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1دورية أكاديمية
المؤلفون: Namsuk Kim, Yan Li, Ri Yu, Hyo-Shin Kwon, Anji Song, Mi-Hee Jun, Jin-Young Jeong, Ji Hyun Lee, Hyun-Ho Lim, Mi-Jin Kim, Jung-Woong Kim, Won-Jong Oh
المصدر: eLife, Vol 13 (2024)
مصطلحات موضوعية: axon guidance, Semaphorin 3E, Plexin-D1, Mtss1, basal ganglia development, Medicine, Science, Biology (General), QH301-705.5
الوصف: Axon guidance molecules are critical for neuronal pathfinding because they regulate directionality and growth pace during nervous system development. However, the molecular mechanisms coordinating proper axonal extension and turning are poorly understood. Here, metastasis suppressor 1 (Mtss1), a membrane protrusion protein, ensured axonal extension while sensitizing axons to the Semaphorin 3E (Sema3E)-Plexin-D1 repulsive cue. Sema3E-Plexin-D1 signaling enhanced Mtss1 expression in projecting striatonigral neurons. Mtss1 localized to the neurite axonal side and regulated neurite outgrowth in cultured neurons. Mtss1 also aided Plexin-D1 trafficking to the growth cone, where it signaled a repulsive cue to Sema3E. Mtss1 ablation reduced neurite extension and growth cone collapse in cultured neurons. Mtss1-knockout mice exhibited fewer striatonigral projections and irregular axonal routes, and these defects were recapitulated in Plxnd1- or Sema3e-knockout mice. These findings demonstrate that repulsive axon guidance activates an exquisite autoregulatory program coordinating both axonal extension and steering during neuronal pathfinding.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Jun Hyung Park, Seungwoo Hong, Ok-Hyeon Kim, Chul-Hong Kim, Jinho Kim, Jung-Woong Kim, Sungguan Hong, Hyun Jung Lee
المصدر: Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
الوصف: Abstract Microplastics (MPs) are now a global issue due to increased plastic production and use. Recently, various studies have been performed in response to the human health risk assessment. However, these studies have focused on spherical MPs, which have smooth edges and a spherical shape and account for less than 1% of MPs in nature. Unfortunately, studies on fragment-type MPs are very limited and remain in the initial stages. In this study, we studied the effect that 16.4 µm fragment type polypropylene (PP) MPs, which have an irregular shape and sharp edges and form naturally in the environment, had on breast cancer. The detrimental effects of PPMPs on breast cancer metastasis were examined. Here, 1.6 mg/ml of PPMP, which does not induce cytotoxicity in MDA-MB-231, was used, and at this concentration, PPMP did not induce morphological changes or cellular migrating in the MDA-MB-231 and MCF-7 cells. However, PPMP incubation for 24 hours in the MDA-MB-231 cells significantly altered the level of cell cycle-related transcripts in an RNA-seq analysis. When confirmed by qRT-PCR, the gene expression of TMBIM6, AP2M1, and PTP4A2 was increased, while the transcript level of FTH1 was decreased. Further, secretion of the pro-inflammatory cytokine IL-6 from cancer cells was elevated with the incubation of PPMP for 12 hours. These results suggest that PPMP enhances metastasis-related gene expression and cytokines in breast cancer cells, exacerbating breast cancer metastasis.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2045-2322Test
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3دورية أكاديمية
المؤلفون: Hee-Jin Kwak, Brenda I. Medina-Jiménez, Soon Cheol Park, Jung-Hyeuk Kim, Geon-Hwi Jeong, Mi-Jeong Jeon, Sangil Kim, Jung-Woong Kim, David A. Weisblat, Sung-Jin Cho
المصدر: Cell & Bioscience, Vol 13, Iss 1, Pp 1-15 (2023)
مصطلحات موضوعية: Slit, Robo, Gene duplication, Axon guidance, Eyespot, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5, Biochemistry, QD415-436
