المؤلفون: Souichi Yamada, Harutaka Katano, Yuko Sato, Tadaki Suzuki, Akihiko Uda, Keita Ishijima, Motoi Suzuki, Daigo Yamada, Shizuko Harada, Hitomi Kinoshita, Phu Hoang Anh Nguyen, Hideki Ebihara, Ken Maeda, Masayuki Saijo, Shuetsu Fukushi

المصدر: Emerging Infectious Diseases, Vol 30, Iss 1, Pp 177-179 (2024)

مصطلحات موضوعية: Macacine alphaherpesvirus 1, B virus, viruses, zoonoses, Japan, Medicine, Infectious and parasitic diseases, RC109-216

الوصف: Two human patients with Macacine alphaherpesvirus 1 infection were identified in Japan in 2019. Both patients had worked at the same company, which had a macaque facility. The rhesus-genotype B virus genome was detected in cerebrospinal fluid samples from both patients.

وصف الملف: electronic resource

العلاقة: https://wwwnc.cdc.gov/eid/article/30/1/23-0435_articleTest; https://doaj.org/toc/1080-6040Test; https://doaj.org/toc/1080-6059Test

الوصول الحر: https://doaj.org/article/b323cfc58c5b47f5984a3b4fcb28ed27Test

المؤلفون: Phu Hoang Anh Nguyen, Shuetsu Fukushi, Souichi Yamada, Shizuko Harada, Tomoki Yoshikawa, Hitomi Kinoshita, Madoka Kawahara, Takuma Ogawa, Hideki Ebihara, Meng Ling Moi, Masayuki Saijo

المصدر: Microbiology Spectrum, Vol 12, Iss 1 (2024)

مصطلحات موضوعية: B virus, acyclovir, acyclovir resistant, ganciclovir, ganciclovir resistant, herpes simplex virus 1, Microbiology, QR1-502

الوصف: ABSTRACT Zoonotic infection by herpes B virus (BV) causes severe neurological disease, which in the absence of appropriate treatment can have a mortality rate of 80%. Long-term administration of acyclovir or ganciclovir is the recommended prophylactic treatment option for BV infections. However, the emergence of acyclovir- and/or ganciclovir-resistant BV may become a major problem. Here, we examined the ability of BV thymidine kinase (TK) from monkeys (mBVTK) and humans (hBVTK) to acquire resistance to acyclovir and ganciclovir. The sequences of mBVTK and hBVTK differ by two amino acids. We constructed and expressed four HSV-1-based recombinant viruses (HSV-1_mBVTK, HSV-1_hBVTK, HSV-1_VZVTK, and HSV_HSV-1TK) in which the HSV-1TK gene was deleted and the TK gene of monkey BV, human BV, varicella-zoster virus, and HSV-1 was inserted, respectively. We then tested the sensitivity of these recombinant viruses to acyclovir, ganciclovir, penciclovir, and brivudine. HSV-1_HSV-1TK and wild-type HSV-1 were more susceptible to ganciclovir, acyclovir, and penciclovir than HSV-1_VZVTK, HSV-1_hBVTK, and HSV-1_mBVTK. Both HSV-1_hBVTK and HSV-1_mBVTK exhibited similar sensitivity profiles against all tested drugs: both were susceptible to acyclovir, ganciclovir, and penciclovir but resistant to brivudine. In addition, we forced HSV-1_hBVTK to replicate under selective pressure from acyclovir or ganciclovir and then examined the ability of the BV TK gene to develop drug resistance. Investigation of the drug-resistant HSV-1_hBVTK gene revealed the absence of amino acid substitutions in BV TK, suggesting that the development of acyclovir- or ganciclovir-resistant BV through mutations in the TK gene is a rare occurrence in this experimental system. IMPORTANCE Zoonotic infection of humans with herpes B virus (BV) causes severe neurological diseases. Acyclovir (ACV) and ganciclovir (GCV), most frequently used as anti-herpes drugs, are recommended for prophylaxis and therapy in human BV infection. In this study, we examined the property of BV thymidine kinase (TK) against anti-herpes drugs using a recombinant herpes simplex virus type 1 (HSV-1) carrying BV TK gene. We found that HSV-1 carrying BV TK was similarly sensitive to GCV as HSV-1 carrying varicella zoster virus TK. In addition, we demonstrated that BV TK was not mutated in the GCV- and ACV-resistant HSV-1 carrying BV TK, suggesting that ACV- or GCV-resistant BV might be rare during treatment with these antiviral drugs. These data can provide a new insight into the properties of BV TK in terms of the development of drug resistance.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2165-0497Test

