المؤلفون: Getsy, Paulina M., Coffee, Gregory A., Kelley, Thomas J., Lewis, Stephen J.

المصدر: Frontiers in Physiology ; volume 14 ; ISSN 1664-042X

مصطلحات موضوعية: Physiology (medical), Physiology

الوصف: Histone deacetylase 6 (HDAC6) is a class II histone deacetylase that is predominantly localized in the cytoplasm of cells. HDAC6 associates with microtubules and regulates acetylation of tubulin and other proteins. The possibility that HDAC6 participates in hypoxic signaling is supported by evidence that 1) hypoxic gas challenges cause microtubule depolymerization, 2) expression of hypoxia inducible factor alpha (HIF-1α) is regulated by microtubule alterations in response to hypoxia, and 3) inhibition of HDAC6 prevents HIF-1α expression and protects tissue from hypoxic/ischemic insults. The aim of this study was to address whether the absence of HDAC6 alters ventilatory responses during and/or after hypoxic gas challenge (10% O 2 , 90% N 2 for 15 min) in adult male wildtype (WT) C57BL/6 mice and HDAC6 knock-out (KO) mice. Key findings were that 1) baseline values for frequency of breathing, tidal volume, inspiratory and expiratory times, and end expiratory pause were different between knock-out mice and wildtype mice, 2) ventilatory responses during hypoxic challenge were more robust in KO mice than WT mice for recorded parameters including, frequency of breathing, minute ventilation, inspiratory and expiratory durations, peak inspiratory and expiratory flows, and inspiratory and expiratory drives, and 3) responses upon return to room-air were markedly different in KO compared to WT mice for frequency of breathing, minute ventilation, inspiratory and expiratory durations, end expiratory pause (but not end inspiratory pause), peak inspiratory and expiratory flows, and inspiratory and expiratory drives. These data suggest that HDAC6 may have a fundamentally important role in regulating the hypoxic ventilatory response in mice.

الإتاحة: https://doi.org/10.3389/fphys.2023.1332810Test

مستخلص: A beach umbrella anchoring system including an anchor member container, in the form of a cylindrical beach umbrella carrying bag, for containing a weighting medium, such as sand or water, and a connector for connecting the container to a beach umbrella restraining device; and a beach umbrella restraining device including a flexible cable member, a releasable connector fixed to one end of the cable for releasably connecting the cable to a beach umbrella, a second releasable connector, fixed to the other end of the cable, for releasably connecting the cable member to the anchor member, and a third releasable connector, attached to the anchor member for releasably attaching to the ground. The anchoring system may be supplied in the form of a kit comprising the anchoring member (beach bag), umbrella and the restraining device.

المؤلفون: Maria A. Sierra, Krista A. Ryon, Braden T. Tierney, Jonathan Foox, Chandrima Bhattacharya, Evan Afshin, Daniel Butler, Stefan J. Green, W. Kelley Thomas, Jordan Ramsdell, Nathan J. Bivens, Ken McGrath, Christopher E. Mason, Scott W. Tighe

المصدر: Environmental Microbiome, Vol 17, Iss 1, Pp 1-20 (2022)

مصطلحات موضوعية: Hillier Lake, Hypersaline lake, Polyextremophile, Pigments, Microbiome, Metagenomics, Environmental sciences, GE1-350, Microbiology, QR1-502

الوصف: Abstract Lake Hillier is a hypersaline lake known for its distinctive bright pink color. The cause of this phenomenon in other hypersaline sites has been attributed to halophiles, Dunaliella, and Salinibacter, however, a systematic analysis of the microbial communities, their functional features, and the prevalence of pigment-producing-metabolisms has not been previously studied. Through metagenomic sequencing and culture-based approaches, our results evidence that Lake Hillier is composed of a diverse set of microorganisms including archaea, bacteria, algae, and viruses. Our data indicate that the microbiome in Lake Hillier is composed of multiple pigment-producer microbes, including Dunaliella, Salinibacter, Halobacillus, Psychroflexus, Halorubrum, many of which are cataloged as polyextremophiles. Additionally, we estimated the diversity of metabolic pathways in the lake and determined that many of these are related to pigment production. We reconstructed complete or partial genomes for 21 discrete bacteria (N = 14) and archaea (N = 7), only 2 of which could be taxonomically annotated to previously observed species. Our findings provide the first metagenomic study to decipher the source of the pink color of Australia’s Lake Hillier. The study of this pink hypersaline environment is evidence of a microbial consortium of pigment producers, a repertoire of polyextremophiles, a core microbiome and potentially novel species.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2524-6372Test

