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1دورية أكاديمية
المؤلفون: Ali A. Al-Mubarak, George Markousis Mavrogenis, Xuanxuan Guo, Marco De Bruyn, Mintu Nath, Simon P.R. Romaine, Niels Grote Beverborg, Karla Arevalo Gomez, Sietske N. Zijlstra, Dirk J. van Veldhuisen, Nilesh J. Samani, Adriaan A. Voors, Peter van der Meer, Nils Bomer
المصدر: Redox Biology, Vol 70, Iss , Pp 103046- (2024)
مصطلحات موضوعية: Selenium, Heart failure, Inflammation, Micronutrients, T cells, Medicine (General), R5-920, Biology (General), QH301-705.5
الوصف: Background: Low selenium concentrations are associated with worse outcomes in heart failure (HF). However, the underlying pathophysiologic mechanisms remain incompletely understood. Therefore, we aimed to contrast serum selenium concentrations to blood biomarker and transcriptomic profiles in patients with HF. Methods: Circulating biomarkers, whole blood transcriptomics and serum selenium measurements in a cohort of 2328 patients with HF were utilized. Penalized linear regression and gene expression analysis were used to assess biomarker and transcriptomics profiles, respectively. As a proof-of-principle, potential causal effects of selenium on excreted cytokines concentrations were investigated using human peripheral blood mononuclear cells (PBMCs). Results: Mean selenium levels were 60.6 μg/L in Q1 and 122.0 μg/L in Q4. From 356 biomarkers and 20 clinical features, the penalized linear regression model yielded 44 variables with
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S2213231724000223Test; https://doaj.org/toc/2213-2317Test
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2دورية أكاديمية
المؤلفون: Dennis M. L. W. Kruk, Marissa Wisman, Jacobien A. Noordhoek, Mehmet Nizamoglu, Marnix R. Jonker, Harold G. de Bruin, Karla Arevalo Gomez, Nick H. T. ten Hacken, Simon D. Pouwels, Irene H. Heijink
المصدر: Cells, Vol 10, Iss 11, p 2860 (2021)
مصطلحات موضوعية: COPD, emphysema, cell therapy, lung repair, MSCs, alveolar epithelium, Cytology, QH573-671
الوصف: COPD is characterized by irreversible lung tissue damage. We hypothesized that lung-derived mesenchymal stromal cells (LMSCs) reduce alveolar epithelial damage via paracrine processes, and may thus be suitable for cell-based strategies in COPD. We aimed to assess whether COPD-derived LMSCs display abnormalities. LMSCs were isolated from lung tissue of severe COPD patients and non-COPD controls. Effects of LMSC conditioned-medium (CM) on H2O2-induced, electric field- and scratch-injury were studied in A549 and NCI-H441 epithelial cells. In organoid models, LMSCs were co-cultured with NCI-H441 or primary lung cells. Organoid number, size and expression of alveolar type II markers were assessed. Pre-treatment with LMSC-CM significantly attenuated oxidative stress-induced necrosis and accelerated wound repair in A549. Co-culture with LMSCs supported organoid formation in NCI-H441 and primary epithelial cells, resulting in significantly larger organoids with lower type II-marker positivity in the presence of COPD-derived versus control LMSCs. Similar abnormalities developed in organoids from COPD compared to control-derived lung cells, with significantly larger organoids. Collectively, this indicates that LMSCs’ secretome attenuates alveolar epithelial injury and supports epithelial repair. Additionally, LMSCs promote generation of alveolar organoids, with abnormalities in the supportive effects of COPD-derived LMCS, reflective of impaired regenerative responses of COPD distal lung cells.
وصف الملف: electronic resource
النص الكامل