المؤلفون: Ji‐Won Seo, Seongin Jo, Young Sung Jung, Mohammad‐Al Mijan, Joy Cha, Seungpyo Hong, Sanguine Byun, Tae‐Gyu Lim

المصدر: BioFactors (Oxford, England)REFERENCES.

مصطلحات موضوعية: Clinical Biochemistry, Molecular Medicine, General Medicine, Biochemistry

الوصف: Rosa gallica has been previously reported to display anti-inflammatory, anti-oxidative, and anti-skin wrinkle activities. However, the effect of Rosa gallica on skin hydration and its active components are largely unknown. Herein, we aimed to investigate the skin hydration effect of rose petal extract (RPE) in humans and elucidate the underlying molecular mechanism. A double-blinded clinical study was performed to investigate the effect of RPE on skin hydration. Stratum corneum moisture analysis demonstrated that RPE treatment significantly improved hydration levels in human skin. Furthermore, HAS2 and hyaluronic acid levels were notably increased by RPE in keratinocytes and 3D human skin equivalent model. By comparing the modulatory effect on HAS2 expression, cyanidin-3,5-O-diglucoside (CDG) was identified as the most potent compound in RPE likely responsible for skin hydration. The kinase activity of GLK, an upstream regulator of MAPK signaling, was increased by CDG in a dose-dependent manner. Importantly, silencing GLK reversed CDG-mediated HAS2 upregulation, further supporting the involvement of GLK in the CDG-mediated effects. Binding of CDG to GLK was confirmed by pull-down assay and computer modeling. These findings suggest that RPE and its active component CDG increases skin hydration by upregulating HAS2 expression through modulating the GLK-MAP2K-MAPK signaling pathway.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a082e267513f5d76d757cac09887ee5bTest
https://pubmed.ncbi.nlm.nih.gov/36573713Test

المؤلفون: Young Jin Ko, Joy Cha, Wu-Young Jeong, Myeong-Eun Lee, Byeong-Hyeon Cho, Bhardwaj Nisha, Hyun Jin Jeong, Sung Eun Park, Sung Ok Han

المصدر: Bioresource Technology. 354:127171

مصطلحات موضوعية: 2-Propanol, Corynebacterium glutamicum, Environmental Engineering, Metabolic Engineering, Renewable Energy, Sustainability and the Environment, Fermentation, Succinic Acid, Bioengineering, General Medicine, Waste Management and Disposal

الوصف: Isopropanol is a commodity chemical widely used as a biofuel, fuel additive, rubbing alcohol and intermediate in various fields. Here, an engineered Corynebacterium glutamicum overproducing isopropanol was developed. To our knowledge, despite a representative industrial host to produce valuable chemicals, the high-level production of isopropanol in C. glutamicum has never been reported. First, the problem of the inability to produce isopropanol was solved by finding a key factor in its metabolism. The consolidation and modular optimization of synthetic bypasses including succinate and mevalonate bypasses enhanced isopropanol production. Flux redistribution of central metabolism significantly directed the carbon flux toward isopropanol biosynthesis. The final engineered strain produced 10.25 ± 1.12 g/L isopropanol in two-stage fed-batch fermentation with an optimized gas stripping, which is the highest titer, yield and productivity in C. glutamicum. These strategies could be useful for the high-level production of isopropanol in C. glutamicum.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6da35f465bdc919348f5e40406c4fc24Test
https://doi.org/10.1016/j.biortech.2022.127171Test

المؤلفون: Rupsha Mondal, Chayan Banerjee, Sumangal Nandy, Moumita Roy, Joy Chakraborty

المصدر: Cell & Bioscience, Vol 13, Iss 1, Pp 1-18 (2023)

مصطلحات موضوعية: Dopamine toxicity, Mitochondrial fragmentation, Calcineurin, Parkinson’s disease, L-DOPA therapy, Dendritic spine, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5, Biochemistry, QD415-436

