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1دورية أكاديمية
المؤلفون: Georg Petschenka, Colleen S. Fei, Juan J. Araya, Susanne Schröder, Barbara N. Timmermann, Anurag A. Agrawal
المصدر: Frontiers in Plant Science, Vol 9 (2018)
مصطلحات موضوعية: monarch butterfly, Na+/K+-ATPase, cardenolide, cardiac glycoside, phytochemical diversity, structure–activity relationship, Plant culture, SB1-1110
الوصف: A major prediction of coevolutionary theory is that plants may target particular herbivores with secondary compounds that are selectively defensive. The highly specialized monarch butterfly (Danaus plexippus) copes well with cardiac glycosides (inhibitors of animal Na+/K+-ATPases) from its milkweed host plants, but selective inhibition of its Na+/K+-ATPase by different compounds has not been previously tested. We applied 17 cardiac glycosides to the D. plexippus-Na+/K+-ATPase and to the more susceptible Na+/K+-ATPases of two non-adapted insects (Euploea core and Schistocerca gregaria). Structural features (e.g., sugar residues) predicted in vitro inhibitory activity and comparison of insect Na+/K+-ATPases revealed that the monarch has evolved a highly resistant enzyme overall. Nonetheless, we found evidence for relative selectivity of individual cardiac glycosides reaching from 4- to 94-fold differences of inhibition between non-adapted Na+/K+-ATPase and D. plexippus-Na+/K+-ATPase. This toxin receptor specificity suggests a mechanism how plants could target herbivores selectively and thus provides a strong basis for pairwise coevolutionary interactions between plants and herbivorous insects.
وصف الملف: electronic resource
العلاقة: https://www.frontiersin.org/article/10.3389/fpls.2018.01424/fullTest; https://doaj.org/toc/1664-462XTest
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المؤلفون: María Isabel Ríos, Catalina Murillo-Cruz, Giselle Tamayo-Castillo, Roberto Avendaño, María Luisa Gómez-Calvo, Max Chavarría, Adrián A. Pinto-Tomás, Juan J. Araya, Bernal Matarrita-Carranza
المصدر: Kérwá
Universidad de Costa Rica
instacron:UCRمصطلحات موضوعية: Wasps, Human pathogen, Microbiology, Streptomyces, Actinobacteria, 03 medical and health sciences, Plasmid, Tandem Mass Spectrometry, Genome mining, Animals, Humans, Candida albicans, Symbiosis, Molecular Biology, Phylogeny, 030304 developmental biology, 0303 health sciences, biology, 030306 microbiology, Nonactin, General Medicine, Antimicrobial, biology.organism_classification, Anti-Bacterial Agents, Hypocreales, Macrotetrolide antibiotics, Streptomyces griseus, Polybia, Bacteria, Chromatography, Liquid
الوصف: Streptomyces symbionts in insects have shown to be a valuable source of new antibiotics. Here, we report the genome sequence and the potential for antibiotic production of ‘‘Streptomyces sp. M54’’, an Actinobacteria associated with the eusocial wasp, Polybia plebeja. The Streptomyces sp. M54 genome is composed of a chromosome (7.96 Mb), and a plasmid (1.91 Kb) and harbors 30 biosynthetic gene clusters for secondary metabolites, of which only one third has been previously characterized. Growth inhibition bioassays show that this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens taphylococcus aureus and Candida albicans. Analyses through TLC-bioautography, LC–MS/MS and NMR allowed the identification of five macrocyclic ionophore antibiotics, with previously reported antibacterial, antitumor and antiviral properties. Phylogenetic analyses placed Streptomyces sp.M54 in a clade of other host-associated strains taxonomically related to Streptomyces griseus. Pangenomic and ANI analyses confirm the identity of one of its closest relatives as Streptomyces sp. LaPpAH-199, a strain isolated from an ant-plant symbiosis in Africa. In summary, our results suggest an insect-microbe association in distant geographic areas and showcase the potential of Streptomyces sp. M54 and related strains for the discovery of novel antibiotics UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA)
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0dee12108a648f5078a8534799effb2dTest
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المؤلفون: Sebastián Gutierrez-Vega, Marcelo González, Barbara Valderrama-Gutiérrez, Bernel Ortega-Contreras, Juan J. Araya, Karin Lagos-SanMartin, Enrique Guzmán-Gutiérrez, Daniela Mennickent, Andrés Rodríguez, Marcelo Farías-Jofré
المصدر: Placenta. 103
