المؤلفون: Wei Chen, Wang Peng, Ronghua Wang, Shuya Bai, Mengdie Cao, Si Xiong, Yanling Li, Yilei Yang, Jingwen Liang, Luyao Liu, Hamza O. Yazdani, Yuchong Zhao, Bin Cheng

المصدر: Cell Death and Disease, Vol 15, Iss 1, Pp 1-17 (2024)

مصطلحات موضوعية: Cytology, QH573-671

الوصف: Abstract Early metastasis is the primary factor in the very poor prognosis of pancreatic ductal adenocarcinoma (PDAC), with liver metastasis being the most common form of distant metastasis in PDAC. To investigate the mechanism of PDAC liver metastasis, we found that PDAC cells can promote the formation of pre-metastatic niches (PMNs) through exosomes to facilitate liver metastasis in the early stage. In our study, hepatic stellate cells (HSCs) were treated with PDAC-derived exosomes (PDAC-exo), and the activation of HSCs was detected. A novel transfer RNA-derived fragment, the tRF-GluCTC-0005 was obtained by small RNA sequencing from serum exosomes of PDAC patients. Bioinformatics analysis and RNA pull-down assays revealed the interaction between WDR1 and tRF-GluCTC-0005. A KPC transgenic mouse model and an AAV-mediated sh-WDR1 mouse model were used to detect the mechanism of liver metastasis in vivo. Finally, the dual luciferase reporter assay, protein mutation truncation assay, Co-IP assay, and flow cytometry assay were used to explore the molecular mechanism in HSCs activation and PMNs formation. We found that the tRF-GluCTC-0005 in exosomes binds to the 3’ untranslated region of the mRNA of the WDRl in HSCs and increases mRNA stability. The N-terminals of WDR1 bind to the YAP protein directly, inhibit YAP phosphorylation, and promote the expression of YAP transcription factors. The tRF-GluCTC-0005 in PDAC-exo significantly recruits myeloid-derived suppressor cells (MDSCs) in the liver, creating a PMNs immunosuppressive microenvironment and further advancing liver metastasis from PDAC. Our results suggest that the key of PDAC liver metastasis is the activation of HSCs through upregulation of WDR1 by tRF-GluCTC-0005 in exosomes, which mediates the infiltration of MDSCs to form PMNs.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2041-4889Test

الوصول الحر: https://doaj.org/article/26f926f59c53428984d25246a58aea95Test

المؤلفون: Kaitao Zhai, Yongchun Hua, Jingwen Liang, Jing Li, Zirui Wang, Lei Liu, Minglong Gao, Rula Sa, Mingmin Zhao

المصدر: Frontiers in Microbiology, Vol 14 (2023)

مصطلحات موضوعية: Greater Khingan Mountains, birch secondary forest, bacterial diversity, fungi diversity, forest ecological cycle, Microbiology, QR1-502

الوصف: IntroductionSoil microorganisms are an important component of soil ecosystems with an indispensable role in forest ecosystems. We analyzed the soil microbial diversity in birch secondary forest formed by natural restoration or artificial reconstruction after interference by burning, clear cutting, and gradient cutting, and the Betula platyphylla Suk undisturbed forest in the Greater Khingan Mountains in China.MethodsIllumina high-throughput sequencing technology was used to analyze the characteristics of the soil microbial community during the restoration process of birch secondary forest caused by the different types of interference. The relationships between bacteria and fungi were analyzed. The gene functions of the soil bacterial community and the ecological functions of soil fungi were predicted using PICRUSt and FunGuild, respectively.ResultsAt the phylum level, the species and quantity of bacteria were more abundant than that of fungi. At the genus level, no obvious differences in the abundance of bacteria were observed; there were obvious differences in the abundance of fungi. Among the eight sample plots, the artificial larch forest belt had the highest bacterial and fungal alpha diversity, which was slightly higher than undisturbed forest, while the other sample plots were significantly lower. Gradual cutting pure birch forest bacteria and fungi had the highest beta diversity, and artificial larch forest belt bacteria and heavy burn sample plot fungi had the lowest beta diversity. Samples from the cutting and burning sample plots were significantly different from the undisturbed forest at the phylum level of Acidobacteriae, Acidimicrobiia, Mortierellomycetes and Sordariomycetes. We found statistical differences in biomarkers between bacterial and fungal communities in undisturbed forest and artificial larch forest belt and burn sample plots. PICRUSt prediction and FunGuild prediction showed that soil bacterial and fungal communities were rich in gene and ecological functions, respectively. In the microbial network, the stability or anti-interference performance of the fungal community was higher than that of bacteria.ConclusionOur data reveal the characteristics of the soil microbial community during the restoration process of Betula platyphylla Suk secondary forest under different types of disturbance, which is of great significance for understanding the role of soil microorganisms in the forest ecological cycle.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fmicb.2023.1267746/fullTest; https://doaj.org/toc/1664-302XTest

