المؤلفون: Yang, Xingdong, Li, Qingmei, Kwee, Sharon, Yang, Jifei, Zhang, Qianqian, Hu, Xiaofei

المساهمون: Khamesipour, Faham, National Science & Technology Pillar Program of the 12th Five-Year Plan, Agence Thématique de Recherche en Science et Technologie

المصدر: PLOS ONE ; volume 19, issue 3, page e0299709 ; ISSN 1932-6203

الوصف: Marbofloxacin (MBF) was once widely used as a veterinary drug to control diseases in animals. MBF residues in animal food endanger human health. In the present study, an immunochromatographic strip assay (ICSA) utilizing a competitive principle was developed to rapidly detect MBF in beef samples. The 50% inhibitory concentration (IC 50 ) and the limit of detection (LOD) of the ICSAs were 2.5 ng/mL and 0.5 ng/mL, respectively. The cross-reactivity (CR) of the MBF ICSAs to Ofloxacin (OFL), enrofloxacin (ENR), norfloxacin (NOR), and Ciprofloxacin (CIP) were 60.98%, 32.05%, 22.94%, and 23.58%, respectively. The CR for difloxacin (DIF) and sarafloxacin (SAR) was less than 0.1%. The recovery rates of MBF in spiked beef samples ranged from 82.0% to 90.4%. The intra-assay and interassay coefficients of variation (CVs) were below 10%. In addition, when the same authentic beef samples were detected in a side-by-side comparison between the ICSAs and HPLC‒MS, no statistically significant difference was observed. Therefore, the proposed ICSAs can be a useful tool for monitoring MBF residues in beef samples in a qualitative and quantitative manner.

الإتاحة: https://doi.org/10.1371/journal.pone.0299709Test

المؤلفون: Xu, Qianru, Ma, Fanshu, Yang, Daichang, Li, Qingmei, Yan, Liming, Ou, Jiquan, Zhang, Longxian, Liu, Yunchao, Zhan, Quan, Li, Rui, Wei, Qiang, Hu, Hui, Wang, Yanan, Li, Xueyang, Zhang, Shenli, Yang, Jifei, Chai, Shujun, Du, Yongkun, Wang, Li, Zhang, Erqin, Zhang, Gaiping

المصدر: Plant Biotechnology Journal ; volume 21, issue 12, page 2546-2559 ; ISSN 1467-7644 1467-7652

الوصف: Summary Pestiviruses, including classical swine fever virus, remain a concern for global animal health and are responsible for major economic losses of livestock worldwide. Despite high levels of vaccination, currently available commercial vaccines are limited by safety concerns, moderate efficacy, and required high doses. The development of new vaccines is therefore essential. Vaccine efforts should focus on optimizing antigen presentation to enhance immune responses. Here, we describe a simple herringbone‐dimer strategy for efficient vaccine design, using the classical swine fever virus E2 expressed in a rice endosperm as an example. The expression of rE2 protein was identified, with the rE2 antigen accumulating to 480 mg/kg. Immunological assays in mice, rabbits, and pigs showed high antigenicity of rE2. Two immunizations with 284 ng of the rE2 vaccine or one shot with 5.12 μg provided effective protection in pigs without interference from pre‐existing antibodies. Crystal structure and small‐angle X‐ray scattering results confirmed the stable herringbone dimeric conformation, which had two fully exposed duplex receptor binding domains. Our results demonstrated that rice endosperm is a promising platform for precise vaccine design, and this strategy can be universally applied to other Flaviviridae virus vaccines.

