المؤلفون: Li-Li Du, Xianwen Zhao, Yan Wang, Xiaoqin Xu, Cun-zhi Han, Ting Sun, Baoguo Tian, Jie-Xian Jing

المصدر: Asian Pacific Journal of Cancer Prevention. 15:10267-10272

مصطلحات موضوعية: Male, Cancer Research, Pathology, Esophageal Neoplasms, endocrine system diseases, Epidemiology, Gastroenterology, Metastasis, Cohort Studies, Longitudinal Studies, Prospective Studies, Tissue Polypeptide Antigen, Prospective cohort study, Aged, 80 and over, Middle Aged, Esophageal cancer, Prognosis, Oncology, Carcinoma, Squamous Cell, Female, alpha-Fetoproteins, Adult, medicine.medical_specialty, CA-19-9 Antigen, Enzyme-Linked Immunosorbent Assay, Adenocarcinoma, Sensitivity and Specificity, Antigen, Antigens, Neoplasm, Stomach Neoplasms, Internal medicine, Biomarkers, Tumor, Carcinoma, medicine, Humans, Upper gastrointestinal, Antigens, Tumor-Associated, Carbohydrate, neoplasms, Pathological, Serpins, Aged, business.industry, Public Health, Environmental and Occupational Health, Cancer, medicine.disease, digestive system diseases, Carcinoembryonic Antigen, Neoplasm Recurrence, Local, Peptides, business

الوصف: To evaluate the value of combined detection of serum CEA, CA19-9, CA24-2, AFP, CA72-4, SCC, TPA and TPS for the clinical diagnosis of upper gastrointestinal tract (GIT) cancer and to analyze the efficacy of these tumor markers (TMs) in evaluating curative effects and prognosis. A total of 573 patients with upper GIT cancer between January 2004 and December 2007 were enrolled in this study. Serum levels of CEA, CA19-9, CA24-2, AFP, CA72-4, SCC, TPA and TPS were examined preoperatively and every 3 months postoperatively by ELISA. The sensitivity of CEA, CA19-9, CA24-2, AFP, CA72-4, SCC, TPA and TPS were 26.8%, 36.2%, 42.9%, 2.84%, 25.4%, 34.6%, 34.2% and 30.9%, respectively. The combined detection of CEA+CA199+CA242+CA724 had higher sensitivity and specificity in gastric cancer (GC) and cardiac cancer, while CEA+CA199+CA242+SCC was the best combination of diagnosis for esophageal cancer (EC). Elevation of preoperative CEA, CA19-9 and CA24-2, SCC and CA72-4 was significantly associated with pathological types (p

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46b5f133f76778f6485f42d7c86552a4Test
https://doi.org/10.7314/apjcp.2014.15.23.10267Test

المؤلفون: Cun-zhi Han, Wenyuan Wang, Yan Wang, Xin Zhang, Xian-Wen Zhao, Yaoping Li, Jie-Xian Jing

المصدر: Journal of Surgical Oncology. 109:440-444

مصطلحات موضوعية: Oncology, medicine.medical_specialty, Visceral metastasis, Lung, endocrine system diseases, Colorectal cancer, business.industry, Cea mrna, General Medicine, medicine.disease, digestive system diseases, medicine.anatomical_structure, Internal medicine, medicine, Surgery, Clinical significance, In patient, business, neoplasms, Lymph node, Grading (tumors)

الوصف: Objective To explore the clinical significance of expression of CEA mRNA and serum CEA and the related proteins in colorectal cancer (CRC). Methods Blood samples were collected from 370 CRC patients and 350 controls. CEA mRNA was determined by RT-PCR and levels of CEA, CA19-9, CA242, and CA724 were examined with chemiluminescence. Results The positive rate of jointly detecting serum CEA, CA19-9, CA242, and CA724 was significantly higher than CEA mRNA expressions (P

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::7bf1fe35e8efb987e1a97383861cfb4eTest
https://doi.org/10.1002/jso.23503Test

المؤلفون: Cun-zhi Han, Jingang Guo, Xian-wen Zhao, Fumin Liu, Jie-xian Jing, Jirong Cao

المصدر: Chinese Journal of Cancer Research. 9:96-99

مصطلحات موضوعية: Cancer Research, medicine.medical_specialty, Digestive tract cancer, Stomach, Cancer, Biology, medicine.disease, Gastroenterology, chemistry.chemical_compound, medicine.anatomical_structure, Oncology, chemistry, Internal medicine, medicine, High incidence, Nitrite, Esophagus, Risk factor, Cancer death