الوصول الحر: https://doaj.org/article/816e8e02963f4d6c85b32181798eaa3cTest

المؤلفون: Stephany Barrera, Sonia Vázquez-Flores, David Needle, Nadia Rodríguez-Medina, Dianella Iglesias, Joseph L. Sevigny, Lawrence M. Gordon, Stephen Simpson, W. Kelley Thomas, Hectorina Rodulfo, Marcos De Donato

المصدر: Antibiotics, Vol 12, Iss 12, p 1662 (2023)

مصطلحات موضوعية: Salmonella, dairy farms, periparturient cows, calves, maternity beds, virulence genes, Therapeutics. Pharmacology, RM1-950

الوصف: Salmonella isolated from dairy farms has a significant effect on animal health and productivity. Different serogroups of Salmonella affect both human and bovine cattle causing illness in both reservoirs. Dairy cows and calves can be silent Salmonella shedders, increasing the possibility of dispensing Salmonella within the farm. The aim of this study was to determine the genomic characteristics of Salmonella isolates from dairy farms and to detect the presence of virulence and antimicrobial resistance genes. A total of 377 samples were collected in a cross-sectional study from calves, periparturient cow feces, and maternity beds in 55 dairy farms from the states of Aguascalientes, Baja California, Chihuahua, Coahuila, Durango, Mexico, Guanajuato, Hidalgo, Jalisco, Queretaro, San Luis Potosi, Tlaxcala, and Zacatecas. Twenty Salmonella isolates were selected as representative strains for whole genome sequencing. The serological classification of the strains was able to assign groups to only 12 isolates, but with only 5 of those being consistent with the genomic serotyping. The most prevalent serovar was Salmonella Montevideo followed by Salmonella Meleagridis. All isolates presented the chromosomal aac(6′)-Iaa gene that confers resistance to aminoglycosides. The antibiotic resistance genes qnrB19, qnrA1, sul2, aph(6)-Id, aph(3)-ld, dfrA1, tetA, tetC, flor2, sul1_15, mph(A), aadA2, blaCARB, and qacE were identified. Ten pathogenicity islands were identified, and the most prevalent plasmid was Col(pHAD28). The main source of Salmonella enterica is the maternity areas, where periparturient shedders are contaminants and perpetuate the pathogen within the dairy in manure, sand, and concrete surfaces. This study demonstrated the necessity of implementing One Health control actions to diminish the prevalence of antimicrobial resistant and virulent pathogens including Salmonella.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2079-6382/12/12/1662Test; https://doaj.org/toc/2079-6382Test

الوصول الحر: https://doaj.org/article/e1037e9480fb4a2e871a75807a3fa283Test

المؤلفون: Brandy Moser, Dustin Moore, Bishnu Khadka, Carrie Lyons, Tom Foxall, Cheryl P. Andam, Cooper J. Parker, Chinedu Ochin, Mahdi Garelnabi, Joseph Sevigny, W. Kelley Thomas, Sherman Bigornia, Maria Carlota Dao

المصدر: Frontiers in Nutrition, Vol 9 (2023)

مصطلحات موضوعية: type 2 diabetes, gastrointestinal microbiome, inflammation, metabolic endotoxemia, Bhutanese refugee adults, Nutrition. Foods and food supply, TX341-641