الوصول الحر: https://doaj.org/article/1ecf19b48b5c42689c7f47268b7ee312Test

المؤلفون: Sho Miyamoto, Takeshi Arashiro, Akira Ueno, Takayuki Kanno, Shinji Saito, Harutaka Katano, Shun Iida, Akira Ainai, Seiya Ozono, Takuya Hemmi, Yuichiro Hirata, Saya Moriyama, Ryutaro Kotaki, Hitomi Kinoshita, Souichi Yamada, Masaharu Shinkai, Shuetsu Fukushi, Yoshimasa Takahashi, Tadaki Suzuki

المصدر: iScience, Vol 26, Iss 2, Pp 105969- (2023)

مصطلحات موضوعية: Immunology, Immune response, Virology, Science

الوصف: Summary: The immune responses to SARS-CoV-2 variants in COVID-19 cases are influenced by various factors including pre-existing immunity via vaccination and prior infection. Elucidating the drivers for upgrading neutralizing activity to SARS-CoV-2 in COVID-19 cases with pre-existing immunity will aid in improving COVID-19 booster vaccines with enhanced cross-protection against antigenically distinct variants, including the Omicron sub-lineage BA.4/5. This study revealed that the magnitude and breadth of neutralization activity to SARS-CoV-2 variants after breakthrough infections are determined primarily by upper respiratory viral load and vaccination-infection time interval. Extensive neutralizing breadth, covering even the most antigenically distant BA.4/5, was observed in cases with higher viral load and longer time intervals. Antigenic cartography depicted a critical role of the time interval in expanding the breadth of neutralization to SARS-CoV-2 variants. Our results illustrate the importance of dosing interval optimization as well as antigen design in developing variant-proof booster vaccines.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2589004223000469Test; https://doaj.org/toc/2589-0042Test

الوصول الحر: https://doaj.org/article/89f55a1bd60340f9824840b4cd7d0c10Test

المؤلفون: Asuka Amano, Shunsuke Takahagi, Hitomi Kinoshita, Rie Nakamura, Kaori Ishii, Michihiro Hide

المصدر: Allergology International, Vol 71, Iss 3, Pp 414-416 (2022)

مصطلحات موضوعية: Immunologic diseases. Allergy, RC581-607

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1323893022000089Test; https://doaj.org/toc/1323-8930Test

الوصول الحر: https://doaj.org/article/1e1d57436ffc428ca4ebceca891f3654Test

المؤلفون: Hitomi Kinoshita, Yuzo Arima, Mika Shigematsu, Tomimasa Sunagawa, Masayuki Saijo, Kazunori Oishi, Shuji Ando

المصدر: International Journal of Infectious Diseases, Vol 105, Iss , Pp 560-566 (2021)

مصطلحات موضوعية: Scrub typhus, Japanese spotted fever, Rickettsia, Vector-borne disease, Surveillance, Japan, Infectious and parasitic diseases, RC109-216

الوصف: Objective: This study aimed to describe the epidemiological and clinical characteristics of endemics of two rickettsial diseases, scrub typhus (ST) and Japanese spotted fever (JSF), in Japan. Methods: We conducted a retrospective, descriptive epidemiological assessment of cases notified via national surveillance from 2007–2016. Results: Over the 10-year period, 4185 ST and 1765 JSF cases were notified; of these, 20 (0.48%) cases of ST and 16 (0.91%) cases of JSF were fatal at the time of reporting. The elderly had higher notification rates and fatalities. While the annual number of ST notifications was stable and cases were reported from a broad geographic range, the number of JSF reports increased three-fold, expanding from the southwest to the east. The seasonality of ST varied by region and was more common during spring/summer in the north and autumn/winter in the south; 78% of cases occurred during autumn/winter, mainly in the southern region. Most of the fatal ST cases occurred in the spring/summer and occurred in the northern region. Conclusion: Our analysis identified seasonal and regional variations in the distribution of rickettsiosis. These variations were most likely to be related to the ecology of the vectors and etiological agents. Knowing the recent epidemiological and clinical features of ST and JSF can support clinical diagnosis and guide preventative activities against these vector-borne diseases.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S1201971221001557Test; https://doaj.org/toc/1201-9712Test

