المؤلفون: Yu Shan, Maoning Lin, Xia Sheng, Jiefang Zhang, Yaxun Sun, Guosheng Fu, Min Wang

المصدر: Frontiers in Cardiovascular Medicine, Vol 10 (2023)

مصطلحات موضوعية: left bundle branch area pacing (LBBaP), stable coronary artery disease, pacing parameters, heart failure hospitalization (HFH), safety, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: AimsStable coronary artery disease (CAD) is a prevalent comorbidity among patients requiring pacemaker implantation. This comorbidity may have an impact on the safety and prognosis of traditional right ventricular pacing (RVP). Left bundle branch area pacing (LBBaP) is a new physiological pacing modality. Our aim was to investigate the feasibility and safety of LBBaP in patients with the stable CAD.MethodsThis study included 309 patients with symptomatic bradycardia who underwent LBBaP from September 2017 to October 2021. We included 104 patients with stable CAD (CAD group) and 205 patients without CAD (non-CAD group). Additionally, 153 stable CAD patients underwent RVP, and 64 stable CAD patients underwent His-bundle pacing (HBP) were also enrolled in this study. The safety and prognosis of LBBaP was assessed by comparing pacing parameters, procedure-related complications, and clinical events.ResultsDuring a follow-up period of 17.4 ± 5.3 months, the safety assessment revealed that the overall rates of procedure-related complications were similar between the stable CAD group and the non-CAD group (7.7% vs. 3.9%). Likewise, similar rates of heart failure hospitalization (HFH) (4.8% vs. 3.4%, stable CAD vs. non-CAD) and the primary composite outcome including death due to cardiovascular disease, HFH, or the necessity for upgrading to biventricular pacing (6.7% vs. 3.9%, stable CAD vs. non-CAD), were observed. In stable CAD patients, LBBaP demonstrated lower pacing thresholds and higher R wave amplitudes when compared to HBP. Additionally, LBBaP also had significantly lower occurrences of the primary composite outcome (6.7% vs. 19.6%, P = 0.003) and HFH (4.8% vs. 13.1%, P = 0.031) than RVP in stable CAD patients, particularly among patients with the higher ventricular pacing (VP) burden (>20% and >40%).ConclusionCompared with non-CAD patients, LBBaP was found to be attainable in stable CAD patients and exhibited comparable mid-term safety and prognosis. Furthermore, in the stable CAD population, LBBaP has demonstrated more stable pacing parameters than HBP, and better prognostic outcomes compared to RVP.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fcvm.2023.1246846/fullTest; https://doaj.org/toc/2297-055XTest

الوصول الحر: https://doaj.org/article/28053a13a74944569090930da3fe98bdTest

المؤلفون: Zhimin Xue, Danyu Wu, Jiefang Zhang, Yiwen Pan, Rongsheng Kan, Jing Gao, Binquan Zhou

المصدر: Heliyon, Vol 9, Iss 11, Pp e21309- (2023)

مصطلحات موضوعية: Coronary heart disease, Procyanidin B2, Cardiomyocyte hypoxia, Oxidative stress, Apoptosis, Science (General), Q1-390, Social sciences (General), H1-99

الوصف: Background: Cardiomyocyte ischemia and hypoxia are important causes of oxidative stress damage and cardiomyocyte apoptosis in coronary heart disease (CHD). Epidemiological investigation has shown that eating more plant-based foods, such as vegetables and fruits, may significantly decrease the risk of CHD. As natural antioxidants, botanicals have fewer toxic side effects than chemical drugs and have great potential for development. Procyanidin B2 (PB2) is composed of flavan-3-ol and epicatechin and has been reported to have antioxidant and anti-inflammatory effects. However, whether PB2 exerts protective effects on hypoxic cardiomyocytes has remained unclear. This study aimed to explore the protective effect of PB2 against cardiomyocyte hypoxia and to provide new treatment strategies and ideas for myocardial ischemia and hypoxia in CHD. Methods and results: A hypoxic cardiomyocyte model was constructed, and a CCK-8 assay proved that PB2 had a protective effect on cardiomyocytes in a hypoxic environment. DCFH fluorescence staining, DHE staining, and BODIPY lipid oxidation assessment revealed that PB2 reduced the oxidative stress levels of cardiomyocytes under hypoxic conditions. TUNEL staining, Annexin V/PI fluorescence flow cytometry, and Western blot analysis of the expression of the apoptosis marker protein cleaved caspase-3 confirmed that PB2 reduced cardiomyocyte apoptosis under hypoxic conditions. JC-1 staining indicated that PB2 reduced the mitochondrial membrane potential of cardiomyocytes under hypoxia. In addition, transcriptomic analysis proved that the expression of 158 genes in cardiomyocytes was significantly changed after PB2 was added during hypoxia, of which 53 genes were upregulated and 105 genes were downregulated. GO enrichment analysis demonstrated that the activity of cytokines, extracellular matrix proteins and other molecules was changed significantly in the biological process category. KEGG enrichment analysis showed that the IL-17 signaling pathway and JAK-STAT signaling pathway underwent significant changes. We also performed metabolomic analysis and found that the levels of 51 metabolites were significantly changed after the addition of PB2 to cardiomyocytes during hypoxia. Among them, 39 metabolites exhibited increased levels, while 12 metabolites exhibited decreased levels. KEGG enrichment analysis showed that cysteine and methionine metabolism, arginine and proline metabolism and other metabolic pathways underwent remarkable changes. Conclusion: This study proves that PB2 can reduce the oxidative stress and apoptosis of cardiomyocytes during hypoxia to play a protective role. Transcriptomic and metabolomic analyses preliminarily revealed signaling pathways and metabolic pathways that are related to its protective mechanism. These findings lay a foundation for further research on the role of PB2 in the treatment of CHD and provide new ideas and new perspectives for research on PB2 in the treatment of other diseases.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2405844023085171Test; https://doaj.org/toc/2405-8440Test

