المؤلفون: Oskolkov, Nikolay, Santel, Malgorzata, Parikh, Hemang M., Ekström, Ola, Camp, Gray J., Miyamoto-Mikami, Eri, Ström, Kristoffer, Mir, Bilal Ahmad, Kryvokhyzha, Dmytro, Lehtovirta, Mikko, Kobayashi, Hiroyuki, Kakigi, Ryo, Naito, Hisashi, Eriksson, Karl Fredrik, Nystedt, Björn, Fuku, Noriyuki, Treutlein, Barbara, Pääbo, Svante, Hansson, Ola

المصدر: Skeletal Muscle EXODIAB: Excellence of Diabetes Research in Sweden. 12:1-9

مصطلحات موضوعية: Naturvetenskap, Biologi, Bioinformatik och systembiologi, Natural Sciences, Biological Sciences, Bioinformatics and Systems Biology, Medicin och hälsovetenskap, Klinisk medicin, Klinisk laboratoriemedicin, Medical and Health Sciences, Clinical Medicine, Clinical Laboratory Medicine

الوصف: Background: Skeletal muscle fiber type distribution has implications for human health, muscle function, and performance. This knowledge has been gathered using labor-intensive and costly methodology that limited these studies. Here, we present a method based on muscle tissue RNA sequencing data (totRNAseq) to estimate the distribution of skeletal muscle fiber types from frozen human samples, allowing for a larger number of individuals to be tested. Methods: By using single-nuclei RNA sequencing (snRNAseq) data as a reference, cluster expression signatures were produced by averaging gene expression of cluster gene markers and then applying these to totRNAseq data and inferring muscle fiber nuclei type via linear matrix decomposition. This estimate was then compared with fiber type distribution measured by ATPase staining or myosin heavy chain protein isoform distribution of 62 muscle samples in two independent cohorts (n = 39 and 22). Results: The correlation between the sequencing-based method and the other two were rATPas = 0.44 [0.13–0.67], [95% CI], and rmyosin = 0.83 [0.61–0.93], with p = 5.70 × 10–3 and 2.00 × 10–6, respectively. The deconvolution inference of fiber type composition was accurate even for very low totRNAseq sequencing depths, i.e., down to an average of ~ 10,000 paired-end reads. Conclusions: This new method (https://github.com/OlaHanssonLab/PredictFiberTypeTest) consequently allows for measurement of fiber type distribution of a larger number of samples using totRNAseq in a cost and labor-efficient way. It is now feasible to study the association between fiber type distribution and e.g. health outcomes in large well-powered studies.

الوصول الحر: https://lup.lub.lu.se/record/e7d7060e-5cf8-4bd3-b4ea-7b2a52d957e3Test
http://dx.doi.org/10.1186/s13395-022-00299-4Test

المؤلفون: Sugihara, Masami, Kakigi, Ryo, Murayama, Takashi, Miida, Takashi, Sakurai, Takashi, Morimoto, Sachio, Kurebayashi, Nagomi

المصدر: Biophysical Journal ; volume 122, issue 3, page 123a ; ISSN 0006-3495

مصطلحات موضوعية: Biophysics

الإتاحة: https://doi.org/10.1016/j.bpj.2022.11.833Test
https://api.elsevier.com/content/article/PII:S0006349522017490?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0006349522017490?httpAccept=text/plainTest

المؤلفون: Chang, Shuo-wen, Yoshihara, Toshinori, Tsuzuki, Takamasa, Natsume, Toshiharu, Kakigi, Ryo, Machida, Shuichi, Naito, Hisashi

المساهمون: Asakura, Atsushi, Japan Society for the Promotion of Science KAKENHI, Ministry of Education, Culture, Sports, Science and Technology Supported Program for the Strategic Research Foundation at Private Universities

المصدر: PLOS ONE ; volume 17, issue 2, page e0264171 ; ISSN 1932-6203

الوصف: We investigated whether time-of-day dependent changes in the rat soleus (SOL) muscle size, after eccentric exercises, operate via the mechanistic target of rapamycin (mTOR) signaling pathway. For our first experiment, we assigned 9-week-old male Wistar rats randomly into four groups: light phase (zeitgeber time; ZT6) non-trained control, dark phase (ZT18) non-trained control, light phase-trained, and dark phase-trained. Trained animals performed 90 min of downhill running once every 3 d for 8 weeks. The second experiment involved dividing 9-week-old male Wistar rats to control and exercise groups. The latter were subjected to 15 min of downhill running at ZT6 and ZT18. The absolute (+12.8%) and relative (+9.4%) SOL muscle weights were higher in the light phase-trained group. p70S6K phosphorylation ratio was 42.6% higher in the SOL muscle of rats that had exercised only in light (non-trained ZT6). Collectively, the degree of muscle hypertrophy in SOL is time-of-day dependent, perhaps via the mTOR/p70S6K signaling.