الوصول الحر: https://doaj.org/article/663c0dbf9dec47ee8694e11805c47723Test

المؤلفون: Tadaki Suzuki, Souichi Yamada, Shuetsu Fukushi, Hitomi Kinoshita, Makoto Ohnishi, Tsuguto Fujimoto, Masayuki Saijo, Ken Maeda, Nozomu Hanaoka, Naomi Nojiri, Ai Kawana-Tachikawa, Shigeru Kusagawa, Koichi Ishikawa, Shigeyoshi Harada, Saori Matsuoka, Tadashi Kikuchi, Sayuri Seki, Midori Nakamura-Hoshi, Shoji Miki, Lucky Ronald Runtuwene, Nobuo Koizumi, Sunao Iyoda, Hideyuki Takahashi, Hidemasa Izumiya, Jiro Mitobe, Shouji Yamamoto, Masatomo Morita, Ken-ichi Lee, Ken Shimuta, Kyoko Saito, Masayoshi Fukasawa, Yasutaka Hoshino, Ken Miyazawa, Minoru Nagi, Chikako Shimokawa, Yasuyuki Morishima, Takashi Sakudoh, Yoshihiro Kaku, Chang Kweng Lim, Shigeru Tajima, Takahiro Maeki, Eri Nakayama, Satoshi Taniguchi, Motohiko Ogawa, Takanobu Kato, Hussein Hassan Aly, Kousho Wakae, Kento Fukano

المصدر: BMJ Open Respiratory Research, Vol 8, Iss 1 (2021)

مصطلحات موضوعية: Medicine, Diseases of the respiratory system, RC705-779

الوصف: Background An outbreak of novel coronavirus (SARS-CoV-2)-associated respiratory infectious diseases (COVID-19) emerged in 2019 and has spread rapidly in humans around the world. The demonstration of in vitro infectiousness of respiratory specimens is an informative surrogate for SARS-CoV-2 transmission from patients with COVID-19; accordingly, viral isolation assays in cell culture are an important aspect of laboratory diagnostics for COVID-19.Methods We developed a simple and rapid protocol for isolating SARS-CoV-2 from respiratory specimens using VeroE6/TMPRSS2 cells, a cell line that is highly susceptible to the virus. We also investigated a correlation between isolation of SARS-CoV-2 and viral load detected by real-time RT-PCR (rRT-PCR) using N2 primer/probe set that has been developed for testing of COVID-19 in Japan.Results The SARS-CoV-2 isolation protocol did not require blind passage of inoculated cells and yielded the results of viral isolation within 7 days after inoculation. Specimens with cycle threshold (Ct) values of 35 were virus isolation-positive, indicating that low viral loads (high Ct values) in upper respiratory specimens do not always indicate no risk of containing transmissible virus.Conclusion In combination with rRT-PCR, the SARS-CoV-2 isolation protocol provides a means for assessing the potential risk of transmissible virus in upper respiratory specimens.

وصف الملف: electronic resource

العلاقة: https://bmjopenrespres.bmj.com/content/8/1/e000830.fullTest; https://doaj.org/toc/2052-4439Test

الوصول الحر: https://doaj.org/article/b9d2ad04ff3d4af5a4c6a118be36fca9Test

المؤلفون: Hitomi Kinoshita, Kazuo Nakamichi, Chang-Kweng Lim, Mutsuyo Takayama-Ito, Lixin Wang, Itoe Iizuka, Ichiro Kurane, Masayuki Saijo

المصدر: Virology Journal, Vol 15, Iss 1, Pp 1-11 (2018)

مصطلحات موضوعية: LAMP, PML, JC polyomavirus, Detection, Quantification, Infectious and parasitic diseases, RC109-216

الوصف: Abstract Background JC polyomavirus (JCV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a demyelinating disease of the central nervous system in immunosuppressed patients. PML usually has a poor prognosis. Detection and quantification of the JCV genome in cerebrospinal fluid (CSF) is an efficacious tool for the diagnosis and management of PML, for which proper therapeutic interventions are required. Methods A loop-mediated isothermal amplification (LAMP) assay was applied for the quantitative detection of JCV. The LAMP assay was evaluated for the efficacy in diagnosis of PML in comparison with the TaqMan-based quantitative real-time PCR (qPCR) assay using 153 CSF specimens collected from patients with suspected PML. Results The LAMP assay showed no cross-reactivity against other polyomavirus plasmids, viral DNA, and viral RNA, which causes encephalitis, and detected 1 copy of the standard DNA per reaction. Among 50 qPCR-positives, 42 specimens (containing JCV genome ranged from 3.2 × 100 to 3.2 × 106 copies/reaction) showed positive reactions and 8 specimens (containing 0.9 to 19.9 copies/reaction) showed negative in the LAMP assay. Furthermore, 3 of 103 qPCR-negative specimens showed positive reactions in the LAMP assay. The sensitivity, specificity, positive predictive value, and negative predictive values of the LAMP assay were 84% (42/50), 97% (100/103), 93% (42/45), and 93% (100/108), respectively. The kappa statistic was 0.83. The JCV loads determined by the LAMP assay showed a strong positive correlation with those determined by the qPCR assay for 33 specimens with copy numbers of ≥1 copies/reaction (r = 0.89). Additionally, the LAMP assay could monitor the JCV genome copy number in CSF for sequential samples equivalently to qPCR assay. Conclusions The newly developed LAMP assay is highly specific against JCV and detect the JCV genome in the sample DNA containing 20 or more copies of JCV genome per reaction with 100% sensitivity (n = 29), which corresponds to ≥3 × 103 copies/mL of CSF. The LAMP assay is useful for the diagnosis and offers valuable information for the evaluation and management of PML in the clinical setting.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12985-018-1046-zTest; https://doaj.org/toc/1743-422XTest