الإتاحة: https://doi.org/10.1111/pbi.14152Test

المؤلفون: Li, Qingmei, Yang, Jiancheng, Tang, Qinghua, Feng, Yan, Pan, Mingming, Che, Maohong, Shi, Jie, Zeng, Yuhong

المساهمون: Xi’an Science and Technology Program Project, Scientific Research Program of Shaanxi Province Health Commission, Key Research and Development Projects of Shaanxi Province

المصدر: BMC Geriatrics ; volume 23, issue 1 ; ISSN 1471-2318

مصطلحات موضوعية: Geriatrics and Gerontology

الوصف: Background There is a gender difference in the acceptance of osteoporosis diagnosis and treatment in patients after fragility fractures, but this difference is rarely assessed during hospitalization, and it is unclear whether these differences are age-dependent. This study aimed to evaluate the differences between male and female fragility fracture patients of different age groups who received the diagnosis and treatment of osteoporosis during hospitalization. Methods 31,265 fragility fracture patients aged ≥ 50 years from the Fragility Fracture Management Database in a high-volume orthopedic hospital from December 2019 to February 2023 were included in this study. We compared the differences in the rates of men and women with fragility fracture who received the measurement of bone mineral density (BMD) and bone metabolism biochemical markers (BMBMs) and treatment with anti-osteoporosis medications (AOMs), and follow-up to the internal medicine clinic within 3 months after discharge, across all age groups and across different age stages (50–59, 60–69, 70–79, and ≥ 80 years). Results The detection rates of female patients receiving BMD and BMBMs during hospitalization were 31.88% and 5.30%, respectively, compared with 22.23% and 2.69% for men. The rate of receiving any AOMs treatment was 44.63% for women and 31.60% for men. The follow-up rate of returning to the internal medicine clinic within 3 months after discharge was 9.79% for women compared to 3.00% for men. There was a significant difference between males compared to females ( P < 0.0001). Analysis of patients by different age group revealed that differences in the diagnosis and treatment of osteoporosis were found only in patients under 80 years of age, while gender differences in the return to the internal medicine clinic for follow-up after discharge were present in all age groups. Conclusions Gender differences present in osteoporosis management in patients with fragility fracture during hospitalization, especially for patients under 80 ...

الإتاحة: https://doi.org/10.1186/s12877-023-04430-yTest

المؤلفون: Zhang, Hongxi, Wei, Taoshu, Li, Qingmei, Fu, Liang, He, Lisheng, Wang, Yong

المصدر: PeerJ ; volume 11, page e16257 ; ISSN 2167-8359

الوصف: Our knowledge on biogeochemistry and microbial ecology of marine blue holes is limited due to challenges in collecting multilayered water column and oxycline zones. In this study, we collected samples from 16 water layers in Yongle blue hole (YBH) located in the South China Sea using the in situ microbial filtration and fixation (ISMIFF) apparatus. The microbial communities based on 16S rRNA metagenomic reads for the ISMIFF samples showed high microbial diversity and consistency among samples with similar dissolved oxygen levels. At the same depth of the anoxic layer, the ISMIFF samples were dominated by sulfate-reducing bacteria from Desulfatiglandales (17.96%). The sulfide concentration is the most significant factor that drives the division of microbial communities in YBH, which might support the prevalence of sulfate-reducing microorganisms in the anoxic layers. Our results are different from the microbial community structures of a Niskin sample of this study and the reported samples collected in 2017, in which a high relative abundance of Alteromonadales (26.59%) and Thiomicrospirales (38.13%), and Arcobacteraceae (11.74%) was identified. We therefore demonstrate a new profile of microbial communities in YBH probably due to the effect of sampling and molecular biological methods, which provides new possibilities for further understanding of the material circulation mechanism of blue holes and expanding anoxic marine water zones under global warming.

