المؤلفون: Fournié, Pierre, Acquadro, Michaël, Touboul, David, Cochener, Béatrice, Chiambaretta, Frédéric, Muraine, Marc, Borderie, Vincent, Bourges, Jean-Louis, Benmedjahed, Khadra, Tugaut, Béatrice, Bernheim, Diane, Bourcier, Tristan, Burillon, Carole, David, Thierry, Delbosc, Bernard, Gain, Philippe, Hoffart, Louis, Labetoulle, Marc, Laroche, Laurent, Malet, Florence, Orignac, Isabelle, Robert, Pierre-Yves, Thuret, Gilles, Vabres, Bertrand, Malecaze, François, Arnould, Benoit

المساهمون: Horus Pharma

المصدر: Ophthalmology and Therapy ; volume 12, issue 4, page 1939-1956 ; ISSN 2193-8245 2193-6528

مصطلحات موضوعية: Ophthalmology

الإتاحة: https://doi.org/10.1007/s40123-023-00717-wTest

المؤلفون: DESHOMMES, ELISE, BANNIER, ALICIA, LAROCHE, LAURENT, NOUR, SHOKOUFEH, PRÉVOST, MICHÈLE

المصدر: Journal (American Water Works Association), 2016 Nov 01. 108(11), E555-E570.

الوصول الحر: https://www.jstor.org/stable/90005296Test

المؤلفون: Bouheraoua, Nacim, Fouquet, Stéphane, Marcos-Almaraz, Maria Teresa, Karagogeos, Domna, Laroche, Laurent, Chédotal, Alain

المساهمون: Institut de la Vision, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO), Sorbonne Université (SU), University of Crete Heraklion (UOC)

المصدر: ISSN: 0270-6474.

مصطلحات موضوعية: Cornea, neuropilin, corneal nerves, corneal innervation, mouse, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], [SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs

الوصف: International audience ; The cornea has the densest sensory innervation of the body, originating primarily from neurons in the trigeminal ganglion. The basic principles of cornea nerve patterning have been established many years ago using classic neuroanatomical methods, such as immunocytochemistry and electrophysiology. Our understanding of the morphology and distribution of the sensory nerves in the skin has considerably progressed over the past few years through the generation and analysis of a variety of genetically modified mouse lines. Surprisingly, these lines were not used to study corneal axons. Here, we have screened a collection of transgenic and knockin mice (of both sexes) to select lines allowing the visualization and genetic manipulation of corneal nerves. We identified multiple lines, including some in which different types of corneal axons can be simultaneously observed with fluorescent proteins expressed in a combinatorial manner. We also provide the first description of the morphology and arborization of single corneal axons and identify three main types of branching pattern. We applied this genetic strategy to the analysis of corneal nerve development and plasticity. We provide direct evidence for a progressive reduction of the density of corneal innervation during aging. We also show that the semaphorin receptor neuropilin-1 acts cell-autonomously to control the development of corneal axons and that early axon guidance defects have long-term consequences on corneal innervation.

العلاقة: hal-02309381; https://hal.sorbonne-universite.fr/hal-02309381Test; https://hal.sorbonne-universite.fr/hal-02309381/documentTest; https://hal.sorbonne-universite.fr/hal-02309381/file/Bouheraoua%20et%20al.%20-%202019%20-%20Genetic%20Analysis%20of%20the%20Organization,%20Development,.pdfTest

الإتاحة: https://doi.org/10.1523/JNEUROSCI.1401-18.2018Test
https://hal.sorbonne-universite.fr/hal-02309381Test
https://hal.sorbonne-universite.fr/hal-02309381/documentTest
https://hal.sorbonne-universite.fr/hal-02309381/file/Bouheraoua%20et%20al.%20-%202019%20-%20Genetic%20Analysis%20of%20the%20Organization,%20Development,.pdfTest

المؤلفون: Ouanezar, Sofiane, Sandali, Otman, Atia, Raphael, Temstet, Cyrille, Georgeon, Cristina, Laroche, Laurent, Borderie, Vincent, Bouheraoua, Nacim

المساهمون: Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO), Sorbonne Université (SU), Institut de la Vision, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0886-3350.

