المؤلفون: Li, Shu-Ying

المساهمون: Arbeitsgruppe Zellkulturtechnik, Gesellschaft fiir Biotechnologische Forschung, Mascheroder Weg 1, D-3300 Braunschweig, FRG.

الوصف: Efficient recombinant protein production processes should optimize both productivity and yield. The fermentation process should also preserve the integrity and quality of the desired protein product. AT III (antithrombin III) is one of the most important serine protease inhibitors in plasma. It is an @,-glycoprotein of M, 60,000 to 64,000 containing three or four polysaccharide chains attached by N-glycosidic linkage to asparagine residues. The biological activity of AT III can be enhanced 2-3 orders of magnitude by binding with the cofactor heparin (1). Affinity chromatography of plasma-derived AT III on immobilized heparin resulted in two fractions of AT II which differ from each other in their affinity to heparin; their difference in affinity to heparin is due to different degrees of glycosylation (2). Concerns have been raised that if BHK-derived AT III exhibit the same behavior on purification as its plasma-derived counterpart. In the fermentation of AT III producing BHK (baby hamster kidney) cells, productivity was monitored and the possible influence of serum-free cultivation conditions on protein quality in termsof both integrity (against proteolytic activity) and affinity to heparin was investigated.

العلاقة: GBF monographs; Volume 15; Protein glycosylation, 283 - 286; http://hdl.handle.net/10033/623686Test; Protein glycosylation - cellular, biotechnological and analytical aspects, 1991

الإتاحة: http://hdl.handle.net/10033/623686Test

المؤلفون: Liu, Jia-Meng, Liang, Yan-Tian, Wang, Shan-Shan, Jin, Nuo, Sun, Jing, Lu, Cong, Sun, Yu-Feng, Li, Shu-Ying, Fan, Bei, Wang, Feng-Zhong

المصدر: Scientific Reports ; volume 13, issue 1 ; ISSN 2045-2322

مصطلحات موضوعية: Multidisciplinary

الوصف: The growth of endophytic bacteria is influenced by the host plants and their secondary metabolites and activities. In this study, P. megaterium P-NA14 and P. megaterium D-HT207 were isolated from potato tuber and dendrobium stem respectively. They were both identified as Priestia megaterium . The antimicrobial activities and metabolites of both strains were explored. For antimicrobial activities, results showed that P. megaterium P-NA14 exhibited a stronger inhibition effect on the pathogen of dendrobium, while P. megaterium D-HT207 exhibited a stronger inhibition effect on the pathogen of potato. The supernatant of P. megaterium P-NA14 showed an inhibition effect only on Staphylococcus aureus , while the sediment of P. megaterium D-HT207 showed an inhibition effect only on Escherichia coli . For metabolomic analysis, the content of L -phenylalanine in P. megaterium P-NA14 was higher than that of P. megaterium D-HT207, and several key downstream metabolites of L -phenylalanine were associated with inhibition of S. aureus including tyrosine, capsaicin, etc. Therefore, we speculated that the different antimicrobial activities between P. megaterium P-NA14 and P. megaterium D-HT207 were possibly related to the content of L -phenylalanine and its metabolites. This study preliminarily explored why the same strains isolated from different hosts exhibit different activities from the perspective of metabolomics.

الإتاحة: https://doi.org/10.1038/s41598-023-32337-6Test
https://www.nature.com/articles/s41598-023-32337-6.pdfTest
https://www.nature.com/articles/s41598-023-32337-6Test

المؤلفون: Cai, Hong, Zhao, Yan-Jie, He, Fan, Li, Shu-Ying, Li, Zong-Lei, Zhang, Wu-Yang, Zhang, Yao, Cheung, Teris, Ng, Chee H., Sha, Sha, Xiang, Yu-Tao

المساهمون: National Natural Science Foundation of China, Universidade de Macau

المصدر: Translational Psychiatry ; volume 13, issue 1 ; ISSN 2158-3188

مصطلحات موضوعية: Biological Psychiatry, Cellular and Molecular Neuroscience, Psychiatry and Mental health

الوصف: To assess the inter-relationships between residual depressive symptoms (RDS) and Internet addiction (IA) using network analysis among clinically stable adolescents with major psychiatric disorders during the COVID-19 pandemic. RDS and IA were assessed using the Patient Health Questionnaire-9 (PHQ-9) and the Internet Addiction Test (IAT), respectively. Central symptoms and bridge symptoms in the network model were examined. A total of 1,454 adolescents met the study criteria and were included in the analyses. The prevalence of IA was 31.2% (95% CI: 28.8%-33.6%). In the network analysis, the nodes IAT15 (“Preoccupation with the Internet”), PHQ2 (“Sad mood”), and PHQ1 (“Anhedonia”) were the most central symptoms in the IA-RDS network model. Bridge symptoms included IAT10 (“Sooth disturbing about your Internet use”), PHQ9 (“Suicide ideation”), and IAT3 (“Prefer the excitement online to the time with others”). Additionally, PHQ2 (“Sad mood”) was the main node linking “Anhedonia” to other IA clusters. Internet addiction was common among clinically stable adolescents with major psychiatric disorders during the COVID-19 pandemic. Core and bridge symptoms identified in this study could be prioritized as targets for the prevention and treatment of IA in this population.

