المؤلفون: Rizzo, M. I., Tomao, L., Tedesco, S., Cajozzo, M., Esposito, M., De Stefanis, C., Ferranti, A. M., Mezzogori, D., Palmieri, A., Pozzato, G., Algeri, M., Locatelli, F., Leone, L., Zama, M.

المصدر: Scientific Reports ; volume 11, issue 1 ; ISSN 2045-2322

مصطلحات موضوعية: Multidisciplinary

الوصف: Cleft lip and palate (CL/P) is the most prevalent craniofacial birth defect in humans. None of the surgical procedures currently used for CL/P repair lead to definitive correction of hard palate bone interruption. Advances in tissue engineering and regenerative medicine aim to develop new strategies to restore palatal bone interruption by using tissue or organ-decellularized bioscaffolds seeded with host cells. Aim of this study was to set up a new natural scaffold deriving from a decellularized porcine mucoperiosteum, engineered by an innovative micro-perforation procedure based on Quantum Molecular Resonance (QMR) and then subjected to in vitro recellularization with human bone marrow-derived mesenchymal stem cells (hBM-MSCs). Our results demonstrated the efficiency of decellularization treatment gaining a natural, non-immunogenic scaffold with preserved collagen microenvironment that displays a favorable support to hMSC engraftment, spreading and differentiation. Ultrastructural analysis showed that the micro-perforation procedure preserved the collagen mesh, increasing the osteoinductive potential for mesenchymal precursor cells. In conclusion, we developed a novel tissue engineering protocol to obtain a non-immunogenic mucoperiosteal scaffold suitable for allogenic transplantation and CL/P repair. The innovative micro-perforation procedure improving hMSC osteogenic differentiation potentially impacts for enhanced palatal bone regeneration leading to future clinical applications in humans.

الإتاحة: https://doi.org/10.1038/s41598-021-93951-wTest
https://www.nature.com/articles/s41598-021-93951-w.pdfTest
https://www.nature.com/articles/s41598-021-93951-wTest

المؤلفون: Gambino, M., Manfredi, P., La Riccia, A., Di Leone, L., Shweiki, F.

المصدر: European Urology Open Science ; volume 32, page S60-S61 ; ISSN 2666-1683

مصطلحات موضوعية: Urology

الإتاحة: https://doi.org/10.1016/s2666-1683Test(21)00813-2
https://api.elsevier.com/content/article/PII:S2666168321008132?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2666168321008132?httpAccept=text/plainTest

المؤلفون: Bassi, A., Henry, B.M., Pighi, L., Leone, L., Lippi, G.

المصدر: Journal of Hospital Infection ; volume 112, page 127-128 ; ISSN 0195-6701

مصطلحات موضوعية: Infectious Diseases, Microbiology (medical), General Medicine

الإتاحة: https://doi.org/10.1016/j.jhin.2021.02.020Test
https://api.elsevier.com/content/article/PII:S0195670121000797?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0195670121000797?httpAccept=text/plainTest

المؤلفون: Tortorella A., Leone L., Lombardi A., Pizzo E., Bosso A., Winter R., Petraccone L., Del Vecchio P., Oliva R.

المساهمون: Tortorella, A., Leone, L., Lombardi, A., Pizzo, E., Bosso, A., Winter, R., Petraccone, L., Del Vecchio, P., Oliva, R.

المصدر: Scientific Reports. 13

مصطلحات موضوعية: Multidisciplinary

الوصف: The misuse of antibiotics has led to the emergence of drug-resistant pathogens. Antimicrobial peptides (AMPs) may represent valuable alternative to antibiotics; nevertheless, the easy degradation due to environmental stress and proteolytic enzyme action, limits their use. So far, different strategies have been developed to overcome this drawback. Among them, glycosylation of AMPs represents a promising approach. In this work, we synthesized and characterized the N-glycosilated form of the antimicrobial peptide LL-III (g-LL-III). The N-acetylglucosamine (NAG) was covalently linked to the Asn residue and the interaction of g-LL-III with bacterial model membranes, together with its resistance to proteases, were investigated. Glycosylation did not affect the peptide mechanism of action and its biological activity against both bacteria and eukaryotic cells. Interestingly, a higher resistance to the activity of proteolytic enzymes was achieved. The reported results pave the way for the successful application of AMPs in medicine and biotechnological fields.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0be7294eaca50c1fcd012ab3b35e948fTest
https://doi.org/10.1038/s41598-023-29984-0Test

المؤلفون: De Cristofaro, V., Pellegrini, V., Salvati, M., Leone, L., Giacomantonio, M.

