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1دورية أكاديمية
المؤلفون: William R Berrington, Chhatra B Kunwar, Kapil Neupane, Susan J F van den Eeden, James C Vary, Glenna J Peterson, Richard D Wells, Annemieke Geluk, Deanna A Hagge, Thomas R Hawn
المصدر: PLoS Neglected Tropical Diseases, Vol 8, Iss 11, p e3263 (2014)
مصطلحات موضوعية: Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270
الوصف: Leprosy is characterized by polar clinical, histologic and immunological presentations. Previous immunologic studies of leprosy polarity were limited by the repertoire of cytokines known at the time.We used a candidate gene approach to measure mRNA levels in skin biopsies from leprosy lesions. mRNA from 24 chemokines and cytokines, and 6 immune cell type markers were measured from 85 Nepalese leprosy subjects. Selected findings were confirmed with immunohistochemistry.Expression of three soluble mediators (CCL18, CCL17 and IL-10) and one macrophage cell type marker (CD14) was significantly elevated in lepromatous (CCL18, IL-10 and CD14) or tuberculoid (CCL17) lesions. Higher CCL18 protein expression by immunohistochemistry and a trend in increased serum CCL18 in lepromatous lesions was observed. No cytokines were associated with erythema nodosum leprosum or Type I reversal reaction following multiple comparison correction. Hierarchical clustering suggested that CCL18 was correlated with cell markers CD209 and CD14, while neither CCL17 nor CCL18 were highly correlated with classical TH1 and TH2 cytokines.Our findings suggest that CCL17 and CCL18 dermal expression is associated with leprosy polarity.
وصف الملف: electronic resource
العلاقة: http://europepmc.org/articles/PMC4238987?pdf=renderTest; https://doaj.org/toc/1935-2727Test; https://doaj.org/toc/1935-2735Test
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2دورية أكاديمية
المؤلفون: Phillip I. Tarr, Sima S. Bilge, James C. Vary, Rebecca L. Habeeb, Teresa R. Ward, Michael R, Thomas E. Besser
المساهمون: The Pennsylvania State University CiteSeerX Archives
الوصف: Updated information and services can be found at
وصف الملف: application/pdf
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3دورية أكاديمية
المؤلفون: J. Clin Microbiol, James C. Vary, Phillip I. Tarr, Patricia M. Desmarchelier, Sima S. Bilge, Narelle Fegan
المساهمون: The Pennsylvania State University CiteSeerX Archives
الوصف: This article cites 17 articles, 9 of which can be accessed free at
وصف الملف: application/pdf
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4دورية أكاديمية
المؤلفون: S S Bilge, J C Vary, S F Dowell, P I Tarr, Sima S. Bilge, James C. Vary, Scott F. Dowell, Phillip I. Tarr
المساهمون: The Pennsylvania State University CiteSeerX Archives
الوصف: Role of the Escherichia coli O157:H7 O side chain in adherence and analysis of an rfb locus.
