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1دورية أكاديمية
المؤلفون: Drake W. Phelps, Anika I. Palekar, Haleigh E. Conley, Giuliano Ferrero, Jacob H. Driggers, Keith E. Linder, Seth W. Kullman, David M. Reif, M. Katie Sheats, Jamie C. DeWitt, Jeffrey A. Yoder
المصدر: Journal of Immunotoxicology, Vol 20, Iss 1 (2023)
مصطلحات موضوعية: Innate immunity, neutrophils, per- and polyfluoroalkyl substances (PFAS), reactive oxygen species, zebrafish, Immunologic diseases. Allergy, RC581-607, Toxicology. Poisons, RA1190-1270
الوصف: AbstractPer- and polyfluoroalkyl substances (PFASs) are used in a multitude of processes and products, including nonstick coatings, food wrappers, and fire-fighting foams. These chemicals are environmentally-persistent, ubiquitous, and can be detected in the serum of 98% of Americans. Despite evidence that PFASs alter adaptive immunity, few studies have investigated their effects on innate immunity. The report here presents results of studies that investigated the impact of nine environmentally-relevant PFASs [e.g. perfluorooctanoic acid (PFOA), perfluorooctane sulfonic acid potassium salt (PFOS-K), perfluorononanoic acid (PFNA), perfluorohexanoic acid (PFHxA), perfluorohexane sulfonic acid (PFHxS), perfluorobutane sulfonic acid (PFBS), ammonium perfluoro(2-methyl-3-oxahexanoate) (GenX), 7H-perfluoro-4-methyl-3,6-dioxa-octane sulfonic acid (Nafion byproduct 2), and perfluoromethoxyacetic acid sodium salt (PFMOAA-Na)] on one component of the innate immune response, the neutrophil respiratory burst. The respiratory burst is a key innate immune process by which microbicidal reactive oxygen species (ROS) are rapidly induced by neutrophils in response to pathogens; defects in the respiratory burst can increase susceptibility to infection. The study here utilized larval zebrafish, a human neutrophil-like cell line, and primary human neutrophils to ascertain whether PFAS exposure inhibits ROS production in the respiratory burst. It was observed that exposure to PFHxA and GenX suppresses the respiratory burst in zebrafish larvae and a human neutrophil-like cell line. GenX also suppressed the respiratory burst in primary human neutrophils. This report is the first to demonstrate that these PFASs suppress neutrophil function and support the utility of employing zebrafish larvae and a human cell line as screening tools to identify chemicals that may suppress human immune function.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Alex Dornburg, Rittika Mallik, Zheng Wang, Moisés A. Bernal, Brian Thompson, Elspeth A. Bruford, Daniel W. Nebert, Vasilis Vasiliou, Laurel R. Yohe, Jeffrey A. Yoder, Jeffrey P. Townsend
المصدر: Human Genomics, Vol 16, Iss 1, Pp 1-26 (2022)
الوصف: Abstract Following the draft sequence of the first human genome over 20 years ago, we have achieved unprecedented insights into the rules governing its evolution, often with direct translational relevance to specific diseases. However, staggering sequence complexity has also challenged the development of a more comprehensive understanding of human genome biology. In this context, interspecific genomic studies between humans and other animals have played a critical role in our efforts to decode human gene families. In this review, we focus on how the rapid surge of genome sequencing of both model and non-model organisms now provides a broader comparative framework poised to empower novel discoveries. We begin with a general overview of how comparative approaches are essential for understanding gene family evolution in the human genome, followed by a discussion of analyses of gene expression. We show how homology can provide insights into the genes and gene families associated with immune response, cancer biology, vision, chemosensation, and metabolism, by revealing similarity in processes among distant species. We then explain methodological tools that provide critical advances and show the limitations of common approaches. We conclude with a discussion of how these investigations position us to gain fundamental insights into the evolution of gene families among living organisms in general. We hope that our review catalyzes additional excitement and research on the emerging field of comparative genomics, while aiding the placement of the human genome into its existentially evolutionary context.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/1479-7364Test
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3دورية أكاديمية
المصدر: Journal of Immunotoxicology, Vol 21, Iss 1 (2024)
مصطلحات موضوعية: Per- and polyfluoroalkyl substances, PFAS, innate immunity, ecotoxicology, zebrafish, immunotoxicology, Immunologic diseases. Allergy, RC581-607, Toxicology. Poisons, RA1190-1270
الوصف: AbstractPer- and polyfluoroalkyl substances (PFASs) are a large class of compounds used in a variety of processes and consumer products. Their unique chemical properties make them ubiquitous and persistent environmental contaminants while also making them economically viable and socially convenient. To date, several reviews have been published to synthesize information regarding the immunotoxic effects of PFASs on the adaptive immune system. However, these reviews often do not include data on the impact of these compounds on innate immunity. Here, current literature is reviewed to identify and incorporate data regarding the effects of PFASs on innate immunity in humans, experimental models, and wildlife. Known mechanisms by which PFASs modulate innate immune function are also reviewed, including disruption of cell signaling, metabolism, and tissue-level effects. For PFASs where innate immune data are available, results are equivocal, raising additional questions about common mechanisms or pathways of toxicity, but highlighting that the innate immune system within several species can be perturbed by exposure to PFASs. Recommendations are provided for future research to inform hazard identification, risk assessment, and risk management practices for PFASs to protect the immune systems of exposed organisms as well as environmental health.