الوصول الحر: https://doaj.org/article/1f46dab2a57f4b2682feed28ef45ec91Test

المؤلفون: Jiefang Zhang, Yiwen Pan, Yaxun Sun, Guosheng Fu

المصدر: ESC Heart Failure, Vol 9, Iss 2, Pp 1478-1482 (2022)

مصطلحات موضوعية: Left bundle branch area pacing, Refractory heart failure, Anatomical assessment, Histopathological evaluation, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Abstract As an emerging pacing technique, left bundle branch area pacing (LBBAP) has served as a physiological pacing modality that overcomes the limitations of His bundle pacing (HBP) or right ventricular pacing. Three patients with terminal heart failure who were waiting for heart transplantation and met the indications of pacemaker implantations received LBBAP. Symptoms were relieved and stabilized and eventually received heart transplantation. Diseased hearts from the recipients were dissected post‐transplantation, and the direct visual of pacing lead locations in the interventricular septum were evaluated, and the histopathological examination around the lead was conducted for the first time in human. As a result, we found that the locations of LBBAP leads were matched with fluoroscopic views during the procedure and Masson's staining showed extensive fibrosis occur around the lead but did not result in high thresholds.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2055-5822Test

الوصول الحر: https://doaj.org/article/4c297ad84f95439b8d0d968fd4acee2bTest

المؤلفون: Jiefang Zhang, Yaxun Sun, Zuwen Zhang, Chenyang Jiang, Guosheng Fu

المصدر: Clinical Case Reports, Vol 8, Iss 6, Pp 1030-1033 (2020)

مصطلحات موضوعية: interventricular septum, intracardiac ultrasound, left bundle branch pacing, Medicine, Medicine (General), R5-920

الوصف: Abstract Left bundle branch pacing was associated with narrower QRS in patients with pacemaker indications. LBBP guided by ICE improves the accuracy of LBBP, facilitates the lead localization, minimizes complications, and above all reduces radiation exposure time.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2050-0904Test

الوصول الحر: https://doaj.org/article/4d3322f2493d4c24ada6acfcf40e93caTest

المؤلفون: Yi Luan, Jiefang Zhang, Min Wang, Guosheng Fu, Wenbin Zhang

المصدر: Anatolian Journal of Cardiology, Vol 23, Iss 3, Pp 141-150 (2020)

