المؤلفون: Arya, S, Naburi, H, Kawaza, K, Newton, S, Anyabolu, CH, Bergman, N, Rao, SPN, Mittal, P, Assenga, E, Gadama, L, Larsen-Reindorf, R, Kuti, O, Linner, A, Yoshida, S, Chopra, N, Ngarina, M, Msusa, AT, Boakye-Yiadom, A, Kuti, BP, Morgan, B, Minckas, N, Suri, J, Moshiro, R, Samuel, V, Wireko-Brobby, N, Rettedal, S, Jaiswal, HV, Sankar, MJ, Nyanor, I, Tiwary, H, Anand, P, Manu, AA, Nagpal, K, Ansong, D, Saini, I, Aggarwal, KC, Wadhwa, N, Bahl, R, Westrup, B, Adejuyigbe, EA, Plange-Rhule, G, Dube, Q, Chellani, H, Massawe, A

المصدر: The New England journal of medicine. 384(21):2028-2038

مصطلحات موضوعية: Medicin och hälsovetenskap

الوصول الحر: http://kipublications.ki.se/Default.aspx?queryparsed=id:147006179Test

المؤلفون: Urizar, Adriana Ibarra, Prause, Michala, Ingerslev, Lars Roed, Wortham, Matthew, Sui, Yinghui, Sander, Maike, Williams, Kristine, Barrès, Romain, Larsen, Martin R, Christensen, Gitte Lund, Billestrup, Nils

المصدر: Cell Death & Disease. 14(7)

مصطلحات موضوعية: Genetics, Diabetes, Underpinning research, 2.1 Biological and endogenous factors, Aetiology, 1.1 Normal biological development and functioning, Metabolic and endocrine, Animals, Mice, Bone Morphogenetic Protein 2, Chromatin, Diabetes Mellitus, Type 2, Histone Code, Protein Processing, Post-Translational, Signal Transduction, Biochemistry and Cell Biology, Oncology and Carcinogenesis

الوصف: Insufficient insulin secretion is a hallmark of type 2 diabetes and has been attributed to beta cell identity loss characterized by decreased expression of several key beta cell genes. The pro-inflammatory factor BMP-2 is upregulated in islets of Langerhans from individuals with diabetes and acts as an inhibitor of beta cell function and proliferation. Exposure to BMP-2 induces expression of Id1-4, Hes-1, and Hey-1 which are transcriptional regulators associated with loss of differentiation. The aim of this study was to investigate the mechanism by which BMP-2 induces beta cell dysfunction and loss of cell maturity. Mouse islets exposed to BMP-2 for 10 days showed impaired glucose-stimulated insulin secretion and beta cell proliferation. BMP-2-induced beta cell dysfunction was associated with decreased expression of cell maturity and proliferation markers specific to the beta cell such as Ins1, Ucn3, and Ki67 and increased expression of Id1-4, Hes-1, and Hey-1. The top 30 most regulated proteins significantly correlated with corresponding mRNA expression. BMP-2-induced gene expression changes were associated with a predominant reduction in acetylation of H3K27 and a decrease in NeuroD1 chromatin binding activity. These results show that BMP-2 induces loss of beta cell maturity and suggest that remodeling of H3K27ac and decreased NeuroD1 DNA binding activity participate in the effect of BMP-2 on beta cell dysfunction.

وصف الملف: application/pdf

الوصول الحر: https://escholarship.org/uc/item/76z1898cTest

المؤلفون: Børsen-Koch, V. Aguirre, Rørsted, J. L., Justesen, A. B., Stokholm, A., Verma, K., Winther, M. L., Knudstrup, E., Nielsen, K. B., Sahlholdt, C., Larsen, J. R., Cassisi, S., Serenelli, A. M., Casagrande, L., Christensen-Dalsgaard, J., Davies, G. R., Ferguson, J. W., Lund, M. N., Weiss, A., White, T. R.