الإتاحة: https://doi.org/10.7717/peerj.16257Test
https://peerj.com/articles/16257.pdfTest
https://peerj.com/articles/16257.xmlTest
https://peerj.com/articles/16257.htmlTest

المؤلفون: Huang, Dageng, Wang, Jihan, Zeng, Yuhong, Li, Qingmei, Wang, Yangyang

المساهمون: National Natural Science Foundation of China, Fundamental Research Funds for the Central Universities

المصدر: Frontiers in Microbiology ; volume 14 ; ISSN 1664-302X

الوصف: Osteoporosis (OP) is a metabolic bone disorder characterized by low bone mass and deterioration of micro-architectural bone tissue. The most common type of OP is postmenopausal osteoporosis (PMOP), with fragility fractures becoming a global burden for women. Recently, the gut microbiota has been connected to bone metabolism. The aim of this study was to characterize the gut microbiota signatures in PMOP patients and controls. Fecal samples from 21 PMOP patients and 37 controls were collected and analyzed using amplicon sequencing of the V3-V4 regions of the 16S rRNA gene. The bone mineral density (BMD) measurement and laboratory biochemical test were performed on all participants. Two feature selection algorithms, maximal information coefficient (MIC) and XGBoost, were employed to identify the PMOP-related microbial features. Results showed that the composition of gut microbiota changed in PMOP patients, and microbial abundances were more correlated with total hip BMD/ T -score than lumbar spine BMD/ T -score. Using the MIC and XGBoost methods, we identified a set of PMOP-related microbes; a logistic regression model revealed that two microbial markers (Fusobacteria and Lactobacillaceae ) had significant abilities in disease classification between the PMOP and control groups. Taken together, the findings of this study provide new insights into the etiology of OP/PMOP, as well as modulating gut microbiota as a therapeutic target in the diseases. We also highlight the application of feature selection approaches in biological data mining and data analysis, which may improve the research in medical and life sciences.

الإتاحة: https://doi.org/10.3389/fmicb.2023.1113174Test

المؤلفون: Wang, Yinbiao, Zhang, Rui, Yang, Fuyun, Yang, Lin, Li, Qingmei, Guo, Junqing, Liu, Xiao, Song, Jie, Zhang, Guofu, Li, Juan, An, Zhen, Alexis, Neil E., Jaspers, Ilona, Wu, Weidong

المساهمون: Henan Provincial Science and Technology Research Project, National Natural Science Foundation of China

المصدر: Ecotoxicology and Environmental Safety ; volume 259, page 115069 ; ISSN 0147-6513

الإتاحة: https://doi.org/10.1016/j.ecoenv.2023.115069Test
https://api.elsevier.com/content/article/PII:S0147651323005730?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0147651323005730?httpAccept=text/plainTest

المؤلفون: Zhang, Qiaoli, Yu, Guanliu, Ding, Xinli, Zhang, Kaini, Sun, Wenbo, Li, Qingmei, Yi, Yunpeng, Wang, Jianhua, Pang, Xuehui, Chen, Lei

المصدر: Int J Biol Macromol ; ISSN:1879-0003 ; Volume:274 ; Issue:Pt 1

مصطلحات موضوعية: Duck hepatitis a virus 3, Lateral flow assay, Novel duck reovirus, RPA-CRISPR Cas12a/Cas13a

الوصف: The mixed infection of duck hepatitis A virus 3 (DHAV-3) and novel duck reovirus (NDRV) has caused significant losses to the global duck farming industry. On-site point-of-care testing of viruses plays a crucial role in the early diagnosis, prevention, and disease control. Here, we proposed an RPA-CRISPR Cas12a/Cas13a one-pot strategy (DRCFS) for rapid and simultaneous detection of DHAV-3 and NDRV. This method integrated the reaction of RPA and CRISPR Cas12a/Cas13a in a single tube, eliminating the need to open the lid during the intermediate processes and thereby avoiding aerosol contamination. On this basis, we proposed a dual RPA-CRISPR strategy coupled with a lateral flow analysis platform (DRC-LFA). This circumvented the necessity for complex instruments, enabling direct visual interpretation of results, making the test more accessible and user-friendly. Our findings demonstrated that the DRCFS method could detect DHAV-3 and NDRV at concentrations as low as 100 copy/μL, while DRC-LFA achieved limit of 101 copies/μL within 35 min. Furthermore, when DRCFS, DRC-LFA, and qPCR were employed collectively for clinical samples analysis, all three methods yielded consistent results. The specificity, sensitivity, and user-friendly of these methods rendered them invaluable for on-site virus detection.