مصطلحات موضوعية: advanced glycation end products, cardiac microvascular endothelial cells, diabetic cardiomyopathy, protein kinase c, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Objective: To investigate the effects of advanced glycation end products (AGEs) on the proliferation and apoptosis of cardiac microvascular endothelial cells (CMECs) in rats and their underlying signaling pathway. Methods: CMECs were isolated from Sprague–Dawley rats. We first examined the effects of AGEs on the proliferation and apoptosis of CMECs and then tested whether protein kinase C (PKC) β blockers could counteract the effects of AGEs. The PKC agonists phorbol 12-myristate 13-acetate (PMA) and PKCβ blockers were also used to verify whether PKC could act independently on CMECs. The receptor for AGEs (RAGE)–small interfering RNA (siRNA) transfection was used to verify the effect of AGEs on PKC. Following the above steps, we explained whether AGEs regulated the CMEC proliferation and early apoptosis through the PKCβ signaling pathway. Proliferation of CMECs was detected using the Cell Counting Kit-8 (CCK-8) assay, and early apoptosis was determined using the Annexin V- Fluorescein Isothiocyanate (FITC)/propidium iodide (PI) double staining. Expression of proliferation and apoptosis-related proteins and PKC phosphorylation were determined by western blotting analysis. Cell cycle distributions were assayed using a BD FACSCalibur cell-sorting system. Results: AGEs facilitated the proliferation of CMECs, upregulated phosphorylated extracellular signal regulated kinase (p-ERK), and accelerated the entry of cells from G1 phase to the S+G2/M phase, which was consistent with the upregulated cyclin D1 by AGEs. AGEs inhibited early apoptosis of CMECs by increasing the expression of survivin and decreasing the expression of cleaved-caspase3. All these effects can be reversed by PKCβ1/2inhibitors. In addition, AGE upregulated the RAGE expression and phosphorylation of PKCβ1/2 in CMECs, while the inhibition of RAGE reversed the phosphorylation, as well as the effects of AGEs on proliferation and apoptosis in CMECs. Conclusion: The study indicated that AGEs facilitated the proliferation and reduced early apoptosis of CMECs via the PKCβ signaling pathway.

وصف الملف: electronic resource

العلاقة: https://jag.journalagent.com/z4/download_fulltext.asp?pdir=anatoljcardiol&un=AJC-21504Test; https://doaj.org/toc/2149-2263Test

الوصول الحر: https://doaj.org/article/2c180dc97885413583197be4c82d1505Test

المؤلفون: Su Buda, Siri Guleng, Celimuge Wu, Jiefang Zhang, Kok-Lim Alvin Yau, Yusheng Ji

المصدر: IEEE Access, Vol 8, Pp 63935-63944 (2020)

مصطلحات موضوعية: Edge computing, vehicular Internet of Things, green ITS, collaborative intelligence, Electrical engineering. Electronics. Nuclear engineering, TK1-9971

الوصف: In order to achieve a greener intelligent transport system (ITS), an efficient collaboration between vehicles is required to manage computation task processing with low latency. In this paper, we propose a collaborative edge computing scheme for vehicular Internet-of-things towards a greener ITS. The proposed scheme uses some vehicles as edge nodes, which are responsible for finding task processor nodes on behalf of a task requester node by considering the end-to-end task response time. The proposed scheme employs a two-stage approach where the first stage enables an efficient networking and computing architecture by forming vehicle clusters based on the edge architecture, and the second stage optimizes offloading tasks based on the architecture. We use realistic computer simulations to compare the proposed scheme with existing baselines, and show its superiority in terms of task offloading performance.

وصف الملف: electronic resource

العلاقة: https://ieeexplore.ieee.org/document/9057430Test/; https://doaj.org/toc/2169-3536Test

الوصول الحر: https://doaj.org/article/f70b82bb00734fc59059a22e9d09800aTest

المؤلفون: Su Buda, Celimuge Wu, Wugedele Bao, Siri Guleng, Jiefang Zhang, Kok-Lim Alvin Yau, Yusheng Ji

المصدر: IEEE Access, Vol 8, Pp 202032-202041 (2020)

مصطلحات موضوعية: Vehicular IoT, blockchain technology, edge computing, fuzzy logic, Electrical engineering. Electronics. Nuclear engineering, TK1-9971

الوصف: In order to enable emerging vehicular Internet of Things (IoT) applications, including fully autonomous driving, more efforts should be done in collecting driving experiences in different road situations. This requires the exchange of information between vehicles as each vehicle has very limited experience. Due to the decentralized feature of vehicular environment, an efficient management of collaborative behaviors among the vehicles becomes particularly important. Blockchain has been attracting great interest recently because it provides a way to reach consensus in decentralized systems. However, existing blockchain systems assume high communication capabilities for vehicles, which is difficult to achieve in a decentralized vehicular environment. Existing studies also assume the existence of networking infrastructure, such as roadside units (RSU). In this paper, we propose a scheme to empower blockchain in vehicular environments without depending on the existing networking infrastructure. The proposed scheme uses a distributed clustering approach to select some vehicles as edge nodes, and the edge nodes maintain the blockchain used to record transactions in a decentralized way. The proposed scheme employs a distributed approach that guides vehicle clustering with the consideration of multiple metrics based on a fuzzy logic algorithm. By using the edge nodes, the proposed scheme solves the communication problem of maintaining a blockchain in a totally decentralized vehicular environment. We use computer simulations to clarify the performance of the proposed scheme in terms of communication performance by comparing it with existing baselines.