الوصول الحر: https://doaj.org/article/4fd0ffaed36c4c70bcb04ddc615102d4Test

المؤلفون: Panfeng Yao, Chunli Zhang, Dan Zhang, Tianyuan Qin, Xiaofei Xie, Yuhui Liu, Zhen Liu, Jiangping Bai, Zhenzhen Bi, Junmei Cui, Jingwen Liang, Chao Sun

المصدر: Plants, Vol 12, Iss 22, p 3809 (2023)

مصطلحات موضوعية: potato, AAO family, drought stress, expression patterns, Botany, QK1-989

الوصف: Abscisic acid (ABA) is an important stress hormone that affects plants’ tolerance to stress. Changes in the content of abscisic can have an impact on plant responses to abiotic stress. The abscisic acid aldehyde oxidase (AAO) plays a crucial role in the final step in the synthesis of abscisic acid; therefore, understanding the function of the AAO gene family is of great significance for insight into plants’ response to abiotic stresses. In this study, Solanum tuberosum AAO (StAAO) members were exhaustively explored using genome databases, and nine StAAOs were identified. Chromosomal location analysis indicated that StAAO genes mapped to 4 of the 14 potato chromosomes. Further analyses of gene structure and motif composition showed that members of the specific StAAO subfamily showed relatively conserved characteristics. Phylogenetic relationship analysis indicated that StAAOs proteins were divided into three major clades. Promoter analysis showed that most StAAO promoters contained cis-elements related to abiotic stress response and plant hormones. The results of tissue-specific expression analysis indicated that StAAO4 was predominantly expressed in the roots. Analysis of transcriptome data revealed that StAAO2/4/6 genes responded significantly to drought treatments. Moreover, further qRT-PCR analysis results indicated that StAAO2/4/6 not only significantly responded to drought stress but also to various phytohormone (ABA, SA, and MeJA) and abiotic stresses (salt and low temperature), albeit with different expression patterns. In summary, our study provides comprehensive insights into the sequence characteristics, structural properties, evolutionary relationships, and expression patterns of the StAAO gene family. These findings lay the foundation for a deeper understanding of the StAAO gene family and offer a potential genetic resource for breeding drought-resistant potato varieties.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2223-7747/12/22/3809Test; https://doaj.org/toc/2223-7747Test

الوصول الحر: https://doaj.org/article/9a588a6db959417cba514dff9d11db04Test

المؤلفون: Qi Zhang, Zheng Liang, Xiaotao Guan, Jingwen Liang, Pan Gao

المصدر: Frontiers in Environmental Science, Vol 10 (2022)

مصطلحات موضوعية: railway wastewater, micro-electrolytic, iron-carbon microbial galvanic cell, combined wastewater treatment, MBR, carbon emissions, Environmental sciences, GE1-350

الوصف: The application of iron–carbon microbial cell activated sludge (ICMC-AS) was carried out in a membrane bioreactor (MBR) processor to treat wastewater from an integrated railway station. Results showed that the chemical oxygen demand (COD), total nitrogen (TN), and total phosphorus (TP) removal efficiencies of the original MBR processor increased from 80%, 30%, and 10% to 92%, 93.5%, and 92%, respectively. Further research showed that the combined sewage treatment system also had a strong impact resistance ability. The combined sewage treatment system ran stably when the COD, TN, and TP concentrations changed greatly. The in-depth analysis of the reaction process and reaction rate of the combined sewage treatment system revealed that the combined system is dominated by COD removal with high nitrogen removal efficiency. The removal rate constants of various pollutants were in the order: KCOD (0.647 ± 0.017) > KNO3−−N (0.416 ± 0.044) > KNH4+−N (0.275 ± 0.014) > KTN (0.258 ± 0.083). Calculations of the energy saving and carbon emission reduction of the combined system showed that the system’s annual carbon emission reduction could reach more than 388,203.55 kg CO2e, which remarkably improves the carbon emission reduction effect and obtains a good green effect. The results indicate that adding ICMC-AS to the MBR processor for combined wastewater treatment can substantially improve the efficiency of wastewater treatment and obtain better energy-saving and emission-reducing effects. This combined application provides an effective way for the transformation and upgrading of small- and medium-scale water treatment systems.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fenvs.2022.1085386/fullTest; https://doaj.org/toc/2296-665XTest