الوصف: Abstract Background Parkinson’s disease (PD), a highly prevalent neuro-motor disorder is caused due to progressive loss of dopaminergic (DAergic) neurons at substantia nigra region of brain. This leads to depleted dopamine (DA) content at striatum, thus affecting the fine tuning of basal ganglia. In patients, this imbalance is manifested by akinesia, catalepsy and tremor. PD associated behavioral dysfunctions are frequently mitigated by l-DOPA (LD) therapy, a precursor for DA synthesis. Due to progressive neurodegeneration, LD eventually loses applicability in PD. Although DA is cytotoxic, it is unclear whether LD therapy can accelerate PD progression or not. LD itself does not lead to neurodegeneration in vivo, but previous reports demonstrate that LD treatment mediated excess DA can potentiate neurotoxicity when PD associated genetic or epigenetic aberrations are involved. So, minimizing DA toxicity during the therapy is an absolute necessity to halt or slowdown PD progression. The two major contributing factors associated with DA toxicity are: degradation by Monoamine oxidase and DAquinone (DAQ) formation. Results Here, we report that apoptotic mitochondrial fragmentation via Calcineurin (CaN)-DRP1 axis is a common downstream event for both these initial cues, inhibiting which can protect cells from DA toxicity comprehensively. No protective effect is observed, in terms of cell survival when only PxIxIT domain of CaN is obstructed, demonstrating the importance to block DRP1-CaN axis specifically. Further, evaluation of the impact of DA exposure on PD progression in a mice model reveal that LD mediated behavioral recovery diminishes with time, mostly because of continued DAergic cell death and dendritic spine loss at striatum. CaN inhibition, alone or in combination with LD, offer long term behavioral protection. This protective effect is mediated specifically by hindering CaN-DRP1 axis, whereas inhibiting interaction between CaN and other substrates, including proteins involved in neuro-inflammation, remained ineffective when LD is co-administered. Conclusions In this study, we conclude that DA toxicity can be circumvented by CaN inhibition and it can mitigate PD related behavioral aberrations by protecting neuronal architecture at striatum. We propose that CaN inhibitors might extend the therapeutic efficacy of LD treatment.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2045-3701Test

الوصول الحر: https://doaj.org/article/4ac7a6e4d1474e80aa375dc6217461c5Test

المؤلفون: Joy Chatterjee, Raj Kumar Koninti, Prakash Panwaria, Partha Hazra

المصدر: Chemical Physics Impact, Vol 7, Iss , Pp 100285- (2023)

مصطلحات موضوعية: Excited state double proton transfer, Silica nano-channels, Sequential pathway, Concerted pathway, Time-resolved fluorescence, Femtosecond fluorescence upconversion, Physics, QC1-999, Chemistry, QD1-999

الوصف: The modulation of kinetics and pathways in the ESIPT process of proton transfer probes holds significant potential for advancing applications in bio-imaging, drug delivery, and OLEDs. One effective approach for achieving this modulation is altering the H-bonding donating capability of the surrounding medium. To investigate this, we conducted a comprehensive study on the excited state intramolecular double proton transfer process of [2,2′-bipyridyl]-3,3′-diol (BP(OH)2) within the confined spaces of silica nanochannels, namely, MCM-41. MCM-41, known for its versatile properties, has emerged as a promising host in various fields, such as drug delivery and heterogeneous catalysis. Upon encapsulation within the MCM-41, the double proton transfer process of BP(OH)2 is significantly modulated, which is reflected in both steady-state and time-resolved photophysical experiments. We have observed an almost 100 times increment in emission intensity and a 30 nm blue-shift in the emission maxima when the probe gets encapsulated inside the silica nanopores. Most importantly, the femtosecond up-conversion profile exhibits an interesting feature. The rise component of 10 ps, which was attributed to MK→DK conversion in bulk acetonitrile (MeCN), is not observed when the probe resides inside the MCM-41, suggesting the proton transfer is concerted rather than sequential, like in the case of bulk MeCN. This anomalous proton transfer mechanism inside the nanochannel was attributed to the weak H-bonding donating ability of the silanol groups, which could not stabilize the MK form, and thus favoured the concerted pathway over sequential. Moreover, DFT calculations corroborate the concerted pathway observed in the MCM-41 with the gas-phase calculations and the sequential mechanism observed in bulk MeCN with the solution-phase calculations.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2667022423001251Test; https://doaj.org/toc/2667-0224Test

الوصول الحر: https://doaj.org/article/8c5e1cb8c58a486591f2a2e0086402b7Test

المؤلفون: Rupsha Mondal, Chayan Banerjee, Sumangal Nandy, Moumita Roy, Joy Chakraborty

المصدر: IBRO Neuroscience Reports, Vol 15, Iss , Pp S452- (2023)

