المؤلفون: Junzhang Chen, Shiran Sun, Hui Li, Xiong Cai, Chidan Wan

المصدر: Frontiers in Immunology, Vol 15 (2024)

مصطلحات موضوعية: hepatocellular carcinoma, sorafenib resistance, IL-22, CD155, NK cell, Immunologic diseases. Allergy, RC581-607

الوصف: IntroductionSorafenib is currently the first-line treatment for patients with advanced hepatocellular carcinoma (HCC). Nevertheless, sorafenib resistance remains a huge challenge in the clinic. Therefore, it is urgent to elucidate the mechanisms underlying sorafenib resistance for developing novel treatment strategies for advanced HCC. In this study, we aimed to investigate the role and mechanisms of interleukin-22 (IL-22) in sorafenib resistance in HCC.MethodsThe in vitro experiments using HCC cell lines and in vivo studies with a nude mouse model were used. Calcium staining, chromatin immunoprecipitation, lactate dehydrogenase release and luciferase reporter assays were employed to explore the expression and roles of IL-22, STAT3 and CD155 in sorafenib resistance.ResultsOur clinical results demonstrated a significant correlation between elevated IL-22 expression and poor prognosis in HCC. Analysis of transcriptomic data from the phase-3 STORM-trial (BIOSTORM) suggested that STAT3 signaling activation and natural killer (NK) cell infiltration may associate sorafenib responses. STAT3 signaling could be activated by IL-22 administration in HCC cells, and then enhanced sorafenib resistance in HCC cells by promoting cell proliferation and reducing apoptosis in vitro and in vivo. Further, we found IL-22/STAT3 axis can transcriptionally upregulate CD155 expression in HCC cells, which could significantly reduce NK cell-mediated HCC cell lysis in a co-culture system.ConclusionsCollectively, IL-22 could contribute to sorafenib resistance in HCC by activating STAT3/CD155 signaling axis to decrease the sensitivities of tumor cells to sorafenib-mediated direct cytotoxicity and NK cell-mediated lysis. These findings deepen the understanding of how sorafenib resistance develops in HCC in terms of IL-22/STAT3 signaling pathway, and provide potential targets to overcome sorafenib resistance in patients with advanced HCC.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fimmu.2024.1373321/fullTest; https://doaj.org/toc/1664-3224Test

الوصول الحر: https://doaj.org/article/c7579c996dda4e3597420d4d33bdda20Test

المؤلفون: Junzhang Chen, Yongyu Yang, Yuanhua Liu, Heping Kan

المصدر: World Journal of Surgical Oncology, Vol 19, Iss 1, Pp 1-11 (2021)

مصطلحات موضوعية: Pancreatic neuroendocrine tumor (pNETs), Surveillance, epidemiology, end results (SEER) database, Surgical resection, Prognostic factor, Enucleation, Surgery, RD1-811, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Objective To investigate the prognostic factors of patients with pancreatic neuroendocrine tumor (pNETs) after surgical resection, and to analyze the value of enucleation for pNETs without distant metastasis that are well-differentiated (G1) and have a diameter ≤ 4 cm. Methods Data from pNET patients undergoing surgical resection between 2004 and 2017 were collected from the Surveillance, Epidemiology, and End Results (SEER) database. Kaplan–Meier analysis and log-rank testing were used for the survival comparisons. Adjusted HRs with 95% CIs were calculated using univariate and multivariate Cox regression models to estimate the prognostic factors. P

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1477-7819Test

الوصول الحر: https://doaj.org/article/4e1a015530f14720842f425556e82463Test

المؤلفون: Yongyu Yang, Wang Xiao, Ruoqi Liu, Lei Gao, Junzhang Chen, Heping Kan

المصدر: Journal of Immunology Research, Vol 2022 (2022)

مصطلحات موضوعية: Immunologic diseases. Allergy, RC581-607

الوصف: Background. Lamin family members play crucial roles in promoting oncogenesis and cancer development. The values of lamin family in predicting prognosis and immunotherapy response remain largely unclarified. Our research is aimed at comprehensively estimating the clinical significance of lamin family in hepatocellular carcinoma and constructing a novel lamin family-based signature to predict prognosis and guide the precise immunotherapy. Methods. The expression features and prognostic value of LMNA, LMNB1, and LMNB2 were explored in the TCGA and GEO databases. The biological functions of LMNB1 and LMNB2 were validated by in vitro assays. A lamin family-based signature was built using the TCGA training set. The TCGA test set, entire TCGA set, and GSE14520 set were used to validate its predictive power. Univariate and multivariate analyses were performed to evaluate the independence of the lamin family-based signature from other clinicopathological characteristics. A nomogram was constructed using the lamin family-based signature and TNM stage. The associations of this signature with molecular pathways, clinical characteristics, immune cell infiltration, and immunotherapy response were analyzed. Results. Lamin family members were upregulated in HCC. Upregulation of LMNB1 and LMNB2 promoted HCC proliferation, migration, and invasion. The predictive signature was initially established based on LMNB1 and LMNB2 which could effectively identify differences in overall survival, immune cell infiltration, and clinicopathological characteristics of high- and low-risk patients. The nomogram showed high prognostic predictive accuracy. Importantly, the lamin family-based signature was correlated with immune suppression and expression of immune checkpoint molecules. Conclusions. The lamin family-based signature is a robust biomarker to predict overall survival and immunotherapy response in HCC. High-risk score patients have a poorer overall survival and might be more sensitive to immunotherapy. This signature may contribute to improving individualized prognosis prediction and precision immunotherapy for HCC patients.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2314-7156Test