الوصول الحر: https://doaj.org/article/f7ecfd68d734438ba99320bd44a54e9fTest

المؤلفون: Jingwen Liang, Zhuoheng Yu, Zhangxing Wang, Jianxia Chen, Yihuan Liu, Zhaoqing Yin, Ruihuan Xu

المصدر: Frontiers in Pediatrics, Vol 10 (2022)

مصطلحات موضوعية: TMPRSS3, homozygous, non-syndromic hearing loss, whole-exome sequencing, mutation, Pediatrics, RJ1-570

الوصف: BackgroundDeafness is the most common sensory defect in humans worldwide. Approximately 50% of cases are attributed to genetic factors, and about 70% are non-syndromic hearing loss (NSHL).ObjectivesTo identify clinically relevant gene variants associated with NSHL in a Chinese family using trio-based whole-exome sequencing (WES).Materials and methodsWES was performed on the 18-month-old female proband, and her parents. Gene variants specific to the family were identified by bioinformatics analysis and evaluated for their relevance to NSHL. We verified the novel variant in this family by the next-generation sequencing.In order to elucidate the frameshift mutation of TMPRSS3 in a Chinese family, we used the Mass spectrometry to detect the gene from 1,010 healthy subjects.ResultsWe identified a novel homozygous deletion (c.51delA) in exon 2 of the type II transmembrane serine protease 3 gene TMPRSS3, which resulted in a frameshift mutation just before the protein transmembrane domain (p.Q17fs). The deletion was present in the proband and her father, but not in her mother and the healthy controls. We also found mutations with potential relevance to hearing loss in DCAF17, which encodes a protein of unknown function (c. T555A: p.H185Q), and ZNF276, which encodes zinc finger protein 276 (c.1350–2A > G).Conclusions and significanceWe shown a novel frameshift mutation in TMPRSS3 associated with autosomal recessive NSHL in a Han Chinese family.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fped.2022.1032659/fullTest; https://doaj.org/toc/2296-2360Test

الوصول الحر: https://doaj.org/article/42ef3fe051d2499282bc688a54d68b0dTest

المؤلفون: Yuchi Zhang, Dongwei Han, Xiaoyan Yu, Xinyu Shao, Chuju Zong, Manyu Zhang, Junzhi Wang, Jingwen Liang, Pengling Ge

المصدر: Frontiers in Genetics, Vol 13 (2022)

مصطلحات موضوعية: premature ovarian failure (POF), 4-vinylcyclohexene diepoxide (VCD), miRNA-190a-5p, PHLPP1, AKT, LHR, Genetics, QH426-470

الوصف: We previously screened 6 differentially expressed miRNAs in ovarian tissues of 4-vinylcyclohexene diepoxide (VCD)-treated premature ovarian failure (POF) model in SD rats, including miRNA-190a-5p, miRNA-98-5p, miRNA-29a-3p, miRNA-144-5p, miRNA-27b-3p, miRNA-151-5p. In this study, to investigate the mechanisms causing the onset of POF, we first identified miRNAs with earlier differential expression at consecutive time points in the VCD-treated rat POF model and explored the mechanisms by which the target miRNAs promote POF. The SD rats were injected with VCD for 15 days to induce POF. Additionally, we collected rat blood and ovaries at the same time every day for 15 consecutive days, and luteinizing hormone (LH), follicle-stimulating hormone (FSH), Anti-Mullerian hormone (AMH), and estradiol (E2) serum levels were detected by ELISA. Six miRNAs expression were measured in rat ovaries by qRT-PCR. Dual-luciferase reporter gene assays were employed to predict and verify the target gene (PHLPP1) of target miRNAs (miRNA-190a-5p). Western blot was examined to detect the expression levels of PHLPP1, AKT, p-AKT, FOXO3a, p-FOXO3a, and LHR proteins on the target gene PHLPP1 and its participation in the primordial follicular hyperactivation-related pathways (AKT-FOXO3a and AKT-LH/LHR). During the VCD modeling POF rat ovaries, miRNA-190a-5p was the first to show significant differential expression, i.e., 6th of VCD treating, and PHLPP1 was verified to be a direct downstream target of it. Starting from the 6th of VCD treatment, the more significant the up-regulation trend of miRNA-190a-5p expression, the more obvious the down-regulation trend of PHLPP1 and LHR mRNA and protein expression, accompanied by the more severe phosphorylation of AKT and FOXO3a proteins, thus continuously over-activating the rat primordial follicle to promote the development of POF. In conclusion, miRNA-190a-5p may become a potential biomarker for early screening of POF, and it can continuously activate primordial follicles in rats by targeting the expression of PHLPP1 and key proteins in the AKT-FOXO3a and AKT-LH/LHR pathways.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fgene.2022.1034832/fullTest; https://doaj.org/toc/1664-8021Test