مصطلحات موضوعية: 0301 basic medicine, Adult, Thyroid Hormones, endocrine system diseases, Thyroid Gland, Thyroid Function Tests, Machine learning, computer.software_genre, Correlation, Machine Learning, 03 medical and health sciences, 0302 clinical medicine, Second trimester, Pregnancy, Risk Factors, medicine, Humans, Principal Component Analysis, 030219 obstetrics & reproductive medicine, business.industry, Thyroid, nutritional and metabolic diseases, Obstetrics and Gynecology, medicine.disease, Thyroid abnormalities, female genital diseases and pregnancy complications, Gestational diabetes, Diabetes, Gestational, Pregnancy Trimester, First, 030104 developmental biology, medicine.anatomical_structure, Reproductive Medicine, Thyroid hormones, Pregnancy Trimester, Second, Female, Artificial intelligence, business, computer, Maternal Serum Screening Tests, Developmental Biology
الوصف: There is evidence about a possible relationship between thyroid abnormalities and gestational diabetes mellitus (GDM). However, there is still no conclusive data on this dependence, since no strong correlation has been proved. In this work, we used machine learning to determine whether there is a correlation between maternal thyroid profile in first and second trimester of pregnancy and GDM. Using principal component analysis, it was possible to find an evident correlation between both, which could be used as a complement for a more sensitive GDM diagnosis.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::122d4a5ce3ed12402638b6d28a02a455Test
https://pubmed.ncbi.nlm.nih.gov/33099203Test -
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المؤلفون: Andreas Kaufmann, Gabriele Köllisch, Alfonso J. García-Piñeres, Wibke E. Diederich, Stefan Bauer, Juan J. Araya, Kerstin Mark, Francisco A. Venegas, Max Chavarría
المصدر: Scientific Reports, vol.9(13661), pp.1-17
Kérwá
Universidad de Costa Rica
instacron:UCR
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-17 (2019)مصطلحات موضوعية: Models, Molecular, 0301 basic medicine, Indoles, Protein Conformation, THP-1 Cells, Interleukin-1beta, lcsh:Medicine, Peripheral blood mononuclear cell, Article, Immunostimulatory, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Immune system, Target identification, Animals, Humans, Secretion, lcsh:Science, Transcriptomics, Monocytes and macrophages, Multidisciplinary, biology, Assay systems, Interleukin-6, Chemistry, Chromobacterium, lcsh:R, Bacterial, TLR7, TLR8, biology.organism_classification, Cell biology, Imidazoquinoline, HEK293 Cells, RAW 264.7 Cells, 030104 developmental biology, Gene Expression Regulation, Toll-Like Receptor 8, Cell culture, 030220 oncology & carcinogenesis, Leukocytes, Mononuclear, lcsh:Q, Chromobacterium violaceum, Signal Transduction, Violacein
الوصف: Violacein, an indole-derived, purple-colored natural pigment isolated from Chromobacterium violaceum has shown multiple biological activities. In this work, we studied the effect of violacein in different immune cell lines, namely THP-1, MonoMac 6, ANA-1, Raw 264.7 cells, as well as in human peripheral blood mononuclear cells (PBMCs). A stimulation of TNF-α production was observed in murine macrophages (ANA-1 and Raw 264.7), and in PBMCs, IL-6 and IL-1β secretion was detected. We obtained evidence of the molecular mechanism of activation by determining the mRNA expression pattern upon treatment with violacein in Raw 264.7 cells. Incubation with violacein caused activation of pathways related with an immune and inflammatory response. Our data utilizing TLR-transfected HEK-293 cells indicate that violacein activates the human TLR8 (hTLR8) receptor signaling pathway and not human TLR7 (hTLR7). Furthermore, we found that the immunostimulatory effect of violacein in PBMCs could be suppressed by the specific hTLR8 antagonist, CU-CPT9a. Finally, we studied the interaction of hTLR8 with violacein in silico and obtained evidence that violacein could bind to hTLR8 in a similar fashion to imidazoquinoline compounds. Therefore, our results indicate that violacein may have some potential in contributing to future immune therapy strategies. Universidad de Costa Rica/[801-B2-519]/UCR/Costa Rica Ministerio de Ciencia, Tecnología y Telecomunicaciones/[FI-497-11]/MICITT/Costa Rica Ministerio de Ciencia, Tecnología y Telecomunicaciones/[DFG-TR84]/MICITT/Costa Rica Ministerio de Ciencia, Tecnología y Telecomunicaciones/[DFG-KFO325]/MICITT/Costa Rica UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Química UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigaciones en Productos Naturales (CIPRONA) UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM) UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones Farmacéuticas (INIFAR)
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17c8b8424799dcfa1237231a5b8bb2a2Test
https://doi.org/10.1038/s41598-019-50038-xTest -
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المصدر: Microchemical Journal. 134:164-172
