المؤلفون: Xiangning Zhang, Jing Huan, Longji Wu, Zhi-Gang Huang, Zhiwei He, Hongbing Yu

المصدر: South Florida Journal of Health. 3:263-271

الوصف: Since the elucidation of the double helix structure of DNA molecule and its role of coding for genetic information, rapid progress of biomedical science including the sequencing of macromolecules in the living system has been witnessed. The technique has allowed the study field to evolve from single genes to genome in species. With demand of deposit and analysis of data obtained, a new science bioinformatics has been formed by integration of computer science and biology. Bioinformatics has found wide application in research of different disciplinary in medical science. To facilitate the grasp of its basic theory and practical application by the postgraduate students at the medical school, regular reading of relevant publications has been proposed as an approach to improve the quality of teaching, and its outcome has been evaluated by comparing the scores earned from the identical test papers. The students acquired the designed intervention had higher score in average statistically with p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::64b8c38cef9445f865e42950065a4864Test
https://doi.org/10.46981/sfjhv3n3-006Test

المؤلفون: Li, Jing-Huan, Yang, Fan, Zhou, Xu-Zhi, Zong, Qiu-Gang, Artemyev, Anton V., Rankin, Robert, Shi, Quanqi, Yao, Shutao, Liu, Han, He, Jiansen, Pu, Zuyin, Xiao, Chijie, Liu, Ji, Pollock, Craig, Le, Guan, Burch, James L.

المصدر: Nature Communications ; volume 11, issue 1 ; ISSN 2041-1723

مصطلحات موضوعية: General Physics and Astronomy, General Biochemistry, Genetics and Molecular Biology, General Chemistry, Multidisciplinary

الوصف: NASA’s Magnetospheric Multi-Scale (MMS) mission is designed to explore the proton- and electron-gyroscale kinetics of plasma turbulence where the bulk of particle acceleration and heating takes place. Understanding the nature of cross-scale structures ubiquitous as magnetic cavities is important to assess the energy partition, cascade and conversion in the plasma universe. Here, we present theoretical insight into magnetic cavities by deriving a self-consistent, kinetic theory of these coherent structures. By taking advantage of the multipoint measurements from the MMS constellation, we demonstrate that our kinetic model can utilize magnetic cavity observations by one MMS spacecraft to predict measurements from a second/third spacecraft. The methodology of “observe and predict” validates the theory we have derived, and confirms that nested magnetic cavities are self-organized plasma structures supported by trapped proton and electron populations in analogous to the classical theta-pinches in laboratory plasmas.

الإتاحة: https://doi.org/10.1038/s41467-020-19442-0Test
https://www.nature.com/articles/s41467-020-19442-0.pdfTest
https://www.nature.com/articles/s41467-020-19442-0Test

المؤلفون: Shen, Yi-Fan, Huang, Jing-Huan, Wang, Kai-Yang, Zheng, Jin, Cai, Lin, Gao, Hong, Li, Xiao-Lin, Li, Jing-Feng

