المؤلفون: Manuela De Michele, Paola Piscopo, Matteo Costanzo, Svetlana Lorenzano, Alessio Crestini, Roberto Rivabene, Valeria Manzini, Luca Petraglia, Marta Iacobucci, Irene Berto, Oscar Gaetano Schiavo, Antonella Conte, Daniele Belvisi, Alfredo Berardelli, Danilo Toni

المصدر: Biomedicines, Vol 12, Iss 3, p 670 (2024)

مصطلحات موضوعية: neurogenesis, stroke, humans, rTMS, miRNA, Netrin-1, Biology (General), QH301-705.5

الوصف: Background: Ischemic stroke may trigger neuroplastic changes via proliferation, migration towards the lesion, and differentiation of neuroprogenitor cells into mature neurons. Repetitive Transcranial Magnetic Stimulation (rTMS) may promote brain plasticity. This study aimed to assess rTMS’s effect on post-stroke endogenous neuroplasticity by dosing plasma miRs 17~92, Netrin-1, Sema3A, and BDNF. Methods: In this case-controlled study, we randomized 19 ischemic stroke patients within five days from symptoms onset (T0) to neuronavigated-rTMS or sham stimulation. Stimulation was applied on the stroke hemisphere daily between the 7th and 14th day from stroke onset. Blood samples were collected at T0, before the first rTMS section (T7), and at the end of the last rTMS session (T14). Five healthy controls were also enrolled in this study. Results: Of 19 patients, 10 received rTMS and 9 sham stimulation. Compared with the sham group, in the rTMS group, plasma levels of miRs17~92 and Ntn-1 significantly increased whereas Sema3A levels tended to decrease. In multivariate linear regression analyses, rTMS was independently related to Ntn-1 and miR-25 levels at T14. Conclusions: We found an association between rTMS and neurogenesis/axonogenesis biomarker enhancement. Our preliminary data suggest that rTMS may positively interfere with natural endogenous plasticity phenomena of the post-ischemic human brain.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2227-9059/12/3/670Test; https://doaj.org/toc/2227-9059Test

الوصول الحر: https://doaj.org/article/5fd42eba3b414fd88520a777df7af5f7Test

المؤلفون: Manuela De Michele, Giulia d’Amati, Martina Leopizzi, Marta Iacobucci, Irene Berto, Svetlana Lorenzano, Laura Mazzuti, Ombretta Turriziani, Oscar G. Schiavo, Danilo Toni

المصدر: Journal of Hematology & Oncology, Vol 15, Iss 1, Pp 1-5 (2022)

مصطلحات موضوعية: Diseases of the blood and blood-forming organs, RC633-647.5, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282

الوصف: Abstract The pathophysiology of COVID-19-associated coagulopathy is complex and not fully understood. SARS-CoV-2 spike protein (SP) may activate platelets and interact with fibrin(ogen). We aimed to investigate whether isolated SP can be present in clots retrieved in COVID-19 patients with acute ischemic stroke (by mechanical thrombectomy) and myocardial infarction. In this pilot study, we could detect SP, but not nucleocapsid protein, on platelets of COVID-19 patients’ thrombi. In addition, in all three COVID-19 thrombi analyzed for molecular biology, no SARS-CoV-2 RNA could be detected by real-time polymerase chain reaction. These data could support the hypothesis that free SP, besides the whole virus, may be the trigger of platelet activation and clot formation in COVID-19.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1756-8722Test

الوصول الحر: https://doaj.org/article/d3b139e7e09f418a9606e7bef2b53ac0Test

المؤلفون: Manuela De Michele, Svetlana Lorenzano, Paola Piscopo, Roberto Rivabene, Alessio Crestini, Antonio Chistolini, Lucia Stefanini, Fabio M. Pulcinelli, Irene Berto, Roberta Campagna, Paolo Amisano, Marta Iacobucci, Carlo Cirelli, Anne Falcou, Ettore Nicolini, Oscar G. Schiavo, Danilo Toni

المصدر: Frontiers in Cardiovascular Medicine, Vol 9 (2023)

