المؤلفون: Mengjie Li, Jun Tian, Kangkang Yu, He Liu, Xiaoqi Yu, Nan Wang, Qimei Gong, Kun Li, Ya Shen, Xi Wei

المصدر: Bioactive Materials, Vol 36, Iss , Pp 524-540 (2024)

مصطلحات موضوعية: Small extracellular vesicle, Dental follicle stem cell, Pulpitis, Oxidative stress, ROS-Responsive hydrogel, Materials of engineering and construction. Mechanics of materials, TA401-492, Biology (General), QH301-705.5

الوصف: Pulpitis, an inflammatory disease of dental pulp tissues, ultimately results in the loss of pulp defense properties. Existing clinical modalities cannot effectively promote inflamed pulp repair. Oxidative stress is a major obstacle inhibiting pulp repair. Due to their powerful antioxidative capacity, mesenchymal stem cell-derived small extracellular vesicles (MSC-sEVs) exhibit potential for treating oxidative stress-related disorders. However, whether MSC-sEVs shield dental pulp tissues from oxidative damage is largely unknown. Here, we showed that dental follicle stem cell-derived sEVs (DFSC-sEVs) have antioxidative and prohealing effects on a rat LPS-induced pulpitis model by enhancing the survival, proliferation and odontogenesis of H2O2-injured dental pulp stem cells (DPSCs). Additionally, DFSC-sEVs restored the oxidative/antioxidative balance in DPSC mitochondria and had comparable effects on ameliorating mitochondrial dysfunction with the mitochondrion-targeted antioxidant Mito-Tempo. To improve the efficacy of DFSC-sEVs, we fabricated an intelligent and injectable hydrogel to release DFSC-sEVs by combining sodium alginate (SA) and the ROS sensor RhB-AC. The newly formed SA-RhB hydrogel efficiently encapsulates DFSC-sEVs and exhibits controlled release of DFSC-sEVs in a HClO/ClO− concentration-dependent manner, providing a synergistic antioxidant effect with DFSC-sEVs. These results suggest that DFSC-sEVs-loaded SA-RhB is a promising minimally invasive treatment for pulpitis by enhancing tissue repair in the pulp wound microenvironment.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2452199X24002597Test; https://doaj.org/toc/2452-199XTest

الوصول الحر: https://doaj.org/article/f09a5cf29d4744788f3e3967ef076f35Test

المؤلفون: Jia Zhang, Chunbao Li, Mingyang An, Yidong Wu, Kangkang Yu, Yujie Liu, The Youth Group of the Sports Medicine Branch of the Chinese Medical Association, The Hip Sports Injury Working Committee of the Sports Medicine Branch of the Chinese Medical Association, The Hip Preservation Group of the Rehabilitation, Reconstruction Surgery Committee of the Chinese Association of Rehabilitation Medicine, The Hip Preservation Group of Orthopaedic Surgeon Branch of the Chinese Medical Doctor Association, Yanjie Yin

المصدر: Chinese Medical Journal, Vol 136, Iss 9, Pp 1012-1014 (2023)

مصطلحات موضوعية: Medicine

وصف الملف: electronic resource

العلاقة: http://journals.lww.com/10.1097/CM9.0000000000002483Test; https://doaj.org/toc/0366-6999Test; https://doaj.org/toc/2542-5641Test

الوصول الحر: https://doaj.org/article/56161aba766a43c78fac6e1cfa609699Test

المؤلفون: Ning Li, Kangkang Yu, Minhui Dong, Jinyu Wang, Feifei Yang, Haoxiang Zhu, Jie Yu, Jingshu Yang, Wentao Xie, Bidisha Mitra, Richeng Mao, Feizhen Wu, Haitao Guo, Jiming Zhang

المصدر: Emerging Microbes and Infections, Vol 11, Iss 1, Pp 1876-1889 (2022)

مصطلحات موضوعية: Chronic hepatitis B, interferon therapy, liver biopsy, transcriptome, HBeAg, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502

