نتائج البحث - "Laudański, Piotr"

1

دورية أكاديمية

Targeted gene expression profiling for accurate endometrial receptivity testing

المؤلفون: Meltsov, Alvin, Saare, Merli, Teder, Hindrek, Paluoja, Priit, Arffman, Riikka K., Piltonen, Terhi, Laudanski, Piotr, Wielgos, Miroslaw, Gianaroli, Luca, Koel, Mariann, Peters, Maire, Salumets, Andres, Krjutskov, Kaarel, Palta, Priit

المساهمون: Institute for Molecular Medicine Finland, Genomics of Neurological and Neuropsychiatric Disorders

مصطلحات موضوعية: Embryo-transfer, Frozen, Cycles, Window, Array, 1184 Genetics, developmental biology, physiology

الوصف: Expressional profiling of the endometrium enables the personalised timing of the window of implantation (WOI). This study presents and evaluates a novel analytical pipeline based on a TAC-seq (Targeted Allele Counting by sequencing) method for endometrial dating. The expressional profiles were clustered, and differential expression analysis was performed on the model development group, using 63 endometrial biopsies spanning over proliferative (PE, n = 18), early-secretory (ESE, n = 18), mid-secretory (MSE, n = 17) and late-secretory (LSE, n = 10) endometrial phases of the natural cycle. A quantitative predictor model was trained on the development group and validated on sequenced samples from healthy women, consisting of 52 paired samples taken from ESE and MSE phases and five LSE phase samples from 31 individuals. Finally, the developed test was applied to 44 MSE phase samples from a study group of patients diagnosed with recurrent implantation failure (RIF). In validation samples (n = 57), we detected displaced WOI in 1.8% of the samples from fertile women. In the RIF study group, we detected a significantly higher proportion of the samples with shifted WOI than in the validation set of samples from fertile women, 15.9% and 1.8% (p = 0.012), respectively. The developed model was evaluated with an average cross-validation accuracy of 98.8% and an accuracy of 98.2% in the validation group. The developed beREADY screening model enables sensitive and dynamic detection of selected transcriptome biomarkers, providing a quantitative and accurate prediction of endometrial receptivity status. ; Peer reviewed

وصف الملف: application/pdf

العلاقة: This research was funded by the Estonian Research Council (grant PRG1076), Horizon 2020 innovation grant (ERIN, grant no. EU952516), the EU-FP7 Marie Curie Industry-Academia Partnerships and Pathways (IAPP, grant SARM, EU324509), the Enterprise Estonia (grant no. EU48695) and by Polish Ministry of Health (grant no. 6/6/4/1/NPZ/2017/1210/1352).; Meltsov , A , Saare , M , Teder , H , Paluoja , P , Arffman , R K , Piltonen , T , Laudanski , P , Wielgos , M , Gianaroli , L , Koel , M , Peters , M , Salumets , A , Krjutskov , K & Palta , P 2023 , ' Targeted gene expression profiling for accurate endometrial receptivity testing ' , Scientific Reports , vol. 13 , no. 1 , 13959 . https://doi.org/10.1038/s41598-023-40991-zTest; ORCID: /0000-0001-9320-7008/work/153479395; 85168754103; bc8306b3-aa36-4111-a28b-8f300fa2ab2b; http://hdl.handle.net/10138/571101Test; 001138684400001

الإتاحة: http://hdl.handle.net/10138/571101Test

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2

دورية أكاديمية

The Use of the Neoglycolipid-Based Oligosaccharide Microarray System in the Diagnosis of Endometriosis – Preliminary Study

المؤلفون: Wojtyla, Cezary, Tołwiński, Ignacy, Laudański, Piotr

المصدر: Journal of Inflammation Research ; volume Volume 17, page 899-908 ; ISSN 1178-7031

الإتاحة: https://doi.org/10.2147/jir.s439709Test
https://www.dovepress.com/getfile.php?fileID=96651Test

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3

دورية أكاديمية

Is the leptin/BMI ratio a reliable biomarker for endometriosis?

المصدر: Frontiers in Endocrinology ; volume 15 ; ISSN 1664-2392

مصطلحات موضوعية: Endocrinology, Diabetes and Metabolism

الوصف: Background The aim of this study was to analyze the concentration of leptin in peritoneal fluid and plasma and to assess their role as potential biomarkers in the diagnosis of endometriosis. Materials & methods Leptin adjusted for BMI (leptin/BMI ratio) was measured using surface plasmon resonance imaging (SPRI) biosensors. Patients with suspected endometriosis were included in the study. Plasma was collected from 70 cases, and peritoneal fluid from 67 cases. Based on the presence of endometriosis lesions detected during laparoscopy, patients were divided into a study group and a control group (patients without endometriosis). Results Leptin/BMI ratio in plasma did not differ between women with endometriosis and the control group (0.7159 ± 0.259 vs 0.6992 ± 0.273, p= 0,7988). No significant differences were observed in peritoneal leptin/BMI ratio levels in patients with and without endometriosis (0.6206 ± 0.258 vs 0.6215 ± 0.264, p= 0,9896). Plasma and peritoneal leptin/BMI ratios were significantly lower in women with endometriosis - related primary infertility compared to women with endometriosis without primary infertility (0.640 ± 0.502 vs 0.878 ± 0.623, p < 0.05). The difference was observed in case of primary infertility, but not in terms of the secondary one. No significant differences were noted between leptin/BMI ratio in the proliferative phase and the secretory phase (0.716 ± 0.252 vs 0.697 ± 0.288, p= 0,7785). Conclusion The results of present study do not support the relevance of leptin concentration determination as a biomarker of the endometriosis. Due to the limited number of samples in the tested group, further studies are needed to confirm its role.

