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1دورية أكاديمية
المؤلفون: Kaori Nozawa, Yoshitaka Fujihara, Darius J. Devlin, Ricardo E. Deras, Katarzyna Kent, Irina V. Larina, Kohei Umezu, Zhifeng Yu, Courtney M. Sutton, Qiuji Ye, Laura K. Dean, Chihiro Emori, Masahito Ikawa, Thomas X. Garcia, Martin M. Matzuk
المصدر: BMC Biology, Vol 20, Iss 1, Pp 1-19 (2022)
مصطلحات موضوعية: Sperm defects, ERAD, Male infertility, Contraceptive, PROTACs, Biology (General), QH301-705.5
الوصف: Abstract Background Ubiquitination is a post-translational modification required for a number of physiological functions regulating protein homeostasis, such as protein degradation. The endoplasmic reticulum (ER) quality control system recognizes and degrades proteins no longer needed in the ER through the ubiquitin–proteasome pathway. E2 and E3 enzymes containing a transmembrane domain have been shown to function in ER quality control. The ER transmembrane protein UBE2J1 is a E2 ubiquitin-conjugating enzyme reported to be essential for spermiogenesis at the elongating spermatid stage. Spermatids from Ube2j1 KO male mice are believed to have defects in the dislocation step of ER quality control. However, associated E3 ubiquitin-protein ligases that function during spermatogenesis remain unknown. Results We identified four evolutionarily conserved testis-specific E3 ubiquitin-protein ligases [RING finger protein 133 (Rnf133); RING finger protein 148 (Rnf148); RING finger protein 151 (Rnf151); and Zinc finger SWIM-type containing 2 (Zswim2)]. Using the CRISPR/Cas9 system, we generated and analyzed the fertility of mutant mice with null alleles for each of these E3-encoding genes, as well as double and triple knockout (KO) mice. Male fertility, male reproductive organ, and sperm-associated parameters were analyzed in detail. Fecundity remained largely unaffected in Rnf148, Rnf151, and Zswim2 KO males; however, Rnf133 KO males displayed severe subfertility. Additionally, Rnf133 KO sperm exhibited abnormal morphology and reduced motility. Ultrastructural analysis demonstrated that cytoplasmic droplets were retained in Rnf133 KO spermatozoa. Although Rnf133 and Rnf148 encode paralogous genes that are chromosomally linked and encode putative ER transmembrane E3 ubiquitin-protein ligases based on their protein structures, there was limited functional redundancy of these proteins. In addition, we identified UBE2J1 as an E2 ubiquitin-conjugating protein that interacts with RNF133. Conclusions Our studies reveal that RNF133 is a testis-expressed E3 ubiquitin-protein ligase that plays a critical role for sperm function during spermiogenesis. Based on the presence of a transmembrane domain in RNF133 and its interaction with the ER containing E2 protein UBE2J1, we hypothesize that these ubiquitin-regulatory proteins function together in ER quality control during spermatogenesis.
وصف الملف: electronic resource
العلاقة: https://doaj.org/toc/1741-7007Test
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2دورية أكاديمية
المؤلفون: Deirdre M. Scully, Irina V. Larina
المصدر: Frontiers in Cell and Developmental Biology, Vol 10 (2022)
مصطلحات موضوعية: mouse, embryo, development, optical coherence tomography, in vivo imaging, heart, Biology (General), QH301-705.5
الوصف: With the explosion of gene editing tools in recent years, there has been a much greater demand for mouse embryo phenotyping, and traditional methods such as histology and histochemistry experienced a methodological renaissance as they became the principal tools for phenotyping. However, it is important to explore alternative phenotyping options to maximize time and resources and implement volumetric structural analysis for enhanced investigation of phenotypes. Cardiovascular phenotyping, in particular, is important to perform in vivo due to the dramatic structural and functional changes that occur in heart development over relatively short periods of time. Optical coherence tomography (OCT) is one of the most exciting advanced imaging techniques emerging within the field of developmental biology, and this review provides a summary of how it is currently being implemented in mouse embryo investigations and phenotyping. This review aims to provide an understanding of the approaches used in optical coherence tomography and how they can be applied in embryology and developmental biology, with the overall aim of bridging the gap between biology and technology.