الوصف: Chronic hepatitis B virus (HBV) infection remains a substantial public health burden worldwide. Alpha-interferon (IFNα) is one of the two currently approved therapies for chronic hepatitis B (CHB), to explore the mechanisms underlying IFNα treatment response, we investigated baseline and 24-week on-treatment intrahepatic gene expression profiles in 21 CHB patients by mRNA-seq. The data analyses demonstrated that PegIFNα treatment significantly induced antiviral responses. Responders who achieved HBV DNA loss and HBeAg or HBsAg seroconversion displayed higher fold change and larger number of up-regulated interferon-stimulated genes (ISGs). Interestingly, lower expression levels of certain ISGs were observed in responders in their baseline biopsy samples. In HBeAg+ patients, non-responders had relative higher baseline HBeAg levels than responders. More importantly, HBeAg− patients showed higher HBsAg loss rate than HBeAg+ patients. Although a greater fold change of ISGs was observed in HBeAg− patients than HBeAg+ patients, upregulation of ISGs in HBeAg+ responders exceeded HBeAg− responders. Notably, PegIFNα treatment increased monocyte and mast cell infiltration, but decreased CD8 T cell and M1 macrophage infiltration in both responders and non-responders, while B cell infiltration was increased only in responders. Moreover, co-expression analysis identified ribosomal proteins as critical players in antiviral response. The data also indicate that IFNα may influence the production of viral antigens associated with endoplasmic reticulum. Collectively, the intrahepatic transcriptome analyses in this study enriched our understanding of IFN-mediated antiviral effects in CHB patients and provided novel insights into the development of potential strategies to improve IFNα therapy.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2222-1751Test

الوصول الحر: https://doaj.org/article/34ac08a2fed74782886862be48e5288dTest

المؤلفون: Jiaming Tian, Xinghua He, Hanzhi Wu, Yan Chen, Kangkang Yu, Mingli Zhang, Yanhua Wang

المصدر: Ecological Indicators, Vol 147, Iss , Pp 109976- (2023)

مصطلحات موضوعية: River-lake ecotone, Poyang Lake, Lipid biomarker, Source apportionment, Driving force, Ecology, QH540-549.5

الوصف: Carbon molecular deposition in sediments removes organic carbon (OC) from the biosphere-atmosphere carbon cycle and therefore limits the emission of greenhouse gases (i.e. CH4 and CO2) from natural water systems. At present, the occurrence characteristics, deposition fluxes, and driving force of OC components in the river-lake ecotone are unclear. Here, the tempo-spatial distribution of polycyclic aromatic hydrocarbons (PAHs) and Σn-alkanes (nC13–nC32) related to organic nitrogen (ON) and OC in the estuarine sediments from Poyang Lake and their sources and effects were investigated. The ON and OC contents in the sediment cores ranged from 0.35−0.64 % and 1.9–4.24 %, respectively, and the PAH fluxes varied between 672.19 and 24703.97 ng cm−2 a-1, showing an upward risk to the water bodies and human health. In particular, it is worth noting that the 5–6 ring monomers were dominant, accounting for 66.48 % of the ΣPAHs in the sediment profile. The sources were mainly derived from biomass combustion and automobile exhaust emissions, while the latter proportion has increased in recent decades. In the sediment profile, the n-alkane abundance peak was found at nC31 with a marked odd-even predominance. The deposition flux for Σn-alkanes ranged between 10.92 and 73.46 μg cm−2 a-1. In addition, long-chain monomers dominated the Σn-alkanes and were found to be herbaceous in origin. An analysis of the environmental indicators in the sediment layers showed that forest restoration and the eutrophication of water bodies caused by anthropogenic stress and climate change were mainly responsible for the relatively large contribution made by vascular plants and autochthonous organisms.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1470160X23001188Test; https://doaj.org/toc/1470-160XTest

الوصول الحر: https://doaj.org/article/369c1501694842259eaa922c6f10039dTest

المؤلفون: Kangkang Yu, Yanqin Zhang, Xinghua He, Zihan Zhao, Mingli Zhang, Yan Chen, Xiulu Lang, Yanhua Wang

المصدر: Ecological Indicators, Vol 138, Iss , Pp 108796- (2022)