مصطلحات موضوعية: stroke, SARS-CoV-2, COVID-19, coagulation, thrombosis, platelet activation, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Background and purposeAcute ischemic stroke (AIS) is a fearful complication of Coronavirus Disease-2019 (COVID-19). Aims of this study were to compare clinical/radiological characteristics, endothelial and coagulation dysfunction between acute ischemic stroke (AIS) patients with and without COVID-19 and to investigate if and how the SARS-CoV-2 spike protein (SP) was implicated in triggering platelet activation.MethodsWe enrolled AIS patients with COVID-19 within 12 h from onset and compared them with an age- and sex-matched cohort of AIS controls without COVID-19. Neuroimaging studies were performed within 24 h. Blood samples were collected in a subset of 10 patients.ResultsOf 39 AIS patients, 22 had COVID-19 and 17 did not. Admission levels of Factor VIII and von Willebrand factor antigen were significantly higher in COVID-19 patients and positively correlated with the infarct volume. In multivariate linear regression analyses, COVID-19 was an independent predictor of infarct volume (B 20.318, Beta 0.576, 95%CI 6.077–34.559; p = 0.011). SP was found in serum of 2 of the 10 examined COVID-19 patients. Platelets from healthy donors showed a similar degree of procoagulant activation induced by COVID-19 and non-COVID-19 patients' sera. The anti-SP and anti-FcγRIIA blocking antibodies had no effect in modulating platelet activity in both groups.ConclusionsSARS-CoV-2 infection seems to play a major role in endothelium activation and infarct volume extension during AIS.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fcvm.2022.1097229/fullTest; https://doaj.org/toc/2297-055XTest

الوصول الحر: https://doaj.org/article/d93256c7b1ef4ffbb191bcbc6c0cf006Test

المؤلفون: Manuela De Michele, Paola Piscopo, Alessio Crestini, Roberto Rivabene, Giulia d’Amati, Martina Leopizzi, Lucia Stefanini, Fabio Pulcinelli, Antonio Chistolini, Marta Iacobucci, Oscar G. Schiavo, Irene Berto, Ettore Nicolini, Luca Petraglia, Maria Teresa Di Mascio, Danilo Toni

المصدر: Haematologica, Vol 107, Iss 7 (2022)

مصطلحات موضوعية: Diseases of the blood and blood-forming organs, RC633-647.5

وصف الملف: electronic resource

العلاقة: https://haematologica.org/article/view/10541Test; https://doaj.org/toc/0390-6078Test; https://doaj.org/toc/1592-8721Test

الوصول الحر: https://doaj.org/article/687d7e115cc9492c84c34c8dc6101448Test

المؤلفون: Manuela De Michele, Joshua Kahan, Irene Berto, Oscar G. Schiavo, Marta Iacobucci, Danilo Toni, Alexander E. Merkler

المساهمون: De Michele, Manuela, Kahan, Joshua, Berto, Irene, Schiavo, Oscar G., Iacobucci, Marta, Toni, Danilo, Merkler, Alexander E.

مصطلحات موضوعية: COVID, CoV, SARS, pandemic, stroke, vaccine, COVID-19 vaccine, human, platelet factor 4, SARS-CoV-2, vaccination, COVID-19, thrombocytopenia, thrombosi, vaccines

الوقت: 19, 2

الوصف: The risk of stroke and cerebrovascular disease complicating infection with SARS-CoV-2 has been extensively reported since the onset of the pandemic. The striking efforts of many scientists in cooperation with regulators and governments worldwide have rapidly brought the development of a large landscape of vaccines against SARS-CoV-2. The novel DNA and mRNA vaccines have offered great flexibility in terms of antigen production and led to an unprecedented rapidity in effective and safe vaccine production. However, as mass vaccination has progressed, rare but catastrophic cases of thrombosis have occurred in association with thrombocytopenia and antibodies against PF4 (platelet factor 4). This catastrophic syndrome has been named vaccine-induced immune thrombotic thrombocytopenia. Rarely, ischemic stroke can be the symptom onset of vaccine-induced immune thrombotic thrombocytopenia or can complicate the course of the disease. In this review, we provide an overview of stroke and cerebrovascular disease as a complication of the SARS-CoV-2 infection and outline the main clinical and radiological characteristics of cerebrovascular complications of vaccinations, with a focus on vaccine-induced immune thrombotic thrombocytopenia. Based on the available data from the literature and from our experience, we propose a therapeutic protocol to manage this challenging condition. Finally, we highlight the overlapping pathophysiologic mechanisms of SARS-CoV-2 infection and vaccination leading to thrombosis.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/35420916; info:eu-repo/semantics/altIdentifier/wos/WOS:000781277900007; volume:130; issue:8; firstpage:1187; lastpage:1203; numberofpages:17; journal:CIRCULATION RESEARCH; http://hdl.handle.net/11573/1629671Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85128304058