المساهمون: National Natural Science Foundation of China

المصدر: Cell Communication and Signaling ; volume 18, issue 1 ; ISSN 1478-811X

مصطلحات موضوعية: Cell Biology, Molecular Biology, Biochemistry

الوصف: Background Diabetic wounds are a disturbing and rapidly growing clinical problem. A novel peptide, parathyroid hormone related peptide (PTHrP-2), is assumed as multifunctional factor in angiogenesis, fibrogenesis and re-epithelization. This study aims to test PTHrP-2 efficiency and mechanism in wound healing. Methods Through repair phenomenon in vivo some problems were detected, and further research on their mechanisms was made. In vivo therapeutic effects of PTHrP-2 were determined by HE, Masson, microfil and immunohistochemical staining. In vitro direct effects of PTHrP-2 were determined by proliferation, migration, Vascular Endothelial Grown Factor and collagen I secretion of cells and Akt/ Erk1/2 pathway change. In vitro indirect effects of PTHrP-2 was study via exosomes. Exosomes from PTHrP-2 untreated and treated HUVECs and HFF-1 cells were insolated and identified. Exosomes were co-cultured with original cells, HUVECs or HFF-1 cells, and epithelial cells. Proliferation and migration and pathway change were observed. PTHrP-2-HUVEC-Exos were added into in vivo wound to testify its hub role in PTHrP-2 indirect effects in wound healing. Results In vivo, PTHrP-2 exerted multifunctional pro-angiogenesis, pro-firbogenesis and re-epithelization effects. In vitro, PTHrP-2 promoted proliferation and migration of endothelial and fibroblast cells, but had no effect on epithelial cells. Therefore, we tested PTHrP-2 indirect effects via exosomes. PTHrP-2 intensified intercellular communication between endothelial cells and fibroblasts and initiated endothelial-epithelial intercellular communication. PTHrP-2-HUVEC-Exos played a hub role in PTHrP-2 indirect effects in wound healing. Conclusion These findings of this study indicated that PTHrP-2, a multifunctional factor, could promote wound healing via synergistic multicellular stimulating and exosomal activities. Graphical abstract

الإتاحة: https://doi.org/10.1186/s12964-020-00541-wTest

المؤلفون: Li, Zhong-jie, Dai, Hui-qi, Huang, Xiao-wei, Feng, Ji, Deng, Jing-huan, Wang, Zi-xuan, Yang, Xiao-mei, Liu, Yu-jia, Wu, Yong, Chen, Pan-hong, Shi, Huan, Wang, Ji-gang, Zhou, Jing, Lu, Guo-dong

المصدر: Acta Pharmacologica Sinica ; volume 42, issue 2, page 301-310 ; ISSN 1671-4083 1745-7254

مصطلحات موضوعية: Pharmacology (medical), Pharmacology, General Medicine

الإتاحة: https://doi.org/10.1038/s41401-020-0478-3Test
http://www.nature.com/articles/s41401-020-0478-3.pdfTest
http://www.nature.com/articles/s41401-020-0478-3Test

المؤلفون: Jing, Huan, Zhong, Deyu, Zhang, Hongwu, Shi, Xufang, Wang, Yanjun

المصدر: Journal of Geographical Sciences ; volume 30, issue 6, page 1021-1040 ; ISSN 1009-637X 1861-9568

مصطلحات موضوعية: Earth and Planetary Sciences (miscellaneous)

الإتاحة: https://doi.org/10.1007/s11442-020-1768-xTest

المؤلفون: Shen, Yi-Fan, Huang, Jing-Huan, Wang, Kai-Yang, Zheng, Jin, Cai, Lin, Gao, Hong, Li, Xiao-Lin, Li, Jing-Feng

المصدر: Cell Communication and Signaling ; volume 18, issue 1 ; ISSN 1478-811X

مصطلحات موضوعية: Cell Biology, Molecular Biology, Biochemistry

الوصف: An amendment to this paper has been published and can be accessed via the original article.

الإتاحة: https://doi.org/10.1186/s12964-020-00593-yTest

المؤلفون: Shuai, Yi, Liao, Li, Su, Xiaoxia, Sha, Nanxi, Li, Xiaobo, Wu, Yutao, Jing, Huan, Kuang, Huijuan, Deng, Zhihong, Li, Yongqi, Jin, Yan

المساهمون: national natural science foundation of china, national key research and development program of china stem cell and translational research, natural science foundation of jiangsu province, fundamental research funds for the central universities, Key Project of Sichuan province

المصدر: Therapeutic Advances in Musculoskeletal Disease ; volume 12, page 1759720X2095333 ; ISSN 1759-720X 1759-7218