مصطلحات موضوعية: Materials science, Pixel, 010401 analytical chemistry, Analytical chemistry, Hyperspectral imaging, 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, Least squares, 0104 chemical sciences, Analytical Chemistry, Search engine, symbols.namesake, Fourier transform, symbols, Calibration, Fiber, 0210 nano-technology, Biological system, Spectroscopy, Microscale chemistry
الوصف: The surface of lignocellulosic pulp fibers was studied at microscopic level using Fourier transformed infrared imaging to obtain information regarding pattern distribution and concentration of the lignocellulosic components. As each pixel of the hyperspectral image contains a mixture of spectral information of all of the components present within, multivariate curve resolution-alternating least squares (MCR-ALS) analysis was performed to recover the pure spectra of the main components of lignocellulosic materials and determine their distribution on the surface of the fiber. Although MCR-ALS is not a quantitative analysis, the relative concentration profile obtained by MCR-ALS can be used to obtain real pixel-to-pixel quantitative information of the components. Some strategies have been described to transform the values obtained from MCR-ALS into real concentration units; however, they have been applied mostly to artificial mixtures, using available reference materials to create calibration models. In this work, two simple strategies are proposed to estimate single pixel concentration when no reference material is available. These strategies can be especially relevant for microscale quantitative analysis of natural, complex and heterogeneous samples.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::1ddd16a1a35bbf02ba2f42d069487f10Test
https://doi.org/10.1016Test/j .microc.2017.05.019 -
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المؤلفون: Cristina Muñoz, María de los Ángeles Sepúlveda, Juan J. Araya, Manuel F. Casanova, Daniel Wasner, Pascal Boeckx, Samuel Bodé, Ben Colpaert, Sebastian Doetterl, Erick Zagal, Marcela Hidalgo
المصدر: Geoderma Regional, 25
GEODERMA REGIONALمصطلحات موضوعية: DYNAMICS, Inceptisol, Soil test, Soil Science, chemistry.chemical_element, Soil science, 010501 environmental sciences, 01 natural sciences, NIR SPECTROSCOPY, Carbon cycle, DELTA-C-13, Near-infrared spectroscopy, Andisols, Carbon dynamics, Partial least squares regression, SOC, Water content, SCALE, ddc:910, 0105 earth and related environmental sciences, 04 agricultural and veterinary sciences, Vegetation, Inceptisols, Alfisols, REFLECTANCE SPECTROSCOPY, CLIMATE, Carbon isotope abundance, ORGANIC-MATTER, VARIABILITY, chemistry, Mollisols, Isotopes of carbon, Earth and Environmental Sciences, δ13C, 040103 agronomy & agriculture, delta C-13, 0401 agriculture, forestry, and fisheries, Environmental science, Isotope ratio mass spectrometer, Partial least-squares regression, Random forest, Carbon
الوصف: The role of soil in the global carbon cycle and carbon–climate feedback mechanisms has attracted considerable interest in recent decades. Consequently, development of simple, rapid, and inexpensive methods to support the studies on carbon dynamics in soil is of interest. Near-infrared spectroscopy (NIRS) has emerged as a rapid and cost-effective method for measurements of soil properties. The aim of this study was to develop and validate a predictive model for δ13C values using NIRS in various soil profiles across Chile. Eleven sites were selected in the range of 30° to 50° S. These sites represent different soil moisture and soil temperature regimes, clay mineralogies, parent materials, and climates; in addition, they have prairie vegetation and contain C3-type vegetation. Air-dried soil samples were scanned in the NIR range at a resolution of 4 cm−1. The carbon isotopic composition, expressed as δ13C relative to the Vienna Pee Dee Belemnite standard, was analysed using an elemental analyser–isotope ratio mass spectrometer system. A prediction model for δ13C values based on NIRS data was developed through a partial least-squares regression (PLS) model using ten latent variables. A second model was generated using a random forest (RF) approach. The model performances were acceptable. The RF model provided the best results. The values of the root mean square error of prediction for the validation runs for δ13C obtained using the PLS and RF models were 1.38‰, and 1.15‰, respectively. These model performances indicate that NIRS can be used to predict δ13C for the selected dataset. The results of this study support the use of NIRS as a predictive method in soil analyses and as a nondestructive waste-free method for studies on carbon dynamics in soil.
