المؤلفون: Johnson, Darryl N., Ruan, Zheng, Petley, Emma V., Devi, Sapna, Holz, Lauren E., Uldrich, Adam P., Mak, Jeffrey Y. W., Hor, Jyh Liang, Mueller, Scott N., McCluskey, James, Fairlie, David P., Darcy, Phillip K., Beavis, Paul A., Heath, William R., Godfrey, Dale I.

المساهمون: Australian Research Council Center of Excellence in Advanced Molecular Imaging, National Health and Medical Research Council

المصدر: Scientific Reports ; volume 12, issue 1 ; ISSN 2045-2322

مصطلحات موضوعية: Multidisciplinary

الوصف: Natural Killer T (NKT) cells and Mucosal-Associated Invariant T (MAIT) cells are innate-like T cells that express semi-invariant αβ T cell receptors (TCRs) through which they recognise CD1d and MR1 molecules, respectively, in complex with specific ligands. These cells play important roles in health and disease in many organs, but their precise intra-organ location is not well established. Here, using CD1d and MR1 tetramer staining techniques, we describe the precise location of NKT and MAIT cells in lymphoid and peripheral organs. Within the thymus, NKT cells were concentrated in the medullary side of the corticomedullary junction. In spleen and lymph nodes, NKT cells were mainly localised within T cell zones, although following in vivo activation with the potent NKT-cell ligand α-GalCer, they expanded throughout the spleen. MAIT cells were clearly detectable in Vα19 TCR transgenic mice and were rare but detectable in lymphoid tissue of non-transgenic mice. In contrast to NKT cells, MAIT cells were more closely associated with the B cell zone and red pulp of the spleen. Accordingly, we have provided an extensive analysis of the in situ localisation of NKT and MAIT cells and suggest differences between the intra-organ location of these two cell types.

الإتاحة: https://doi.org/10.1038/s41598-022-07704-4Test
https://www.nature.com/articles/s41598-022-07704-4.pdfTest
https://www.nature.com/articles/s41598-022-07704-4Test

المؤلفون: Holz, Lauren E., Prier, Julia E., Freestone, David, Steiner, Thiago M., English, Kieran, Johnson, Darryl N., Mollard, Vanessa, Cozijnsen, Anton, Davey, Gayle M, Godfrey, Dale I, Yui, Katsuyuki, Mackay, Laura K., Lahoud, Mireille H., Caminschi, Irina, McFadden, Geoffrey I, Bertolino, Patrick, Fernandez-Ruiz, Daniel, Heath, William R

المصدر: Holz , L E , Prier , J E , Freestone , D , Steiner , T M , English , K , Johnson , D N , Mollard , V , Cozijnsen , A , Davey , G M , Godfrey , D I , Yui , K , Mackay , L K , Lahoud , M H , Caminschi , I , McFadden , G I , Bertolino , P , Fernandez-Ruiz , D & Heath , W R 2018 , ' CD8 + T Cell Activation Leads to Constitutive Formation of Liver Tissue-Resident Memory T Cells ....

مصطلحات موضوعية: liver, liver immunology, malaria, niche, T cell memory, tissue-resident memory

الوصف: Liver tissue-resident memory T (Trm) cells migrate throughout the sinusoids and are capable of protecting against malaria sporozoite challenge. To gain an understanding of liver Trm cell development, we examined various conditions for their formation. Although liver Trm cells were found in naive mice, their presence was dictated by antigen specificity and required IL-15. Liver Trm cells also formed after adoptive transfer of in vitro-activated but not naive CD8 + T cells, indicating that activation was essential but that antigen presentation within the liver was not obligatory. These Trm cells patrolled the liver sinusoids with a half-life of 36 days and occupied a large niche that could be added to sequentially without effect on subsequent Trm cell cohorts. Together, our findings indicate that liver Trm cells form as a normal consequence of CD8 + T cell activation during essentially any infection but that inflammatory and antigenic signals preferentially tailor their development. Holz et al. demonstrate that tissue-resident memory T (Trm) cells routinely develop in the liver after T cell activation. Within the liver, IL-15, antigen, and inflammation aid Trm cell formation, but only IL-15 is essential. Newly formed Trm cells do not displace existing populations, demonstrating a flexible liver niche.