مصطلحات موضوعية: Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2667242123009375Test; https://doaj.org/toc/2667-2421Test

الوصول الحر: https://doaj.org/article/0f8c792664fb41d6ab74a01a16acbf90Test

المؤلفون: Chayan Banerjee, Moumita Roy, Rupsha Mondal, Joy Chakraborty

المصدر: Frontiers in Cell and Developmental Biology, Vol 11 (2023)

مصطلحات موضوعية: USP14, Prohibitin2, mitophagy, neurodegeneration, Parkinson’s disease, substantia nigra, Biology (General), QH301-705.5

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fcell.2023.1244890/fullTest; https://doaj.org/toc/2296-634XTest

الوصول الحر: https://doaj.org/article/fe02a5d594dd4139aa80bb450e80e728Test

المؤلفون: Md Saddam Hossain, Sadia Jahan, Sad Al Rezwan Rahman, Mashiur Rahman, Diponkor Kumar, Susmita Paul, Joy Chandra Rajbangshi

المصدر: Heliyon, Vol 9, Iss 3, Pp e14259- (2023)

مصطلحات موضوعية: Immediate, Design expert, Combination, Anti-diabetic, Streptozotocin, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: Background: The combination of empagliflozin and sitagliptin to treat type-2 diabetes might be more economical and patient compliance with an additive improvement in glycemic control due to complementary modes of action. Aim of the study: To design, formulate and optimize an immediate tablet dosage form containing empagliflozin and sitagliptin utilizing statistically reliable study design followed by in-vitro and in-vivo testing. Method: ology: To determine the effects of copovidone (X1) and croscarmellose sodium (X2) amounts on the dependent variables of disintegration time and percent drug release, the formulation was developed using Design Expert Software v.13's direct compression method-based central composite design optimization study. The formulations' assay, dissolution, friability, hardness, weight variation, disintegration, and anti-diabetic effects were evaluated in comparison to the standard drug. The analysis included the use of high performance liquid chromatography (HPLC) assay methods. Mice were employed to investigate the efficacy of an anti-diabetic drug after they were administered a high-fat diet and two injections of streptozotocin at a dosage of 30 mg/kg BW each. Results: Formulation of F3 out of nine had all in-vitro parameters at the most satisfactory condition. It was found that assay of the best formulation is 100.99% and 100.19% for empagliflozin and sitagliptin respectively. The disintegration time of F3 was found at 5.32 min. Percentage release of empagliflozin in 30 min was found 89.05% while sitagliptin was with 93.76%. The results showed that administration of F3 significantly reduced FBG (68.61%, p

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844023014664Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/f769e864d4b249a499d2da4471edfc24Test

المؤلفون: Chisom Emeka, Betta Edu, Janet Ekpenyong, Biniam Getachew, Joy Chabo, Umar Pella Abdurhaman, Nyambu Bernard Ntui, Ephraim Chukwu, Nsa Ekpenyong

المصدر: Public Health in Practice, Vol 4, Iss , Pp 100273- (2022)

مصطلحات موضوعية: COVID-19, Vaccine, Vaccine roll-out, Vaccination, COVID-19 vaccination, Public aspects of medicine, RA1-1270

الوصف: Since the first COVID-19 case was reported in Nigeria in February 2020, the Country’s effort to curb the surge in cases and protect people from the disease was undeniable, as does Cross River State (CRS). Using document revision, we illustrate the COVID-19 vaccine rollout in Cross River State, Nigeria. The State recorded its first COVID-19 cases on June 29, 2020. COVID-19 vaccination commenced in the State on March 11, 2021. The pandemic response was led by the COVID -19 taskforce constituted by the Government of CRS in March 2020 to ensure effective response to effective response to the pandemic.Intensified advocacy, communication and social mobilization activities, mainly community engagement, were conducted to minimize vaccine hesitancy. A chain of responsibilities was observed in vaccine management and logistics. The State carried out a successful rollout of the first phase of COVID-19 vaccination, including refugees’ vaccination and management of AEFI. This commentary aims to share the experience and lessons learned in rolling out the COVID-19 vaccine in Cross River State, Nigeria. This paper will guide policymakers in developing countries.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2666535222000490Test; https://doaj.org/toc/2666-5352Test