مصطلحات موضوعية: Orthopedics and Sports Medicine, Rheumatology

الوصف: Aims: Osteoporosis is underdiagnosed because of the lack of a convenient diagnostic method. Circulating microRNAs (miRNAs) emerge as novel biomarkers for disease diagnosis. Here, we conducted a case-control study that included a total of 448 serum samples collected from 182 healthy participants, 132 osteopenia participants, and 134 osteoporosis patients. Methods: Circulating miRNAs dysregulated during osteoporosis were screened and analyzed in three randomly determined sub-cohorts: the discovery cohort identified 22 candidate miRNAs; the training cohort tested the candidate miRNAs and constructed Index 1, comprising five miRNAs by logistic regression, and Index 2, comprising four miRNAs, was developed by linear combination. Results: Both indices were tested in the validation cohort and showed statistically significant results in distinguishing osteoporosis patients from healthy and osteopenic patients. Moreover, Index 1 also showed improved performance over traditional bone turnover biomarkers type I pro-collagen (tPINP) and type I collagen (β-CTx). Conclusion: In conclusion, circulating miRNAs are potential biomarkers for osteoporosis. The diagnostic panel of circulating miRNAs could be a complementary method for dual-energy X-ray absorptiometry (DXA) in mass screening and routine examination to enhance the osteoporosis detection rate.

الإتاحة: https://doi.org/10.1177/1759720x20953331Test

المؤلفون: Jing-Huan Luo, Mengxiong Wu, Jianyong Liu, Guangren Qian, Zhiguo Yuan, Jianhua Guo

المصدر: Environment International, Vol 130, Iss , Pp - (2019)

مصطلحات موضوعية: Environmental sciences, GE1-350

الوصف: It has been reported that microbial reduction of sulfate, nitrite/nitrate and iron/manganese could be coupled with anaerobic oxidation of methane (AOM), which plays a significant role in controlling methane emission from anoxic niches. However, little is known about microbial chromate (Cr(VI)) reduction coupling with AOM. In this study, a microbial consortium was enriched via switching nitrate dosing to chromate feeding as the sole electron acceptor under anaerobic condition in a membrane biofilm reactor (MBfR), in which methane was continuously provided as the electron donor through bubble-less hollow fiber membranes. According to long-term reactor operation and chromium speciation analysis, soluble chromate could be reduced into Cr(III) compounds by using methane as electron donor. Fluorescence in situ hybridization and high-throughput 16S rRNA gene amplicon profiling further indicated that after feeding chromate Candidatus ‘Methanoperedens’ (a known nitrate-dependent anaerobic methane oxidation archaeon) became sole anaerobic methanotroph in the biofilm, potentially responsible for the chromate bio-reduction driven by methane. Two potential pathways of the microbial AOM-coupled chromate reduction were proposed: (i) Candidatus ‘Methanoperedens’ independently utilizes chromate as electron acceptor to form Cr(III) compounds, or (ii) Candidatus ‘Methanoperedens’ oxidizes methane to generate intermediates or electrons, which will be utilized to reduce chromate to Cr(III) compounds by unknown chromate reducers synergistically. Our findings suggest a possible link between the biogeochemical chromium and methane cycles. Keywords: Microbial chromate reduction, Anaerobic oxidation of methane, Cr(III) compounds, Candidatus ‘Methanoperedens’, Membrane biofilm reactor

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S0160412019313947Test; https://doaj.org/toc/0160-4120Test

الوصول الحر: https://doaj.org/article/fa8e2bb0c91d4b15b17b02961bd414d4Test

المؤلفون: Jing-Huan Li, Wen-Shuai Fan, Mi-Mi Wang, Yan-Hong Wang, Zheng-Gang Ren

المصدر: Journal of Translational Medicine, Vol 16, Iss 1, Pp 1-13 (2018)

مصطلحات موضوعية: Neoplasm metastasis, Mesenchymal stem cells, Animal model, Meta-analysis, Medicine