الإتاحة: https://doi.org/10.3389/fendo.2024.1359182Test

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4

دورية أكاديمية

Plasma and Peritoneal Fluid Annexin A2 Levels in Patients with Endometriosis

مصطلحات موضوعية: Journal of Inflammation Research

الوصف: Michal Zygarowicz,1,* Joanna Kacperczyk-Bartnik,2,3,* Janusz Sierdzinski,4 Cezary Wojtyla,5,6 Piotr Pierzynski,5,6 Grzegorz Manka,7 Mariusz Kiecka,7 Robert Z Spaczynski,8 Piotr Piekarski,9 Beata Banaszewska,10 Artur Jakimiuk,11,12 Tadeusz Issat,13 Wojciech Rokita14,15 ,â€ Jakub Mlodawski,14,15 Maria Szubert,3,16,17 Piotr Sieroszewski,16,18 Grzegorz Raba,19,20 Kamil Szczupak,19,20 Tomasz Kluz,21 Marek Kluza,21 Michal Lipa,3,22 Damian Warzecha,5,22 Miroslaw Wielgos,23,24 Ewa Koc-Zorawska,25,26 Marcin Zorawski,26,27 Piotr Laudanski5,6,28 1Studentsâ€™ Scientific Group Affiliated to the Department of Obstetrics, Gynecology and Gynecological Oncology, Medical University of Warsaw, Warsaw, Poland; 2II Department of Obstetrics and Gynaecology, Medical University of Warsaw, Warsaw, Poland; 3Club 35. Polish Society of Gynecologists and Obstetricians, Wrocław, Poland; 4Department of Medical Informatics and Telemedicine, Medical University of Warsaw, Warsaw, Poland; 5OVIklinika Infertility Center, Warsaw, Poland; 6Womenâ€™s Health Research Institute, Calisia University, Kalisz, Poland; 7Angelius Provita Hospital, Katowice, Poland; 8Center for Gynecology, Obstetrics and Infertility Treatment Pastelova, Poznan, Poland; 9Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, Poznan, Poland; 10Chair and Department of Laboratory Diagnostics, Poznan University of Medical Sciences, Poznan, Poland; 11Department of Obstetrics and Gynecology, Central Clinical Hospital of the Ministry of Interior, Warsaw, Poland; 12Center of Reproductive Health, Institute of Mother and Child in Warsaw, Warsaw, Poland; 13Department of Obstetrics and Gynecology, Institute of Mother and Child in Warsaw, Warsaw, Poland; 14Collegium Medicum Jan Kochanowski University in Kielce, Kielce, Poland; 15Clinic of Obstetrics and Gynecology, Provincial Combined Hospital in Kielce, Kielce, Poland; 16Department of Gynecology and Obstetrics Medical University of ...

وصف الملف: text/html

العلاقة: https://www.dovepress.com/plasma-and-peritoneal-fluid-annexin-a2-levels-in-patients-with-endomet-peer-reviewed-fulltext-article-JIRTest

الإتاحة: https://doi.org/10.2147/JIR.S421389Test
https://www.dovepress.com/plasma-and-peritoneal-fluid-annexin-a2-levels-in-patients-with-endomet-peer-reviewed-fulltext-article-JIRTest

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5

دورية أكاديمية

Targeted gene expression profiling for accurate endometrial receptivity testing

المؤلفون: Meltsov, Alvin, Saare, Merli, Teder, Hindrek, Paluoja, Priit, Arffman, Riikka K., Piltonen, Terhi, Laudanski, Piotr, Wielgoś, Mirosław, Gianaroli, Luca, Koel, Mariann, Peters, Maire, Salumets, Andres, Krjutškov, Kaarel, Palta, Priit

المساهمون: Enterprise Estonia, Estonian Research Council, Polish Ministry of Health, Horizon 2020 innovation grant, EU-FP7 Marie Curie Industry-Academia Partnerships and Pathways