وصف الملف: application/pdf
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5
المؤلفون: Richard D. Wells, Thomas R. Hawn, James C. Vary, Saraswoti Khadge, William R. Berrington, Deanna A. Hagge, Javeed A. Shah, Chhatra B. Kunwar, Glenna J. Peterson
المصدر: The Journal of infectious diseases. 213(7)
مصطلحات موضوعية: 0301 basic medicine, Adult, Genotype, Interleukin-1 receptor, Polymorphism, Single Nucleotide, 03 medical and health sciences, Major Articles and Brief Reports, Immune system, Nepal, Leprosy, Immunology and Allergy, Skin immunity, Humans, Genetic Predisposition to Disease, Prospective Studies, Mycobacterium leprae, Skin, biology, TOLLIP, Intracellular Signaling Peptides and Proteins, Interleukin, Gene Expression Regulation, Bacterial, biology.organism_classification, Interleukin 1 Receptor Antagonist Protein, 030104 developmental biology, Infectious Diseases, Interleukin 1 receptor antagonist, Case-Control Studies, Immunology, Toll-Interacting Protein
الوصف: Leprosy is a chronic disease characterized by skin and peripheral nerve pathology and immune responses that fail to control Mycobacterium leprae. Toll-interacting protein (TOLLIP) regulates Toll-like receptor (TLR) and interleukin 1 receptor (IL-1R) signaling against mycobacteria. We analyzed messenger RNA (mRNA) expression of candidate immune genes in skin biopsy specimens from 85 individuals with leprosy. TOLLIP mRNA was highly and specifically correlated with IL-1R antagonist (IL-1Ra). In a case-control gene-association study with 477 cases and 1021 controls in Nepal, TOLLIP single-nucleotide polymorphism rs3793964 TT genotype was associated with increased susceptibility to leprosy (recessive, P = 1.4 × 10(-3)) and with increased skin expression of TOLLIP and IL-1Ra. Stimulation of TOLLIP-deficient monocytes with M. leprae produced significantly less IL-1Ra (P < .001), compared with control. These data suggest that M. leprae upregulates IL-1Ra by a TOLLIP-dependent mechanism. Inhibition of TOLLIP may decrease an individual's susceptibility to leprosy and offer a novel therapeutic target for IL-1-dependent diseases.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aea1141537ddd4e5c14030016c9d14bdTest
https://pubmed.ncbi.nlm.nih.gov/26610735Test -
6
المصدر: Microbiology and Molecular Biology Reviews. 74:589-620
مصطلحات موضوعية: Genetics, Tuberculosis, biology, Genetic Linkage, Genome, Human, Reviews, Genome-wide association study, Disease, biology.organism_classification, medicine.disease, Microbiology, Phenotype, Mycobacterium leprae, Infectious Diseases, Immune system, Immunity, Leprosy, Immunology, medicine, Humans, Genetic Predisposition to Disease, Molecular Biology, Genome-Wide Association Study
الوصف: SUMMARY Despite the availability of effective treatment for several decades, leprosy remains an important medical problem in many regions of the world. Infection with Mycobacterium leprae can produce paucibacillary disease, characterized by well-formed granulomas and a Th1 T-cell response, or multibacillary disease, characterized by poorly organized cellular infiltrates and Th2 cytokines. These diametric immune responses confer states of relative resistance or susceptibility to leprosy, respectively, and have well-defined clinical manifestations. As a result, leprosy provides a unique opportunity to dissect the genetic basis of human in vivo immunity. A series of studies over the past 40 years suggests that host genes influence the risk of leprosy acquisition and the predilection for different clinical forms of the disease. However, a comprehensive, cellular, and molecular view of the genes and variants involved is still being assembled. In this article, we review several decades of human genetic studies of leprosy, including a number of recent investigations. We emphasize genetic analyses that are validated by the replication of the same phenotype in independent studies or supported by functional experiments demonstrating biological mechanisms of action for specific polymorphisms. Identifying and functionally exploring the genetic and immunological factors that underlie human susceptibility to leprosy have yielded important insights into M. leprae pathogenesis and are likely to advance our understanding of the immune response to other pathogenic mycobacteria. This knowledge may inform new treatment or vaccine strategies for leprosy or tuberculosis.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76bed8aee177a3d10713549fbadb1b3dTest