الوصول الحر: https://doaj.org/article/c0a1a3b7cdaf41e088bec01a6d175501Test

المؤلفون: Junzhang Chen, Mengyao Lyu, Xing Wang, Xiangzhi Bai, Chao Yang, Miaoming Liu, Fugen Zhou

المصدر: IEEE Access, Vol 7, Pp 84182-84196 (2019)

مصطلحات موضوعية: Feature extraction, image registration, interest point detection, Laplacian of Gaussian filter, Electrical engineering. Electronics. Nuclear engineering, TK1-9971

الوصف: Interest point detection has been widely used in image analysis applications. However, some interest points, including small structures and large angle corners, could not be effectively extracted. This paper proposes a limiting form of median Laplacian of Gaussian (LMLG) filter, which combines the superiority of the traditional Laplacian of Gaussian (LoG) filter and a limiting form of the weighted median LoG filter. A detector is also proposed based on the LMLG filter. The LMLG filter aims to improve the detection of LoG-based methods for interest points, especially small structures and large angle corners. Also, it could detect blobs, edges, and local structures. We conduct the repeatability and discrimination experiments on the Oxford dataset. Moreover, we conduct the recall rate experiment on the DTU dataset. The experiments show that the proposed method achieves comparable performance with state-of-the-art methods. In order to verify the utility of the LMLG detector, we carry out a series of interest point detector-based applications: face recognition, infrared-visible image registration, and image classification. The results demonstrate that the LMLG detector performs better than the nine detectors in face recognition. The LMLG detector outperforms the nine detectors and Hrkać's, Han's and Liu's methods in infrared-visible image registration. Our method also gives a comparable result on image classification. The source code of the proposed LMLG detector is made publicly available at https://github.com/chenjzBUAA/LMLG-detectorTest.

وصف الملف: electronic resource

العلاقة: https://ieeexplore.ieee.org/document/8743372Test/; https://doaj.org/toc/2169-3536Test

الوصول الحر: https://doaj.org/article/bc4e96b21fba4edda7af846160d4de7bTest

المؤلفون: Tang, Qiang, Junxin Song, Yile Chen, Zheng, Liang, Junzhang Chen

الإتاحة: https://doi.org/10.13140/rg.2.2.28794.91844Test

المؤلفون: Junzhang Chen

المصدر: Formosa Journal of Sustainable Research; Vol. 1 No. 3 (2022): August 2022; 521-528 ; 2830-6228 ; 10.55927/fjsr.v1i3

مصطلحات موضوعية: Ancient villages, Historical Location, Node Space, The Integration of Vision, View Control Value, Intelligibility

الوصف: Although people can walk out of Cuiwei village, they are always in a confused state, and it is difficult to grasp the spatial form of the whole area. Taking Cuiwei village in Qianshan, Zhuhai as an example, based on the theory of spatial syntax, this paper analyzes the integration degree of ancient village vision and the spatial characteristics of historical location, the understandability degree and integration degree of village vision, the control value of vision and the spatial characteristics of historical location, and puts forward relevant suggestions for the future renewal of villages.

وصف الملف: application/pdf

العلاقة: https://journal.formosapublisher.org/index.php/fjsr/article/view/889/895Test; https://journal.formosapublisher.org/index.php/fjsr/article/view/889Test

الإتاحة: https://doi.org/10.55927/fjsr.v1i3.889Test
https://doi.org/10.55927/fjsr.v1i3Test
https://journal.formosapublisher.org/index.php/fjsr/article/view/889Test

المؤلفون: Junzhang Chen

المصدر: Formosa Journal of Sustainable Research; Vol. 1 No. 3 (2022): August 2022; 479-498 ; 2830-6228 ; 10.55927/fjsr.v1i3

مصطلحات موضوعية: Cuiwei village, spatial syntax, inner and outer streets, historical villages

الوصف: Based on the Cuiwei historical village in Qianshan District of Zhuhai, this study studies the street space inside and outside the village block. With the help of Depthmap and SPSS software, the complex Street axis space is quantitatively analyzed, including the analysis of synergy, spatial form and vitality, pedestrian interface and symbiosis, traffic flow correlation, topological depth and street spatial correlation. Draw relevant conclusions according to the analysis results. In order to provide reference and reference for government management personnel.