الوصول الحر: https://doaj.org/article/a5a5796d905143248144e03e91114b38Test

المؤلفون: Gabriel M Garcia, Joy Chavez Mapaye, Travis Hedwig, Jessica Petalio, Suzanna Rosie Aquino, Pauline Lasquete, Claudine Tungul

المصدر: International Journal of Circumpolar Health, Vol 81, Iss 1 (2022)

مصطلحات موضوعية: Filipino American, colorectal cancer screening promotion, Alaska, Arctic medicine. Tropical medicine, RC955-962

الوصف: This study assessed the feasibility of implementing Project Buhay (PB), the first colorectal cancer (CRC) screening promotion programme for Filipinos in Alaska and developed through university-community partnership. PB involved piloting two interventions: a group health education intervention and (GHEI) a video-based intervention (VBI) showing a mini-documentary of a Filipina from Alaska with CRC. Participants included self-identified Filipinos, aged 50 to 75 years who were not current in CRC screening. Data collected include recruitment, reach, implementation process, short-term outcomes, and implementation barriers. Results show that PB reached a total of three Alaskan communities and exposed almost 50 participants. GHEI and VBI participants were followed-up at three-month post-intervention, with 80% reporting their intention to get CRC screening within a year. The main barrier in implementing PB was its lack of funding and time, which lessened effectiveness and reduced community and participant reach. However, PB team's ability to make adjustments in implementation and leverage existing university and community assets led to the successful implementation of theinterventions. At the project’s conclusion, there were positive implications for both the Filipino community in Alaska and project team, affirming the importance of university-community partnership.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2242-3982Test

الوصول الحر: https://doaj.org/article/8e1c5dd4b857434cbe3d926a81cf63d5Test

المؤلفون: Joshua DeVos, Kimberly McCarthy, Victor Sewe, Grace Akinyi, Muthoni Junghae, Valarie Opollo, Janin Nouhin, Robert Shafer, Clement Zeh, Artur Ramos, Heather Alexander, Joy Chang

المصدر: Microbiology Spectrum, Vol 10, Iss 3 (2022)

مصطلحات موضوعية: drug resistance, human immunodeficiency virus, integrase, Microbiology, QR1-502

الوصف: ABSTRACT As dolutegravir (DTG)-containing HIV regimens are scaled up globally, monitoring for HIV drug resistance (HIVDR) will become increasingly important. We designed a partially multiplexed HIVDR assay using Sanger sequencing technology to monitor HIVDR mutations in the protease, reverse-transcriptase (PRRT), and integrase (INT). A total of 213 clinical and analytical plasma and dried blood spot (DBS) samples were used in the evaluation. The assay detected a wide range of known HIV-1 subtypes and circulating recombinant forms (CRFs) of group M from 139 samples. INT accuracy showed that the average nucleotide (nt) sequence concordance was 99.8% for 75 plasma samples and 99.5% for 11 DBS samples compared with the reference sequences. The PRRT accuracy also demonstrated the average nucleotide sequence concordance was 99.5% for 57 plasma samples and 99.2% for 33 DBS samples. The major PRRT and INT DR mutations of all samples tested were concordant with those of the reference sequences using the Stanford HIV database (db). Amplification sensitivity of samples with viral load (VL) >5000 copies/mL showed plasma exceeded 95% of positivity, and DBS exceeded 90% for PRRT and INT. Samples with VL (1000 to 5000 copies/mL) showed plasma exceeded 90%, and DBS reached 88% positivity for PRRT and INT. Assay precision and reproducibility showed >99% nucleotide sequence concordance in each set of replicates for PRRT and INT. In conclusion, this HIVDR assay met WHO HIVDR assay performance criteria for surveillance, worked for plasma and DBS, used minimal sample volume, was sensitive, and was a potentially cost-effective tool to monitor HIVDR mutations in PRRT and INT. IMPORTANCE This HIVDR genotyping assay works for both plasma and DBS samples, requires low sample input, and is sensitive. This assay has the potential to be a user-friendly and cost-effective HIVDR assay because of its partially multiplexed design. Application of this genotyping assay will help HIVDR monitoring in HIV high-burdened countries using a DGT-based HIV drug regimen recommended by the U.S. President's Emergency Plan for AIDS Relief and the WHO.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2165-0497Test

الوصول الحر: https://doaj.org/article/08a0d9d70f5e44f38b42f19ef2619bd5Test