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: John Efromson, Giuliano Ferrero, Aurélien Bègue, Thomas Jedidiah Jenks Doman, Clay Dugo, Andi Barker, Veton Saliu, Paul Reamey, Kanghyun Kim, Mark Harfouche, Jeffrey A. Yoder
المصدر: PLoS ONE, Vol 18, Iss 12 (2023)
وصف الملف: electronic resource
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5دورية أكاديمية
المؤلفون: Dustin J. Wcisel, J. Thomas Howard, Jeffrey A. Yoder, Alex Dornburg
المصدر: BMC Evolutionary Biology, Vol 20, Iss 1, Pp 1-10 (2020)
مصطلحات موضوعية: BUSCO ortholog assembly, Cetacean and teleost fish phylogeny, Missing data visualization, Transcriptome, Concatenated alignment, Evolution, QH359-425
الوصف: Abstract Background Advances in next-generation sequencing technologies have reduced the cost of whole transcriptome analyses, allowing characterization of non-model species at unprecedented levels. The rapid pace of transcriptomic sequencing has driven the public accumulation of a wealth of data for phylogenomic analyses, however lack of tools aimed towards phylogeneticists to efficiently identify orthologous sequences currently hinders effective harnessing of this resource. Results We introduce TOAST, an open source R software package that can utilize the ortholog searches based on the software Benchmarking Universal Single-Copy Orthologs (BUSCO) to assemble multiple sequence alignments of orthologous loci from transcriptomes for any group of organisms. By streamlining search, query, and alignment, TOAST automates the generation of locus and concatenated alignments, and also presents a series of outputs from which users can not only explore missing data patterns across their alignments, but also reassemble alignments based on user-defined acceptable missing data levels for a given research question. Conclusions TOAST provides a comprehensive set of tools for assembly of sequence alignments of orthologs for comparative transcriptomic and phylogenomic studies. This software empowers easy assembly of public and novel sequences for any target database of candidate orthologs, and fills a critically needed niche for tools that enable quantification and testing of the impact of missing data. As open-source software, TOAST is fully customizable for integration into existing or novel custom informatic pipelines for phylogenomic inference. Software, a detailed manual, and example data files are available through github carolinafishes.github.io
وصف الملف: electronic resource
العلاقة: http://link.springer.com/article/10.1186/s12862-020-01603-wTest; https://doaj.org/toc/1471-2148Test
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6دورية أكاديمية
المؤلفون: Drake W. Phelps, Ashley A. Fletcher, Ivan Rodriguez-Nunez, Michele R. Balik-Meisner, Debra A. Tokarz, David M. Reif, Dori R. Germolec, Jeffrey A. Yoder
المصدر: Journal of Immunotoxicology, Vol 17, Iss 1, Pp 94-104 (2020)
مصطلحات موضوعية: chemical screen, high throughput, phagocyte, reactive oxygen species (ros), polycyclic aromatic hydrocarbons (pah), endocrine disrupting compounds (edc), lead, Immunologic diseases. Allergy, RC581-607, Toxicology. Poisons, RA1190-1270
الوصف: Currently, assessment of the potential immunotoxicity of a given agent involves a tiered approach for hazard identification and mechanistic studies, including observational studies, evaluation of immune function, and measurement of susceptibility to infectious and neoplastic diseases. These studies generally use costly low-throughput mammalian models. Zebrafish, however, offer an excellent alternative due to their rapid development, ease of maintenance, and homology to mammalian immune system function and development. Larval zebrafish also are a convenient model to study the innate immune system with no interference from the adaptive immune system. In this study, a respiratory burst assay (RBA) was utilized to measure reactive oxygen species (ROS) production after developmental xenobiotic exposure. Embryos were exposed to non-teratogenic doses of chemicals and at 96 h post-fertilization, the ability to produce ROS was measured. Using the RBA, 12 compounds with varying immune-suppressive properties were screened. Seven compounds neither suppressed nor enhanced the respiratory burst; five reproducibly suppressed global ROS production, but with varying potencies: benzo[a]pyrene, 17β-estradiol, lead acetate, methoxychlor, and phenanthrene. These five compounds have all previously been reported as immunosuppressive in mammalian innate immunity assays. To evaluate whether the suppression of ROS by these compounds was a result of decreased immune cell numbers, flow cytometry with transgenic zebrafish larvae was used to count the numbers of neutrophils and macrophages after chemical exposure. With this assay, benzo[a]pyrene was found to be the only chemical that induced a change in the number of immune cells by increasing macrophage but not neutrophil numbers. Taken together, this work demonstrates the utility of zebrafish larvae as a vertebrate model for identifying compounds that impact innate immune function at non-teratogenic levels and validates measuring ROS production and phagocyte numbers as metrics for monitoring how xenobiotic exposure alters the innate immune system.