الوصف: Abstract Background It has been reported mesenchymal stem cells (MSCs) are recruited to and become integral parts of the tumor microenvironment. MSCs might have an active role in solid tumor progression, especially cancer metastasis. However, the contribution of MSCs in the process of cancer metastasis is still controversial. In this review, we performed a meta-analysis on the effects of MSCs administration on cancer metastasis based on published preclinical studies. Methods The PRISMA guidelines were used. A total of 42 publications met the inclusion criteria. Outcome data on the incidence and the number of cancer metastasis as well as study characteristics were extracted. Quality of the studies was assessed according to SYRCLE Risk of Bias tool. Random-effects meta-analysis was used to pool estimates. Results Of the 42 studies included, 32 reported that MSCs administration promoted outcome events (numbers or incidences of cancer metastasis), and 39 reported data suitable for meta-analysis. The median effect size (RR) was 2.04 for the incidence of cancer metastasis (95% CI 1.57–2.65, I2 = 21%), and the median effect size (SMD) was 1.23 for the number of cancer metastasis (95% CI 0.43–2.03, I2 = 89%). Heterogeneity was observed, with the greater impact based on study length and different ways of metastasis measurement and MSCs administration. Conclusion Our results suggested MSCs administration increased the number and the incidence of cancer metastasis in experimental cancer models. High heterogeneity and poor reported risk of bias limit the quality of these findings. Further preclinical studies with better design and adequate reporting are still needed.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12967-018-1484-9Test; https://doaj.org/toc/1479-5876Test

الوصول الحر: https://doaj.org/article/c40f61ac947f4d828cca94a1330fd6c3Test

المؤلفون: Rong-Rong Yao, Jing-Huan Li, Rui Zhang, Rong-Xin Chen, Yan-Hong Wang

المصدر: World Journal of Surgical Oncology, Vol 16, Iss 1, Pp 1-9 (2018)

مصطلحات موضوعية: Hepatocellular carcinoma, Epithelial-mesenchymal transition, Tumor-associated macrophages, Toll-like receptor 4, Surgery, RD1-811, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract Background M2-polarized macrophages are tumor-associated-macrophages (TAMs), which are important contents of tumor-infiltrating immune cells. Toll-like receptor 4 (TLR4) is a molecular biomarker of tumor aggressiveness and poor prognosis. Toll-like receptors (TLRs) have important roles in the immune system and M2-polarized macrophages. However, the effects of TLR4 on M2-polarized macrophages in hepatocellular carcinoma (HCC) are unknown. Here, TLR4 expressed on HCC cells mediates the pro-tumor effects and mechanisms of M2-polarized macrophages. Methods THP-1 cells were induced to differentiate into M2-like macrophages through treatments with IL-4, IL-13, and phorbol myristate acetate (PMA). We used the HCC cell lines SMMC-7721 and MHCC97-H cultured in conditioned medium from M2-like macrophages (M2-CM) to investigate the migration potential of HCC cells and epithelial-mesenchymal transition (EMT)-associated molecular genetics. Signaling pathways that mediated M2-CM-promoted HCC migration were detected using western blotting. Results HCC cells cultured with M2-CM displayed a fibroblast-like morphology, an increased metastatic capability, and expression of EMT markers. TLR4 expression was markedly increased in M2-CM-treated HCC cells. TLR4 overexpression promoted HCC cell migration, and a TLR4-neutralizing antibody markedly inhibited HCC EMT in cells cultured with M2-CM. Furthermore, the TLR4/(signal transducer and activator of transcription 3 (STAT3) signaling pathway contributed to the effects of M2-CM on HCC cells. Conclusions Taken together, M2-polarized macrophages facilitated the migration and EMT of HCC cells via the TLR4/STAT3 signaling pathway, suggesting that TLR4 may be a novel therapeutic target. These results improve our understanding of M2-polarized macrophages.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12957-018-1312-yTest; https://doaj.org/toc/1477-7819Test

الوصول الحر: https://doaj.org/article/8e068be4c55d40f9b4361643b65116beTest