الإتاحة: https://doi.org/10.1161/circresaha.122.319954Test
http://hdl.handle.net/11573/1629671Test

المؤلفون: Manuela DE MICHELE, Irene BERTO, Luca PETRAGLIA, Oscar G. SCHIAVO, Federica MORET, Ettore NICOLINI, Nicoletta CARACCIOLO, Maria Teresa DI MASCIO, Danilo TONI

المساهمون: DE MICHELE, Manuela, Berto, Irene, Petraglia, Luca, Schiavo, Oscar G., Moret, Federica, Nicolini, Ettore, Caracciolo, Nicoletta, Teresa DI MASCIO, Maria, Toni, Danilo

العلاقة: journal:ITALIAN JOURNAL OF EMERGENCY MEDICINE; http://hdl.handle.net/11577/3406658Test

الإتاحة: https://doi.org/10.23736/S2532-1285.21.00087-2Test
http://hdl.handle.net/11577/3406658Test

المؤلفون: Ettore Nicolini, Manuela De Michele, Anne Falcou, Luca Petraglia, Irene Berto, Danilo Toni

المساهمون: Nicolini, Ettore, De Michele, Manuela, Falcou, Anne, Petraglia, Luca, Berto, Irene, Toni, Danilo

مصطلحات موضوعية: "Thrombus" "Stroke" "Histology" "Mechanical thrombectomy"

وصف الملف: ELETTRONICO

العلاقة: numberofpages:15; journal:VESSEL PLUS; http://hdl.handle.net/11577/3406674Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85140993155; https://vpjournal.net/article/view/4228Test

الإتاحة: https://doi.org/10.20517/2574-1209.2021.78Test
http://hdl.handle.net/11577/3406674Test
https://vpjournal.net/article/view/4228Test

المؤلفون: Manuela De Michele, Marta Iacobucci, Federica Letteri, Chiara Andreoli, Svetlana Lorenzano, Rossella Canese, Luca Petraglia, Irene Berto, Oscar Gaetano Schiavo, Claudio Di Biasi, Danilo Toni

المصدر: Neurological Sciences. 44:967-978

مصطلحات موضوعية: Humans, MR spectroscopy, Neurogenesis, Stroke, Psychiatry and Mental health, Neurology (clinical), Dermatology, General Medicine

الوصف: In adult human brain, neurogenesis seems to persist throughout life and ischemic stroke was proved to stimulate this process. Using magnetic resonance spectroscopy (MRS), a 1.28-ppm peak, putative biomarker of neural progenitor cells (NPCs), was identified both in vitro and in vivo, i.e., in normal rat and healthy human brain. The aim of our study was to identify a 1.28-ppm peak in adult human ischemic brain by using 3.0 T multivoxel MRS.We studied 10 patients, six males, and four females, with a mean (± SD) age of 59.3 (± 17.3), at three different time points from ischemic stroke onset (T0: 5 days; T14: 14 ± 2 days; T30: 30 ± 2 days).In all patients except one, a 1.28-ppm peak at T14 was detected at the ischemic boundary (all p values 0.05). MRS performed on six voluntary age-matched healthy subjects did not detect any 1.28-ppm peak.The nature of this 1.28-pm peak is uncertain; however, our data support the hypothesis that it might represent a marker of NPCs in post-stroke human brain.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3bc7c996194a1f9e62d6a15d49fbf6eTest
https://doi.org/10.1007/s10072-022-06479-wTest

المؤلفون: Manuela De Michele (6506996), Svetlana Lorenzano (7551182), Paola Piscopo (9442844), Roberto Rivabene (14368299), Alessio Crestini (5933606), Antonio Chistolini (14368302), Lucia Stefanini (11994902), Fabio M. Pulcinelli (14368305), Irene Berto (13260301), Roberta Campagna (14368308), Paolo Amisano (14368311), Marta Iacobucci (13260298), Carlo Cirelli (9744462), Anne Falcou (9744465), Ettore Nicolini (14368314), Oscar G. Schiavo (13260307), Danilo Toni (5242841)

مصطلحات موضوعية: Cardiology, Cardiology (incl. Cardiovascular Diseases), Cardiorespiratory Medicine and Haematology not elsewhere classified, stroke, SARS-CoV-2, COVID-19, coagulation, thrombosis, platelet activation, endothelium activation