الإتاحة: https://doi.org/10.1371/journal.pone.0264171Test

المؤلفون: MIYAMOTO-MIKAMI, ERI, KUMAGAI, HIROSHI, TANISAWA, KUMPEI, TAGA, YUKI, HIRATA, KOSUKE, KIKUCHI, NAOKI, KAMIYA, NOBUHIRO, KAWAKAMI, RYOKO, MIDORIKAWA, TAISHI, KAWAMURA, TAKUJI, KAKIGI, RYO, NATSUME, TOSHIHARU, ZEMPO, HIROFUMI, SUZUKI, KOYA, KOHMURA, YOSHIMITSU, MIZUNO, KAZUNORI, TORII, SUGURU, SAKAMOTO, SHIZUO, OKA, KOICHIRO, HIGUCHI, MITSURU, NAITO, HISASHI, MIYAMOTO, NAOKAZU, FUKU, NORIYUKI

المصدر: Medicine & Science in Sports & Exercise ; volume 53, issue 9, page 1855-1864 ; ISSN 1530-0315 0195-9131

الوصف: Purpose We aimed to investigate the hypothesis that type I collagen plays a role in increasing bone mineral density (BMD) and muscle stiffness, leading to low and high risks of fatigue fracture and muscle injury, respectively, in athletes. As a potential mechanism, we focused on the effect of the type I collagen alpha 1 chain gene ( COL1A1 ) variant associated with transcriptional activity on bone and skeletal muscle properties. Methods The association between COL1A1 rs1107946 and fatigue fracture/muscle injury was evaluated in Japanese athletes. Effects of the polymorphism on tissue properties (BMD and muscle stiffness) and type I collagen α1/α2 chain ratios in muscles were examined in Japanese nonathletes. Results The C-allele carrier frequency was greater in female athletes with fatigue fracture than in those without (odds ratio = 2.44, 95% confidence interval [CI] = 1.17–5.77) and lower in female athletes with muscle injury than in those without (odds ratio = 0.46, 95% CI = 0.24–0.91). Prospective validation analysis confirmed that in female athletes, muscle injury was less frequent in C-allele carriers than in AA genotype carriers (multivariable-adjusted hazard ratio = 0.27, 95% CI = 0.08–0.96). Among female nonathletes, the C-allele of rs1107946 was associated with lower BMD and lower muscle stiffness. Muscle biopsy revealed that C-allele carriers tended to have a larger type I collagen α1/α2 chain ratio than AA genotype carriers (2.24 vs 2.05, P = 0.056), suggesting a higher proportion of type I collagen α1 homotrimers. Conclusion The COL1A1 rs1107946 polymorphism exerts antagonistic effects on fatigue fracture and muscle injury among female athletes by altering the properties of these tissues, potentially owing to increased levels of type I collagen α1 chain homotrimers.

الإتاحة: https://doi.org/10.1249/mss.0000000000002658Test

المؤلفون: Yvert, Thomas Paul, Miyamoto-Mikami, Eri, Tobina, Takuro, Shiose, Keisuke, Kakigi, Ryo, Tsuzuki, Takamasa, Takaragawa, Mizuki, Ichinoseki-Sekine, Noriko, Pérez Ruiz, Margarita, Fuku, Noriyuki