وصف الملف: application/pdf

الإتاحة: https://doi.org/10.1016/j.celrep.2018.08.094Test
https://research.monash.edu/en/publications/ebd4c92c-e1ec-41c7-ab9d-da085f7a7405Test
https://researchmgt.monash.edu/ws/files/255017794/252451070_oa.pdfTest
http://www.scopus.com/inward/record.url?scp=85054021238&partnerID=8YFLogxKTest

المؤلفون: Johnson, Darryl

المصدر: The English Journal, 2003 Nov 01. 93(2), 20-22.

الوصول الحر: https://www.jstor.org/stable/3650488Test

المؤلفون: Johnson, Darryl

المصدر: The English Journal, 2002 May 01. 91(5), 16-18.

الوصول الحر: https://www.jstor.org/stable/821389Test

المؤلفون: Shrivastava, Aakash, Kumar, Anil, Thomas, Jerry D, Laserson, Kayla F, Bhushan, Gyan, Carter, Melissa D, Chhabra, Mala, Mittal, Veena, Khare, Shashi, Sejvar, James J, Dwivedi, Mayank, Isenberg, Samantha L, Johnson, Rudolph, Pirkle, James L, Sharer, Jon D, Hall, Patricia L, Yadav, Rajesh, Velayudhan, Anoop, Papanna, Mohan, Singh, Pankaj, Somashekar, D, Pradhan, Arghya, Goel, Kapil, Pandey, Rajesh, Kumar, Mohan, Kumar, Satish, Chakrabarti, Amit, Sivaperumal, P, Kumar, A Ramesh, Schier, Joshua G, Chang, Arthur, Graham, Leigh Ann, Mathews, Thomas P, Johnson, Darryl, Valentin, Liza, Caldwell, Kathleen L, Jarrett, Jeffery M, Harden, Leslie A, Takeoka, Gary R, Tong, Suxiang, Queen, Krista, Paden, Clinton, Whitney, Anne, Haberling, Dana L, Singh, Ram, Singh, Ravi Shankar, Earhart, Kenneth C, Dhariwal, A C, Chauhan, L S, Venkatesh, S

المساهمون: CDC Research Project Cooperative Agreement Grant numbers

المصدر: The Lancet Global Health ; volume 5, issue 4, page e458-e466 ; ISSN 2214-109X

مصطلحات موضوعية: General Medicine

الإتاحة: https://doi.org/10.1016/s2214-109xTest(17)30035-9
https://api.elsevier.com/content/article/PII:S2214109X17300359?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2214109X17300359?httpAccept=text/plainTest

المؤلفون: Isenberg, Samantha L., Carter, Melissa D., Crow, Brian S., Graham, Leigh Ann, Johnson, Darryl, Beninato, Nick, Steele, Kandace, Thomas, Jerry D., Johnson, Rudolph C.

المساهمون: Centers for Disease Control and Prevention, Oak Ridge Institute for Science and Education

المصدر: Journal of Analytical Toxicology ; volume 40, issue 4, page 248-254 ; ISSN 0146-4760 1945-2403

مصطلحات موضوعية: Chemical Health and Safety, Health, Toxicology and Mutagenesis, Toxicology, Environmental Chemistry, Analytical Chemistry

الإتاحة: https://doi.org/10.1093/jat/bkw015Test
http://academic.oup.com/jat/article-pdf/40/4/248/6777109/bkw015.pdfTest

المؤلفون: Kuhns, Lisa G., Benoit, Stéphane L., Bayyareddy, Krishnareddy, Johnson, Darryl, Orlando, Ron, Evans, Alexandra L., Waldrop, Grover L., Maier, Robert J.

المساهمون: Christie, P. J., UGA Research Foundation

المصدر: Journal of Bacteriology ; volume 198, issue 9, page 1423-1428 ; ISSN 0021-9193 1098-5530