الوصول الحر: https://doaj.org/article/6272bcc9c2d243efa2daa87eeb35951cTest

المؤلفون: Zhuodi Yao, Xin Jin, Jingwen Liang, Rui Wang, Pengkang Jin, Guangping Li, Liushuan Yao, Zhe Wang

المصدر: Water Reuse, Vol 11, Iss 2, Pp 236-247 (2021)

مصطلحات موضوعية: advanced treatment, dissolved ozone flotation, fracturing wastewater, reconstruction project, Environmental technology. Sanitary engineering, TD1-1066

الوصف: To solve the problems of unstable chemical oxygen demand (COD), turbidity and suspended solid (SS) removal for the electrocatalytic process and unstable operation of the subsequent ultrafiltration membrane–reverse osmosis membrane in a centralised fracturing wastewater treatment plant in Inner Mongolia, the integrated dissolved ozone flotation (DOF) process was proposed to replace the original electrocatalytic process. Multiple processes, such as ozonation, flotation, coagulation and decolourisation, can be achieved in one integrated DOF reactor. The results showed that the removal efficiency of COD, colour, turbidity and SS in the DOF process could reach 25.4, 49.9, 95 and 96%, respectively. Meanwhile, the treatment cost was reduced by 47% (i.e., 1.77 RMB/m3 for the DOF process) compared with the electrocatalytic process. HIGHLIGHTS Multiple physio-chemical processes can be achieved in one integrated DOF reactor.; The DOF exhibited higher removal efficiency compared with the electrocatalytic process.; The DOF has stable removal efficiency during drastical influent quality variation.; The treatment cost was reduced by 47% compared with the electrocatalytic process.;

وصف الملف: electronic resource

العلاقة: http://jwrd.iwaponline.com/content/11/2/236Test; https://doaj.org/toc/2709-6092Test; https://doaj.org/toc/2709-6106Test

الوصول الحر: https://doaj.org/article/96c8a8f6cf8b4aac8861dd98f3179c21Test

المؤلفون: Kangcheng Liu, Yau Kei Chan, Xia Peng, Ruolan Yuan, Min Liao, Jingwen Liang, Xiangbo Tang, Yi Xu, Yingjun Cai, Qiangxiang Li, Hua Wang

المصدر: Frontiers in Medicine, Vol 8 (2021)

مصطلحات موضوعية: dietary supplements, meibomian gland dysfunction (MGD), dry eye, Retaron®, symptoms and signs, Medicine (General), R5-920

الوصف: Purpose: To explore the therapeutic effect of a dietary supplement on dry eye with meibomian gland dysfunction (MGD).Methods: Sixty patients with MGD-related dry eye were included in this prospective and randomized, placebo-controlled study. All the subjects were treated with eye hot compress, artificial tears, and antibiotic ointment. After that, the patients received dietary supplementary or placebo daily for 12 weeks. The dry eye signs, function of MG, and visual quality of the patients were assessed at 4, 8, and 12 weeks after the treatment.Results: Twelve weeks after the treatment, patients who received dietary supplement had a significantly better improvement of dry eye symptoms, in terms of ocular surface diseases index and tear breaking-up time (TBUT), than those who received placebo (P < 0.05). The functions of MG, in terms of meibum quality and MG exclusion and MG obstruction scores, were significantly improved in both dietary supplement and placebo groups (P < 0.05). Patients who received dietary supplement had a significantly better improvement in the MG structure, in terms of acinar diameter and acinar density, than those who received placebo (P < 0.05). The number of inflammatory cells near MG was significantly lower in the dietary supplement group when compared with the placebo group (P < 0.05). The objective visual quality was significantly improved in the dietary supplement group, but not in the placebo group (P < 0.05).Conclusion: The dietary supplement can effectively improve the symptoms and signs of MGD-related dry eye, reduce the inflammatory reaction of MG, restore the gland structure, and indirectly improve the visual quality.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fmed.2021.769132/fullTest; https://doaj.org/toc/2296-858XTest