مصطلحات موضوعية: Genética humana, Mujeres, Fisiología humana, Mujer

الوصف: PPARGC1A rs8192678 G/A (Gly482Ser) and NRF1 rs6949152 A/G polymorphisms have been associated with endurance athlete status, endurance performance phenotypes, and certain health-related markers of different pathologies such as metabolic syndrome, diabetes, and dyslipidemia. We hypothesized that they could be considered interesting candidates for explaining inter-individual variations in muscle fiber composition in humans. We aimed to examine possible associations of these polymorphisms with myosin heavy-chain (MHC) isoforms as markers of muscle fiber compositions in vastus lateralis muscle in a population of 214 healthy Japanese subjects, aged between 19 and 79 years. No significant associations were found in men for any measured variables. In contrast, in women, the PPARGC1A rs8192678 A/A genotype was significantly associated with a higher proportion of MHC-I (p = 0.042) and with a lower proportion of MHC-IIx (p = 0.033), and the NRF1 rs6949152 AA genotype was significantly associated with a higher proportion of MHC-I (p = 0.008) and with a lower proportion of MHC IIx (p = 0.035). In women, the genotype scores of the modes presenting the most significant results for PPARGC1A rs8192678 G/A (Gly482Ser) and NRF1 rs6949152 A/G polymorphisms were significantly associated with MHC-I (p = 0.0007) and MHC IIx (p = 0.0016). That is, women with combined PPARGC1A A/A and NRF1 A/A genotypes presented the highest proportion of MHC-I and the lowest proportion of MHC-IIx, in contrast to women with combined PPARGC1A GG+GA and NRF1 AG+GG genotypes, who presented the lowest proportion of MHC-I and the highest proportion of MHC-IIx. Our results suggest possible associations between these polymorphisms (both individually and in combination) and the inter-individual variability observed in muscle fiber composition in women, but not in men. ; JSPS KAKENHI (16KK0188 to N.F) ; MEXT-Supported Program for the Strategic Research Foundation at Private Universities (to Juntendo University and Fukuoka University) ; 4.096 JCR (2020) Q2, ...

العلاقة: Yvert, T., Miyamoto-Mikami, E., Tobina, T., Shiose, K., Kakigi, R., Tsuzuki, T., Takaragawa, M., Ichinoseki-Sekine, N., Pérez Ruiz, M., Kobayashi, H., Tanaka, H., Naito, H., & Fuku, N. (2020). PPARGC1A rs8192678 and NRF1 rs6949152 Polymorphisms Are Associated with Muscle Fiber Composition in Women. Genes, 11(9), 1012. https://doi.org/10.3390/genes11091012Test; http://hdl.handle.net/11268/9264Test

الإتاحة: https://doi.org/10.3390/genes11091012Test
http://hdl.handle.net/11268/9264Test

المؤلفون: Ogura, Yuji, Kakehashi, Chiaki, Yoshihara, Toshinori, Kurosaka, Mitsutoshi, Kakigi, Ryo, Higashida, Kazuhiko, Fujiwara, Sei-Etsu, Akema, Tatsuo, Funabashi, Toshiya

المساهمون: Asakura, Atsushi, Ministry of Education, Culture, Sports, Science and Technology, Japan Society for the Promotion of Science

المصدر: PLOS ONE ; volume 15, issue 10, page e0241382 ; ISSN 1932-6203

الإتاحة: https://doi.org/10.1371/journal.pone.0241382Test

المؤلفون: Ogura, Yuji, Yoshihara, Toshinori, Kurosaka, Mitsutoshi, Kakigi, Ryo, Fujiwara, Sei-etsu, Kakehashi, Chiaki, Higashida, Kazuhiko, Funabashi, Toshiya

المصدر: The FASEB Journal ; volume 34, issue S1, page 1-1 ; ISSN 0892-6638 1530-6860

الوصف: Ketogenic diet is an extremely high‐fat diet with low or absence of carbohydrate in the total nutrient. Thus, this diet causes ketone body production from the liver and shifts energy sources from carbohydrate to fat. Whereas previous studies have suggested that ketogenic diet ameliorates brain disorder such as drug‐resistant epilepsy, its influence on peripheral tissue is unclear. Skeletal muscle is an important organ for locomotion and metabolism. Recent studies have suggested that the ketogenic diet changes the energy metabolic properties in the skeletal muscle as well; however, the results are contradictory. Moreover, it remains unknown whether the ketogenic diet affects the muscle contractile function. Therefore, this study aimed to clarify the effect of ketogenic diet on muscle performance and biochemical adaptation in rat skeletal muscle. This study was approved by Institute for Animal Experimentation at St. Marianna University School of Medicine. Male Wistar rats (8 weeks of age) were assigned to either control group (CON) or ketogenic diet group (KD). CON and KD were fed for 4 weeks either control diet (10% fat, 10% protein, 80% carbohydrate) or ketogenic diet (90% fat, 10% protein, 0% carbohydrate), respectively. Body mass was recorded once a week. After the 4‐week feeding period, the animals were anesthetized, and the blood sample was collected to analyze blood ketone concentration. The right extensor digitrum longus (EDL) muscle was used to evaluate the twitch force, tetanic force, and fatigue ex vivo. The left EDL was homogenized and used to analyze the myosin heavy chain composition, the protein expression of metabolic enzymes, and the citrate synthase activity. Body mass was lower in KD than CON at third and fourth week of the feeding period. Blood ketone concentration in KD was significantly higher than CON. There was no statistical difference in the twitch and tetanic forces as well as muscle fatigue between CON and KD. However, the citrate synthase activity in KD was significantly higher than ...