الإتاحة: https://doi.org/10.1038/s41398-023-02468-5Test
https://www.nature.com/articles/s41398-023-02468-5.pdfTest
https://www.nature.com/articles/s41398-023-02468-5Test

المؤلفون: Li, Shu-Ying, Chen, Ting, Chen, Qi, Wang, Dong, Zhu, Guangshan

المساهمون: Natural Science Foundation of Jilin Province, National Natural Science Foundation of China, Education Department of Jilin Province, Fundamental Research Funds for the Central Universities

المصدر: Chemical Science ; volume 14, issue 10, page 2646-2651 ; ISSN 2041-6520 2041-6539

الوصف: A lower molecular concentration in solution facilitates more efficient amplification of chirality at the surface, which is successively reproduced by a cooperative 2D equilibrium model derived from Langmuir-type adsorption.

الإتاحة: https://doi.org/10.1039/d2sc06746cTest
http://pubs.rsc.org/en/content/articlepdf/2023/SC/D2SC06746CTest

المؤلفون: Liu, Jun-Ying, Zhang, Jian-Feng, Wu, Han-Lian, Chen, Zhen, Li, Shu-Ying, Li, Hong-Mei, Zhang, Cui-Ping, Zhou, Yuan-Qing, Lu, Can-Hua

المصدر: Frontiers in Microbiology ; volume 14 ; ISSN 1664-302X

الوصف: A Gram-negative, aerobic, rod-shaped, motile bacterium with multi-flagella, strain RS T , was isolated from bacterial wilt of tobacco in Yuxi city of Yunnan province, China. The strain contains the major fatty acids of C 16:0 , summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ), and summed feature 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c ). The polar lipid profile of strain RS T consists of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and unidentified aminophospholipid. Strain RS T contains ubiquinones Q-7 and Q-8. 16S rRNA gene sequence (1,407 bp) analysis showed that strain RS T is closely related to members of the genus Ralstonia and shares the highest sequence identities with R. pseudosolanacearum LMG 9673 T (99.50%), R. syzygii subsp. indonesiensis LMG 27703 T (99.50%), R. solanacearum LMG 2299 T (99.28%), and R. syzygii subsp. celebesensis LMG 27706 T (99.21%). The 16S rRNA gene sequence identities between strain RS T and other members of the genus Ralstonia were below 98.00%. Genome sequencing yielded a genome size of 5.61 Mbp and a G + C content of 67.1 mol%. The genomic comparison showed average nucleotide identity (ANIb) values between strain RS T and R. pseudosolanacearum LMG 9673 T , R. solanacearum LMG 2299 T , and R. syzygii subsp. indonesiensis UQRS 627 T of 95.23, 89.43, and 91.41%, respectively, and the corresponding digital DNA–DNA hybridization (dDDH) values (yielded by formula 2) were 66.20, 44.80, and 47.50%, respectively. In addition, strains belonging to R. solanacearum phylotype I shared both ANIb and dDDH with strain RS T above the species cut-off values of 96 and 70%, respectively. The ANIb and dDDH values between the genome sequences from 12 strains of R. solanacearum phylotype III (Current R. pseudosolanacearum ) and those of strain RS T were below the species cut-off values. Based on these data, we concluded that strains of phylotype I, including RS T , represent a novel species of the genus Ralstonia , for which the name Ralstonia nicotianae sp. nov. is ...

الإتاحة: https://doi.org/10.3389/fmicb.2023.1135872Test

المؤلفون: Xie, Xiao-Meng, Cai, Hong, Li, Shu-Ying, Li, Zong-Lei, Zhang, Wu-Yang, Zhao, Yan-Jie, Zhang, Yao, Ungvari, Gabor S., Tang, Yi-Lang, He, Fan, Xiang, Yu-Tao