الوصف: A molecular hydrogen (H 2 )-stimulated, chemolithoautotrophic growth mode for the gastric pathogen Helicobacter pylori is reported. In a culture medium containing peptides and amino acids, H 2 -supplied cells consistently achieved 40 to 60% greater growth yield in 16 h and accumulated 3-fold more carbon from [ 14 C]bicarbonate (on a per cell basis) in a 10-h period than cells without H 2 . Global proteomic comparisons of cells supplied with different atmospheric conditions revealed that addition of H 2 led to increased amounts of hydrogenase and the biotin carboxylase subunit of acetyl coenzyme A (acetyl-CoA) carboxylase (ACC), as well as other proteins involved in various cellular functions, including amino acid metabolism, heme synthesis, or protein degradation. In agreement with this result, H 2 -supplied cells contained 3-fold more ACC activity than cells without H 2 . Other possible carbon dioxide (CO 2 ) fixation enzymes were not up-expressed under the H 2 -containing atmosphere. As the gastric mucus is limited in carbon and energy sources and the bacterium lacks mucinase, this new growth mode may contribute to the persistence of the pathogen in vivo . This is the first time that chemolithoautotrophic growth is described for a pathogen. IMPORTANCE Many pathogens must survive within host areas that are poorly supplied with carbon and energy sources, and the gastric pathogen Helicobacter pylori resides almost exclusively in the nutritionally stringent mucus barrier of its host. Although this bacterium is already known to be highly adaptable to gastric niches, a new aspect of its metabolic flexibility, whereby molecular hydrogen use (energy) is coupled to carbon dioxide fixation (carbon acquisition) via a described carbon fixation enzyme, is shown here. This growth mode, which supplements heterotrophy, is termed chemolithoautotrophy and has not been previously reported for a pathogen.

الإتاحة: https://doi.org/10.1128/jb.00041-16Test

المؤلفون: Huang, Guozhong, Ulrich, Paul N., Storey, Melissa, Johnson, Darryl, Tischer, Julie, Tovar, Javier A, Moreno, Silvia N. J., Orlando, Ron, Docampo, Robert

المصدر: Biology Faculty Publications

مصطلحات موضوعية: Biology

الوصف: Acidocalcisomes are acidic organelles present in a diverse range of organisms from bacteria to human cells. In this study acidocalcisomes were purified from the model organism Trypanosoma brucei, and their protein composition was determined by mass spectrometry. The results, along with those that we previously reported, show that acidocalcisomes are rich in pumps and transporters, involved in phosphate and cation homeostasis, and calcium signaling. We validated the acidocalcisome localization of seven new, putative, acidocalcisome proteins (phosphate transporter, vacuolar H+-ATPase subunits a and d, vacuolar iron transporter, zinc transporter, polyamine transporter, and acid phosphatase), confirmed the presence of six previously characterized acidocalcisome proteins, and validated the localization of five novel proteins to different subcellular compartments by expressing them fused to epitope tags in their endogenous loci or by immunofluorescence microscopy with specific antibodies. Knockdown of several newly identified acidocalcisome proteins by RNA interference (RNAi) revealed that they are essential for the survival of the parasites. These results provide a comprehensive insight into the unique composition of acidocalcisomes of T. brucei, an important eukaryotic pathogen, and direct evidence that acidocalcisomes are especially adapted for the accumulation of polyphosphate.

وصف الملف: application/pdf

العلاقة: https://scholarworks.gsu.edu/biology_facpub/47Test; https://scholarworks.gsu.edu/context/biology_facpub/article/1048/viewcontent/PLoS_Pathog__10__12___e1004555_biol.PDFTest; https://scholarworks.gsu.edu/context/biology_facpub/article/1048/filename/0/type/additional/viewcontent/1268694.zipTest

الإتاحة: https://scholarworks.gsu.edu/biology_facpub/47Test
https://scholarworks.gsu.edu/context/biology_facpub/article/1048/viewcontent/PLoS_Pathog__10__12___e1004555_biol.PDFTest
https://scholarworks.gsu.edu/context/biology_facpub/article/1048/filename/0/type/additional/viewcontent/1268694.zipTest

المؤلفون: Hernández-Rodríguez, Yainitza, Masuo, Shunsuke, Johnson, Darryl, Orlando, Ron, Smith, Amy, Couto-Rodriguez, Mara, Momany, Michelle

المساهمون: Goldman, Gustavo Henrique

المصدر: PLoS ONE ; volume 9, issue 3, page e92819 ; ISSN 1932-6203

الإتاحة: https://doi.org/10.1371/journal.pone.0092819Test

المؤلفون: Bordeaux, J. Michael, Lorenz, W. Walter, Johnson, Darryl, Badgett, Majors J., Glushka, John, Orlando, Ronald, Dean, Jeffrey F. D.

المصدر: Journal of Economic Entomology ; volume 107, issue 5, page 1931-1945 ; ISSN 0022-0493 0022-0493

مصطلحات موضوعية: Insect Science, Ecology, General Medicine

الإتاحة: https://doi.org/10.1603/ec14151Test
http://academic.oup.com/jee/article-pdf/107/5/1931/19248899/jee107-1931.pdfTest