مصطلحات موضوعية: [SDV]Life Sciences [q-bio]

الوصف: International audience

العلاقة: hal-03486073; https://hal.science/hal-03486073Test; https://hal.science/hal-03486073/documentTest; https://hal.science/hal-03486073/file/S0886335018308666.pdfTest; PII: S0886-3350(18)30866-6

الإتاحة: https://doi.org/10.1016/j.jcrs.2018.09.024Test
https://hal.science/hal-03486073Test
https://hal.science/hal-03486073/documentTest
https://hal.science/hal-03486073/file/S0886335018308666.pdfTest

المؤلفون: Rouimi, Fabien, Ouanezar, Sofiane, Goemaere, Isabelle, Bayle, Anne Charlotte, Borderie, Vincent, Laroche, Laurent, Bouheraoua, Nacim

المساهمون: Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO), Sorbonne Université (SU), Institut de la Vision, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0886-3350.

مصطلحات موضوعية: [SDV]Life Sciences [q-bio]

الوصف: International audience

العلاقة: hal-03487913; https://hal.science/hal-03487913Test; https://hal.science/hal-03487913/documentTest; https://hal.science/hal-03487913/file/S0886335019301725.pdfTest; PII: S0886-3350(19)30172-5

الإتاحة: https://doi.org/10.1016/j.jcrs.2019.02.039Test
https://hal.science/hal-03487913Test
https://hal.science/hal-03487913/documentTest
https://hal.science/hal-03487913/file/S0886335019301725.pdfTest

مستخلص: The present invention relates to a process for manufacturing an object made of biocompatible hydrogel by molding a polymer solution in a mold made of a particular material, said process comprising the following steps: (i) preparation of a polymer solution by dissolving a copolymer of acrylonitrile and of an olefinically unsaturated co-monomer bearing anionic groups in an aprotic solvent, optionally in the presence of a nonsolvent, (ii) shaping and the start of gelation of the polymer solution obtained at the end of step (i) in a mold consisting of a material containing said nonsolvent or of a material permeable to said nonsolvent, (iii) immersion of the object undergoing gelation resulting from step (ii) in a nonsolvent. The present invention also relates to the objects made of biocompatible hydrogel resulting from this process such as, for example, intracorneal lenses (or lenticules) implantable in the cornea or any other implants usable in ophthalmology.

المؤلفون: Bouheraoua, Nacim, Poirel, Laurent, Bourreau, Baptiste, Bonnin, Rémy, Laroche, Laurent, Naas, Thierry, Nordmann, Patrice

المساهمون: University Paris XI/INSERM

المصدر: Antimicrobial Agents and Chemotherapy ; volume 62, issue 5 ; ISSN 0066-4804 1098-6596

الوصف: Integrons are genetic elements that can acquire and rearrange gene cassettes. The bla BEL-1 gene encodes an extended-spectrum β-lactamase, BEL-1, that is present at the second position of the variable region of class 1 integrons identified in Pseudomonas aeruginosa . The mobility of the bel-1 gene cassette was analyzed under physiological conditions and with the integrase gene being overexpressed. Cassette mobility in Escherichia coli was detected by excision/integration into the recipient integron In 3 on the conjugative plasmid R388 with the overproduced integrase. Despite several antibiotic pressures, the bel-1 cassette remained at the second position in the integron, highlighting its stability in P. aeruginosa . Overexpression of the integrase gene in E. coli induced bel-1 cassette recombination. However, cassettes containing two genes ( bla BEL-1 and smr2 or bla BEL-1 and aacA4 ) were excised, suggesting that the bel-1 cassette attC site was defective. We show that bel-1 is a stable gene cassette under physiological growth conditions, irrespective of the selective antibiotic pressure, that may be mobilized upon overexpression of the integrase gene.