الوصف: IntroductionSouth Asian refugees experience a high risk of obesity and diabetes yet are often underrepresented in studies on chronic diseases and their risk factors. The gut microbiota and gut permeability, as assessed through circulating lipopolysaccharide binding protein (LBP), may underlie the link between chronic inflammation and type 2 diabetes (T2D). The composition of the gut microbiota varies according to multiple factors including demographics, migration, and dietary patterns, particularly fiber intake. However, there is no evidence on the composition of the gut microbiota and its relationship with metabolic health in refugee populations, including those migrating to the United States from Bhutan. The objective of this study was to examine glycemic status in relation to LBP, systemic inflammation fiber intake, and gut microbiota composition in Bhutanese refugee adults residing in New Hampshire (n = 50).MethodsThis cross-sectional study included a convenience sample of Bhutanese refugee adults (N = 50) in NH. Established bioinformatics pipelines for metagenomic analysis were used to determine relative genus abundance, species richness, and alpha diversity measures from shallow shotgun sequences. The relationships between inflammatory markers, gut microbiota composition, dietary fiber, and glycemic status were analyzed.ResultsWe identified a substantial chronic disease burden in this study population, and observed a correlation between glycemic status, LBP, and inflammation, and a correlation between glycemic status and gut microbiome alpha diversity. Further, we identified a significant correlation between proinflammatory taxa and inflammatory cytokines. SCFA-producing taxa were found to be inversely correlated with age.ConclusionTo date, this is the most comprehensive examination of metabolic health and the gut microbiome in a Bhutanese refugee population in NH. The findings highlight areas for future investigations of inflammation and glycemic impairment, in addition to informing potential interventions targeting this vulnerable population.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fnut.2022.1059163/fullTest; https://doaj.org/toc/2296-861XTest

الوصول الحر: https://doaj.org/article/a49d6f52456a418dab8ba253b36eac1dTest

المؤلفون: Endres, Tori, Duesler, Lori, Corey, Deborah A., Kelley, Thomas J.

المساهمون: Cystic Fibrosis Foundation

المصدر: Scientific Reports ; volume 13, issue 1 ; ISSN 2045-2322

مصطلحات موضوعية: Multidisciplinary

الوصف: Microtubule dysfunction has been implicated as a mediator of inflammation in multiple diseases such as disorders of the cardiovascular and neurologic systems. Tubulin polymerization promoting protein (Tppp) facilitates microtubule elongation and regulates tubulin acetylation through inhibition of cytosolic deacetylase enzymes. Pathologic alterations in microtubule structure and dynamics have been described in cystic fibrosis (CF) and associated with inflammation, however the causality and mechanism remain unclear. Likewise, Tppp has been identified as a potential modifier of CF airway disease severity. Here we directly assess the impact of microtubule dysfunction on infection and inflammation by interrogating wild type and a Tppp knockout mouse model ( Tppp − / − ). Mice are challenged with a clinical isolate of Pseudomonas aeruginosa -laden agarose beads and assessed for bacterial clearance and inflammatory markers. Tppp − / − mouse model demonstrate impaired bacterial clearance and an elevated inflammatory response compared to control mice. These data are consistent with the hypothesis microtubule dysregulation is sufficient to lead to CF-like airway responses in mice.

الإتاحة: https://doi.org/10.1038/s41598-023-39443-5Test
https://www.nature.com/articles/s41598-023-39443-5.pdfTest
https://www.nature.com/articles/s41598-023-39443-5Test

المؤلفون: Schelde, Karen, Rosenjack, Julie, Sonneborn, Claire, Jafri, Anjum, Kavran, Michael, Brumbaugh, Sarah, Rietsch, Arne, Darrah, Rebecca J, Hodges, Craig A, Flask, Christopher A, Kelley, Thomas J, Drumm, Mitchell L

المساهمون: The Doris and Floyd Kimble Foundation and MPB Charitable Foundation, Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland Digestive Diseases Research Core Center, Carlsbergfondet, Cleveland Clinical and Translational Science Collaborative, The Research Center for Children's Health, Cystic Fibrosis Foundation

المصدر: Laboratory Animals ; volume 57, issue 6, page 611-622 ; ISSN 0023-6772 1758-1117