وصف الملف: electronic resource
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7دورية أكاديمية
المؤلفون: Emma G. Stafford, Amanda Kortum, Aude Castel, Lauren Green, Jeanie Lau, Peter J. Early, Karen R. Muñana, Christopher L. Mariani, Jeffrey A. Yoder, Natasha J. Olby
المصدر: Journal of Veterinary Internal Medicine, Vol 33, Iss 5, Pp 2175-2182 (2019)
مصطلحات موضوعية: immunofluorescent assay, meningoencephalitis, MUE, NMDA receptor encephalitis, Veterinary medicine, SF600-1100
الوصف: Abstract Background Presumed autoimmune diseases affecting the central nervous system (CNS) of dogs are common. In people, antibodies against neuronal cell surface antigens that are associated with a wide variety of neurological syndromes have been identified. The presence of cerebrospinal fluid (CSF) autoantibodies that target neuronal cell surface proteins has not been reported in dogs with neurologic disorders. Objectives Autoantibodies to neuronal cell surface antigens can be found in the CSF of dogs with inflammatory CNS disease. Our aim was to determine whether 6 neuronal cell surface autoantibodies were present in the CSF of dogs diagnosed with inflammatory and noninflammatory CNS disease. Animals Client‐owned dogs with CNS disease and complete diagnostic evaluation including magnetic resonance imaging and CSF analysis were included. One healthy dog was included as a negative control. Methods Cerebrospinal fluid was tested for 6 antigenic targets with a commercially available indirect immunofluorescence assay test kit. Results There were 32 dogs with neurological disease, 19 diagnosed with inflammatory disease (encephalitis and meningitis), 10 with noninflammatory disease (neoplasia, intervertebral disk disease, degenerative myelopathy, and epilepsy), 2 with no diagnosis, and 1 with neoplasia and meningoencephalitis. Anti‐N‐methyl‐d‐aspartate receptor 1 (NMDAR1) antibodies were detected in 3 dogs (3/32; 9.38%). All 3 dogs responded to treatment of meningoencephalomyelitis of unknown etiology (MUE). Conclusions and Clinical Importance Further evaluation of the prevalence and clinical relevance of CSF and serum antibodies to neuronal cell surface antigens is warranted. Defining antigenic targets associated with encephalitis in dogs might allow diagnostic categorization of MUE antemortem.