مصطلحات موضوعية: Taihu Lake, Sediments, Light and heavy fraction of organic carbon, Influencing factors, Redundancy analysis, Ecology, QH540-549.5

الوصف: The rapid economic development in the Yangtze River Delta has affected the carbon (C) pool in lake sediments, which has become a serious environmental management issue. To explore the characteristics of composition, spatial distribution and burial of organic C (OC) in lacustrine sediments, a typical shallow lake was targeted to compare the heavy fraction OC (HFOC), light fraction OC (LFOC), and dissolved OC (DOC) in the surface sediments of different lake areas using the specific gravity separation approach. The effects of environmental factors on the form of OC present were identified by a redundancy analysis. The results showed a regional difference of DOC, LFOC, and HFOC concentrations in Taihu Lake, and HFOC was dominant in the OC buried in lake sediments. The deposition rate of OC in Taihu Lake sediments varied in a ranged of 31.91–114.47 g C m−2 a-1. The LFOC levels in the sediments were lower than those in other lakes. Both sedimentary LFOC and HFOC were significantly correlated with the nitrogen (N) content, indicating that the C sink was enhanced by an increase in N in the sediments. The grass-type lake zone stimulated C accumulation in Taihu Lake sediments.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1470160X22002679Test; https://doaj.org/toc/1470-160XTest

الوصول الحر: https://doaj.org/article/addf5d73012d4377a8c9ec127b049d7eTest

المؤلفون: Liangqun Li, Kangkang Yu, Zhengchang Mo, Keling Yang, Fuxue Chen, Juan Yang

المصدر: Molecules, Vol 28, Iss 4, p 1544 (2023)

مصطلحات موضوعية: Pyrola corbieri Levl, polysaccharide, purification, structural analysis, neurotrophic activity, Organic chemistry, QD241-441

الوصف: Pyrola corbieri Levl has been used to strengthen bones and nourish the kidney (the kidney governs the bone and is beneficial to the brain) by the local Miao people in China. However, the functional components and neurotrophic activity have not been reported. A new acidic homogeneous heteropolysaccharide named LTC-1 was obtained and characterized by periodate oxidation, Smith degradation, partial acid hydrolysis, GC–MS spectrometry, methylation analysis, and Fourier transform infrared spectroscopy, and its molecular weight was 3239 Da. The content of mannuronic acid (Man A) in LTC-1 was 46%, and the neutral sugar was composed of L-rhamnose (L-Rha), L-arabinose (L-Ara), D-xylose (D-Xyl), D-mannose (D-Man), D-glucose (D-Glc) and D-galactose (D-Gal) with a molar ratio of 1.00:3.63:0.86:1.30:6.97:1.30. The main chain of LTC-1 was composed of Glc, Gal, Man, Man A and the branched chain Ara, Glc, Gal. The terminal residues were composed of Glc and Gal. The main chain and branched chains were linked by (1→5)-linked-Ara, (1→3)-linked-Glc, (1→4)-linked-Glc, (1→6)-linked-Glc, (1→3)-linked-Gal, (1→6)-linked-Gal, (1→3, 6)-linked-Man and ManA. Meanwhile, neurotrophic activity was evaluated through PC12 and primary hippocampal neuronal cell models. LTC-1 exhibited neurotrophic activity in a concentration-dependent manner, which significantly induced the differentiation of PC12 cells, promoted the neurite outgrowth of PC12 cells, enhanced the formation of the web architecture of dendrites, and increased the density of dendritic spines in hippocampal neurons and the expression of PSD-95. These results displayed significant neurotrophic factor-like activity of LTC-1, which suggests that LTC-1 is a potential treatment option for neurodegenerative diseases.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1420-3049/28/4/1544Test; https://doaj.org/toc/1420-3049Test

الوصول الحر: https://doaj.org/article/79f5a8ef1c0e4af1bc035f12c379527dTest

المؤلفون: Yahui Zheng, Chong Huang, Lu Lu, Kangkang Yu, Jing Zhao, Mingquan Chen, Lu Liu, Qingfeng Sun, Zhifei Lin, Jianming Zheng, Jinhong Chen, Jubo Zhang

