المؤلفون: Maistriaux, Louis, Foulon, Vincent, Fievé, Lies, Xhema, Daela, Evrard, Robin, Manon, Julie, Coyette, Maude, Bouzin, Caroline, Poumay, Yves, Gianello, Pierre, Behets Wydemans, Catherine, Lengelé, Benoît

المساهمون: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - SSS/IREC/2IP - IREC Imaging Platform (2IP), UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique

المصدر: Frontiers in Bioengineering and Biotechnology, Vol. 11, p. 1295075 [1-18] (2024)

مصطلحات موضوعية: Biomedical Engineering, Histology, Bioengineering, Biotechnology

الوصف: INTRODUCTION: Nipple–areolar complex (NAC) reconstruction after breast cancer surgery is challenging and does not always provide optimal long-term esthetic results. Therefore, generating a NAC using tissue engineering techniques, such as a decellularization–recellularization process, is an alternative option to recreate a specific 3D NAC morphological unit, which is then covered with an in vitro regenerated epidermis and, thereafter, skin-grafted on the reconstructed breast. MATERIALS AND METHODS: Human NACs were harvested from cadaveric donors and decellularized using sequential detergent baths. Cellular clearance and extracellular matrix (ECM) preservation were analyzed by histology, as well as by DNA, ECM proteins, growth factors, and residual sodium dodecyl sulfate (SDS) quantification. In vivo biocompatibility was evaluated 30 days after the subcutaneous implantation of native and decellularized human NACs in rats. In vitro scaffold cytocompatibility was assessed by static seeding of human fibroblasts on their hypodermal side for 7 days, while human keratinocytes were seeded on the scaffold epidermal side for 10 days by using the reconstructed human epidermis (RHE) technique to investigate the regeneration of a new epidermis. RESULTS: The decellularized NAC showed a preserved 3D morphology and appeared white. After decellularization, a DNA reduction of 98.3% and the absence of nuclear and HLA staining in histological sections confirmed complete cellular clearance. The ECM architecture and main ECM proteins were preserved, associated with the detection and decrease in growth factors, while a very low amount of residual SDS was detected after decellularization. The decellularized scaffolds were in vivo biocompatible, fully revascularized, and did not induce the production of rat anti-human antibodies after 30 days of subcutaneous implantation. Scaffold in vitro cytocompatibility was confirmed by the increasing proliferation of seeded human fibroblasts during 7 days of culture, associated with a high ...

العلاقة: boreal:285098; http://hdl.handle.net/2078.1/285098Test; urn:EISSN:2296-4185

الإتاحة: https://doi.org/10.3389/fbioe.2023.1295075Test
http://hdl.handle.net/2078.1/285098Test

المؤلفون: Vettese, Julia, Manon, Julie, Chretien, Antoine, Evrard, Robin, Fievé, Lies, Schubert, Thomas, Lengelé, Benoît, Behets Wydemans, Catherine, Cornu, Olivier

المساهمون: UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie plastique

المصدر: Frontiers in bioengineering and biotechnology, (2024)

الوصف: Large bone defect regeneration remains a major challenge for orthopedic surgeons. Tissue engineering approaches are therefore emerging in order to overcome this limitation. However, these processes can alter some of essential native tissue properties such as intermolecular crosslinks of collagen triple helices, which are known for their essential role in tissue structure and function. We assessed the persistence of extracellular matrix (ECM) properties in human fascia lata (HFL) and periosteum (HP) after tissue engineering processes such as decellularization and sterilization. Harvested from cadaveric donors (N=3), samples from each HFL and HP were decellularized following five different chemical protocols with and without detergents (D1-D4 and D5, respectively). D1 to D4, consisted of different combinations of Triton, Sodium dodecyl sulfate and Deoxyribonuclease, while D5 is routinely used in the institutional tissue bank. Decellularized HFL tissues were further gamma-irradiated (minimum 25kGy) in order to study the impact of sterilization on the ECM. Polarized light microscopy (PLM) was used to estimate the thickness and density of collagen fibers. Tissue hydration and content of hydroxyproline, enzymatic crosslinks, and non-enzymatic crosslinks (pentosidine) were semiquantified with Raman spectroscopy. ELISA was also used to analyze the maintenance of the decorin (DCN), an important small leucine rich proteoglycan for fibrillogenesis. Among the decellularization protocols, detergent-free treatments tended to further disorganize HFL samples, as more thin fibers (+53.7%) and less thick ones (-32.6%) were recorded, as well as less collagen enzymatic crosslinks (-25.2%, p = 0.19) and a significant decrease of DCN (p = 0.036). GAG content was significantly reduced in both tissue types after all decellularization protocols. On the other hand, HP samples, were more sensitive to the D1 detergent-based treatments, with more disrupted collagen organization and greater, though not significant, loss of enzymatic ...