وصف الملف: electronic resource
العلاقة: https://www.frontiersin.org/articles/10.3389/fcell.2022.1000237/fullTest; https://doaj.org/toc/2296-634XTest
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3دورية أكاديمية
المؤلفون: Shang Wang, Irina V. Larina
المصدر: Journal of Cardiovascular Development and Disease, Vol 9, Iss 8, p 267 (2022)
مصطلحات موضوعية: embryonic heart, valveless pumping, four-dimensional imaging, functional imaging, cardiodynamics, hemodynamics, Diseases of the circulatory (Cardiovascular) system, RC666-701
الوصف: In vertebrates, the coordinated beat of the early heart tube drives cardiogenesis and supports embryonic growth. How the heart pumps at this valveless stage marks a fascinating problem that is of vital significance for understanding cardiac development and defects. The developing heart achieves its function at the same time as continuous and dramatic morphological changes, which in turn modify its pumping dynamics. The beauty of this muti-time-scale process also highlights its complexity that requires interdisciplinary approaches to study. High-resolution optical imaging, particularly fast, four-dimensional (4D) imaging, plays a critical role in revealing the process of pumping, instructing numerical modeling, and enabling biomechanical analyses. In this review, we aim to connect the investigation of valveless pumping mechanisms with the recent advancements in embryonic cardiodynamic imaging, facilitating interactions between these two areas of study, in hopes of encouraging and motivating innovative work to further understand the early heartbeat.
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: Shang Wang, Irina V. Larina
المصدر: Cell Reports, Vol 36, Iss 2, Pp 109382- (2021)
مصطلحات موضوعية: oviduct, oocyte, preimplantation embryo, optical coherence tomography, intravital window, volumetric imaging, Biology (General), QH301-705.5
الوصف: Summary: Developmental biologists have always relied on imaging to shed light on dynamic cellular events. However, processes such as mammalian fertilization and embryogenesis are generally inaccessible for direct imaging. In consequence, how the oviduct (fallopian tube) facilitates the transport of gametes and preimplantation embryos continues to be unanswered. Here we present a combination of intravital window and optical coherence tomography for dynamic, volumetric, in vivo imaging of oocytes and embryos as they are transported through the mouse oviduct. We observed location-dependent circling, oscillating, and long-distance bi-directional movements of oocytes and embryos that suggest regulatory mechanisms driving transport and question established views in the field. This in vivo imaging approach can be combined with a variety of genetic and pharmacological manipulations for live functional analysis, bringing the potential to investigate reproductive physiology in its native state.
وصف الملف: electronic resource
العلاقة: http://www.sciencedirect.com/science/article/pii/S2211124721007804Test; https://doaj.org/toc/2211-1247Test
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5دورية أكاديمية
المؤلفون: Anthony P. Barrasso, Shang Wang, Xuefei Tong, Audrey E. Christiansen, Irina V. Larina, Ross A. Poché
المصدر: Neural Development, Vol 13, Iss 1, Pp 1-14 (2018)
مصطلحات موضوعية: Live imaging, Mouse retinal progenitor cells, Interkinetic nuclear migration, Cyclin D1, Horizontal neurons, Neurology. Diseases of the nervous system, RC346-429
الوصف: Abstract Background Ex vivo, whole-mount explant culture of the rodent retina has proved to be a valuable approach for studying retinal development. In a limited number of recent studies, this method has been coupled to live fluorescent microscopy with the goal of directly observing dynamic cellular events. However, retinal tissue thickness imposes significant technical limitations. To obtain 3-dimensional images with high quality axial resolution, investigators are restricted to specific areas of the retina and require microscopes, such as 2-photon, with a higher level of depth penetrance. Here, we report a retinal live imaging method that is more amenable to a wider array of imaging systems and does not compromise resolution of retinal cross-sectional area. Results Mouse retinal slice cultures were prepared and standard, inverted confocal microscopy was used to generate movies with high quality resolution of retinal cross-sections. To illustrate the ability of this method to capture discrete, physiologically relevant events during retinal development, we imaged the dynamics of the Fucci cell cycle reporter in both wild type and Cyclin D1 mutant retinal progenitor cells (RPCs) undergoing interkinetic nuclear migration (INM). Like previously reported for the zebrafish, mouse RPCs in G1 phase migrated stochastically and exhibited overall basal drift during