الإتاحة: https://doi.org/10.1128/aac.00030-18Test

المؤلفون: Levy, Ora, Labbé, Antoine, Borderie, Vincent, Hamiche, Taous, Dupas, Bénédicte, Laroche, Laurent, Baudouin, Christophe, Bouheraoua, Nacim

المصدر: Clinical & Experimental Ophthalmology ; volume 45, issue 5, page 455-463 ; ISSN 1442-6404 1442-9071

الوصف: Background To evaluate quantitative and qualitative changes in sub‐basal corneal nerves (SBN) via in vivo confocal microscopy in patients with Sjögren syndrome dry eye (SSDE) treated with topical cyclosporine A (CsA). Design Prospective, observational, non‐randomized study. Participants Thirty eyes of 30 patients with SSDE refractory to conventional treatment treated with CsA 0.05% twice daily for 6 months. Fifteen eyes of 15 healthy, age and gender matched, volunteers constituted the control group at baseline. Methods A clinical evaluation of dry eye, corneal sensation using Cochet–Bonnet esthesiometry and in vivo confocal microscopy analysis of the central cornea were performed prospectively at baseline for all patients, and after 6 months of treatment with CsA. Main outcome measures Density, number, reflectivity and tortuosity of SBN, dendritic cell (DC) density, esthesiometry, and dry eye signs and symptoms. Results Topical CsA 0.05% improved clinical signs and symptoms, and increased corneal sensitivity. Following treatment, SBN density was significantly increased ( P < 0.0001) associated with a decreased in DC density ( P < 0.0001). The increase in SBN density after treatment was positively correlated with baseline SBN density ( R 2 = 0.33; P = 0.0008) and negatively correlated with baseline Ocular Surface Disease Index ( R 2 = 0.28; P = 0.002), Oxford score ( R 2 = 0.31; P = 0.002), and DC density ( R 2 = 0.37; P = 0.0003). Conclusions Topical CsA led to an increase in corneal SBN density, improving clinical signs and symptoms of SSDE. Our results also suggest an improved response to treatment in patients with less initial nerve damage.

الإتاحة: https://doi.org/10.1111/ceo.12898Test

المؤلفون: Borderie, Marie, Grieve, Kate, Irsch, Kristina, Ghoubay, Djida, Georgeon, Cristina, De Sousa, Celine, Laroche, Laurent, Borderie, Vincent M.

المساهمون: Agence Nationale de la Recherche, European Research Council

المصدر: Acta Ophthalmologica ; volume 95, issue 4 ; ISSN 1755-375X 1755-3768

الوصف: Purpose To provide quantitative parameters for assessment of human donor corneal stroma by imaging stromal features of diseased and normal human corneas with full‐field optical coherence microscopy (FFOCM), using confocal microscopy (CM) and histology as reference techniques. Methods Bowman's layer (BL) thickness and keratocyte density were assessed ex vivo in 23 human donor corneas and 27 human pathological corneas (keratoconus and other corneal disorders) with FFOCM, CM and histology. Stromal backscattering was assessed with FFOCM. Additionally, 10 normal human corneas were assessed in vivo with CM. Results In FFOCM, the logarithm of the normalized stromal reflectivity was a linear function of stromal depth ( R 2 = 0.95) in human donor corneas. Compared with keratoconus corneas, human donor corneas featured higher BL thickness (p = 0.0014) with lower coefficient of variation (BL‐COV; p = 0.0002), and linear logarithmic stromal reflectivity with depth (higher R 2 , p = 0.0001). Compared with other corneal disorders, human donor corneas featured lower BL‐COV (p = 0.012) and higher R 2 (p = 0.0001). Using the 95% confidence limits of the human donor cornea group, BL thickness < 6.5 μ m (sensitivity, 57%; specificity, 100%), BL‐COV > 18.6% (79%; 100%) and R 2 < 0.94 (93%; 71%) were revealed as indictors of abnormal cornea. In CM, keratocyte density decreased with stromal depth ( r = −0.56). The mean overall keratocyte density (cells/mm 2 ) was 205 in human donor corneas, 244 in keratoconus, 176 in other corneal disorders and 386 in normal corneas. Conclusion Full‐field optical coherence microscopy (FFOCM) provides precise and reliable parameters for non‐invasive assessment of human donor corneal stroma during storage, enabling detection of stromal disorders that could impair the results of keratoplasty.

الإتاحة: https://doi.org/10.1111/aos.13351Test

المؤلفون: CARTIER, CLÉMENT, LAROCHE, LAURENT, DESHOMMES, ELISE, NOUR, SHOKOUFEH, RICHARD, GUILLAUME, EDWARDS, MARC, PRÉVOST, MICHÈLE

المصدر: Journal (American Water Works Association), 2011 Mar 01. 103(3), 55-67.

الوصول الحر: https://www.jstor.org/stable/41314443Test