Geoderma Regional, 25وصف الملف: application/pdf; application/application/pdf
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc6477446996878bb05b8bcd3abace43Test
https://doi.org/10.1016Test/j .geodrs.2021.e00397 -
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المؤلفون: Eduardo Troncoso, Juan J. Araya, Carlos Peña-Farfal, Juanita Freer, Nelson P. Barrera, Cristian Arévalo, Rosario Castillo, Pamela A. Naulin
المصدر: BioEnergy Research. 10:714-727
مصطلحات موضوعية: 0106 biological sciences, Chromatography, biology, Renewable Energy, Sustainability and the Environment, Chemistry, 02 engineering and technology, 021001 nanoscience & nanotechnology, biology.organism_classification, 01 natural sciences, Hydrolysis, chemistry.chemical_compound, 010608 biotechnology, Eucalyptus globulus, Yield (chemistry), Enzymatic hydrolysis, Ultrastructure, Organic chemistry, Acid hydrolysis, Cellulose, 0210 nano-technology, Agronomy and Crop Science, Secondary cell wall, Energy (miscellaneous)
الوصف: Dilute acid hydrolysis (DAH) and auto-hydrolysis (AU) have demonstrated to be optimal pre-treatments for the generation of biofuels from wood. Recent studies have highlighted the importance of ensuring the accessibility of cellulose enzymes during the enzymatic hydrolysis (EH) of pre-treated materials. In this work, the microscopic and nanoscopic structures of Eucalyptus globulus samples pre-treated by AU and DAH were evaluated by different techniques to understand the effect of the ultrastructure of samples on the enzymatic conversion and cellulose accessibility for bioethanol production. Microscopic techniques revealed changes in the physical characteristics of pre-treated fibers, coalescence at microscopic level, and differences in the chemical distribution of lignocellulosic components depending on the severity and type of pre-treatment. The atomic force microscopy-based nanoscopic study of samples showed differences in the effect of the pre-treatments on the ultrastructure of samples, with DAH pre-treatment producing major changes in the secondary cell wall with respect to AU samples at comparable severities, and a positive effect of the DAH ultrastructure changes to increase the EH yield.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::c0caf08b03392fec3d39ccc93b26c1d4Test
https://doi.org/10.1007/s12155-017-9833-8Test -
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المؤلفون: Carlos O. Morales, Clara Steinbrueck, Alfonso J. García-Piñeres, Juan J. Araya, Nohelia Mora-Ugalde, Randall Loiaza
المصدر: Phytochemistry Letters, vol 29, pp. 190-194
Kérwá
Universidad de Costa Rica
instacron:UCRمصطلحات موضوعية: Sulfated withanolides, medicine.drug_class, Cytotoxicity, Acnistus arborescens, Plant Science, 01 natural sciences, Biochemistry, Anti-inflammatory, chemistry.chemical_compound, Sulfation, Ic50 values, medicine, Bioassay, Anti-inlammatory, biology, Traditional medicine, 010405 organic chemistry, biology.organism_classification, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, chemistry, Withaferin A, Agronomy and Crop Science, Solanaceae, Biotechnology
الوصف: Bioassay-guided fractionation of Acnistus arborescens (Solanaceae) organic extract afforded two anti-inflammatory withanolides: 2,3-Dihydro-3β-O-sulfate withacnistin (1) and 2,3-Dihydro-3β-O-sulfate withaferin A (2) showing IC50 values of 1.0 ± 0.1 and 14 ± 1 μM respectively in a LPS-stimulated RAW 264.7 murine macrophage assay. These compounds were also evaluated against two selected cancer cell lines showing significant cytotoxicity. Although there are several reports dealing with the chemistry of this species, we report for the first time the isolation of O-sulfated withanolides. Universidad de Costa Rica/[ 801-B8-511]/UCR/Costa Rica UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM) UCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biología
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::633278953b26ab0a27c5d3b6ee74fa75Test
https://www.sciencedirect.com/science/article/pii/S1874390018302556Test#! -
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المصدر: Chemometrics and Intelligent Laboratory Systems. 195:103890