https://doi.org/10.1128/mmbr.00025-10Test -
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المؤلفون: Ulrike B. Carlino, Thomas A. Russo, James R. Johnson, James C. Vary, Adam L. Stell, Phillip I. Tarr, Sima S. Bilge
المصدر: Infection and Immunity. 68:3040-3047
مصطلحات موضوعية: Antiinfective agent, biology, Molecular epidemiology, Immunology, Virulence, medicine.disease_cause, biology.organism_classification, Microbiology, Enterobacteriaceae, Infectious Diseases, Phylogenetics, medicine, Parasitology, Gene, Escherichia coli, Bacteria
الوصف: Two novel putative Escherichia coli virulence genes, iha and iroN from E. coli ( iroN E. coli ), were detected in 55 and 39%, respectively, of 67 E. coli isolates from patients with urosepsis. iha and iroN E. coli exhibited divergent associations with other putative virulence genes, phylogenetic markers, host characteristics, and antimicrobial resistance.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::d1d05d878c4f3fe35a86829a2e729f07Test
https://doi.org/10.1128/iai.68.5.3040-3047.2000Test -
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المؤلفون: James C. Vary, Sima S. Bilge, Leanne Mills, Patricia M. Desmarchelier, Phillip I. Tarr, Narelle Fegan
المصدر: ResearcherID
مصطلحات موضوعية: Microbiology (medical), Enzyme-Linked Immunosorbent Assay, Escherichia coli O157, medicine.disease_cause, Polymerase Chain Reaction, Sensitivity and Specificity, law.invention, Microbiology, chemistry.chemical_compound, fluids and secretions, law, medicine, Animals, Humans, Serotyping, Escherichia coli, Transaminases, Polymerase chain reaction, Immunoassay, biology, medicine.diagnostic_test, O Antigens, Bacteriology, Raw milk, biology.organism_classification, DNA extraction, Enterobacteriaceae, Molecular biology, Bacterial Typing Techniques, Milk, chemistry, Carbohydrate Epimerases, DNA, Bacteria
الوصف: A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected E. coli O157 per ml in raw milk following enrichment.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff55b1bec77a913029bef8940f303c72Test
https://doi.org/10.1128/jcm.36.6.1801-1804.1998Test -
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المؤلفون: Phillip I. Tarr, Sima S. Bilge, James C. Vary, Scott F. Dowell
المصدر: Infection and Immunity. 64:4795-4801
مصطلحات موضوعية: Molecular Sequence Data, Immunology, Mutant, Biology, Escherichia coli O157, medicine.disease_cause, Microbiology, Bacterial Adhesion, Plasmid, medicine, Humans, Amino Acid Sequence, Cloning, Molecular, Escherichia coli, Transaminases, Base Sequence, Genetic Complementation Test, Nucleic acid sequence, O Antigens, biology.organism_classification, Molecular biology, Enterobacteriaceae, Complementation, Mutagenesis, Insertional, Open reading frame, Infectious Diseases, Genes, Bacterial, Vibrio cholerae, DNA Transposable Elements, Parasitology, Carbohydrate Epimerases, Research Article, Bacterial Outer Membrane Proteins, HeLa Cells
الوصف: Shiga-toxigenic Escherichia coli strains belonging to serotype O157 are important human pathogens, but the genetic basis of expression of the O157 antigen and the role played by the lipopolysaccharide O side chain in the adherence of this organism to epithelial cells are not understood. We performed TnphoA mutagenesis on E. coli O157:H7 strain 86-24 to identify a mutant (strain F12) deficient in O-antigen expression. Nucleotide sequence analysis demonstrated that the transposon inserted within an open reading frame with significant homology to rfbE of Vibrio cholerae O1 (U. H. Stroeher, L. E. Karageorgos, R. Morona, and P. A. Manning, Proc. Natl. Acad. Sci. USA 89:2566-2570, 1992), which is postulated to encode perosamine synthetase. This open reading frame was designated rfbE(EcO157:H7). The guanine-plus-cytosine fraction (0.35) suggests that rfbE(EcO157:H7) may have originated in a species other than E. coli. rfbE(EcO157:H7) is conserved in nontoxigenic E. coli O157 strains expressing a variety of other flagellar antigens but is not found in E. coli O55:H7 strains, which are more closely related to E. coli O157:H7. Strain F12 was significantly more adherent to HeLa cells in a quantitative adherence assay than was its E. coli O157:H7 parent, but they did not differ in other phenotypes. Restoration of the expression of the O side chain by complementation of the TnphoA mutation in strain F12 by a plasmid expressing intact rfbE(EcO157:H7) reduced the adherence of the hyperadherent strain F12. We conclude that rfbE(EcO157:H7) is necessary for the expression of the O157 antigen, that acquisition of E. coli rfb genes occurred independently in E. coli O157:H7 and unrelated O157 strains, and that the O side chain of E. coli O157:H7 lipopolysaccharide interferes with the adherence of E. coli O157:H7 to epithelial cells.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f25b7d428bb04cb3b196545eafe2262dTest
