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1دورية أكاديمية
المؤلفون: Braś, Grażyna, Satała, Dorota, Juszczak, Magdalena, Kulig, Kamila, Wronowska, Ewelina, Bednarek, Aneta, Zawrotniak, Marcin, Rąpała-Kozik, Maria, Karkowska-Kuleta, Justyna
مصطلحات موضوعية: Candida, candidiasis, proteinases, Saps, proteolysis, protein degradation, virulence
الوصف: Extracellular proteases are key factors contributing to the virulence of pathogenic fungi from the genus Candida. Their proteolytic activities are crucial for extracting nutrients from the external environment, degrading host defenses, and destabilizing the internal balance of the human organism. Currently, the enzymes most frequently described in this context are secreted aspartic proteases (Saps). This review comprehensively explores the multifaceted roles of Saps, highlighting their importance in biofilm formation, tissue invasion through the degradation of extracellular matrix proteins and components of the coagulation cascade, modulation of host immune responses via impairment of neutrophil and monocyte/macrophage functions, and their contribution to antifungal resistance. Additionally, the diagnostic challenges associated with Candida infections and the potential of Saps as biomarkers were discussed. Furthermore, we examined the prospects of developing vaccines based on Saps and the use of protease inhibitors as adjunctive therapies for candidiasis. Given the complex biology of Saps and their central role in Candida pathogenicity, a multidisciplinary approach may pave the way for innovative diagnostic strategies and open new opportunities for innovative clinical interventions against candidiasis.
العلاقة: International Journal of Molecular Sciences, T. 25, nr 9; https://ruj.uj.edu.pl/handle/item/339249Test
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3دورية أكاديمية
المؤلفون: Juszczak, Magdalena, Zawrotniak, Marcin, Rapala-Kozik, Maria
المساهمون: Narodowe Centrum Nauki
المصدر: Frontiers in Immunology ; volume 15 ; ISSN 1664-3224
مصطلحات موضوعية: Immunology, Immunology and Allergy
الوصف: Candida albicans remains the predominant cause of fungal infections, where adhered microbial cells form biofilms - densely packed communities. The central feature of C. albicans biofilms is the production of an extracellular matrix (ECM) consisting of polymers and extracellular nucleic acids (eDNA, eRNA), which significantly impedes the infiltration of host cells. Neutrophils, as crucial players in the innate host defense, employ several mechanisms to eradicate the fungal infection, including NETosis, endocytosis, or the release of granules containing, among others, antimicrobial peptides (AMPs). The main representative of these is the positively charged peptide LL-37 formed from an inactive precursor (hCAP18). In addition to its antimicrobial functions, this peptide possesses a propensity to interact with negatively charged molecules, including nucleic acids. Our in vitro studies have demonstrated that LL-37 contacting with C. albicans nucleic acids, isolated from biofilm, are complexed by the peptide and its shorter derivatives, as confirmed by electrophoretic mobility shift assays. We indicated that the generation of the complexes induces discernible alterations in the neutrophil response to fungal nucleic acids compared to the effects of unconjugated molecules. Our analyses involving fluorescence microscopy, flow cytometry, and Western blotting revealed that stimulation of neutrophils with DNA:LL-37 or RNA:LL-37 complexes hamper the activation of pro-apoptotic caspases 3 and 7 and fosters increased activation of anti-apoptotic pathways mediated by the Mcl-1 protein. Furthermore, the formation of complexes elicits a dual effect on neutrophil immune response. Firstly, they facilitate increased nucleic acid uptake, as evidenced by microscopic observations, and enhance the pro-inflammatory response, promoting IL-8 production. Secondly, the complexes detection suppresses the production of reactive oxygen species and attenuates NETosis activation. In conclusion, these findings may imply that the neutrophil immune ...