المصدر: Journal of Hematology & Oncology, Vol 14, Iss 1, Pp 1-18 (2021)

مصطلحات موضوعية: Stomatin-like protein 2 (STOML2), Hepatocellular carcinoma (HCC), PTEN-induced putative kinase 1 (PINK1), Mitophagy, Lenvatinib, Diseases of the blood and blood-forming organs, RC633-647.5, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background Dysregulation of both mitochondrial biogenesis and mitophagy is critical to sustain oncogenic signaling pathways. However, the mechanism of mitophagy in promoting hepatocellular carcinoma (HCC) progression remains poorly understood. In this study, we investigated the clinical significance and biological involvement of mitochondrial inner membrane protein STOML2 in HCC. Methods STOML2 was identified by gene expression profiles of HCC tissues and was measured in tissue microarray and cell lines. Gain/loss-of-function experiment was applied to study the biological function of STOML2 in HCC. Flow cytometry, Western blotting, laser confocal microscopy, transmission electron microscopy, and co-immunoprecipitation were used to detect and analyze mitophagy. ChIP and luciferase reporter assay were conducted to evaluate the relationship between STOML2 and HIF-1α. The sensitivity to lenvatinib was assessed in HCC both in vitro and in vivo. Results Increased expression of STOML2 was found in HCC compared with paired peritumoral tissues. It was more significant in HCC with metastasis and correlated with worse overall survival and higher probability of recurrence after hepatectomy. Upregulation of STOML2 accelerated HCC cells colony formation, migration and invasion. Mechanically, TCGA dataset-based analysis showed enrichment of autophagy-related pathways in STOML2 highly-expressed HCC. Next, STOML2 was demonstrated to interact and stabilize PINK1 under cellular stress, amplify PINK1-Parkin-mediated mitophagy and then promote HCC growth and metastasis. Most interestingly, HIF-1α was upregulated and transcriptionally increased STOML2 expression in HCC cells under the treatment of lenvatinib. Furthermore, higher sensitivity to lenvatinib was found in HCC cells when STOML2 was downregulated. Combination therapy with lenvatinib and mitophagy inhibitor hydroxychloroquine obtained best efficacy. Conclusions Our findings suggested that STOML2 could amplify mitophagy through interacting and stabilizing PINK1, which promote HCC metastasis and modulate the response of HCC to lenvatinib. Combinations of pharmacologic inhibitors that concurrently block both angiogenesis and mitophagy may serve as an effective treatment for HCC.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1756-8722Test

الوصول الحر: https://doaj.org/article/422cf11815d34b31aac6d2661d57bc85Test

المؤلفون: Jinliang Chen, Ning Chris Chen, Kangkang Yu, Colin Michael Hall

المصدر: Frontiers in Psychology, Vol 12 (2021)

مصطلحات موضوعية: social identity, corporate social responsibility, business performance, entrepreneurs’ well-being, stakeholder theory, Psychology, BF1-990

الوصف: Although the impact of entrepreneurs’ social identity on successful entrepreneurship has attracted much scholarly attention, it is often to evaluate successful entrepreneurship through direct channel to financial performance. Recently, there is a growing body of researches beginning to pay attention to the impact of entrepreneurs’ social identity on corporate social responsibility (CSR) regarded as indirect social aspect channel to successful entrepreneurship. However, little is known regarding how entrepreneurs’ Darwinian social identity affects CSR, which in turn, affects business performance. This study addresses this issue by combining stakeholder theory with social identity theory, to investigate the relationship between entrepreneurs’ Darwinian social identity and business performance via CSR. In addition, the moderating effect of entrepreneur’s well-being is further examined to uncover the interaction effect of the individual psychological resource on business performance. The empirical results indicate that entrepreneurs’ Darwinian social identity contributes positively to CSR, so as further to business performance. In addition, this relationship is further found to be significantly moderated by entrepreneurs’ well-being. The results indicate that entrepreneurs can achieve business success via CSR, by which entrepreneurs can further acquire successful entrepreneurship through caring more about their well-being.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fpsyg.2021.781399/fullTest; https://doaj.org/toc/1664-1078Test