المساهمون: National Natural Science Foundation of China, University of Macau

المصدر: Frontiers in Psychology ; volume 13 ; ISSN 1664-1078

مصطلحات موضوعية: General Psychology

الوصف: Objective This study examined the prevalence of cyberbullying and its relationship with residual depressive symptoms in this patient population during the COVID-19 outbreak using network analysis. Methods This was a multicenter, cross-sectional study. Adolescent patients attending maintenance treatment at outpatient departments of three major psychiatric hospitals were included. Experience of cyberbullying was measured with a standard question, while the severity of Internet addiction and depressive symptoms were measured using the Internet Addiction Test and the Patient Health Questionnaire-9, respectively. The network structure of depression and cyberbully were characterized and indices of “Expected Influence” was used to identify symptoms central to the network. To identify particular symptoms that were directly associated with cyberbully, the flow function was used. Results Altogether 1,265 patients completed the assessments. The overall prevalence of cyberbullying was 92.3% (95% confidence interval (CI): 90.8–93.7%). Multiple logistic regression analysis revealed that male gender ( p = 0.04, OR = 1.72, 95%CI: 1.04–2.85) was significantly associated with higher risk of cyberbullying, while a relapse of illness during the COVID-19 pandemic was significantly associated with a lower risk of cyberbullying ( p = 0.03, OR = 0.50, 95%CI: 0.27–0.93). In the network of depression and cyberbully, “Sad mood,” “Anhedonia” and “Energy” were the most central (influential) symptoms. Furthermore, “Suicidal ideation” had the strongest negative association with cyberbully followed by “Guilt”. Conclusion During the COVID-19 pandemic, the experience of cyberbullying was highly prevalent among clinically stable adolescent psychiatric patients, particularly male patients. This finding should raise awareness of this issue emphasizing the need for regular screening and interventions for adolescent patients. Central symptoms (e.g., “Sad mood,” “Anhedonia” and “Energy”) identified in this study should be targeted in interventions and ...

الإتاحة: https://doi.org/10.3389/fpsyg.2022.1080192Test

المؤلفون: Gu, Zhen‐Yi, Cao, Jun‐Ming, Li, Kai, Guo, Jin‐Zhi, Wang, Xiao‐Tong, Zheng, Shuo‐Hang, Zhao, Xin‐Xin, Li, Bao, Li, Shu‐Ying, Li, Wen‐Liang, Wu, Xing‐Long

المصدر: Angewandte Chemie; 7/22/2024, Vol. 136 Issue 30, p1-11, 11p

مستخلص: 2D compounds exfoliated from weakly bonded bulk materials with van der Waals (vdW) interaction are easily accessible. However, the strong internal ionic/covalent bonding of most inorganic crystal frameworks greatly hinders 2D material exfoliation. Herein, we first proposed a radical/strain‐synergistic strategy to exfoliate non‐vdW interacting pseudo‐layered phosphate framework. Specifically, hydroxyl radicals (⋅OH) distort the covalent bond irreversibly, meanwhile, H2O molecules as solvents, further accelerating interlayered ionic bond breakage but mechanical expansion. The innovative 2D laminar NASICON‐type Na3V2(PO4)2O2F crystal, exfoliated by ⋅OH/H2O synergistic strategy, exhibits enhanced sodium‐ion storage capacity, high‐rate performance (85.7 mAh g−1 at 20 C), cyclic life (2300 cycles), and ion migration rates, compared with the bulk framework. Importantly, this chemical/physical dual driving technique realized the effective exfoliation for strongly coupled pseudo‐layered frameworks, which accelerates 2D functional material development. [ABSTRACT FROM AUTHOR]

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المؤلفون: Li, Shu-Ying¹ (AUTHOR), Yin, Qi-Min¹ (AUTHOR), Gu, Zhen-Yi^1,2 (AUTHOR) guzy166@nenu.edu.cn, Liu, Yan¹ (AUTHOR), Liu, Yan-Ning¹ (AUTHOR), Su, Meng-Yuan¹ (AUTHOR), Wu, Xing-Long^1,2 (AUTHOR) xinglong@nenu.edu.cn

المصدر: Journal of Colloid & Interface Science. Jun2024, Vol. 664, p381-388. 8p.

مصطلحات موضوعية: *SODIUM ions, *CATHODES, *JAHN-Teller effect, *STRUCTURAL dynamics, *ION transport (Biology), *ELECTRIC batteries

مستخلص: In this study, the electrochemical performance of Na 3 MnTi(PO 4) 3 material has been improved through cation/anion-dual regulation. This dual regulation provides a strategy for phosphate materials as an advanced cathode for sodium-ion batteries. [Display omitted] Na 3 MnTi(PO 4) 3 (NMTP) emerges as a promising cathode material with high-performance for sodium-ion batteries (SIBs). Nevertheless, its development has been limited by several challenges, including poor electronic conductivity, the Mn3+ Jahn-Teller effect, and the presence of a Na+/Mn2+ cation mixture. To address these issues, we have developed a cation/anion-dual regulation strategy to activate the redox reactions involving manganese, thereby significantly enhancing the performance of NMTP. This strategy simultaneously enhances the structural dynamics and facilitates rapid ion transport at high rates by inducing the formation of sodium vacancy. The combined effects of these modifications lead to a substantial improvement in specific capacity (79.1 mAh/g), outstanding high-rate capabilities (35.9 mAh/g at 10C), and an ultralong cycle life (only 0.040 % capacity attenuation per cycle over 250 cycles at 1C for Na 3.34 Mn 1.2 Ti 0.8 (PO 3.98 F 0.02) 3) when used as a cathode material in SIBs. Furthermore, its performance in full cell demonstrates impressive rate capability (44.4 mAh/g at 5C) and exceptional cycling stability (with only 0.116 % capacity decay per cycle after 150 cycles at 1C), suggesting its potential for practical applications. This work presents a dual regulation strategy targeting different sites, offering a significant advancement in the development of NASICON phosphate cathodes for SIBs. [ABSTRACT FROM AUTHOR]