الوصف: The paper analyzed of investigation datas on the death causes of digestive tract cancer in high-incidence area between 70s and 80s. The results showed that the cancer-adjusted mortalities were 224.14/100000 and 226.66/100000; it was 7 times as high as low-incidence (31.19/100000 and 29.82/100000). In 70s, the cancer death of esophagus, stomach and liver (87.41/100000, 73.93/100000 and 8.59/100000) were 28 times, 10 times and 4 times as high as low-incidence area (3.70/100000, 10.57/100000 and 1.94/100000), respectively (P 0.05). Our results suggest that the nitrate and nitrite contents increase in drinking water and vegetables may be an important risk factor of upper alimentary cancer in high-incidence area.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::5b81a671ff5e133416f3867e6f7a033fTest
https://doi.org/10.1007/bf02974671Test

المؤلفون: Cun-zhi, Han, Li-Li, DU, Jie-xian, Jing, Xian-wen, Zhao, Fu-guo, Tian, Bao-guo, Tian, Hai-ming, Wu

المصدر: Zhonghua zhong liu za zhi [Chinese journal of oncology]. 33(3)

مصطلحات موضوعية: Adenoma, Adult, Hyperplasia, Carcinoma, Breast Neoplasms, Exons, Middle Aged, Gene Frequency, Humans, Point Mutation, Receptors, Leptin, Female, Breast, Obesity, Aged

الوصف: The aim of this study was to investigate the relationship of the mutations of leptin receptor gene exon 4, exon 6, exon9, and exon20 with the tumorigenesis of breast cancer.Genomic DNA was extracted from breast cancer tissues of 155 patients, benign lesions of 56 patients and normal tissues and blood samples from 100 health control subjects. The leptin receptor genes were assayed with polymerase chain reaction (PCR) amplification and direct sequence analysis.Nucleotide substitutions no mutations were found at exon 4, and nucleotide substitutions occurred at codon 1029 in exon 9, no significant difference among the three groups (P = 0.574). The nucleotide substitutions at codon 668 in exon 6 resulted in Gln223Arg polymorphisms. The occurring frequencies of GG, GA, AA in breast cancer, breast benign lesions tissues and health tissues control group were 70.9% and 17.4%, 12.3%; 80.4%, 14.3% and 5.4%; and 81.0%, 16.0%, and 3.0%, respectively. Alleles of G and A in the three groups were 79.1% and 20.8%, 87.5% and 12.5%, and 89.0% and 11.0%, respectively. Compared the Gln223Arg genotype with the three allele groups, there were significant differences (χ(2) = 16.11, P0.005 and χ(2) = 11.41, P0.01), respectively. The nucleotide substitutions at codon 3057 in exon 20 resulted in Pro1019Pro polymorphisms. The occurrence frequencies of GG, GA, AA in the breast cancer, benign disease and health control groups were 11.6%, 30.3% and 56.1%; 32.1%, 44.0% and 28.5%; and 32.0%, 45.0% And 23.0%, respectively. Alleles of G and A in the three groups were 26.8% and 73.2%, 51.8% and 48.2%, and 54.5% and 45.5%, respectively. There are significant differences among the three groups (χ(2) = 6.56, P0.03 and χ(2) = 5.45, P0.05), respectively. Nucleotide substitutions occurred at relatively high frequencies at exon 6 and exon 20 in obese and overweight breast cancer patients compared with those in normal weight breast cancer patients, there were significant differences (P0.05 and P0.01).Our findings show that there is no relationship between the variations of leptin receptor gene exon 9 and tumorigenesis of breast cancer. The variation rate of leptin receptor gene exon 6 and exon 20 are significantly increased in the obese and overweight breast cancer patients.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::2ee9536cfe47232f7cec60b31a739391Test
https://pubmed.ncbi.nlm.nih.gov/21575521Test

المؤلفون: Ji-wen, Song, Li-li, DU, Xian-wen, Zhao, Jie-xian, Jing, Cun-zhi, Han, Yu, Cui, Jian-wu, Liu, Hai-long, Hao, Zhen-guo, Wang, Zhen-guo, Mi

المصدر: Zhonghua zhong liu za zhi [Chinese journal of oncology]. 31(4)