الوصول الحر: https://doaj.org/article/a2545ed356524ae6bb23a8865de86c32Test

المؤلفون: Shiori Sano, Shuetsu Fukushi, Souichi Yamada, Shizuko Harada, Hitomi Kinoshita, Satoko Sugimoto, Tomoki Yoshikawa, Takeshi Kurosu, Yuki Takamatsu, Masayuki Shimojima, Shoichi Toda, Yuka Hamada, Naoki Fujisawa, Takayuki Sugimoto, Masayuki Saijo

المصدر: Viruses, Vol 13, Iss 4, p 693 (2021)

مصطلحات موضوعية: SFTS virus, RT-LAMP, simplified method, Microbiology, QR1-502

الوصف: Detection of severe fever with thrombocytopenia syndrome (SFTS) virus (SFTSV) during the early phase of the disease is important for appropriate treatment, infection control, and prevention of further transmission. The reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a nucleic acid amplification method that amplifies the target sequence under isothermal conditions. Here, we developed an RT-LAMP with a novel primer/probe set targeting a conserved region of the SFTSV L segment after extraction of viral RNA (standard RT-LAMP). Both the Chinese and Japanese SFTSV strains, including various genotypes, were detected by the standard RT-LAMP. We also performed RT-LAMP using the same primer/probe set but without the viral RNA extraction step (called simplified RT-LAMP) and evaluated the diagnostic efficacy. The sensitivity and specificity of the simplified RT-LAMP were 84.9% (45/53) and 89.5% (2/19), respectively. The simplified RT-LAMP can detect SFTSV in human sera containing >103.5 copies/mL viral RNA. The two RT-LAMP positive but quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) negative samples were positive in the conventional RT-PCR, suggesting that there was no false positive reaction in the RT-LAMP. Both the standard and simplified RT-LAMP are useful for detecting the SFTSV genome in patients during the early phase of the disease.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1999-4915/13/4/693Test; https://doaj.org/toc/1999-4915Test

الوصول الحر: https://doaj.org/article/d91a3cb6a59e4c7989ef6c0a17ca85e6Test

المؤلفون: Fumio Seki, Masahiro Miyoshi, Tatsuya Ikeda, Haruna Nishijima, Miwako Saikusa, Masae Itamochi, Hiroko Minagawa, Takako Kurata, Rei Ootomo, Jumboku Kajiwara, Takashi Kato, Katsuhiro Komase, Keiko Tanaka-Taya, Tomimasa Sunagawa, Kazunori Oishi, Nobuhiko Okabe, Hirokazu Kimura, Shigeru Suga, Kunihisa Kozawa, Noriyuki Otsuki, Yoshio Mori, Komei Shirabe, Makoto Takeda, Measles Virus Surveillance Group of Japan, Technical Support Team for Measles Control in Japan, Rika Komagome, Asami Ohnishi, Hiroyuki Saito, Mie Sasaki, Kenichi Komabayashi, Atsuko Kanari, Tsutomu Tamura, Kazunari Yamamoto, Kanako Ishikawa, Fuminori Mizukoshi, Hiroyuki Tsukagoshi, Yasutaka Ogawa, Takashi Nakada, Ai Kasuga, Tomoko Ogawa, Hajime Yokoi, Rieko Suzuki, Hideaki Shimizu, Satoko Kanazawa, Masayuki Oonuma, Nagano Environ, Kanako Nishizawa, Yuichiro Okamura, Asaka Ikegaya, Takaharu Maehata, Toshihiko Furuta, Masaya Nakazawa, Yoshihiro Yasui, Shinichiro Shibata, Tsuyoshi Kuzuguchi, Yasunori Tanaka, Hajime Kusuhara, Kayo Aoki, Sachi Hirata, Akiko Nagasao, Daiki Kanbayashi, Atsushi Kaida, Tatsuya Miyoshi, Miki Ogi, Taku Uemura, Shinya Kawanishi, Masaki Hiragakiuchi, Machi Inada, Takashi Nishiyama, Chika Tatsumi, Masako Hamano, Naoki Shigemoto, Fujii Yoshiki, Sachiko Murata, Yukari Terajima, Yumiko Kawakami, Yuki Ashizuka, Chinami Wasano, Misato Tachibana, Akiko Honda, Takashi Sakai, Kaori Nishizawa, Yu Matsuura, Mutsuyo Gokuden, Minori Oyama, Kenji Someya, Yuichiro Nakatsu, Maino Tahara, Kouji Sakai, Yukari Yamada, Kumi Ueno-Yamamoto, Yuki Tada, Tomoe Shimada, Kazuyo Yamashita, Hitomi Kinoshita, Takuri Takahashi, Kazutoshi Nakashima, Hajime Kamiya, Kiyosu Taniguchi, Yoshinori Yasui