وصف الملف: application/pdf

العلاقة: https://journal.formosapublisher.org/index.php/fjsr/article/view/890/887Test; https://journal.formosapublisher.org/index.php/fjsr/article/view/890Test

الإتاحة: https://doi.org/10.55927/fjsr.v1i3.890Test
https://doi.org/10.55927/fjsr.v1i3Test
https://journal.formosapublisher.org/index.php/fjsr/article/view/890Test

المؤلفون: Junzhang Chen

المصدر: Formosa Journal of Sustainable Research; Vol. 1 No. 3 (2022): August 2022; 499-508 ; 2830-6228 ; 10.55927/fjsr.v1i3

مصطلحات موضوعية: Cuiwei Village, Business Type, Three Kings Temple, Webster Mansion, Spatial Characteristics

الوصف: This study takes Cuiwei village, located in Qianshan District of Zhuhai City, as an example. First, it analyzes the human flow activities and activity size of village road a, the visual agglomeration degree and the spatial characteristics of historical sections. Secondly, select the most representative traditional buildings in the village, namely Sanwang temple and Wechsler mansion. When r=n and radius r=3, observe the changes of two parameters of visual integration and visual depth of the two historical buildings. And with the help of Baidu time machine photos of different periods to observe and record the changes of stores and human behavior characteristics. The results show that at this stage, the coordination of villages is poor, people's recognition in the village space is weak, people can't use local perception to reflect the overall space, there is an obvious interface between the inside and outside of the village, the isolation state is obvious, the local intelligibility is good, but the global intelligibility is poor.

وصف الملف: application/pdf

العلاقة: https://journal.formosapublisher.org/index.php/fjsr/article/view/887/888Test; https://journal.formosapublisher.org/index.php/fjsr/article/view/887Test

الإتاحة: https://doi.org/10.55927/fjsr.v1i3.887Test
https://doi.org/10.55927/fjsr.v1i3Test
https://journal.formosapublisher.org/index.php/fjsr/article/view/887Test

المؤلفون: Junzhang Chen, Yile Chen, Liang Zheng

المصدر: Formosa Journal of Sustainable Research; Vol. 1 No. 3 (2022): August 2022; 529-540 ; 2830-6228 ; 10.55927/fjsr.v1i3

مصطلحات موضوعية: Urban construction, landscape architecture planning and design, people, living space

الوصف: This paper discusses and analyzes the current situation of domestic urban landscape architecture planning and design, and takes the Yuanming new garden, a typical modern landscape architecture in Zhuhai, as an example. Based on the bibliometric analysis and the fund research analysis of Wanfang Data Kehui platform, this paper combs the frontier and development trend of the current landscape architecture planning and design research. Secondly, through the field investigation of Zhuhai Yuanming new garden and the visual analysis of the data platform, this paper discusses the current situation, problems in planning practice, and suggestions to be improved.

وصف الملف: application/pdf

العلاقة: https://journal.formosapublisher.org/index.php/fjsr/article/view/821/901Test; https://journal.formosapublisher.org/index.php/fjsr/article/view/821Test

الإتاحة: https://doi.org/10.55927/fjsr.v1i3.821Test
https://doi.org/10.55927/fjsr.v1i3Test
https://journal.formosapublisher.org/index.php/fjsr/article/view/821Test

المؤلفون: Junzhang Chen, Chen, Yile, Zheng, Liang

المصدر: Formosa Journal of Sustainable Research; Vol. 1 No. 2 (2022): July 2022; 231-244 ; 2830-6228 ; 10.55927/fjsr.v1i2

مصطلحات موضوعية: Cantonese traditional village, Cuiwei village, Architectural heritage protection

الوصف: With the rapid development of China's economy, the urbanization process is gradually accelerating, and there are a large number of traditional village spaces are abandoned and compressed, which has greatly changed the original pattern of ancient villages. Cuiwei village is an example of Zhuhai's typical Lingnan Cantonese traditional village evolving into a village in the city, which has experienced many changes in spatial pattern. This paper adopts the method of qualitative investigation to conduct a detailed investigation and analysis of the types of historical buildings in Cuiwei village, and focuses on the impact of historical buildings on people's spatial feelings. It puts forward planning suggestions to provide reference for the follow-up protection of the architectural heritage of Cantonese traditional villages.

وصف الملف: application/pdf

العلاقة: https://journal.formosapublisher.org/index.php/fjsr/article/view/796/575Test; https://journal.formosapublisher.org/index.php/fjsr/article/view/796Test

الإتاحة: https://doi.org/10.55927/fjsr.v1i2.796Test
https://doi.org/10.55927/fjsr.v1i2Test
https://journal.formosapublisher.org/index.php/fjsr/article/view/796Test