الوصف: Abstract Background Slit and Robo are evolutionarily conserved ligand and receptor proteins, respectively, but the number of slit and robo gene paralogs varies across recent bilaterian genomes. Previous studies indicate that this ligand-receptor complex is involved in axon guidance. Given the lack of data regarding Slit/Robo in the Lophotrochozoa compared to Ecdysozoa and Deuterostomia, the present study aims to identify and characterize the expression of Slit/Robo orthologs in leech development. Results We identified one slit (Hau-slit), and two robo genes (Hau-robo1 and Hau-robo2), and characterized their expression spatiotemporally during the development of the glossiphoniid leech Helobdella austinensis. Throughout segmentation and organogenesis, Hau-slit and Hau-robo1 are broadly expressed in complex and roughly complementary patterns in the ventral and dorsal midline, nerve ganglia, foregut, visceral mesoderm and/or endoderm of the crop, rectum and reproductive organs. Before yolk exhaustion, Hau-robo1 is also expressed where the pigmented eye spots will later develop, and Hau-slit is expressed in the area between these future eye spots. In contrast, Hau-robo2 expression is extremely limited, appearing first in the developing pigmented eye spots, and later in the three additional pairs of cryptic eye spots in head region that never develop pigment. Comparing the expression of robo orthologs between H. austinensis and another glossiphoniid leech, Alboglossiphonia lata allows to that robo1 and robo2 operate combinatorially to differentially specify pigmented and cryptic eyespots within the glossiphoniid leeches. Conclusions Our results support a conserved role in neurogenesis, midline formation and eye spot development for Slit/Robo in the Lophotrochozoa, and provide relevant data for evo-devo studies related to nervous system evolution.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2045-3701Test
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4دورية أكاديمية
المؤلفون: Ji Won Lee, Hyejin Mun, Jeong-Hyun Kim, Seungbeom Ko, Young-Kook Kim, Min Ji Shim, Kyungmin Kim, Chul Woong Ho, Hyun Bong Park, Meesun Kim, Chaeyoung Lee, Si Ho Choi, Jung-Woong Kim, Ji-Hoon Jeong, Je-Hyun Yoon, Kyung-Won Min, Tae Gen Son
المصدر: Biology, Vol 12, Iss 12, p 1533 (2023)
مصطلحات موضوعية: low-dose ionizing radiation (LDIR), RNA crosslinking, post-transcriptional gene regulation, Biology (General), QH301-705.5
الوصف: Although ionizing radiation (IR) is widely used for therapeutic and research purposes, studies on low-dose ionizing radiation (LDIR) are limited compared with those on other IR approaches, such as high-dose gamma irradiation and ultraviolet irradiation. High-dose IR affects DNA damage response and nucleotide–protein crosslinking, among other processes; however, the molecular consequences of LDIR have been poorly investigated. Here, we developed a method to profile RNA species crosslinked to an RNA-binding protein, namely, human antigen R (HuR), using LDIR and high-throughput RNA sequencing. The RNA fragments isolated via LDIR-crosslinking and immunoprecipitation sequencing were crosslinked to HuR and protected from RNase-mediated digestion. Upon crosslinking HuR to target mRNAs such as PAX6, ZFP91, NR2F6, and CAND2, the transcripts degraded rapidly in human cell lines. Additionally, PAX6 and NR2F6 downregulation mediated the beneficial effects of LDIR on cell viability. Thus, our approach provides a method for investigating post-transcriptional gene regulation using LDIR.
وصف الملف: electronic resource
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5دورية أكاديمية
المؤلفون: Yong-Hyun Joo, Yeong-Geun Lee, Younghyun Lim, Hoyeon Jeon, In-Gu Lee, Yong-Bin Cho, So-Hee Hong, Eui Ho Kim, Soon Ho Choi, Jung-Woong Kim, Se Chan Kang, Young-Jin Seo
المصدر: Biomedicine & Pharmacotherapy, Vol 155, Iss , Pp 113773- (2022)
مصطلحات موضوعية: Antiviral drug, Influenza A virus, APRG64, Apigenin, Therapeutics. Pharmacology, RM1-950
الوصف: Influenza A virus (IAV) continues to threaten human health. To date, two classes of antiviral drugs have been approved to treat IAV infection, but the continuous emergence of the drug-resistant IAV mutant reinforces the need to develop new antiviral drugs. In this study, we aimed to investigate the anti-IAV activity of an aqueous mixture of Agrimonia pilosa and Galla rhois extracts (APRG64). We demonstrated that APRG64 significantly reduced the IAV-induced cytopathic effect, the transcription/expression of viral proteins, and the production of infectious viral particles. Among nine major components of APRG64, apigenin was identified as the main ingredient responsible for the anti-IAV activity. Interestingly, APRG64 and apigenin inhibited the cell attachment and entry of virus and polymerase activity. Importantly, intranasal administration of APRG64 or apigenin strongly reduced viral loads in the lungs of IAV-infected mice. Furthermore, oral administration of APRG64 significantly reduced the level of viral RNAs and the expression level of pro-inflammatory cytokines in the lungs, which protected mice from IAV-induced mortality. In conclusion, APRG64 could be an attractive antiviral drug to treat IAV infection.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S0753332222011623Test; https://doaj.org/toc/0753-3322Test