المصدر: Frontiers in Microbiology, Vol 10 (2019)

مصطلحات موضوعية: measles virus, molecular epidemiology, genotype, elimination, Japan, Microbiology, QR1-502

الوصف: Genotyping evidence that supports the interruption of endemic measles virus (MV) transmission is one of the essential criteria to be verified in achieving measles elimination. In Japan since 2014, MV genotype analyses have been performed for most of the measles cases in prefectural public health institutes nationwide. With this strong molecular epidemiological data, Japan was verified to have eliminated measles in March, 2015. However, even in the postelimination era, sporadic cases and small outbreaks of measles have been detected repeatedly in Japan. This study investigated the nationwide molecular epidemiology of MV between 2008 and 2017. The 891 strains in the total period between 2008 and 2017 belonged to seven genotypes (D5, D4, D9, H1, G3, B3, and D8) and 124 different MV sequence variants, based on the 450-nucleotide sequence region of the N gene (N450). The 311 MV strains in the postelimination era between 2015 and 2017 were classified into 1, 7, 8, and 32 different N450 sequence variants in D9, H1, B3, and D8 genotypes, respectively. Analysis of the detection period of the individual N450 sequence variants showed that the majority of MV strains were detected only for a short period. However, MV strains, MVs/Osaka.JPN/29.15/ [D8] and MVi/Hulu Langat.MYS/26.11/ [D8], which are named strains designated by World Health Organization (WHO), have been detected in many cases over 2 or 3 years between 2015 and 2017. The WHO-named strains have circulated worldwide, causing outbreaks in many countries. Epidemiological investigation revealed repeated importation of these WHO-named strains into Japan. To demonstrate the elimination status (interruption of endemic transmission) in situations with repeated importation of the same strains is challenging. Nevertheless, the detailed sequence analysis of individual MV strains and chronological analysis of these strains provided sufficient evidence to show that Japan has still maintained its measles elimination status in 2017.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/article/10.3389/fmicb.2019.01470/fullTest; https://doaj.org/toc/1664-302XTest

الوصول الحر: https://doaj.org/article/71b1dae1082c40a8b41b4f5870bb953cTest

المؤلفون: Aika Watanabe, Yusuke Kobayashi, Tomoe Shimada, Yuichiro Yahata, Ayako Kobayashi, Mizue Kanai, Yushi Hachisu, Munehisa Fukusumi, Hajime Kamiya, Takuri Takahashi, Yuzo Arima, Hitomi Kinoshita, Kazuhiko Kanou, Takehito Saitoh, Satoru Arai, Hiroshi Satoh, Hideo Okuno, Saeko Morino, Tamano Matsui, Tomimasa Sunagawa, Keiko Tanaka-Taya, Makoto Takeda, Katsuhiro Komase, Kazunori Oishi

المصدر: Western Pacific Surveillance and Response, Vol 8, Iss 1, Pp 37-39 (2017)

مصطلحات موضوعية: measles, elimination, outbreaks, surveillance, genotyping, sequence analysis, Medicine, Public aspects of medicine, RA1-1270

وصف الملف: electronic resource

العلاقة: http://ojs.wpro.who.int/ojs/index.php/wpsar/article/view/517/740Test; https://doaj.org/toc/2094-7321Test; https://doaj.org/toc/2094-7313Test

الوصول الحر: https://doaj.org/article/9d83eca4401641b59cca421c05c15382Test