مصطلحات موضوعية: Adult, Aged, 80 and over, Male, Carcinoma, Transitional Cell, Adolescent, Keratin-18, Nuclear Proteins, Middle Aged, Prognosis, Sensitivity and Specificity, Young Adult, Urinary Bladder Neoplasms, Biomarkers, Tumor, Humans, Female, Neoplasm Metastasis, Neoplasm Recurrence, Local, Child, Carcinoma, Renal Cell, Aged, Follow-Up Studies, Neoplasm Staging

الوصف: To evaluate the expression and clinical significance of urinary nuclear matrix protein (NMP22) and cytokeratin 18 (CK18) for transitional cell carcinoma of the bladder.Urinary NMP22 and CK18 levels of 293 patients with transitional cell carcinoma of the bladder, 400 patients with non-transitional cell carcinoma of the bladder, and 105 bladder benign disease were analysed by enzyme-linked-immunosorbent assay (ELISA).The levels of urinary NMP22 and CK18 in the patients with transitional cell carcinoma of the bladder (M = 17.3 U/ml, M(CK18) = 484.2 U/L) were significantly higher than those in the non-transitional cell carcinoma of the bladder (M = 6.8 U/ml, M(CK18) = 156.0 U/L) and the benign disease group (M(NMP22) = 2.3 U/ml, M(CK18) = 66.6 U/L) (P0.001). The sensitivity and specificity of urinary NMP22 and CK18 were 79.2%, 88.6% and 78.2%, 82.9%, respectively, for transitional cell carcinoma of the bladder before any treatment. The joint sensitivity of the two markers was 91.7%. The NMP22 and CK18 levels were significantly lower in the recovered patients after surgical operation (P0.01), while in patients with recurrence or metastasis the levels of the markers were significantly higher (P0.01). There was a significant relationship between NMP22 and CK18, (r = 0.689, P0.01). The levels of urinary nmp22 and CK18 were significantly different among pathological grade G1, G2, G3, and stage Ta, T1, T2, T3 (P0.01).NMP22 and CK18 are useful tumor marker for diagnosis of transitional cell carcinoma of the bladder and for monitoring the state of illness. The joint use of the two markers can improve the sensitivity of cancer detection. NMP22 and CK18 may become a new class of tumor markers, and to be the basis for development of a new assay with an increased efficacy for the detection and treatment of bladder cancer.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::fbe1b6014fa4c08e275935adce30c421Test
https://pubmed.ncbi.nlm.nih.gov/19615282Test

المؤلفون: Jie-xian Jing, Xiu-ying Liu, Jing Shi, Fu-guo Tian, Xian-wen Zhao, Cun-zhi Han, Baoguo Tian, Li-Li Du, Li-Jiao Zhang

المصدر: Biological trace element research. 126(1-3)

مصطلحات موضوعية: Adult, Leptin, medicine.medical_specialty, China, Apolipoprotein B, Genotype, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Clinical Biochemistry, Breast Neoplasms, Biochemistry, Body Mass Index, Inorganic Chemistry, Breast cancer, Waist–hip ratio, Internal medicine, medicine, Humans, Life Style, Aged, Leptin receptor, Polymorphism, Genetic, biology, Waist-Hip Ratio, Insulin, Biochemistry (medical), General Medicine, Middle Aged, medicine.disease, Lipids, Endocrinology, biology.protein, Receptors, Leptin, Female, Body mass index

الوصف: We evaluated the relationship among the leptin receptor (LEPR) gene Gln223Arg polymorphism, body mass index (BMI), waist and hip circumference ratio (WHR), dietary structure, lifestyle, and other biomarkers with breast cancer and determined whether they could be effective for the prevention and treatment of breast cancer. The Gln223Arg polymorphisms in the LEPR gene were investigated in blood deoxyribonucleic acid (DNA) available for 240 breast cancer cases and 500 controls. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism. Leptin, insulin were determined by enzyme-linked immunosorbent assays. We found that the serum levels of leptin, insulin, triglyceride (TG), free cholesterol (FCH), apolipoprotain (APO) A1, and BMI were significantly higher in breast cancer cases than the controls, while physical activity was clearly less in breast cancer cases (P < 0.02 approximately P < 0.001, respectively). Moreover, there were significant association between the Gln223Arg genotype and breast cancer risk; homozygotes for AA and heterozygotes for AG,AG + GG genotypes had been proved to increase the risk of breast cancer, and their corresponding odds ratio were 7.14 (95% confidence interval [CI] = 1.92-25.64), 1.33(95% CI = 1.03-2.70), and 2.04 (95% CI = 1.09-3.82). Interestingly, logistic regression analysis showed that LEPR gene Gln223Arg polymorphism and elevated leptin, insulin, TG, FCH, APOA1, WHR, and reduced APOB increased the risk of developing breast cancer, respectively. And, it also suggested that LEPR gene Gln223Arg polymorphisms, elevated leptin, insulin, TG, FCH, APOA1, WHR, and reduced APOB should play a major role in the development of breast cancer.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5d23dfcc18b5d3a027d6f7bad3470c6Test
https://pubmed.ncbi.nlm.nih.gov/18668212Test