مصطلحات موضوعية: Astrophysics - Solar and Stellar Astrophysics, Astrophysics - Earth and Planetary Astrophysics, Astrophysics - Astrophysics of Galaxies

الوصف: We introduce the public version of the BAyesian STellar Algorithm (BASTA), an open-source code written in {\tt Python} to determine stellar properties based on a set of astrophysical observables. BASTA has been specifically designed to robustly combine large datasets that include asteroseismology, spectroscopy, photometry, and astrometry. We describe the large number of asteroseismic observations that can be fit by the code and how these can be combined with atmospheric properties (as well as parallaxes and apparent magnitudes), making it the most complete analysis pipeline available for oscillating main-sequence, subgiant, and red giant stars. BASTA relies on a set of pre-built stellar isochrones or a custom-designed library of stellar tracks which can be further refined using our interpolation method (both along and across stellar tracks/isochrones). We perform recovery tests with simulated data that reveal levels of accuracy at the few percent level for radii, masses, and ages when individual oscillation frequencies are considered, and show that asteroseismic ages with statistical uncertainties below 10% are within reach if our stellar models are reliable representations of stars. BASTA is extensively documented and includes a suite of examples to support easy adoption and further development by new users.
Comment: 21 pages, 14 figures, resubmitted after positive referee report. The code is available at https://github.com/BASTAcode/BASTATest

الوصول الحر: http://arxiv.org/abs/2109.14622Test

المؤلفون: Dennis, Matthew, Angst, Christof, Larsen, Joshua R., Larsen, A.

مصطلحات موضوعية: Life Science

الوصف: This study describes the first national comprehensive model of beaver dam likelihood and associated floodplain development. The subsequent delineation of areas reflecting overall opportunities and conflict reveal that the reintroduction of Castor fiber into Switzerland implies a net benefit from a landscape restoration perspective. Though this is the case measured at the national-scale, we identified concentrated regions of potential land-use conflict where mitigation and management should be focussed. The development of similar models in other contexts, for example, where planned beaver reintroductions are being prepared, could help maximise landscape restoration goals whilst minimising undesirable land-use conflicts which may harm conservation efforts.

وصف الملف: text/html

العلاقة: https://edepot.wur.nl/660409Test; https://research.wur.nl/en/publications/a-spatially-explicit-model-of-beaver-river-floodplains-understandTest

الإتاحة: https://doi.org/10.5281/zenodo.10935698Test
https://research.wur.nl/en/publications/a-spatially-explicit-model-of-beaver-river-floodplains-understandTest

المؤلفون: Beltrán Camacho, Lucía, Bhosale, S.D., Sánchez Morillo, Daniel, Sánchez Gomar, Ismael, Rojas Torres, Marta, Eslava Alcon, Sara, Martínez-Torija, Mario, Ruiz de Infante, Mª Angeles, Nieto-Martín, Mª Dolores, Rodríguez Iglesias, Manuel Antonio, Moreno, Juan A., Berrocoso Domínguez, Esther María, Larsen, Martin R., Moreno-Luna, Rafael, Durán Ruiz, María del Carmen

المساهمون: Anatomía Patológica, Biología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología, Biomedicina, Biotecnología y Salud Pública, Ingeniería en Automática, Electrónica, Arquitectura y Redes de Computadores, Neurociencias

المصدر: Int J Biol Sci 2023; 19(6):1664-1680

مصطلحات موضوعية: COVID-19, ECFCs, Viral infection, RNA metabolism, cardiovascular disease, endothelial dysfunction, autophagy, proteomics, mass spectrometry

الوصف: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection significantly affects the cardiovascular system, causing vascular damage and thromboembolic events in critical patients. Endothelial dysfunction represents one of the first steps in response to COVID-19 that might lead to cardiovascular complications and long-term sequelae. However, despite the enormous efforts in the last two years, the molecular mechanisms involved in such processes remain poorly understood. Herein, we analyzed the protein changes taking place in endothelial colony forming cells (ECFCs) after the incubation with the serum from individuals infected with COVID-19, whether asymptomatic or critical patients, by application of a label free-quantitative proteomics approach. Specifically, ECFCs from healthy individuals were incubated ex-vivo with the serum of either COVID-19 negative donors (PCR-/IgG-, n:8), COVID-19 asymptomatic donors at different infective stages (PCR+/ IgG-, n:8and PCR-/IgG+, n:8), or hospitalized critical COVID-19 patients (n:8), followed by proteomics analysis. In total, 590 proteins were differentially expressed in ECFCs in response to all infected serums. Predictive analysis highlighted several proteins like CAPN5, SURF4, LAMP2 or MT-ND1, as highly discriminating features between the groups compared. Protein changes correlated with viral infection, RNA metabolism or autophagy, among others. Remarkably, the angiogenic potential of ECFCs in response to the infected serums was impaired, and many of the protein alterations in response to the serum of critical patients were associated with cardiovascular-related pathologies. ; This study was supported by GLOBALCAJA-Ayuda COVID-19; and Fondo Supera COVID-19, funded by Banco Santander and CRUE universidades, Ref. IPSA-COVID-19, and the Institute of Health Carlos III, ISCIII (PI18-00427, PI20-00716), co-funded by European Regional Development “A way to make Europe”.