العلاقة: boreal:285911; http://hdl.handle.net/2078.1/285911Test; urn:EISSN:2296-4185

الإتاحة: http://hdl.handle.net/2078.1/285911Test

المؤلفون: Maistriaux, Louis, Foulon, Vincent, Fievé, Lies, Xhema, Daela, Evrard, Robin, Manon, Julie, Coyette, Maude, Bouzin, Caroline, Poumay, Yves, Gianello, Pierre, Behets Wydemans, Catherine, Lengelé, Benoît

المساهمون: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - SSS/IREC/2IP - IREC Imaging Platform (2IP), UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique

المصدر: Frontiers in Bioengineering and Biotechnology, Vol. 11, p. 1295075 [1-18] (2024)

مصطلحات موضوعية: Biomedical Engineering, Histology, Bioengineering, Biotechnology

الوصف: INTRODUCTION: Nipple–areolar complex (NAC) reconstruction after breast cancer surgery is challenging and does not always provide optimal long-term esthetic results. Therefore, generating a NAC using tissue engineering techniques, such as a decellularization–recellularization process, is an alternative option to recreate a specific 3D NAC morphological unit, which is then covered with an in vitro regenerated epidermis and, thereafter, skin-grafted on the reconstructed breast. MATERIALS AND METHODS: Human NACs were harvested from cadaveric donors and decellularized using sequential detergent baths. Cellular clearance and extracellular matrix (ECM) preservation were analyzed by histology, as well as by DNA, ECM proteins, growth factors, and residual sodium dodecyl sulfate (SDS) quantification. In vivo biocompatibility was evaluated 30 days after the subcutaneous implantation of native and decellularized human NACs in rats. In vitro scaffold cytocompatibility was assessed by static seeding of human fibroblasts on their hypodermal side for 7 days, while human keratinocytes were seeded on the scaffold epidermal side for 10 days by using the reconstructed human epidermis (RHE) technique to investigate the regeneration of a new epidermis. RESULTS: The decellularized NAC showed a preserved 3D morphology and appeared white. After decellularization, a DNA reduction of 98.3% and the absence of nuclear and HLA staining in histological sections confirmed complete cellular clearance. The ECM architecture and main ECM proteins were preserved, associated with the detection and decrease in growth factors, while a very low amount of residual SDS was detected after decellularization. The decellularized scaffolds were in vivo biocompatible, fully revascularized, and did not induce the production of rat anti-human antibodies after 30 days of subcutaneous implantation. Scaffold in vitro cytocompatibility was confirmed by the increasing proliferation of seeded human fibroblasts during 7 days of culture, associated with a high ...

العلاقة: boreal:285098; http://hdl.handle.net/2078.1/285098Test; urn:EISSN:2296-4185

الإتاحة: https://doi.org/10.3389/fbioe.2023.1295075Test
http://hdl.handle.net/2078.1/285098Test

المؤلفون: Vettese, Julia, Manon, Julie, Chretien, Antoine, Evrard, Robin, Fievé, Lies, Schubert, Thomas, Lengelé, Benoît, Behets Wydemans, Catherine, Cornu, Olivier

المساهمون: UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie plastique

المصدر: Frontiers in bioengineering and biotechnology, (2024)