الإتاحة: https://doi.org/10.1096/fasebj.2020.34.s1.09047Test

المؤلفون: Tsuzuki, Takamasa, Kobayashi, Hiroyuki, Yoshihara, Toshinori, Kakigi, Ryo, Ichinoseki-Sekine, Noriko, Naito, Hisashi

المصدر: Cell Stress & Chaperones, 2017 Mar 01. 22(2), 263-269.

الوصول الحر: https://www.jstor.org/stable/44848854Test

المؤلفون: Sugihara, Masami, Kakigi, Ryo, Murayama, Takashi, Miida, Takashi, Sakurai, Takashi, Morimoto, Sachio, Kurebayashi, Nagomi

المصدر: Biophysical Journal ; volume 116, issue 3, page 231a ; ISSN 0006-3495

مصطلحات موضوعية: Biophysics

الإتاحة: https://doi.org/10.1016/j.bpj.2018.11.1269Test
https://api.elsevier.com/content/article/PII:S0006349518325347?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0006349518325347?httpAccept=text/plainTest

المؤلفون: Guilherme, João Paulo Limongi França, Semenova, Ekaterina A., Zempo, Hirofumi, Martins, Gabriel L., Lancha Junior, Antonio H., Miyamoto-Mikami, Eri, Kumagai, Hiroshi, Tobina, Takuro, Shiose, Keisuke, Kakigi, Ryo, Tsuzuki, Takamasa, Ichinoseki-Sekine, Noriko, Kobayashi, Hiroyuki, Naito, Hisashi, Borisov, Oleg V., Kostryukova, Elena S., Kulemin, Nikolay A., Larin, Andrey K., Generozov, Edward V., Fuku, Noriyuki

المصدر: International Journal of Sports Physiology & Performance; Apr2021, Vol. 16 Issue 4, p489-495, 7p, 3 Charts, 1 Graph

مصطلحات موضوعية: SINGLE nucleotide polymorphisms, ATHLETES, COMPARATIVE studies, GENOMES, REPLICATION (Experimental design), ENDURANCE sports, ODDS ratio, ATHLETIC ability, SPRINTING

مصطلحات جغرافية: RUSSIA, BRAZIL

مستخلص: Purpose: To replicate previous genome-wide association study identified sprint-related polymorphisms in 3 different cohorts of top-level sprinters and to further validate the obtained results in functional studies. Methods: A total of 240 Japanese, 290 Russians, and 593 Brazilians were evaluated in a case-control approach. Of these, 267 were top-level sprint/power athletes. In addition, the relationship between selected polymorphisms and muscle fiber composition was evaluated in 203 Japanese and 287 Finnish individuals. Results: The G allele of the rs3213537 polymorphism was overrepresented in Japanese (odds ratio [OR]: 2.07, P =.024) and Russian (OR: 1.93, P =.027) sprinters compared with endurance athletes and was associated with an increased proportion of fast-twitch muscle fibers in Japanese (P =.02) and Finnish (P =.041) individuals. A meta-analysis of the data from 4 athlete cohorts confirmed that the presence of the G/G genotype rather than the G/A+A/A genotypes increased the OR of being a sprinter compared with controls (OR: 1.49, P =.01), endurance athletes (OR: 1.79, P =.001), or controls + endurance athletes (OR: 1.58, P =.002). Furthermore, male sprinters with the G/G genotype were found to have significantly faster personal times in the 100-m dash than those with G/A+A/A genotypes (10.50 [0.26] vs 10.76 [0.31], P =.014). Conclusion: The rs3213537 polymorphism found in the CPNE5 gene was identified as a highly replicable variant associated with sprinting ability and the increased proportion of fast-twitch muscle fibers, in which the homozygous genotype for the major allele (ie, the G/G genotype) is preferable for performance. [ABSTRACT FROM AUTHOR]

: Copyright of International Journal of Sports Physiology & Performance is the property of Human Kinetics Publishers, Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)