development. In contrast, mouse RPCs in G2 phase displayed directed, apical migration toward the ventricular zone prior to mitosis. We also determined that Cyclin D1 knockout RPCs in G2 exhibited a slower apical velocity as compared to wild type. These data are consistent with previous IdU/BrdU window labeling experiments on Cyclin D1 knockout RPCs indicating an elongated cell cycle. Finally, to illustrate the ability to monitor retinal neuron differentiation, we imaged early postnatal horizontal cells (HCs). Time lapse movies uncovered specific HC neurite dynamics consistent with previously published data showing an instructive role for transient vertical neurites in HC mosaic formation. Conclusions We have detailed a straightforward method to image mouse retinal slice culture preparations that, due to its relative ease, extends live retinal imaging capabilities to a more diverse group of scientists. We have also shown that, by using a slice technique, we can achieve excellent lateral resolution, which is advantageous for capturing intracellular dynamics and overall cell movements during retinal development and differentiation.
وصف الملف: electronic resource
العلاقة: http://link.springer.com/article/10.1186/s13064-018-0120-yTest; https://doaj.org/toc/1749-8104Test
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6دورية أكاديمية
المؤلفون: Andrew L. Lopez, Shang Wang, Irina V. Larina
المصدر: Journal of Cardiovascular Development and Disease, Vol 7, Iss 4, p 42 (2020)
مصطلحات موضوعية: mouse, cardiogenesis, in vivo imaging, optical coherence tomography, embryo culture, cardiodynamics, Diseases of the circulatory (Cardiovascular) system, RC666-701
الوصف: The embryonic heart is an active and developing organ. Genetic studies in mouse models have generated great insight into normal heart development and congenital heart defects, and suggest mechanical forces such as heart contraction and blood flow to be implicated in cardiogenesis and disease. To explore this relationship and investigate the interplay between biomechanical forces and cardiac development, live dynamic cardiac imaging is essential. Cardiodynamic imaging with optical coherence tomography (OCT) is proving to be a unique approach to functional analysis of the embryonic mouse heart. Its compatibility with live culture systems, reagent-free contrast, cellular level resolution, and millimeter scale imaging depth make it capable of imaging the heart volumetrically and providing spatially resolved information on heart wall dynamics and blood flow. Here, we review the progress made in mouse embryonic cardiodynamic imaging with OCT, highlighting leaps in technology to overcome limitations in resolution and acquisition speed. We describe state-of-the-art functional OCT methods such as Doppler OCT and OCT angiography for blood flow imaging and quantification in the beating heart. As OCT is a continuously developing technology, we provide insight into the future developments of this area, toward the investigation of normal cardiogenesis and congenital heart defects.
وصف الملف: electronic resource
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7دورية أكاديمية
المؤلفون: Narendran Sudheendran, Maleeha Mashiatulla, Raksha Raghunathan, Saba H. Syed, Mary E. Dickinson, Irina V. Larina, Kirill V. Larin
المصدر: Journal of Biomedical Photonics & Engineering, Vol 1, Iss 1, Pp 90-95 (2015)
مصطلحات موضوعية: Eye volume, in utero, mouse embryo, optical coherence tomography, Applied optics. Photonics, TA1501-1820, Medical technology, R855-855.5
الوصف: Mouse models are commonly used as research tools to understand regulatory pathways affected by human diseases and disorders. Live imaging tools for visualization of mouse embryonic ocular tissues would be beneficial in research associated with developmental ocular defects. In this study, in utero quantitative assessment of ocular structures in mouse embryos was performed with a swept-source optical coherence tomography (SSOCT). To define developmental changes in eye morphology in live embryos, the volume of the embryonic eye lens and the globe at different embryonic stages ranging from E13.5 to E18.5 was quantified. It is determined that the major axis diameter of the eye lens and the globe was found to increase from 0.44±0.18 mm to 0.98±0.05 mm and from 0.56±0.22 mm to 1.23±0.14 mm, respectively, as the embryo ages from E13.5 to E18.5. For the same stages, the volume of the eye lens and globe was found to increase from 0.028±0.027 mm3 to 0.32±0.08 mm3 and from 0.085±0.08 mm3 to 0.75±0.27 mm3, respectively. These results suggest that OCT can accurately assess developmental processes of ocular structures and can be potentially used to assess embryonic ocular growth in mouse mutants with eye abnormalities and to study the effect of toxicological and pharmacological agents.