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6دورية أكاديمية
المؤلفون: Jung-Woong Kim, Junyeong Yi, Jinhong Park, Ji Hoon Jeong, Jinho Kim, Jihee Won, Seok Chung, Tong-Soo Kim, Jhang Ho Pak
المصدر: Parasites & Vectors, Vol 14, Iss 1, Pp 1-16 (2021)
مصطلحات موضوعية: Clonorchis sinensis infection, Excretory-secretory products, Three-dimensional, Cholangiocyte spheroids, Transcriptomic profiling, Microarray, Infectious and parasitic diseases, RC109-216
الوصف: Abstract Background Biliary tract infection with the carcinogenic human liver fluke, Clonorchis sinensis, provokes chronic inflammation, epithelial hyperplasia, periductal fibrosis, and even cholangiocarcinoma. Complications are proportional to the intensity and duration of the infection. In addition to mechanical irritation of the biliary epithelia from worms, their excretory-secretory products (ESPs) cause chemical irritation, which leads to inflammation, proliferation, and free radical generation. Methods A three-dimensional in vitro cholangiocyte spheroid culture model was established, followed by ESP treatment. This allowed us to examine the intrinsic pathological mechanisms of clonorchiasis via the imitation of prolonged and repetitive in vivo infection. Results Microarray and RNA-Seq analysis revealed that ESP-treated cholangiocyte H69 spheroids displayed global changes in gene expression compared to untreated spheroids. In ESP-treated H69 spheroids, 185 and 63 probes were found to be significantly upregulated and downregulated, respectively, corresponding to 209 genes (p 2). RNA-Seq was performed for the validation of the microarray results, and the gene expression patterns in both transcriptome platforms were well matched for 209 significant genes. Gene ontology analysis demonstrated that differentially expressed genes were mainly classified into immune system processes, the extracellular region, and the extracellular matrix. Among the upregulated genes, four genes (XAF1, TRIM22, CXCL10, and BST2) were selected for confirmation using quantitative RT-PCR, resulting in 100% similar expression patterns in microarray and RNA-Seq. Conclusions These findings broaden our understanding of the pathological pathways of liver fluke-associated hepatobiliary disorders and suggest a novel therapeutic strategy for this infectious cancer. Graphic abstract
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/1756-3305Test
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7دورية أكاديمية
المؤلفون: Mi Jin Kim, Chul-Hong Kim, Mi-Jin An, Ju-Hyun Lee, Geun-Seup Shin, Mina Song, Jung-Woong Kim
المصدر: Animal Cells and Systems, Vol 24, Iss 1, Pp 34-43 (2020)
مصطلحات موضوعية: ethylparaben, apoptosis, cell cycle arrest, human placenta cells, bewo, Medicine (General), R5-920, Biology (General), QH301-705.5
الوصف: Parabens are generally used as preservatives in foods, pharmaceuticals, and various other commercial products. Among them, ethylparaben has weaker estrogenic characteristics than endogenous estrogen. However, growing evidence indicates that ethylparaben has an adverse effect on various human tissues. Here, we investigated whether ethylparaben induces cell death by affecting cell viability, cell proliferation, cell cycle, and apoptosis using the human placenta cell line BeWo. Ethylparaben significantly decreased cell viability in a dose-dependent manner. It caused cell cycle arrest at sub-G1 by reducing the expression of cyclin D1, whereas it decreased the cell proportion at the G0/G1 and S phases. Furthermore, we verified that ethylparaben induces apoptotic cell death by enhancing the activity of Caspase-3. Taken together, our results suggest that ethylparaben exerts cytotoxic effects in human placental BeWo cells via cell cycle arrest and apoptotic pathways.