الوصول الحر: https://doaj.org/article/0efc25b910ac4f7896261351ddff4ef6Test

المؤلفون: Yanhua Wang, Yu Huang, Jiaming Tian, Chunhua Li, Kangkang Yu, Mingli Zhang, Xiulu Lang, Tian Sun

المصدر: Ecological Indicators, Vol 130, Iss , Pp 108090- (2021)

مصطلحات موضوعية: Sediment, Biomarker, Organic carbon, Anthropogenic activities, Dongting Lake, Ecology, QH540-549.5

الوصف: The extensive anthropogenic activities and their potential impacts on lakes during the Anthropocene could be recorded in the sediments. In this study, the composition of biomarkers (i.e., n-alkanes and n-fatty acids), total organic carbon (TOC), and particle size in 210Pb-dated sediment cores from West Dongting Lake were investigated to trace the historical trend of anthropogenic inputs and their environmental significance. The TOC content was in the range of 3.15–27.32 mg g−1. The n-alkanes and n-fatty acids were distributed from C11–C33 and C12:0–C34:0, respectively. The short- and long-chain biomarkers accounted for a significant proportion of the total. Aquatic plants and algae living in West Dongting Lake were the main source (short-chain) followed by leaf waxes derived from terrestrial C3 plants (long-chain), as demonstrated by their concentrations. The variations of the key indicators i.e., the carbon preference index (CPI), aquatic plant n-alkane proxy (Paq), vascular plant n-alkane proxy (Pwax), and the ratio of unit short- to long-chain n-alkanes (L/H) also indicated that the aliphatic hydrocarbon fractions were mainly composed of short- and long-chain biomarkers. The sedimentary biomarkers had a significant temporal heterogeneity, which was controlled by different factors and sedimentary processes. Fine particles (

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1470160X2100755XTest; https://doaj.org/toc/1470-160XTest

الوصول الحر: https://doaj.org/article/c03da45472734d40b374be4a5758e05bTest

المؤلفون: Yuwei Gu, Chao Wang, Shengsen Chen, Jia Tang, Xiaoxiao Guo, Wei Hu, An Cui, Dian Zhang, Kangkang Yu, Mingquan Chen

المصدر: Frontiers in Genetics, Vol 12 (2021)

مصطلحات موضوعية: hepatocellular carcinoma, pseudogene, PPIA, chemokine, macrophage, Genetics, QH426-470

الوصف: The burden of hepatocellular carcinoma (HCC) worldwide is increasing over time, while the underlying molecular mechanism of HCC development is still under exploration. Pseudogenes are classified as a special type of long non-coding RNAs (lncRNAs), and they played a vital role in regulating tumor-associated gene expression. Here, we report that a pseudogene peptidylprolyl isomerase A pseudogene 22 (PPIAP22) and its parental gene peptidylprolyl isomerase A (PPIA) were upregulated in HCC and were associated with the clinical outcomes of HCC. Further investigation revealed that PPIAP22 might upregulate the expression of PPIA through sponging microRNA (miR)-197-3p, behaving as competing endogenous RNA (ceRNA). PPIA could participate in the development of HCC by regulating mRNA metabolic process and tumor immunity based on the functional enrichment analysis. We also found a strong correlation between the expression levels of PPIA and the immune cell infiltration or the expression of chemokines, especially macrophage, C-C motif chemokine ligand 15 (CCL15), and C-X-C motif chemokine ligand 12 (CXCL12). Our findings demonstrate that the PPIAP22/miR-197-3p/PPIA axis plays a vital role in the progression of HCC by increasing the malignancy of tumor cells and regulating the immune cell infiltration, especially macrophage, through CCL15-CCR1 or CXCL12-CXCR4/CXCR7 pathways.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fgene.2021.604461/fullTest; https://doaj.org/toc/1664-8021Test

الوصول الحر: https://doaj.org/article/6b437af5955d41e3ad7e0715fd7c2badTest