مصطلحات موضوعية: 0303 health sciences, Pixel, Dynamic range, Process Chemistry and Technology, 010401 analytical chemistry, Iterative reconstruction, 01 natural sciences, Sample (graphics), 0104 chemical sciences, Computer Science Applications, Analytical Chemistry, 03 medical and health sciences, symbols.namesake, Fourier transform, Partial least squares regression, Range (statistics), Calibration, symbols, Biological system, Spectroscopy, Software, 030304 developmental biology, Mathematics
الوصف: Pretreated lignocellulosic fibers were used as a case study to compare two chemometric methods for the quantification of chemical components in Fourier transformed infrared (FT-IR) images. Partial least squares (PLS) and multivariate curve resolution with alternating least squares (MCR-ALS) methods were applied to the images to quantify glucans, lignin and hemicellulose content. The main problem for calibration in samples from natural origin is to obtain proper reference material for pixel to pixel quantification. Furthermore, chemical components in wood experience changes after different pretreatment conditions; therefore commercially available reference material may not have the same identity of the components present in the sample. Concentration information of bulk samples obtained by wet chemistry methods, along with the median spectrum of whole images, was used as an alternative for PLS calibration in this scenario. Results show that both methods provided similar spatial distribution for lignin and hemicellulose in the concentration maps, but image reconstruction of glucans shows differences in distribution between the two methods. PLS models used to quantify pixels in an image were previously validated through the prediction of global concentration of samples, using the median spectrum of different images (RMSEP = 1.3% for glucans, 1.0% for lignin and 0.9% for hemicelluloses); The range of pixel concentration predicted in a single image was too narrow possibly due to the lack of a calibration set with a wider dynamic range. Concentration maps obtained with MCR-ALS were satisfactory and the range of concentration for pixels was more consistent with what would be expected. A quantification approach that does not need a calibration set was used to transform concentration profiles into real concentration units for pixels. Therefore MCR-ALS was a more suitable method for quantification in this specific case study.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::e406e56a9d469f8a0a65643063f14e5bTest
https://doi.org/10.1016Test/j .chemolab.2019.103890 -
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المؤلفون: Georg Petschenka, Colleen S. Fei, Juan J. Araya, Susanne Schröder, Barbara N. Timmermann, Anurag A. Agrawal
مصطلحات موضوعية: Botany, Plant Biology, Plant Systematics and Taxonomy, Plant Cell and Molecular Biology, Plant Developmental and Reproductive Biology, Plant Pathology, Plant Physiology, Plant Biology not elsewhere classified, monarch butterfly, Na+/K+-ATPase, cardenolide, cardiac glycoside, phytochemical diversity, structure–activity relationship, toxin–receptor interaction, resistance
الوصف: A major prediction of coevolutionary theory is that plants may target particular herbivores with secondary compounds that are selectively defensive. The highly specialized monarch butterfly (Danaus plexippus) copes well with cardiac glycosides (inhibitors of animal Na + /K + -ATPases) from its milkweed host plants, but selective inhibition of its Na + /K + -ATPase by different compounds has not been previously tested. We applied 17 cardiac glycosides to the D. plexippus-Na + /K + -ATPase and to the more susceptible Na + /K + -ATPases of two non-adapted insects (Euploea core and Schistocerca gregaria). Structural features (e.g., sugar residues) predicted in vitro inhibitory activity and comparison of insect Na + /K + -ATPases revealed that the monarch has evolved a highly resistant enzyme overall. Nonetheless, we found evidence for relative selectivity of individual cardiac glycosides reaching from 4- to 94-fold differences of inhibition between non-adapted Na + /K + -ATPase and D. plexippus-Na + /K + -ATPase. This toxin receptor specificity suggests a mechanism how plants could target herbivores selectively and thus provides a strong basis for pairwise coevolutionary interactions between plants and herbivorous insects.
الإتاحة: https://doi.org/10.3389/fpls.2018.01424.s001Test
https://figshare.com/articles/dataset/Data_Sheet_1_Relative_Selectivity_of_Plant_Cardenolides_for_Na_K_-ATPases_From_the_Monarch_Butterfly_and_Non-resistant_Insects_doc/7144919Test
النص الكامل