الوصول الحر: https://doaj.org/article/898edfc2afb441a2bbb7557b48ab9a13Test

المؤلفون: Jingwen LIANG,Yingfeng LUO,Yi SUN,Meng LEI,Bing ZHANG,Songnian HU,Yaofeng ZHAO

المصدر: Frontiers of Agricultural Science and Engineering, Vol 1, Iss 3, Pp 201-213 (2014)

مصطلحات موضوعية: immunoglobulin, heavy chain, variable region, repertoire, complementarity determining region 3 of the IgH chain (CDRH3), Agriculture (General), S1-972

الوصف: In the bone marrow and spleen, the developing B cell populations undergo both negative and positive selections to shape their B cell receptor repertoire. To gain insight into the shift of the immunoglobulin heavy (IgH) chain repertoire during B cell development, we undertook large scale Ig μ chain repertoire analysis of pre-B, immature B and spleen B cell populations. We found that the majority of VH gene segments, VH families, JH and D gene segments, were observed to have significantly different usage frequencies when three B cell populations were compared, but the usage profile of the VH, D, and JH genes between different B cell populations showed high correlations. In both productive and nonproductive rearrangements, the length of CDRH3 shortened significantly on average when B cells entered the periphery. However, the CDRH3 length distribution of nonproductive rearrangements did not follow a Gaussian distribution, but decreased successively in the order 3n-2, 3n-1 and 3n, suggesting a direct correlation between mRNA stability and CDRH3 length patterns of nonproductive rearrangements. Further analysis of the individual components comprising CDRH3 of productive rearrangements indicated that the decrease in CDRH3 length was largely due to the reduction of N addition at the 5′ and 3′ junctions. Moreover, with development, the amino acid content of CDRH3 progressed toward fewer positively charged and nonpolar residues but more polar residues. All these data indicated that the expressed Ig μ chain repertoire, especially the repertoire of CDRH3, was fine-tuned when B cells passed through several checkpoints of selection during the process of maturation.

وصف الملف: electronic resource

العلاقة: http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/2095-7505-2014-3-201.pdfTest; https://doaj.org/toc/2095-7505Test

الوصول الحر: https://doaj.org/article/e09a2c966760425da5e936f682aff625Test

المؤلفون: Jiefang Bian, Xiling Wang, Jun Yun, Ruifeng Cao, Yunxin Cao, Jingwen Liang, Fucheng Ma

المصدر: Annals of Saudi Medicine, Vol 32, Iss 3, Pp 269-275 (2012)

مصطلحات موضوعية: Medicine

الوصف: BACKGROUND AND OBJECTIVES: Radiotherapy is frequently applied in the treatment of malignant gliomas, but it is unclear if radiotherapy exerts its effects via induction of apoptosis. The present study was designed to determine whether a single-fraction γ-60Co radiation can induce apoptosis. DESIGN AND SETTING: In vitro cytological controlled study performed at a military medical university from October 2006 to June 2008. METHODS: C6 cells were treated with a single fraction of γ-60Co radiation at various doses (0, 4, 16, and 64 Gy). The 3-(4,5)-dimethylthiazol-2)-2,5-diphenyl tetrazolium bromide (MTT) assay, apoptosis assays using Annexin V-fluorescein isothiocyanate/propidium iodide or Hoechst 33258 staining, and the cell cycle assay were performed, and the expression of p53 and p21 proteins was evaluated. RESULTS: The C6 cell numbers in the 16 Gy and 64 Gy groups were much lower than in the control group at 48, 96, and 144 hours after irradiation. The irradiated cells underwent apoptosis in a dose-dependent manner. Irradiation also impacted cell cycle progression, arresting cells in the G1 phase. The p53 protein expression was shown in both the nucleus and the cytoplasm of irradiated cells, whereas p53 was only expressed in the nucleus of control (untreated) cells. The p21 protein was expressed in irradiated cells but not in control cells. CONCLUSIONS: Single-fraction γ-60Co radiation inhibited C6 cell growth by inducing apoptosis and G1 arrest, which correlated with the up-regulation of the p53-p21 pathway. The extent of apoptosis and G1 arrest was positively correlated with the dose of radiation. Better understanding of apoptosis induced by radiation therapy will help design optimal dosing schedules for radiation therapy, especially in combination with chemotherapy.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/0256-4947Test; https://doaj.org/toc/0975-4466Test

الوصول الحر: https://doaj.org/article/5fd47ec9de08439f9bfb30368ad39b44Test