وصف الملف: application/pdf

العلاقة: info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 (ISCIII)/PI18%2F00427/ES/ESTUDIO PRECLINICO: USO COMBINADO DE CELULAS MADRES%2FPROGENITORAS ENDOTELIALES DERIVADAS DEL TEJIDO ADIPOSO DE LESIONADOS MEDULARES PARA LA REPARACION%2FREGENERACION DE ULCERAS POR PRESION/; info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 (ISCIII)/PI20%2F00716/ES/COMPLICACIONES VASCULARES EN DIABETES MELLITUS: APLICACION DE ESTRATEGIAS PROTEOMICAS PARA POTENCIAR EL DESARROLLO DE TERAPIAS MOLECULARES%2FCELULARES ESPECIFICAS PARA CADA PACIENTE/; http://hdl.handle.net/10498/29938Test; https://www.ijbs.com/v19p1664.pdfTest

الإتاحة: https://doi.org/10.7150/ijbs.78864Test
http://hdl.handle.net/10498/29938Test
https://www.ijbs.com/v19p1664.pdfTest

المؤلفون: Juel, Anette, Berring, Lene L, Erlangsen, Annette, Larsen, Erik R, Buus, Niels

المصدر: Juel , A , Berring , L L , Erlangsen , A , Larsen , E R & Buus , N 2024 , ' Sense of psychological ownership in co-design processes : A case study ' , Health Expectations , vol. 27 , no. 1 , e13886 . https://doi.org/10.1111/hex.13886Test

مصطلحات موضوعية: case study, ownership, participatory research, service users, website design, workshops

الوصف: INTRODUCTION: Service users are increasingly participating in health research. Although collaborative research is assumed to give users a sense of psychological ownership, little is known about the specific psychosocial processes through which ownership develops and is displayed. The present study yields insight into a process in which service users, researchers and a website designer collaborated to design a website. AIM: The aim of this study was to explore how participants developed and displayed feelings of ownership during a collaborative process to design a website. METHODS: A case study design was adopted by which audio recordings were subjected to thematic analysis and interpreted by drawing on the concept of psychological ownership. FINDINGS: A sense of psychological ownership of the website design process emerged in two distinct and overlapping phases. In the first phase, 'sense of ownership during the early design phase', only researchers and the website designer displayed a sense of ownership, which was facilitated by the research context preceding the collaborative workshops. In the second phase, 'sense of ownership during the collaborative design phase', service users gradually started to develop parallel feelings of ownership that were facilitated by workshop design activities. These activities enabled service users to increasingly control the process, to invest themselves in the process and to gain intimate knowledge of the process and its outcome. Service users' sense of ownership was displayed in their statements about the website and its elements. CONCLUSION: Participants engaged in codesign processes may develop a sense of psychological ownership at different speeds because of contextual factors. It is important to take this into account as it may complicate the formation of egalitarian work groups. PATIENT AND PUBLIC CONTRIBUTION: Parents of children with suicidal behaviour and a counsellor participated as service users in a website design process.

العلاقة: https://pure.au.dk/portal/en/publications/112b0173-fbad-4db9-b0f2-03552f1e3bfaTest

الإتاحة: https://doi.org/10.1111/hex.13886Test
https://pure.au.dk/portal/en/publications/112b0173-fbad-4db9-b0f2-03552f1e3bfaTest

المؤلفون: Smith, Amanda J., Larsen, Theodore R. B., Zimmerman, Harmony K., Virolainen, Samuel J., Meyer, Joshua J., Keranen Burden, Lisa M., Burden, Daniel L.

المساهمون: Division of Chemistry, Wheaton College

المصدر: ACS Applied Bio Materials ; volume 7, issue 3, page 1936-1946 ; ISSN 2576-6422 2576-6422

مصطلحات موضوعية: Biochemistry (medical), Biomedical Engineering, General Chemistry, Biomaterials

الإتاحة: https://doi.org/10.1021/acsabm.3c01267Test

المؤلفون: Brogaard, K., Grundahl, F., Sandquist, E. L., Slumstrup, D., Jensen, M. L., Thomsen, J. B., Jørgensen, J. H., Larsen, J. R., Bjørn, S. T., Sørensen, C. T. G., Bruntt, H., Arentoft, T., Frandsen, S., Jessen-Hansen, J., Orosz, J. A., Mathieu, R., Geller, A., Ryde, N., Stello, D., Meibom, S., Platais, I.