المؤلفون: Hui-Qing, Chen, Cun-Zhi, Han, Li-Li, Du, Yu, Cui, Dong-Zhi, Pang, Jie-Xian, Jing, Xian-Wen, Zhao, Bao-Guo, Tian, Zhen-Guo, Mi

المصدر: Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine].

مصطلحات موضوعية: Adult, Aged, 80 and over, Male, Carcinoma, Transitional Cell, Adolescent, Urinary Bladder Neoplasms, Biomarkers, Tumor, Humans, Nuclear Proteins, Female, Middle Aged, Child, Aged

الوصف: To evaluate the urinary nuclear matrix protein (NMP22) as an adjuvant diagnostic index for transitional cell carcinoma of urinary tract and monitoring the state of disease.Urinary samples were collected from 262 patients with transitional cell carcinoma, 198 non-transitional cell carcinoma of the urinary tract and 65 patients with benign diseases. Urinary NMP22 concentration was determined through enzyme linked immunosorbent assay (ELISA).The urinary NMP22 concentration had significant difference among the three groups (Kruskal Wallis, chi(2) = 197.17 P0.001). The detection sensitivity and specificity of urinary NMP22 to transitional cell carcinoma were 71.37% and 87.69% respectively. The NMP22 concentration showed significant difference among three groups divided according to the pathological grade (Kruskal-Wallis test, chi(2) = 34.06 P0.01). The NMP22 concentration was significant lower in the recovery patients after the operation than the peoples of pre-operation and recurrence (Kruskal-Wallis test, chi(2) = 37.53, P0.001).MP22 is a helpful tumor marker for the diagnosis of transitional cell carcinoma and monitoring the state of illness with increased efficacy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::d66dc06aa7f30cf5c79e099313589fe0Test
https://pubmed.ncbi.nlm.nih.gov/17767866Test

المؤلفون: Cun-zhi, Han, Jing, Shi, Li-li, Du, Jie-xian, Jing, Xian-wen, Zhao, Bao-guo, Tian, Fu-guo, Tian, Xiu-ying, Liu, Zhong-shu, Zhang, Jin, Zhang

المصدر: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi. 28(2)

مصطلحات موضوعية: Leptin, Risk, Polymorphism, Genetic, Humans, Receptors, Leptin, Breast Neoplasms, Enzyme-Linked Immunosorbent Assay, Female, Genetic Predisposition to Disease, Lipids

الوصف: To evaluate the association between serum level of leptin and leptin receptor gene (LEPR) polymorphism and patients with breast cancer.LEPR G1n223Arg polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism in 94 patients with breast cancer and 128 healthy controls. The level of leptin were analyzed by enzyme linked immunosorbent assay.In univariate regression analyses, we found serum level of leptin and LEPR Gin223Arg genotype polymorphism were significantly higrer than those of the controls (P0.05-0.001, respectively). Through multivariable analyses, we found that increased risk estimates for breast cancer were among those with leptin level (OR = 1.53, 95% CI: 1.13-2.07, P = 0.006), LEPR Gin223Arg genotype (OR = 4.87, 95%CI:1.30-18.22, P = 0.019), WHR (OR = 3.68, 95% CI: 1.34-10.11, P = 0.011).Results from this study suggested that LEPR Gln233Agr polymorphism, the elevated WHR and serum level of leptin might be correlated with increased risk of breast cancer.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::a46c7b92081fa0c576cf4cc3a1a9d9c8Test
https://pubmed.ncbi.nlm.nih.gov/17649682Test

المؤلفون: Xian-wen, Zhao, Bo, Jiang, Cun-zhi, Han, Jie-xian, Jing

المصدر: Zhonghua zhong liu za zhi [Chinese journal of oncology]. 27(5)