المساهمون: De Cristofaro, V., Pellegrini, V., Salvati, M., Leone, L., Giacomantonio, M.

مصطلحات موضوعية: acceptance of inequality, system justification, collective action, life satisfaction, employment, european social survey

الوصف: The present work proposes that the relation of employment conditions (i.e., unemployment and precarious work vs. permanent employment) with participation in collective action and satisfaction with life depends on the extent to which acceptance of inequality is high or low, and that collective action mediates the association between employment conditions and satisfaction with life. We analyzed data from the European Social Survey (Round 8, 2016) and found that (1) when acceptance of inequality is low (vs. high), employment disadvantage is positively related to engagement in collective action and, in turn, satisfaction with life; (2) employment disadvantage is negatively related to satisfaction with life, and this relation increases when acceptance of inequality is low (vs. high). This study generates findings of interest to inequality researchers by showing the relevance of acceptance of inequality for collective action and life satisfaction in the context of employment.

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:001003099000001; firstpage:1; lastpage:21; numberofpages:21; journal:ANALYSES OF SOCIAL ISSUES AND PUBLIC POLICY; https://hdl.handle.net/11573/1685649Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85161610189

الإتاحة: https://doi.org/10.1111/asap.12356Test
https://hdl.handle.net/11573/1685649Test

المؤلفون: Leone L., Munoz-Garcia A. B., D'Alonzo D., Pavone V., Nastri F., Lombardi A.

المساهمون: Leone, L., Munoz-Garcia, A. B., D'Alonzo, D., Pavone, V., Nastri, F., Lombardi, A.

مصطلحات موضوعية: Artificial enzyme, DFT calculation, Indole oxidation, Mechanistic studie, Metalloporphyrin, Oxidation catalysis

الوصف: Over the last decades, much effort has been devoted to the construction of protein and peptide-based metalloporphyrin catalysts capable of promoting difficult transformations with high selectivity. In this context, mechanistic studies are fundamental to elucidate all the factors that contribute to catalytic performances and product selectivity. In our previous work, we selected the synthetic peptide-porphyrin conjugate MnMC6*a as a proficient catalyst for indole oxidation, promoting the formation of a 3-oxindole derivative with unprecedented selectivity. In this work, we have evaluated the role of the metal ion in affecting reaction outcome, by replacing manganese with iron in the MC6*a scaffold. Even though product selectivity is not altered upon metal substitution, FeMC6*a shows a lower substrate conversion and prolonged reaction times with respect to its manganese analogue. Experimental and theoretical studies have enabled us to delineate the reaction free energy profiles for both catalysts, indicating different thermodynamic limiting steps, depending on the nature of the metal ion.

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:001038697400001; volume:246; 112298; firstpage:1; lastpage:10; numberofpages:10; journal:JOURNAL OF INORGANIC BIOCHEMISTRY; https://hdl.handle.net/11588/931784Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85164266055; https://www.sciencedirect.com/science/article/pii/S0162013423001800?via=ihubTest

الإتاحة: https://doi.org/10.1016/j.jinorgbio.2023.112298Test
https://hdl.handle.net/11588/931784Test
https://www.sciencedirect.com/science/article/pii/S0162013423001800?via=ihubTest

المؤلفون: Mandarino, F. V., Barchi, A., Leone, L., Fanti, L., Azzolini, F., Rosati, R., Elmore, U., Danese, S.

المصدر: ESGE Days 2023 ; Endoscopy ; ISSN 1438-8812

الإتاحة: https://doi.org/10.1055/s-0043-1765057Test

المؤلفون: Pellegrini, V., Leone, L., Giacomantonio, M.

المساهمون: Pellegrini, V., Leone, L., Giacomantonio, M.

مصطلحات موضوعية: populist attitude, conspiracy belief, anti-immigration attitude, voting behavior

الوصف: This data article provides descriptive and analytic exploration of the links between anti-immigration policies, ideological and political attitudes and voting in an Italian Sample. More specifically, the data set comprises measures of socio-political dispositions (e.g., Right-Wing Authoritarianism), social world views (e.g. Dangerous World Beliefs), populist attitudes, self-reported voting in the last Italian political elections (March 4, 2018), and conspiracy beliefs. The sample consists of 774 participants, mostly non-student adult individuals. Participants completed an anonymous questionnaire

العلاقة: info:eu-repo/semantics/altIdentifier/wos/WOS:000495104500170; volume:25; numberofpages:8; journal:DATA IN BRIEF; https://hdl.handle.net/11573/1286420Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85067609750

الإتاحة: https://doi.org/10.1016/j.dib.2019.104144Test
https://hdl.handle.net/11573/1286420Test

المؤلفون: Giannini G., Milazzo F. M., Battistuzzi G., Rosi A., Anastasi A. M., Petronzelli F., Albertoni C., Tei L., Leone L., Salvini L., De Santis R.