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4دورية أكاديمية
المؤلفون: Guevara-Lora, Ibeth, Braś, Grażyna, Juszczak, Magdalena, Karkowska-Kuleta, Justyna, Górecki, Andrzej, Manrique-Moreno, Marcela, Dymek, Jakub, Pyza, Elżbieta, Kozik, Andrzej, Rąpała-Kozik, Maria
مصطلحات موضوعية: antimicrobial peptides, cecropin, Candida, candidiasis, biofilm
الوصف: The recent progressive increase in the incidence of invasive fungal infections, especially in immunocompromised patients, makes the search for new therapies crucial in the face of the growing drug resistance of prevalent nosocomial yeast strains. The latest research focuses on the active compounds of natural origin, inhibiting fungal growth, and preventing the formation of fungal biofilms. Antimicrobial peptides are currently the subject of numerous studies concerning effective antifungal therapy. In the present study, the antifungal properties of two synthetic peptides $\Delta$M3, $\Delta$M4) derived from an insect antimicrobial peptide - cecropin D - were investigated. The fungicidal activity of both compounds was demonstrated against the yeast forms of Candida albicans, Candida tropicalis, and Candida parapsilosis, reaching a MFC99.9 in the micromolar range, while Candida glabrata showed greater resistance to these peptides. The scanning electron microscopy revealed a destabilization of the yeast cell walls upon treatment with both peptides; however, their effectiveness was strongly modified by the presence of salt or plasma in the yeast environment. The transition of C. albicans cells from yeast to filamentous form, as well as the formation of biofilms, was effectively reduced by ΔM4. Mature biofilm viability was inhibited by a higher concentration of this peptide and was accompanied by increased ROS production, activation of the GPX3 and SOD5 genes, and finally, increased membrane permeability. Furthermore, both peptides showed a synergistic effect with caspofungin in inhibiting the metabolic activity of C. albicans cells, and an additive effect was also observed for the mixtures of peptides with amphotericin B. The results indicate the possible potential of the tested peptides in the prevention and treatment of candidiasis.
العلاقة: Frontiers in Microbiology, T. 13; https://ruj.uj.edu.pl/xmlui/handle/item/306102Test
الإتاحة: https://doi.org/10.3389/fmicb.2022.1045984Test
https://ruj.uj.edu.pl/xmlui/handle/item/306102Test -
5دورية أكاديمية
مصطلحات موضوعية: neutrophils, rhinitis, nasal secretion, NETs, elastase, myeloperoxidase
الوصف: Neutrophil extracellular traps (NETs) are structures released by neutrophils in response to various infections. NETs have a biocidal role and have been demonstrated to be effective against bacteria, fungi, viruses, and parasites. Depending on the situation, NETs can protect the host from pathogen invasion or contribute to the development of autoimmune diseases such as cystic fibrosis and rheumatoid arthritis. In this study, we aimed to investigate the occurrence of NET as one of the components in upper respiratory tract secretions in infectious and allergic diseases. Nasal mucus was collected from donors diagnosed with infectious rhinitis or allergic rhinitis. The extracellular DNA content was determined using SytoxGreen staining, and the total protein pool was determined using the microBCA method. Micrococcal nuclease was used to digest the samples and ELISA was employed to identify the NET proteins. The enzymatic activity of elastase was determined. Our findings showed that nasal mucus collected from patients with infectious rhinosinusitis contained extracellular DNA that could come from a variety of sources, responsible for increasing the density and viscosity of secretions, as well as NETs proteins. The identified enzymatic activity of NET elastase indicates the possible irritation of nasal tissues. However, the DNA content was not identified in the samples from allergic patients. In addition, we have shown in preliminary studies that therapy using N-acetylcysteine can liquefy nasal secretions.The study suggests that the composition of nasal mucus varies according to the cause of mucosal irritation. The presence of DNA and NET proteins can have severe consequences for the therapeutic process prolonging treatment. The low viscosity of nasal mucus in allergic patients facilitates mucosal flushing and the removal of allergens. Understanding the occurrence and role of NETs in various respiratory diseases is critical for developing effective treatment strategies that consider the complex interaction between the ...
