resistance to rifampicin, isoniazid, and ethambutol, respectively. In addition, mutations in the pncA gene, potentially contributing to pyrazinamide resistance, were detected in 32 patients. Other prevalent variants, including D94G in the gyrA gene and K43R in the rpsL gene, conferred resistance to fluoroquinolones and streptomycin, respectively. Furthermore, the rv0678 R94Q mutation was detected in one sample, indicating potential resistance to bedaquiline. Conclusion NanoTNGS rapidly and accurately identifies resistance or susceptibility to anti-TB drugs, outperforming traditional methods. Clinical implementation of the technique can recognize DR-TB in time and ...

الإتاحة: https://doi.org/10.3389/fmicb.2024.1349715Test

مستخلص: A compound with anti-drug resistant bacteria activity having the following formula (I): [chemical expression included] is disclosed. A method of preparing the compound of formula (I) is also disclosed.

مستخلص: A compound with anti-drug resistant bacteria activity having the following formula (I): [chemical expression included] is disclosed. The methods of preparing the compound of formula (I) are also disclosed.

مستخلص: A compound with anti-drug resistant bacteria activity having the following formula (I): [chemical expression included] is disclosed. The methods of preparing the compound of formula (I) are also disclosed.

مستخلص: A compound with anti-drug resistant bacteria activity having the formula (I): [chemical expression included] (I) is disclosed. The methods of preparing the compound of formula (I) are also disclosed.

مستخلص: A compound with anti-drug resistant bacteria activity having the following formula (I): [chemical expression included] is disclosed. The methods of preparing the compound of formula (I) are also disclosed.

مستخلص: A method of preparing a Lilac Ginkgo Brick Tea includes the following steps: (1) mixing and drying fresh Lilac leaves and Ginkgo leaves to obtain a Lilac Ginkgo Tea; (2) fermenting the Lilac Ginkgo Tea to obtain a Fermented Lilac Ginkgo Tea; (3) mixing the Fermented Lilac Ginkgo Tea with a black tea to obtain a Fermented Lilac Ginkgo Tea Mixture; and (4) processing the Fermented Lilac Ginkgo Tea Mixture to obtain the Lilac Ginkgo Brick Tea.