المصدر: A&A 649, A178 (2021)

مصطلحات موضوعية: Astrophysics - Solar and Stellar Astrophysics, Astrophysics - Astrophysics of Galaxies

الوصف: Models of stellar structure and evolution can be constrained using accurate measurements of the parameters of eclipsing binary members of open clusters. Multiple binary stars provide the means to tighten the constraints and, in turn, to improve the precision and accuracy of the age estimate of the host cluster. In the previous two papers of this series, we have demonstrated the use of measurements of multiple eclipsing binaries in the old open cluster NGC6791 to set tighter constraints on the properties of stellar models than was previously possible, thereby improving both the accuracy and precision of the cluster age. We identify and measure the properties of a non-eclipsing cluster member, V56, in NGC\,6791 and demonstrate how this provides additional model constraints that support and strengthen our previous findings. We analyse multi-epoch spectra of V56 from FLAMES in conjunction with the existing photometry and measurements of eclipsing binaries in NGC6971. The parameters of the V56 components are found to be $M_{\rm p}=1.103\pm 0.008 M_{\odot}$ and $M_{\rm s}=0.974\pm 0.007 M_{\odot}$, $R_{\rm p}=1.764\pm0.099 R_{\odot}$ and $R_{\rm s}=1.045\pm0.057 R_{\odot}$, $T_{\rm eff,p}=5447\pm125$ K and $T_{\rm eff,s}=5552\pm125$ K, and surface [Fe/H]=$+0.29\pm0.06$ assuming that they have the same abundance. The derived properties strengthen our previous best estimate of the cluster age of $8.3\pm0.3$ Gyr and the mass of stars on the lower red giant branch (RGB), which is $M_{\rm RGB} = 1.15\pm0.02M_{\odot}$ for NGC6791. These numbers therefore continue to serve as verification points for other methods of age and mass measures, such as asteroseismology.
Comment: 9 pages, 6 figures, accepted for publication in A&A

الوصول الحر: http://arxiv.org/abs/2104.14330Test

المؤلفون: Larsen, Jesper, Börjesson, Stefan, 1979, Larsen, Anders R

المصدر: Nature. 602:135-141

الوصف: The discovery of antibiotics more than 80 years ago has led to considerable improvements in human and animal health. Although antibiotic resistance in environmental bacteria is ancient, resistance in human pathogens is thought to be a modern phenomenon that is driven by the clinical use of antibiotics1. Here we show that particular lineages of methicillin-resistant Staphylococcus aureus-a notorious human pathogen-appeared in European hedgehogs in the pre-antibiotic era. Subsequently, these lineages spread within the local hedgehog populations and between hedgehogs and secondary hosts, including livestock and humans. We also demonstrate that the hedgehog dermatophyte Trichophyton erinacei produces two β-lactam antibiotics that provide a natural selective environment in which methicillin-resistant S. aureus isolates have an advantage over susceptible isolates. Together, these results suggest that methicillin resistance emerged in the pre-antibiotic era as a co-evolutionary adaptation of S. aureus to the colonization of dermatophyte-infected hedgehogs. The evolution of clinically relevant antibiotic-resistance genes in wild animals and the connectivity of natural, agricultural and human ecosystems demonstrate that the use of a One Health approach is critical for our understanding and management of antibiotic resistance, which is one of the biggest threats to global health, food security and development.

وصف الملف: print

الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-107637Test
https://doi.org/10.1038/s41586-021-04265-wTest

المؤلفون: Larsen, Simon R., Shtender, Vitalii, Hedlund, Daniel, Delczeg-Czirjak, Erna K., Beran, Premysl, Cedervall, Johan, Vishina, Alena, Hansen, Thomas C., Herper, Heike C., Svedlindh, Peter, Eriksson, Olle, 1960, Sahlberg, Martin

المصدر: eSSENCE – An eScience Collaboration Inorganic Chemistry. 61(44):17673-17681

الوصف: The atomic and magnetic structures of Mn(Co,Ge)2 are reported herein. The system crystallizes in the space group P63/mmc as a superstructure of the MgZn2-type structure. The system exhibits two magnetic transitions with associated magnetic structures, a ferromagnetic (FM) structure around room temperature, and an incommensurate structure at lower temperatures. The FM structure, occurring between 193 and 329 K, is found to be a member of the magnetic space group P63/mm'c'. The incommensurate structure found below 193 K is helical with propagation vector k = (0 0 0.0483). Crystallographic results are corroborated by magnetic measurements and ab initio calculations.

وصف الملف: print

الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-101899Test
https://doi.org/10.1021/acs.inorgchem.2c02758Test