يعرض 1 - 7 نتائج من 7 نتيجة بحث عن '"Kszuk-Jendrysik, M."', وقت الاستعلام: 0.69s تنقيح النتائج
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    المصدر: Microscopy and Microanalysis ; volume 25, issue 4, page 1004-1016 ; ISSN 1431-9276 1435-8115

    مصطلحات موضوعية: Instrumentation

    الوصف: The process of autophagy has been detected in the midgut epithelium of four millipede species: Julus scandinavius , Polyxenus lagurus , Archispirostreptus gigas , and Telodeinopus aoutii. It has been examined using transmission electron microscopy (TEM), which enabled differentiation of cells in the midgut epithelium, and some histochemical methods (light microscope and fluorescence microscope). While autophagy appeared in the cytoplasm of digestive, secretory, and regenerative cells in J. scandinavius and A. gigas , in the two other species, T. aoutii and P. lagurus , it was only detected in the digestive cells. Both types of macroautophagy, the selective and nonselective processes, are described using TEM. Phagophore formation appeared as the first step of autophagy. After its blind ends fusion, the autophagosomes were formed. The autophagosomes fused with lysosomes and were transformed into autolysosomes. As the final step of autophagy, the residual bodies were detected. Autophagic structures can be removed from the midgut epithelium via, e.g., atypical exocytosis. Additionally, in P. lagurus and J. scandinavius , it was observed as the neutralization of pathogens such as Rickettsia -like microorganisms. Autophagy and apoptosis ca be analyzed using TEM, while specific histochemical methods may confirm it.

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    المؤلفون: Rost-Roszkowska, M. M.1 magdalena.rost-roszkowska@us.edu.pl, Kszuk-Jendrysik, M.1, Marchewka, A.1, Poprawa, I.1

    المصدر: Protoplasma. Jan2018, Vol. 255 Issue 1, p43-55. 13p.

    مصطلحات موضوعية: *MILLIPEDES, *EPITHELIAL cells, *LIVER cells, *REGENERATION (Biology), *MITOSIS, *PLANTS

    مستخلص: The midgut of millipedes is composed of a simple epithelium that rests on a basal lamina, which is surrounded by visceral muscles and hepatic cells. As the material for our studies, we chose Telodeinopus aoutii (Demange, 1971) (Kenyan millipede) (Diplopoda, Spirostreptida), which lives in the rain forests of Central Africa. This commonly reared species is easy to obtain from local breeders and easy to culture in the laboratory. During our studies, we used transmission and scanning electron microscopes and light and fluorescent microscopes. The midgut epithelium of the species examined here shares similarities to the structure of the millipedes analyzed to date. The midgut epithelium is composed of three types of cells-digestive, secretory, and regenerative cells. Evidence of three types of secretion have been observed in the midgut epithelium: merocrine, apocrine, and microapocrine secretion. The regenerative cells of the midgut epithelium in millipedes fulfill the role of midgut stem cells because of their main functions: self-renewal (the ability to divide mitotically and to maintain in an undifferentiated state) and potency (ability to differentiate into digestive cells). We also confirmed that spot desmosomes are common intercellular junctions between the regenerative and digestive cells in millipedes. [ABSTRACT FROM AUTHOR]