وصف الملف: electronic resource
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8دورية أكاديمية
المؤلفون: Seobin Yoon, Eui-Hwan Choi, Jung-Woong Kim, Keun P. Kim
المصدر: Experimental and Molecular Medicine, Vol 50, Iss 8, Pp 1-12 (2018)
مصطلحات موضوعية: Medicine, Biochemistry, QD415-436
الوصف: Cell division: Recombination in action Using very high resolution microscopy, Korean researchers are able to visualize meiosis, a two-step division process that ensures that each egg or sperm cell has a single set of chromosomes, in unprecedented detail. After DNA replication and before the first division cycle, pairs of chromosomes align to exchange genetic material, a process known as recombination. This process is facilitated by multi-protein structures called synaptonemal complexes (SCs) that hold the chromosomes together. Keun Kim and colleagues at Chung-Ang University, Seoul, South Korea used structured illumination microscopy to observe SCs during meiosis in mouse sperm-forming cells. They found that the position of replication protein A, a key regulator of recombination, on the SC changes as recombination takes place. These findings suggest that RPA location on SCs could be used to monitor the progression of meiotic recombination.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2092-6413Test
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9دورية أكاديمية
المؤلفون: Ja Young Hahm, Jin Woo Park, Joo-Young Kang, Junyoung Park, Chul-Hong Kim, Ji-Young Kim, Nam-Chul Ha, Jung-Woong Kim, Sang-Beom Seo
المصدر: Cell Reports, Vol 32, Iss 4, Pp 107958- (2020)
مصطلحات موضوعية: UHRF1, PCAF, acetylation, DNA methylation, HDAC1, hemi-methylated DNA, Biology (General), QH301-705.5
الوصف: Summary: UHRF1 is a key regulator in DNA methylation maintenance. It binds histone H3K9me2/3 and hemi-methylated DNA and recruits DNMT1 to DNA replication forks during S phase. However, the regulatory mechanism of hemi-methylated DNA binding activity of UHRF1 remains unknown. In this study, we reveal that acetylation of UHRF1 is regulated by PCAF and HDAC1. We show that UHRF1 acetylation at K490 attenuates its binding affinity to hemi-methylated DNA. We analyze genome-wide DNA methylation and gene-expression patterns using stable cell lines and discover that cells where the endogenous UHRF1 is replaced with an acetyl-mimetic (UHRF1 K490Q) mutant show deficiencies in inherited DNA methylation and show different gene-expression patterns in genes related to cell survival. These results reveal that precise regulation of UHRF1 acetylation is required to maintain DNA methylation during cell division and control cell survival.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S2211124720309396Test; https://doaj.org/toc/2211-1247Test
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10دورية أكاديمية
المؤلفون: Songyi Seo, Koung Li Kim, Yeongju Yeo, Ryul-I Kim, Hayoung Jeong, Jin-Ock Kim, Sun-Hwa Song, Mi-Jin An, Jung-Woong Kim, Hye Kyoung Hong, Min Hee Ham, Se Joon Woo, Jong-Hyuk Sung, Sang Gyu Park, Wonhee Suh
المصدر: Pharmaceutics, Vol 13, Iss 8, p 1308 (2021)
مصطلحات موضوعية: age-related macular degeneration, choroidal neovascularization, cKIT, fully human monoclonal antibody, stem cell factor, Pharmacy and materia medica, RS1-441
الوصف: Stem cell factor (SCF) and its receptor, cKIT, are novel regulators of pathological neovascularization in the eye, which suggests that inhibition of SCF/cKIT signaling may be a novel pharmacological strategy for treating neovascular age-related macular degeneration (AMD). This study evaluated the therapeutic potential of a newly developed fully human monoclonal antibody targeting cKIT, NN2101, in a murine model of neovascular AMD. In hypoxic human endothelial cells, NN2101 substantially inhibited the SCF-induced increase in angiogenesis and activation of the cKIT signaling pathway. In a murine model of neovascular AMD, intravitreal injection of NN2101 substantially inhibited the SCF/cKIT-mediated choroidal neovascularization (CNV), with efficacy comparable to aflibercept, a vascular endothelial growth factor inhibitor. A combined intravitreal injection of NN2101 and aflibercept resulted in an additive therapeutic effect on CNV. NN2101 neither caused ocular toxicity nor interfered with the early retinal vascular development in mice. Ocular pharmacokinetic analysis in rabbits indicated that NN2101 demonstrated a pharmacokinetic profile suitable for intravitreal injection. These findings provide the first evidence of the potential use of the anti-cKIT blocking antibody, NN2101, as an alternative or additive therapeutic for the treatment of neovascular AMD.
وصف الملف: electronic resource
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