العلاقة: Frontiers in Immunology, T. 14; https://ruj.uj.edu.pl/xmlui/handle/item/323368Test; https://www.frontiersin.org/articles/10.3389/fimmu.2023.1295921/abstractTest
الإتاحة: https://doi.org/10.3389/fimmu.2023.1295921Test
https://ruj.uj.edu.pl/xmlui/handle/item/323368Test -
6دورية أكاديمية
المؤلفون: Bras, Grazyna, Satala, Dorota, Juszczak, Magdalena, Kulig, Kamila, Wronowska, Ewelina, Bednarek, Aneta, Zawrotniak, Marcin, Rapala-Kozik, Maria, Karkowska-Kuleta, Justyna
المصدر: International Journal of Molecular Sciences; May2024, Vol. 25 Issue 9, p4775, 25p
مصطلحات موضوعية: ASPARTIC proteinases, CANDIDIASIS, EXTRACELLULAR matrix proteins, ECHINOCANDINS, PROTEINASES, PATHOGENIC fungi, FUNGAL virulence
مستخلص: Extracellular proteases are key factors contributing to the virulence of pathogenic fungi from the genus Candida. Their proteolytic activities are crucial for extracting nutrients from the external environment, degrading host defenses, and destabilizing the internal balance of the human organism. Currently, the enzymes most frequently described in this context are secreted aspartic proteases (Saps). This review comprehensively explores the multifaceted roles of Saps, highlighting their importance in biofilm formation, tissue invasion through the degradation of extracellular matrix proteins and components of the coagulation cascade, modulation of host immune responses via impairment of neutrophil and monocyte/macrophage functions, and their contribution to antifungal resistance. Additionally, the diagnostic challenges associated with Candida infections and the potential of Saps as biomarkers were discussed. Furthermore, we examined the prospects of developing vaccines based on Saps and the use of protease inhibitors as adjunctive therapies for candidiasis. Given the complex biology of Saps and their central role in Candida pathogenicity, a multidisciplinary approach may pave the way for innovative diagnostic strategies and open new opportunities for innovative clinical interventions against candidiasis. [ABSTRACT FROM AUTHOR]
: Copyright of International Journal of Molecular Sciences is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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7دورية أكاديمية
المؤلفون: Karkowska-Kuleta, Justyna, Juszczak, Magdalena, Seweryn-Ożóg, Karolina, Satała, Dorota, Zawrotniak, Marcin, Wronowska, Ewelina, Bocheńska, Oliwia, Kozik, Andrzej, Nobbs, Angela H., Gogól, Mariusz, Rąpała-Kozik, Maria
مصطلحات موضوعية: neutrophil extracellular traps (NETs), Candida albicans, fungal cell wall proteins, fungal adhesins, moonlighting proteins, granular proteins, elastase, myeloperoxidase, cathelicidin LL-37, histones
العلاقة: Cells, T. 10, nr 10; https://ruj.uj.edu.pl/xmlui/handle/item/284390Test
الإتاحة: https://doi.org/10.3390/cells10102736Test
https://ruj.uj.edu.pl/xmlui/handle/item/284390Test -
8دورية أكاديمية
مصطلحات موضوعية: neutrophil extracellular traps (NETs), Candida albicans, biofilms, extracellular nucleic acids, extracellular matrix (ECM)
العلاقة: Frontiers in Cellular and Infection Microbiology, T. 11; https://ruj.uj.edu.pl/xmlui/handle/item/276533Test
الإتاحة: https://doi.org/10.3389/fcimb.2021.681030Test
https://ruj.uj.edu.pl/xmlui/handle/item/276533Test -
9كتاب
المؤلفون: Rąpała-Kozik, Maria, Zawrotniak, Marcin, Gogól, Mariusz, Bartnicka, Dominika, Satała, Dorota, Juszczak, Magdalena, Karkowska-Kuleta, Justyna, Kozik, Andrzej
مصطلحات موضوعية: Candida albicans, biofilm, aerobic and anaerobic bacteria, quorum-sensing, host responses, anti-biofilm therapies
العلاقة: Candida albicans, Sandai Doblin, s. 119-142; https://ruj.uj.edu.pl/xmlui/handle/item/129716Test
الإتاحة: https://doi.org/10.5772/intechopen.81537Test
https://ruj.uj.edu.pl/xmlui/handle/item/129716Test -
10دورية أكاديمية
العنوان البديل: Film as an Illustration of Social Roles. The Image of the Mother in Selected Films by Alfred Hitchcock. (English)
المؤلفون: JUSZCZAK, MAGDALENA
المصدر: Studia Edukacyjne; 2023, Issue 71, p259-284, 26p
مستخلص: Popular culture texts, including films, can be an area of research reflection, e.g. in terms of social phenomena, interpersonal relations, and the interpretation of social roles. The article analyzes the content of selected films by Alfred Hitchcock. The aim of the study was to verify the presentation of the mother-child relationship and the role of the mother. For this purpose, research questions were posed: What image of the mother is presented in Alfred Hitchcock's films, what are the characteristics of the mother in Alfred Hitchcock's films, what are the similarities between the image of mother presented by Alfred Hitchcock and socially expected features, what are the differences between the mother's image presented by Alfred Hitchcock maternal image and socially expected traits? Theses have been developed to answer the above questions and have been verified. A thorough analysis led to conclusions which indicate that the role of the mother had pathological features; kind of pattern of negative traits that the film women took on crystallized. Based on the research, the author defined the role as a "Hitchcock mother type". [ABSTRACT FROM AUTHOR]
: Copyright of Studia Edukacyjne is the property of Adam Mickiewicz University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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