وصف الملف: electronic resource
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8دورية أكاديمية
المصدر: Brain and Behavior, Vol 9, Iss 12, Pp n/a-n/a (2019)
مصطلحات موضوعية: dorsal laminectomy dissection, dorsal root ganglia cell culture, fura‐2AM, ratiometric calcium imaging, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
الوصف: Abstract Introduction Rodent primary sensory neurons are commonly used for studying itch and pain neurophysiology, but translation from rodents to larger mammals and humans is not direct and requires further validation to make correlations. Methods This study developed a primary canine sensory neuron culture from dorsal root ganglia (DRG) excised from cadaver dogs. Additionally, the canine DRG cell cultures developed were used for single‐cell ratiometric calcium imaging, with the activation of neurons to the following pruritogenic and algogenic substances: histamine, chloroquine, canine protease‐activated receptor 2 (PAR2) activating peptide (SLIGKT), compound 48/80, 5‐hydroxytryptamine receptor agonist (5‐HT), bovine adrenal medulla peptide (BAM8‐22), substance P, allyl isothiocyanate (AITC), and capsaicin. Results This study demonstrates a simple dissection and rapid processing of DRG collected from canine cadavers used to create viable primary sensory neuron cultures to measure responses to pruritogens and algogens. Conclusion Ratiometric calcium imaging demonstrated that small‐diameter canine sensory neurons can be activated by multiple stimuli, and a single neuron can react to both a pruritogenic stimulation and an algogenic stimulation.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/2162-3279Test
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9دورية أكاديمية
المؤلفون: Radhika N. Shah, Ivan Rodriguez-Nunez, Donna D. Eason, Robert N. Haire, Julien Y. Bertrand, Valērie Wittamer, David Traver, Shila K. Nordone, Gary W. Litman, Jeffrey A. Yoder
المصدر: Advances in Hematology, Vol 2012 (2012)
مصطلحات موضوعية: Diseases of the blood and blood-forming organs, RC633-647.5
الوصف: The novel immune-type receptors (NITRs), which have been described in numerous bony fish species, are encoded by multigene families of inhibitory and activating receptors and are predicted to be functional orthologs to the mammalian natural killer cell receptors (NKRs). Within the zebrafish NITR family, nitr9 is the only gene predicted to encode an activating receptor. However, alternative RNA splicing generates three distinct nitr9 transcripts, each of which encodes a different isoform. Although nitr9 transcripts have been detected in zebrafish lymphocytes, the specific hematopoietic lineage(s) that expresses Nitr9 remains to be determined. In an effort to better understand the role of NITRs in zebrafish immunity, anti-Nitr9 monoclonal antibodies were generated and evaluated for the ability to recognize the three Nitr9 isoforms. The application of these antibodies to flow cytometry should prove to be useful for identifying the specific lymphocyte lineages that express Nitr9 and may permit the isolation of Nitr9-expressing cells that can be directly assessed for cytotoxic (e.g., NK) function.
وصف الملف: electronic resource
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10دورية أكاديمية
المؤلفون: Tyler A. Allen, Mark M. Cullen, Nathan Hawkey, Hiroyuki Mochizuki, Lan Nguyen, Elyse Schechter, Luke Borst, Jeffrey A. Yoder, Jennifer A. Freedman, Steven R. Patierno, Ke Cheng, William C. Eward, Jason A. Somarelli
المصدر: Frontiers in Oncology, Vol 11 (2021)
مصطلحات موضوعية: angiopellosis, circulating tumor cell cluster, tumor cell extravasation, cancer exodus hypothesis, metastasis, osteosarcoma, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
الوصف: Metastasis is a multistep process in which cells must detach, migrate/invade local structures, intravasate, circulate, extravasate, and colonize. A full understanding of the complexity of this process has been limited by the lack of ability to study these steps in isolation with detailed molecular analyses. Leveraging a comparative oncology approach, we injected canine osteosarcoma cells into the circulation of transgenic zebrafish with fluorescent blood vessels in a biologically dynamic metastasis extravasation model. Circulating tumor cell clusters that successfully extravasated the vasculature as multicellular units were isolated under intravital imaging (n = 6). These extravasation-positive tumor cell clusters sublines were then molecularly profiled by RNA-Seq. Using a systems-level analysis, we pinpointed the downregulation of KRAS signaling, immune pathways, and extracellular matrix (ECM) organization as enriched in extravasated cells (p < 0.05). Within the extracellular matrix remodeling pathway, we identified versican (VCAN) as consistently upregulated and central to the ECM gene regulatory network (p < 0.05). Versican expression is prognostic for a poorer metastasis-free and overall survival in patients with osteosarcoma. Together, our results provide a novel experimental framework to study discrete steps in the metastatic process. Using this system, we identify the versican/ECM network dysregulation as a potential contributor to osteosarcoma circulating tumor cell metastasis.
العلاقة: https://www.frontiersin.org/articles/10.3389/fonc.2021.641187/fullTest; https://doaj.org/toc/2234-943XTest; https://doaj.org/article/529bbd20dab947d0902ce121f8d74ea4Test
الإتاحة: https://doi.org/10.3389/fonc.2021.641187Test
https://doaj.org/article/529bbd20dab947d0902ce121f8d74ea4Test
النص الكامل