الوصف: Large bone defect regeneration remains a major challenge for orthopedic surgeons. Tissue engineering approaches are therefore emerging in order to overcome this limitation. However, these processes can alter some of essential native tissue properties such as intermolecular crosslinks of collagen triple helices, which are known for their essential role in tissue structure and function. We assessed the persistence of extracellular matrix (ECM) properties in human fascia lata (HFL) and periosteum (HP) after tissue engineering processes such as decellularization and sterilization. Harvested from cadaveric donors (N=3), samples from each HFL and HP were decellularized following five different chemical protocols with and without detergents (D1-D4 and D5, respectively). D1 to D4, consisted of different combinations of Triton, Sodium dodecyl sulfate and Deoxyribonuclease, while D5 is routinely used in the institutional tissue bank. Decellularized HFL tissues were further gamma-irradiated (minimum 25kGy) in order to study the impact of sterilization on the ECM. Polarized light microscopy (PLM) was used to estimate the thickness and density of collagen fibers. Tissue hydration and content of hydroxyproline, enzymatic crosslinks, and non-enzymatic crosslinks (pentosidine) were semiquantified with Raman spectroscopy. ELISA was also used to analyze the maintenance of the decorin (DCN), an important small leucine rich proteoglycan for fibrillogenesis. Among the decellularization protocols, detergent-free treatments tended to further disorganize HFL samples, as more thin fibers (+53.7%) and less thick ones (-32.6%) were recorded, as well as less collagen enzymatic crosslinks (-25.2%, p = 0.19) and a significant decrease of DCN (p = 0.036). GAG content was significantly reduced in both tissue types after all decellularization protocols. On the other hand, HP samples, were more sensitive to the D1 detergent-based treatments, with more disrupted collagen organization and greater, though not significant, loss of enzymatic ...

العلاقة: boreal:285911; http://hdl.handle.net/2078.1/285911Test; urn:EISSN:2296-4185

الإتاحة: http://hdl.handle.net/2078.1/285911Test

المؤلفون: Evrard, Robin, Manon, Julie, Cornu, Olivier, Schubert, Thomas, Lengelé, Benoît, American Transplant Congress (ATC) 2023

المساهمون: UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie plastique

المصدر: American Journal of Transplantation, Vol. 23, no. 6 Suppl 1, p. S1082 (2023)

العلاقة: boreal:279308; http://hdl.handle.net/2078.1/279308Test; urn:ISSN:1600-6135; urn:EISSN:1600-6143

الإتاحة: http://hdl.handle.net/2078.1/279308Test

المؤلفون: Manon, Julie, Evrard, Robin, Maistriaux, Louis, Fievé, Lies, de Ville de Goyet, Christine, Vanham, Philippine, Schubert, Thomas, Gianello, Pierre, Lengelé, Benoît, Behets Wydemans, Catherine, Cornu, Olivier, The International Combined Orthopaedic Research Societies (ICORS), World Congress of Orthopaedic Research

المساهمون: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie et transplantation abdominale, UCL - (SLuc) Service de chirurgie plastique

المصدر: Orthopaedic Proceedings, Vol. 105-B, no.SUPP_8, p. 10-10 (2023)

الوصف: Periosteal mesenchymal stem cells (PMSC) are an emerging niche of stem cells to enhance bone healing by tissue engineering process. They have to be differentiated into osteoprogenitors in order to synthesize new bone matrix. In vitro differentiation with specific differentiation medium (DM) is not exactly representative of what occurs in vivo. The interaction between PMSC and growth factors (GF) present in biological matrix is somewhat less understood. The goal of this study is to explore the possibility of spontaneous PMSC differentiation in contact with different biological matrices without DM. [.]