العلاقة: https://doi.org/10.1016/j.ijbiomac.2024.133246Test; https://pubmed.ncbi.nlm.nih.gov/38908633Test

الإتاحة: https://doi.org/10.1016/j.ijbiomac.2024.133246Test
https://pubmed.ncbi.nlm.nih.gov/38908633Test

المؤلفون: Li, Qingmei, Wen, Yibin, Zheng, Juepeng, Zhang, Yuxiang, Fu, Haohuan

المساهمون: National Natural Science Foundation of China, Jiangsu Innovation Capacity Building Program

المصدر: IEEE Transactions on Geoscience and Remote Sensing ; volume 62, page 1-15 ; ISSN 0196-2892 1558-0644

الإتاحة: https://doi.org/10.1109/tgrs.2024.3400959Test
http://xplorestaging.ieee.org/ielx7/36/10354519/10530296.pdf?arnumber=10530296Test

المؤلفون: Zhao, Mengya, Dai, Beiying, Li, Xiaodong, Zhang, Yixin, Qiao, Chun, Qin, Yaru, Li, Zhao, Li, Qingmei, Wang, Shuzhen, Yang, Yong, Chen, Yijun

المصدر: Elife ; ISSN:2050-084X ; Volume:12

مصطلحات موضوعية: E. coli, cancer biology, cell biology, human, mouse

الوصف: Philadelphia chromosome-positive (Ph+) leukemia is a fatal hematological malignancy. Although standard treatments with tyrosine kinase inhibitors (TKIs) have achieved remarkable success in prolonging patient survival, intolerance, relapse, and TKI resistance remain serious issues for patients with Ph+ leukemia. Here, we report a new leukemogenic process in which RAPSYN and BCR-ABL co-occur in Ph+ leukemia, and RAPSYN mediates the neddylation of BCR-ABL. Consequently, neddylated BCR-ABL enhances the stability by competing its c-CBL-mediated degradation. Furthermore, SRC phosphorylates RAPSYN to activate its NEDD8 E3 ligase activity, promoting BCR-ABL stabilization and disease progression. Moreover, in contrast to in vivo ineffectiveness of PROTAC-based degraders, depletion of RAPSYN expression, or its ligase activity decreased BCR-ABL stability and, in turn, inhibited tumor formation and growth. Collectively, these findings represent an alternative to tyrosine kinase activity for the oncoprotein and leukemogenic cells and generate a rationale of targeting RAPSYN-mediated BCR-ABL neddylation for the treatment of Ph+ leukemia.

العلاقة: https://doi.org/10.7554/eLife.88375Test; https://pubmed.ncbi.nlm.nih.gov/38865175Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11168747Test/

الإتاحة: https://doi.org/10.7554/eLife.88375Test
https://pubmed.ncbi.nlm.nih.gov/38865175Test
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11168747Test/

المؤلفون: Wang, Hang, Hu, Bobin, Liang, Hengkai, Wang, Rongming, Wei, Lu, Su, Tumei, Li, Qingmei, Yin, Qianbing, Feng, Yanfei, Su, Minghua, Jiang, Jianning

المصدر: Int J Gen Med ; ISSN:1178-7074 ; Volume:17

مصطلحات موضوعية: gene rearrangement, hepatitis B virus, hepatocellular carcinoma, integration, long-term antiviral therapy

الوصف: Few studies have reported the integrated characteristics of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) after long-term antiviral therapy. This study aimed to investigate the HBV integration features in HBV-HCC patients who had undergone long-term antiviral therapy, evaluate their impact on clinical indicators, and analyze the potential mechanisms involved.

العلاقة: https://doi.org/10.2147/IJGM.S462844Test; https://pubmed.ncbi.nlm.nih.gov/38859910Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164208Test/

الإتاحة: https://doi.org/10.2147/IJGM.S462844Test
https://pubmed.ncbi.nlm.nih.gov/38859910Test
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11164208Test/