مصطلحات موضوعية: General Veterinary, Animal Science and Zoology

الوصف: The laboratory mouse is used extensively for human disease modeling and preclinical therapeutic testing for efficacy, biodistribution, and toxicity. The variety of murine models available, and the ability to create new ones, eclipses all other species, but the size of mice and their organs create challenges for many in vivo studies. For pulmonary research, improved methods to access murine airways and lungs, and track substances administered to them, would be desirable. A nonsurgical endoscopic system with a camera, effectively a bronchoscope, coupled with a cryoimaging fluorescence microscopy technique to view the lungs in 3D, is described here that allows visualization of the procedure, including the anatomical location at which substances are instilled and fluorescence detection of those substances. We have applied it to bacterial infection studies to characterize better and optimize a chronic lung infection murine model in which we instill bacteria-laden agarose beads into the airways and lungs to extend the duration of the infection and inflammation. The use of the endoscope as guidance for placing a catheter into the airways is simple and quick, requiring only momentary sedation, and reduces post-procedural mortality compared with our previous instillation method that includes a trans-tracheal surgery. The endoscopic method improves speed and precision of delivery while reducing the stress on animals and the number of animals generated and used for experiments.

الإتاحة: https://doi.org/10.1177/00236772231175553Test

المؤلفون: Norman, Carrie, Kelley, Thomas

المصدر: Log, 2021 Jan 01(51), 19-27.

الوصول الحر: https://www.jstor.org/stable/27092899Test

المؤلفون: Claudia Husseneder, Patrick Rayle, Benjamin G. Aker, W. Kelley Thomas, Lane D. Foil

المصدر: Frontiers in Environmental Science, Vol 10 (2022)

مصطلحات موضوعية: 18S metabarcoding, next-generation sequencing, environmental DNA, meiofauna, soil organisms, Environmental sciences, GE1-350

الوصف: Tidal marshes are among the most valuable, productive, and vulnerable ecosystems with high biodiversity. Louisiana’s saltmarshes are endangered by natural and man-made stressors, including oil pollution, saltwater intrusion, and land loss due to sea level rise and erosion. Freshwater diversions have been planned to restore sediment input from the Mississippi River to rebuild marsh habitats in South Louisiana. These proposed diversions will undoubtedly change salinity levels, which is a major controlling factor in the distribution of marsh organisms, including those in soil; however, detailed pre-event inventories are lacking. This study describes the diversity of metazoan meiofauna (organisms between 45 and 500 μm) and environmental DNA in marsh soil collected in 2018 from Barataria and Caillou Bay, Louisiana, across three salinity zones and four distances from the marsh edge. Diversity analyses using 18S rRNA gene metabarcodes identified salinity as a factor impacting soil metazoan composition. Nematoda and Mollusca were equally distributed across salinity zones. Gastrotricha, Bryozoa, Rotifera, and Platyhelminthes were more prevalent in low salinity while Kinorhyncha were not detected in low salinity. Annelida and insects were equally common in low and high salinity but less in mid salinity. Five nematodes (Eumonhystera filiformis, two Prismatolaimus spp., Anoplostoma sp., and Prodorylaimus sp.), two annelids (Marionina southerni and Dendronereis aestuarina), two platyhelminthes (Rhynchoscolex simplex and Olisthanella truncula), the gastrotrich Chaetonotus novenarius and four collembola and ostracods appear to be low salinity bioindicators and are expected to expand range with freshwater diversions. No frequently detected organisms were unique to mid or high salinity zones, but four Nematoda (Meleidogyne spartinae, Prochaetosoma sp., Halalaimus sp., and Dichromadora sp.), two Annelida (Alitta succinea and Namalycastis jaya), two Platyhelminthes (Macrostomum kepneri and Mesorhynchus terminostylis), and one Kinorhyncha (Echinoderes sp.) were never detected in low salinity zones. None of the frequently detected taxa were unique for a particular distance from the marsh edge or bay. This dataset will be useful as baseline for assessing how soil communities will change in response to salinity changes caused by freshwater diversions and saltwater intrusion as well as measuring the environmental impact of pollution and other stressors.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fenvs.2022.950527/fullTest; https://doaj.org/toc/2296-665XTest

الوصول الحر: https://doaj.org/article/7305a99853e546e6bb43b2e8b53d6c64Test

المؤلفون: Kelley, Thomas, Kessel, Anthony, Collings, Rosalyn, Rubenstein, Brian, Monnickendam, Charlotte, Solomon, Andrew

المصدر: Journal of Public Mental Health, 2021, Vol. 20, Issue 1, pp. 43-50.

الوصول الحر: http://www.emeraldinsight.com/doi/10.1108/JPMH-03-2020-0019Test