المؤلفون: Zhu, Yan, Li, Shu-Ying, Zhang, Lu-Jia, Lei, Bo, Wang, Yong-Chen, Wang, Zhongfeng

المصدر: NeuroReport ; ISSN 0959-4965

الوصف: The aim of this study was to explore the neuroprotective effects of the P2X7 receptor antagonist A740003 on retinal ganglion cells (RGCs) in chronic intraocular hypertension (COH) experimental glaucoma mouse model. Bioinformatics was used to analyze the glaucoma-related genes. Western blot, real-time fluorescence quantitative PCR, and immunofluorescence staining techniques were employed to explore the mechanisms underlying the neuroprotective effects of A740003 on RGCs in COH retinas. Bioinformatic analysis revealed that oxidative stress, neuroinflammation, and cell apoptosis were highly related to the pathogenesis of glaucoma. In COH retinas, intraocular pressure elevation significantly increased the levels of translocator protein, a marker of microglial activation, which could be reversed by intravitreal preinjection of A740003. A740003 also suppressed the increased mRNA levels of proinflammatory cytokines interleukin (IL) 1β and tumor necrosis factor α in COH retinas. In addition, although the mRNA levels of anti-inflammatory cytokine IL-4 and IL-10 were kept unchanged in COH retinas, administration of A740003 could increase their levels. The mRNA and protein levels of Bax and cleaved caspase-3 were increased in COH retinas, which could be partially reversed by A740003, while the levels of Bcl-2 kept unchanged in COH retinas with or without the injections of A740003. Furthermore, A740003 partially attenuated the reduction in the numbers of Brn-3a-positive RGCs in COH mice. A740003 could provide neuroprotective roles on RGCs by inhibiting the microglia activation, attenuating the retinal inflammatory response, reducing the apoptosis of RGCs, and enhancing the survival of RGCs in COH experimental glaucoma.

الإتاحة: https://doi.org/10.1097/wnr.0000000000002071Test

المؤلفون: Li, Shu-Ying, Chen, Rui, Tang, Wei-Ting, Ma, Xiao-Jie, Cai, Hua-Bin, You, Yong

المصدر: Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; ISSN:1000-503X

مصطلحات موضوعية: anxiety, cerebrovascular reactivity, depression, end-stage renal disease, transcranial Doppler sonography

الوصف: Objective To investigate the relationship between cerebrovascular reactivity (CVR) and emotional disorders in the patients undergoing continuous hemodialysis for end-stage renal disease (ESRD).Methods The clinical data of the ESRD patients undergoing continuous hemodialysis were collected.Anxiety and depression of the patients were assessed by the Hamilton anxiety scale (HAMA) and Beck depression inventory,respectively.The cerebral hemodynamic changes during the breath holding test were monitored by transcranial Doppler sonography,and the breath-holding index (BHI) was calculated.The BHI≥0.69 and BHI<0.69 indicate normal CVR and abnormal CVR,respectively.Binary Logistic regression was employed to analyze the factors affecting the depressive state of ESRD patients.Results The group with abnormal CVR exhibited higher total cholesterol level (P=0.010),low density lipoprotein level (P=0.006),and incidence of depression (P=0.012) than the group with normal CVR.Compared with the non-depression group,the depression group displayed prolonged disease course (P=0.039),reduced body mass index (P=0.048),elevated HAMA score (P=0.001),increased incidence of anxiety (P<0.001),decreased BHI (P=0.015),and increased incidence of abnormal CVR (P=0.012).Binary Logistic regression analysis indicated anxiety as a contributing factor (OR=22.915,95%CI=2.653-197.956,P=0.004) and abnormal CVR as a risk factor (OR=0.074,95%CI=0.008-0.730,P=0.026) for depression.Conclusion Impaired CVR could pose a risk for depression in the patients with ESRD.

العلاقة: https://doi.org/10.3881/j.issn.1000-503X.15891Test; https://pubmed.ncbi.nlm.nih.gov/38864299Test

الإتاحة: https://doi.org/10.3881/j.issn.1000-503X.15891Test
https://pubmed.ncbi.nlm.nih.gov/38864299Test