العلاقة: boreal:279313; http://hdl.handle.net/2078.1/279313Test; urn:ISSN:1358-992X; urn:EISSN:2049-4416

الإتاحة: https://doi.org/10.1302/1358-992x.2023.8.010Test
http://hdl.handle.net/2078.1/279313Test

المؤلفون: Evrard, Robin, Maistriaux, Louis, Manon, Julie, Rafferty, Chiara, Cornu, Olivier, Gianello, Pierre, Lengelé, Benoît, Schubert, Thomas, The International Combined Orthopaedic Research Societies (ICORS), World Congress of Orthopaedic Research

المساهمون: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie plastique, UCL - (SLuc) Service de chirurgie et transplantation abdominale

المصدر: Orthopaedic Proceedings, Vol. 105-B, no.SUPP_7, p. 40-40 (2023)

الوصف: The purpose of this study is to enhance massive bone allografts osseointegration used to reconstruct large bone defects. These allografts show >50% complication rate requiring surgical revision in 20% cases. A new protocol for total bone decellularisation exploiting the vasculature can offer a reduction of postoperative complication by annihilating immune response and improving cellular colonization/ osseointegration. [.]

العلاقة: boreal:279312; http://hdl.handle.net/2078.1/279312Test; urn:ISSN:1358-992X; urn:EISSN:2049-4416

الإتاحة: https://doi.org/10.1302/1358-992x.2023.7.040Test
http://hdl.handle.net/2078.1/279312Test

المؤلفون: Manon, Julie, Evrard, Robin, Fievé, Lies, Xhema, Daela, Maistriaux, Louis, Schubert, Thomas, Lengelé, Benoît, Behets Wydemans, Catherine, Cornu, Olivier, American Transplant Congress 2023

المساهمون: UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie plastique

المصدر: American Journal of Transplantation, Vol. 23, no.6 Suppl 1, p. S1080 (2023)

الوصف: PURPOSE: Decellularization procedures significantly progressed to reduce the risk of immune rejection in allo- or xenogenic transplantation. The validation of decellularization protocols is typically based on Crapo’s criteria, including the absence of visible nuclei on hematoxylin and eosin (H&E) or Hoechst staining and a DNA content lower than 50ng/mg dry weight. Our study compared five decellularization protocols in order to determine the optimal approach, but raised the question of why recipients can still become immunized despite meeting validation criteria. [.]

العلاقة: boreal:285101; http://hdl.handle.net/2078.1/285101Test; urn:ISSN: 1600-6135; urn:EISSN:1600-6143

الإتاحة: http://hdl.handle.net/2078.1/285101Test

المؤلفون: Evrard, Robin, Manon, Julie, Cornu, Olivier, Schubert, Thomas, Lengelé, Benoît, American Transplant Congress (ATC) 2023

المساهمون: UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie plastique

المصدر: American Journal of Transplantation, Vol. 23, no. 6 Suppl 1, p. S1082 (2023)

العلاقة: boreal:279308; http://hdl.handle.net/2078.1/279308Test; urn:ISSN:1600-6135; urn:EISSN:1600-6143

الإتاحة: http://hdl.handle.net/2078.1/279308Test

المؤلفون: Evrard, Robin, Maistriaux, Louis, Manon, Julie, Rafferty, Chiara, Cornu, Olivier, Gianello, Pierre, Lengelé, Benoît, Schubert, Thomas, The International Combined Orthopaedic Research Societies (ICORS), World Congress of Orthopaedic Research

المساهمون: UCL - SSS/IREC/CHEX - Pôle de chirgurgie expérimentale et transplantation, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/NMSK - Neuro-musculo-skeletal Lab, UCL - (SLuc) Service d'orthopédie et de traumatologie de l'appareil locomoteur, UCL - (SLuc) Service de chirurgie plastique, UCL - (SLuc) Service de chirurgie et transplantation abdominale

المصدر: Orthopaedic Proceedings, Vol. 105-B, no.SUPP_7, p. 40-40 (2023)

الوصف: The purpose of this study is to enhance massive bone allografts osseointegration used to reconstruct large bone defects. These allografts show >50% complication rate requiring surgical revision in 20% cases. A new protocol for total bone decellularisation exploiting the vasculature can offer a reduction of postoperative complication by annihilating immune response and improving cellular colonization/ osseointegration. [.]

العلاقة: boreal:279312; http://hdl.handle.net/2078.1/279312Test; urn:ISSN:1358-992X; urn:EISSN:2049-4416

الإتاحة: https://doi.org/10.1302/1358-992x.2023.7.040Test
http://hdl.handle.net/2078.1/279312Test