الوصف: Background and purpose Acute ischemic stroke (AIS) is a fearful complication of Coronavirus Disease-2019 (COVID-19). Aims of this study were to compare clinical/radiological characteristics, endothelial and coagulation dysfunction between acute ischemic stroke (AIS) patients with and without COVID-19 and to investigate if and how the SARS-CoV-2 spike protein (SP) was implicated in triggering platelet activation. Methods We enrolled AIS patients with COVID-19 within 12 h from onset and compared them with an age- and sex-matched cohort of AIS controls without COVID-19. Neuroimaging studies were performed within 24 h. Blood samples were collected in a subset of 10 patients. Results Of 39 AIS patients, 22 had COVID-19 and 17 did not. Admission levels of Factor VIII and von Willebrand factor antigen were significantly higher in COVID-19 patients and positively correlated with the infarct volume. In multivariate linear regression analyses, COVID-19 was an independent predictor of infarct volume (B 20.318, Beta 0.576, 95%CI 6.077–34.559; p = 0.011). SP was found in serum of 2 of the 10 examined COVID-19 patients. Platelets from healthy donors showed a similar degree of procoagulant activation induced by COVID-19 and non-COVID-19 patients' sera. The anti-SP and anti-FcγRIIA blocking antibodies had no effect in modulating platelet activity in both groups. Conclusions SARS-CoV-2 infection seems to play a major role in endothelium activation and infarct volume extension during AIS.

العلاقة: https://figshare.com/articles/figure/Image_2_SARS-CoV-2_infection_predicts_larger_infarct_volume_in_patients_with_acute_ischemic_stroke_PDF/21854256Test

الإتاحة: https://doi.org/10.3389/fcvm.2022.1097229.s002Test

المؤلفون: Manuela De Michele (6506996), Svetlana Lorenzano (7551182), Paola Piscopo (9442844), Roberto Rivabene (14368299), Alessio Crestini (5933606), Antonio Chistolini (14368302), Lucia Stefanini (11994902), Fabio M. Pulcinelli (14368305), Irene Berto (13260301), Roberta Campagna (14368308), Paolo Amisano (14368311), Marta Iacobucci (13260298), Carlo Cirelli (9744462), Anne Falcou (9744465), Ettore Nicolini (14368314), Oscar G. Schiavo (13260307), Danilo Toni (5242841)

مصطلحات موضوعية: Cardiology, Cardiology (incl. Cardiovascular Diseases), Cardiorespiratory Medicine and Haematology not elsewhere classified, stroke, SARS-CoV-2, COVID-19, coagulation, thrombosis, platelet activation, endothelium activation

الوصف: Background and purpose Acute ischemic stroke (AIS) is a fearful complication of Coronavirus Disease-2019 (COVID-19). Aims of this study were to compare clinical/radiological characteristics, endothelial and coagulation dysfunction between acute ischemic stroke (AIS) patients with and without COVID-19 and to investigate if and how the SARS-CoV-2 spike protein (SP) was implicated in triggering platelet activation. Methods We enrolled AIS patients with COVID-19 within 12 h from onset and compared them with an age- and sex-matched cohort of AIS controls without COVID-19. Neuroimaging studies were performed within 24 h. Blood samples were collected in a subset of 10 patients. Results Of 39 AIS patients, 22 had COVID-19 and 17 did not. Admission levels of Factor VIII and von Willebrand factor antigen were significantly higher in COVID-19 patients and positively correlated with the infarct volume. In multivariate linear regression analyses, COVID-19 was an independent predictor of infarct volume (B 20.318, Beta 0.576, 95%CI 6.077–34.559; p = 0.011). SP was found in serum of 2 of the 10 examined COVID-19 patients. Platelets from healthy donors showed a similar degree of procoagulant activation induced by COVID-19 and non-COVID-19 patients' sera. The anti-SP and anti-FcγRIIA blocking antibodies had no effect in modulating platelet activity in both groups. Conclusions SARS-CoV-2 infection seems to play a major role in endothelium activation and infarct volume extension during AIS.

العلاقة: https://figshare.com/articles/dataset/Table_1_SARS-CoV-2_infection_predicts_larger_infarct_volume_in_patients_with_acute_ischemic_stroke_DOCX/21854262Test

الإتاحة: https://doi.org/10.3389/fcvm.2022.1097229.s004Test