وصف الملف: electronic resource
العلاقة: http://jbpe.ssau.ru/index.php/JBPE/article/view/2039Test; https://doaj.org/toc/2411-2844Test
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8دورية أكاديمية
المؤلفون: Чувелева Ирина Владимировна, Irina V. Chuveleva, Садыкова Марина Юрьевна, Marina I. Sadykova, Ларина Ирина Васильевна, Irina V. Larina
المصدر: Pedagogical excellence and pedagogical technologies; 176-177 ; Педагогическое мастерство и современные педагогические технологии; 176-177
مصطلحات موضوعية: воспитатель, наставничество, учебные заведения
الوصف: Статья посвящена вопросу о системе наставничества в работе со студентами педагогических учебных заведений.
وصف الملف: text/html
العلاقة: info:eu-repo/semantics/altIdentifier/isbn/978-5-6049492-1-4; https://interactive-plus.ru/e-articles/850/Action850-559208.pdfTest; https://interactive-plus.ru/files/Books/850/Cover-850.jpg?req=559208Test; https://interactive-plus.ru/article/559208/discussion_platformTest
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9دورية أكاديمية
المؤلفون: Ларина Ирина Васильевна, Irina V. Larina, Садыкова Марина Юрьевна, Marina I. Sadykova, Чувелева Ирина Владимировна, Irina V. Chuveleva
المصدر: Education and science in the modern context; 68-70 ; Образование и наука в современных реалиях; 68-70
مصطلحات موضوعية: подвижные игры, дошкольники с ОВЗ
الوصف: Статья посвящена вопросу привлечения дошкольников с ОВЗ к спорту посредством народных игр.
وصف الملف: text/html
العلاقة: info:eu-repo/semantics/altIdentifier/isbn/978-5-6047675-4-2; https://interactive-plus.ru/e-articles/802/Action802-556081.pdfTest; https://interactive-plus.ru/files/Books/625fc8640c63c.jpg?req=556081Test; https://interactive-plus.ru/article/556081/discussion_platformTest
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10
المؤلفون: Shang Wang, Irina V Larina
المصدر: Reproduction
مصطلحات موضوعية: Diagnostic Imaging, Embryology, Reproduction, Obstetrics and Gynecology, Oviducts, Cell Biology, Article, Mice, Germ Cells, Endocrinology, Reproductive Medicine, Pregnancy, Humans, Animals, Female, Fallopian Tubes
الوصف: In brief In vivo imaging of gametes and embryos in the oviduct enables new studies of the native processes that lead to fertilization and pregnancy. This review article discusses recent advancements in the in vivo imaging methods and insights which contribute to understanding the oviductal function. Abstract Understanding the physiological dynamics of gametes and embryos in the fallopian tube (oviduct) has significant implications for managing reproductive disorders and improving assisted reproductive technologies. Recent advancements in imaging of the mouse oviduct in vivo uncovered fascinating dynamics of gametes and embryos in their native states. These new imaging approaches and observations are bringing exciting momentum to uncover the otherwise-hidden processes orchestrating fertilization and pregnancy. For mechanistic investigations, in vivo imaging in genetic mouse models enables dynamic phenotyping of gene functions in the reproductive process. Here, we review these imaging methods, discuss insights recently revealed by in vivo imaging, and comment on emerging directions, aiming to stimulate new in vivo studies of reproductive dynamics.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ca3bb8fdffb165271c94315c47a0f0eTest
https://doi.org/10.1530/rep-22-0250Test
النص الكامل 