المصدر: Scientific Reports ; volume 13, issue 1 ; ISSN 2045-2322

مصطلحات موضوعية: Multidisciplinary

الوصف: Expressional profiling of the endometrium enables the personalised timing of the window of implantation (WOI). This study presents and evaluates a novel analytical pipeline based on a TAC-seq (Targeted Allele Counting by sequencing) method for endometrial dating. The expressional profiles were clustered, and differential expression analysis was performed on the model development group, using 63 endometrial biopsies spanning over proliferative (PE, n = 18), early-secretory (ESE, n = 18), mid-secretory (MSE, n = 17) and late-secretory (LSE, n = 10) endometrial phases of the natural cycle. A quantitative predictor model was trained on the development group and validated on sequenced samples from healthy women, consisting of 52 paired samples taken from ESE and MSE phases and five LSE phase samples from 31 individuals. Finally, the developed test was applied to 44 MSE phase samples from a study group of patients diagnosed with recurrent implantation failure (RIF). In validation samples (n = 57), we detected displaced WOI in 1.8% of the samples from fertile women. In the RIF study group, we detected a significantly higher proportion of the samples with shifted WOI than in the validation set of samples from fertile women, 15.9% and 1.8% (p = 0.012), respectively. The developed model was evaluated with an average cross-validation accuracy of 98.8% and an accuracy of 98.2% in the validation group. The developed beREADY screening model enables sensitive and dynamic detection of selected transcriptome biomarkers, providing a quantitative and accurate prediction of endometrial receptivity status.

الإتاحة: https://doi.org/10.1038/s41598-023-40991-zTest
https://www.nature.com/articles/s41598-023-40991-z.pdfTest
https://www.nature.com/articles/s41598-023-40991-zTest

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6

دورية أكاديمية

The role of miRNA molecules in the miscarriage process

المؤلفون: Omeljaniuk, Wioleta Justyna, Laudański, Piotr, Miltyk, Wojciech

المصدر: Biology of Reproduction ; volume 109, issue 1, page 29-44 ; ISSN 0006-3363 1529-7268

مصطلحات موضوعية: Cell Biology, General Medicine, Reproductive Medicine

الوصف: The etiology and pathogenesis of miscarriage, which is the most common pregnancy complication, have not been fully elucidated. There is a constant search for new screening biomarkers that would allow for the early diagnosis of disorders associated with pregnancy pathology. The profiling of microRNA expression is a promising research area, which can help establish the predictive factors for pregnancy diseases. Molecules of microRNAs are involved in several processes crucial for the development and functioning of the body. These processes include cell division and differentiation, programmed cell death, blood vessel formation or tumorigenesis, and the response to oxidative stress. The microRNAs affect the number of individual proteins in the body due to their ability to regulate gene expression at the post-transcriptional level, ensuring the normal course of many cellular processes. Based on the scientific facts available, this paper presents a compendium on the role of microRNA molecules in the miscarriage process. The expression of potential microRNA molecules as early minimally invasive diagnostic biomarkers may be evaluated as early as the first weeks of pregnancy and may constitute a monitoring factor in the individual clinical care of women in early pregnancy, especially after the first miscarriage. To summarize, the described scientific data set a new direction of research in the development of preventive care and prognostic monitoring of the course of pregnancy.

الإتاحة: https://doi.org/10.1093/biolre/ioad047Test
https://academic.oup.com/biolreprod/article-pdf/109/1/29/51472958/ioad047.pdfTest

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7

دورية أكاديمية

Plasma and Peritoneal Fluid Annexin A2 Levels in Patients with Endometriosis

المصدر: Journal of Inflammation Research ; volume Volume 16, page 5959-5969 ; ISSN 1178-7031

الإتاحة: https://doi.org/10.2147/jir.s421389Test
https://www.dovepress.com/getfile.php?fileID=95005Test

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8

دورية أكاديمية

Placental Cannabinoid Receptor Expression in Preterm Birth.

المؤلفون: Feduniw, Stepan, Krupa, Izabela, Łagowska, Katarzyna, Laudański, Piotr, Tabarkiewicz, Jacek, Stawarz, Barbara, Raba, Grzegorz

المصدر: Journal of Pregnancy; 5/7/2024, Vol. 2024, p1-10, 10p

مستخلص: Background: The cannabinoid receptor (CBR) plays a significant role in oogenesis, pregnancy, and childbirth. It might also play a significant role in preterm birth (PTB). The aim of the study was to investigate the association between the expression of the CBR in the placenta and the incidence of PTB. Methods: This prospective, observational, multicentre preliminary study was conducted on placental samples obtained from 109 women. The study included 95 patients hospitalized due to the high risk of PTB. They were divided into two groups: Group 1, where the expression of the CBR1 and CBR1a was analyzed, and Group 2, in which we examined CBR2 expression. The control group, that is, Group 3, consisted of 14 women who delivered at term, and their placentas were tested for the presence of all three receptor types (CBR1, CBR1a, and CBR2). Results: The study used reverse transcription and real-time PCR methods to assess the expression of CBRs in the placental tissues. The expression of the CBR2, CBR1, and CBR1a receptors was significantly lower in the placentas of women after PTB compared to those after term births, p = 0.038 , 0.033, and 0.034, respectively. Conclusions: The presence of CBR mRNA in the human placental tissue was confirmed. The decreased expression of CBRs could serve as an indicator in predicting PTB. [ABSTRACT FROM AUTHOR]

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