مصطلحات موضوعية: Adult, Male, CA-19-9 Antigen, Middle Aged, Sensitivity and Specificity, Carcinoembryonic Antigen, Lymphatic Metastasis, Biomarkers, Tumor, Humans, Antigens, Tumor-Associated, Carbohydrate, Female, Neoplasm Invasiveness, Lymph Nodes, Colorectal Neoplasms, Aged

الوصف: To investigate the diagnostic value and clinical significance of serum tumor markers CEA, CA19-9 and CA242 in patients with colorectal cancer.The serum levels of CEA, CA19-9 and CA242 were determined by ELISA before surgery in 134 patients with colorectal cancer and in 200 healthy people as a control.The CEA, CA19-9 and CA242 levels in patients were significantly higher than those in controls (P0.01, respectively). The sensitivity of CEA and CA242 for colorectal cancer diagnosis was higher than that of CA19-9, and the combined sensitivity of CEA + CA242 and CEA + CA242 + CA19-9 were higher than that of single item or the other two combinations (CEA + CA19-9 and CA19-9 + CA242). In Dukes stages A, B, C and D, serum levels and sensitivity of the three tumor markers were significantly and successively increased. In the cases with lymph node metastasis, levels of the three tumor markers were significantly increased. The markers levels were also significantly and successively increased along with the extent of cancer infiltration.The results indicate that the combined use of CEA and CA242 or the three markers is an useful adjuvant diagnostic measure for colorectal cancer, and is helpful in the evaluation of lymph node metastasis, degree of invasion and Dukes staging in cancer treatment.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::67da084903216d7cd606cc7c02d4eb88Test
https://pubmed.ncbi.nlm.nih.gov/15996321Test

المؤلفون: Xiu-ying Liu, Xian-wen Zhao, Li-Li Du, Xuan Yang, Hong-Tao Zhang, Jie-xian Jing, Baoguo Tian, Cun-zhi Han

المصدر: Endocrine. 26(1)

مصطلحات موضوعية: Adult, Leptin, medicine.medical_specialty, China, Apolipoprotein B, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Breast Neoplasms, Endocrinology, Breast cancer, Internal medicine, Diabetes mellitus, medicine, Humans, Insulin, Risk factor, Triglycerides, Apolipoproteins B, biology, Apolipoprotein A-I, business.industry, Middle Aged, medicine.disease, Obesity, Lipids, Cholesterol, Logistic Models, biology.protein, lipids (amino acids, peptides, and proteins), Female, Breast disease, business

الوصف: Epidemiological studies have found obesity to be a risk factor for women's breast cancer. The present study was to investigate whether there is a relationship between serum levels of leptin, insulin, and lipids and breast cancer incidence, in order to find experimental evidence that would be helpful in the diagnosis and prevention of breast cancer. Blood samples were collected from 130 patients with mammary disease and 103 healthy control subjects. Serum leptin, insulin, and lipids were determined by radioimmunoassay (RIA), enzyme-linked immunosorbent assays (ELISA), and Biochemistry Auto-analyzer, respectively. The data analysis was performed by use of the SPSS10.0 computer software. We found that the serum levels of leptin, insulin, and triglyceride (TG) were clearly higher in patients with breast cancer than in patients with benign breast disease and healthy controls, while serum HDL-C levels were lower in breast cancer patients (p0.03). Moreover, serum leptin levels were significantly correlated with BMI (body mass index) among three groups, whereas serum insulin levels were unrelated to BMI among three groups. Furthermore, the serum levels of leptin and insulin were not associated with menopausal status in patients with mammary disease (p0.05); however, the serum levels of F-Chol, T-Chol, TG, LDL-C, and APOB were significant higher in postmenopausal cases than those in premenopausal cases (p0.025). Interestingly, logistic regression analysis showed that subjects with elevated serum levels of leptin, insulin, TG, APOA1, and reduced level of serum HDL-C displayed increased risk of developing breast cancer than those with the normal levels, respectively. In conclusion, the present study suggested that aberrant serum levels of leptin, insulin, and lipids might play an important role in carcinogenesis of breast cancer. The elevated serum levels of leptin, insulin, TG, APOA1, and reduced level of serum HDL-C may be correlated with increased risk of breast cancer, suggesting that one way of preventing breast cancer would be carried out by controlling the intake of food.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b86c42c0e6ac24f058a8eeb712f60256Test
https://pubmed.ncbi.nlm.nih.gov/15805581Test