https://doi.org/10.1128/iai.64.11.4795-4801.1996Test -
10
المؤلفون: Glenna J. Peterson, Richard D. Wells, Susan J. F. van den Eeden, Chhatra B. Kunwar, James C. Vary, William R. Berrington, Thomas R. Hawn, Annemieke Geluk, Kapil D. Neupane, Deanna A. Hagge
المصدر: PLoS Neglected Tropical Diseases, Vol 8, Iss 11, p e3263 (2014)
PLoS Neglected Tropical Diseases, 8(11)
PLoS Neglected Tropical Diseasesمصطلحات موضوعية: Male, Bacterial Diseases, Chemokine, Pathology, Erythema Nodosum, 0302 clinical medicine, Medicine and Health Sciences, Cluster Analysis, CCL17, Mycobacterium leprae, Skin, Innate Immune System, 0303 health sciences, Lepromatous leprosy, integumentary system, biology, medicine.diagnostic_test, lcsh:Public aspects of medicine, Middle Aged, Leprosy, Tuberculoid, Interleukin-10, 3. Good health, Leprosy, Lepromatous, Infectious Diseases, Chemokines, CC, Female, Leprosy, Research Article, Neglected Tropical Diseases, Adult, medicine.medical_specialty, lcsh:Arctic medicine. Tropical medicine, lcsh:RC955-962, Immunology, Microbiology, Young Adult, 03 medical and health sciences, Biopsy, medicine, Humans, 030304 developmental biology, business.industry, Macrophages, Immunity, Public Health, Environmental and Occupational Health, CCL18, Biology and Life Sciences, lcsh:RA1-1270, Gene Expression Regulation, Bacterial, Tropical Diseases, medicine.disease, biology.organism_classification, Immune System, biology.protein, Chemokine CCL17, business, Biomarkers, 030215 immunology
الوصف: Background Leprosy is characterized by polar clinical, histologic and immunological presentations. Previous immunologic studies of leprosy polarity were limited by the repertoire of cytokines known at the time. Methodology We used a candidate gene approach to measure mRNA levels in skin biopsies from leprosy lesions. mRNA from 24 chemokines and cytokines, and 6 immune cell type markers were measured from 85 Nepalese leprosy subjects. Selected findings were confirmed with immunohistochemistry. Principal Results Expression of three soluble mediators (CCL18, CCL17 and IL-10) and one macrophage cell type marker (CD14) was significantly elevated in lepromatous (CCL18, IL-10 and CD14) or tuberculoid (CCL17) lesions. Higher CCL18 protein expression by immunohistochemistry and a trend in increased serum CCL18 in lepromatous lesions was observed. No cytokines were associated with erythema nodosum leprosum or Type I reversal reaction following multiple comparison correction. Hierarchical clustering suggested that CCL18 was correlated with cell markers CD209 and CD14, while neither CCL17 nor CCL18 were highly correlated with classical TH1 and TH2 cytokines. Conclusions Our findings suggest that CCL17 and CCL18 dermal expression is associated with leprosy polarity.
Author Summary Leprosy presents with a polarized spectrum, with lepromatous leprosy having high bacillary numbers and TH2 dermal cytokines, versus tuberculoid leprosy showing very few bacilli and TH1 cytokines. The mechanism underlying this polarized presentation is largely unknown. In the following study, we isolated mRNA from skin biopsies from 85 individuals with leprosy and measured the expression of a panel of 24 cytokines and 6 cell markers. We found that three soluble mediators (CCL17, CCL18 and IL10) and one cell marker (CD14) were differentially expressed in leprosy dermal lesions. CCL18 and IL10 were more highly expressed within lepromatous lesions, and CCL17 and CD14 were more highly expressed within tuberculoid lesions. In addition, CCL18 protein expression was confirmed by immunostaining. CCL17 and CCL18, were more strongly associated with leprosy polarity than traditional TH1 and TH2 cytokines. These data suggest that newer soluble chemokines may be important in leprosy pathogenesis and uncover a molecular signature of the two polar phenotypes of leprosy, which may be useful in future diagnostics.الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b849469a4acf315987235f6da2e409cTest
https://doi.org/10.1371/journal.pntd.0003263Test
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