وصف الملف: electronic resource

العلاقة: https://ieeexplore.ieee.org/document/9250438Test/; https://doaj.org/toc/2169-3536Test

الوصول الحر: https://doaj.org/article/a49b22fc3b4e4e589fd89039a2854dafTest

المؤلفون: Yang Ye, Shengjie Wu, Lan Su, Xia Sheng, Jiefang Zhang, Bei Wang, Parikshit S. Sharma, Kenneth A. Ellenbogen, Yangang Su, Xueying Chen, Guosheng Fu, Weijian Huang

المصدر: Frontiers in Cardiovascular Medicine, Vol 8 (2021)

مصطلحات موضوعية: cardiac pacing, atrioventricular block (AVB), pacing-induced cardiomyopathy (PICM), heart failure (HF), His bundle pacing (HBP), left bundle branch pacing (LBBP), Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: His bundle pacing (HBP) can reverse left ventricular (LV) remodeling in patients with right ventricular (RV) pacing-induced cardimyopathy (PICM) but may be unable to correct infranodal atrioventricular block (AVB). Left bundle branch pacing (LBBP) results in rapid LV activation and may be able to reliably pace beyond the site of AVB. Our study was conducted to assess the feasibility, safety, and outcomes of permanent LBBP in infranodal AVB and PICM patients. Patients with infranodal AVB and PICM who underwent LBBP for cardiac resynchronization therapy (CRT) were included. Clinical evaluation and echocardiographic and electrocardiographic assessments were recorded at baseline and follow-up. Permanent LBBP upgrade was successful in 19 of 20 patients with a median follow-up duration of 12 months. QRS duration (QRSd) increased from 139.3 ± 28.0 ms at baseline to 176.2 ± 21.4 ms (P < 0.001) with right ventricular pacing (RVP) and was shortened to 120.9 ± 15.2 ms after LBBP (P < 0.001). The mean LBBP threshold was 0.7 ± 0.3 V at 0.4 ms at implant and remained stable during follow-up. The left ventricular ejection fraction (LVEF) increased from 36.3% ± 6.5% to 51.9% ± 13.0% (P < 0.001) with left ventricular end-systolic volume (LVESV) reduced from 180.1 ± 43.5 to 136.8 ± 36.7 ml (P < 0.001) during last follow-up. LBBP paced beyond the site of block, which results in a low pacing threshold with a high success rate in infranodal AVB patients. LBBP improved LV function with stable parameters over the 12 months, making it a reasonable alternative to cardiac resynchronization pacing via a coronary sinus lead in infranodal AVB and PICM patients.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fcvm.2021.674452/fullTest; https://doaj.org/toc/2297-055XTest

الوصول الحر: https://doaj.org/article/5a865142e2fe487282dd1df5e779e839Test

المؤلفون: Jiefang Zhang, Yiwen Pan, Bei Wang, Guosheng Fu

المصدر: Reviews in Cardiovascular Medicine, Vol 23, Iss 3, p 090 (2022)

مصطلحات موضوعية: transcatheter aortic valve replacement, his-purkinje conduction system pacing, left bundle branch block, desynchrony, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Transcatheter aortic valve replacement possesses a high validity for patients with aortic stenosis who are considered high risk for aortic valve replacement surgery, nowadays it is also considered for patients with intermediate risk or even lower risk in certain situations. The incidence of new conduction abnormalities remains to be a tough problem, in particular, left bundle branch block. New-onset left bundle branch block is a major concern despite improvements in valve technology, and it may affect postoperative prognosis. Understanding the anatomical relationship between the conduction system and the aortic root, clarify factors related to the procedure, devices, and patients, might help to reduce the conduction abnormalities. Physiological pacing has emerged as a reasonable pacing strategy for patients with cardiac insufficiency post-valve replacement, especially combined with left bundle branch block. The purpose of this review is to summarize the current opinion on the incidence of new-onset left bundle branch block associated with transcatheter aortic valve replacement, to offer insights into its anatomical and procedural causes, clinical consequences, and more importantly, the prospect of applying physiological pacing as a therapeutic method for these patients.

وصف الملف: electronic resource

العلاقة: https://www.imrpress.com/journal/RCM/23/3/10.31083/j.rcm2303090Test; https://doaj.org/toc/1530-6550Test

الوصول الحر: https://doaj.org/article/2eb067c7c9444d09b84575c40f01ae27Test

المؤلفون: Guosheng Fu, Jiefang Zhang, Min Wang, Xia Sheng, Yiwen Pan

المصدر: International Heart Journal. 2021, 62(1):78