المساهمون: Giannini, G., Milazzo, F. M., Battistuzzi, G., Rosi, A., Anastasi, A. M., Petronzelli, F., Albertoni, C., Tei, L., Leone, L., Salvini, L., De Santis, R.

مصطلحات موضوعية: Cancer, Chelating ligand, Radionuclide therapy, Tenascin, Tenatumomab, Theranostic

الوصف: Tenatumomab is an anti-tenascin murine monoclonal antibody previously used in clinical trials for delivering radionuclides to tumors by both pre-targeting (biotinylated Tenatumomab within PAGRIT) and direct 131Iodine labeling approaches. Here we present the synthesis and in vitro characterization of three Tenatumomab con-jugates to bifunctional chelating agents (NHS-DOTA, NCS-DOTA and NCS-DTPA). Results indicate ST8198AA1(Tenatumomab-DOTAMA, derived by conjugation of NHS-DOTA), as the most promising candidate in terms ofconjugation rate andyield, stability,antigenimmunoreactivity andaffinity. Labeling efficiency of thedifferentchelators was investigated with a panel of cold metals indicating DOTAMA as the best chelator. Labeling ofTenatumomab-DOTAMA was then optimized with several metals and stability performed confirms suitability of this conjugate for further development. ST8198AA1 represents an improvement of the previous antibody forms because the labeling with radionuclides like177Lu or64Cu would allow theranostic applications in patientsbearing tenascin expressing tumors

وصف الملف: STAMPA

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/31208798; info:eu-repo/semantics/altIdentifier/wos/WOS:000476649400005; volume:27; firstpage:3248; lastpage:3253; numberofpages:6; journal:BIOORGANIC & MEDICINAL CHEMISTRY; http://hdl.handle.net/11579/104245Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85067197804; http://www.journals.elsevier.com/bioorganic-and-medicinal-chemistryTest/

الإتاحة: https://doi.org/10.1016/j.bmc.2019.05.047Test
http://hdl.handle.net/11579/104245Test
http://www.journals.elsevier.com/bioorganic-and-medicinal-chemistryTest/

المؤلفون: Spinelli M, Natale F, Rinaudo M, Leone L, Fusco S, Grassi C

المساهمون: Spinelli, Matteo, Natale, Francesca, Rinaudo, Marco, Leone, Lucia, Mezzogori, D, Fusco, Salvatore, Grassi, Claudio

مصطلحات موضوعية: CREB, synaptic plasticity, high fat diet, exosomes, memory deficits, BDNF, personalized medicine, epigenetics, Settore BIO/09 - FISIOLOGIA

الوصف: Overnutrition and metabolic disorders impair cognitive functions through molecular mechanisms still poorly understood. In mice fed with a high fat diet (HFD) we analysed the expression of synaptic plasticity‐related genes and the activation of cAMP response elementbinding protein (CREB)‐brain‐derived neurotrophic factor (BDNF)‐tropomyosin receptor kinase B (TrkB) signalling. We found that a HFD inhibited both CREB phosphorylation and the expression of a set of CREB target genes in the hippocampus. The intranasal administration of neural stem cell (NSC)‐derived exosomes (exo‐NSC) epigenetically restored the transcription of Bdnf, nNOS, Sirt1, Egr3, and RelA genes by inducing the recruitment of CREB on their regulatory sequences. Finally, exo‐NSC administration rescued both BDNF signalling and memory in HFD mice. Collectively, our findings highlight novel mechanisms underlying HFD‐related memory impairment and provide evidence of the potential therapeutic effect of exo‐NSC against metabolic disease‐related cognitive decline

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/33256199; info:eu-repo/semantics/altIdentifier/wos/WOS:000597515500001; volume:2020; issue:21; firstpage:N/A; lastpage:N/A; numberofpages:16; issueyear:2020; journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; http://hdl.handle.net/10807/164173Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85096709639

الإتاحة: https://doi.org/10.3390/ijms21238994Test
http://hdl.handle.net/10807/164173Test