المؤلفون: Konaka, Hachiro, Kato, Yasuhiro, Hirano, Toru, Tsujimoto, Kohei, Park, JeongHoon, Koba, Taro, Aoki, Wataru, Matsuzaki, Yusei, Taki, Masayasu, Koyama, Shohei, Itotagawa, Eri, Jo, Tatsunori, Hirayama, Takehiro, Kawai, Taro, Ishii, Ken J, Ueda, Mitsuyoshi, Yamaguchi, Shigehiro, Akira, Shizuo, Morita, Takayoshi, Maeda, Yuichi, Nishide, Masayuki, Nishida, Sumiyuki, Shima, Yoshihito, Narazaki, Masashi, Takamatsu, Hyota, Kumanogoh, Atsushi

المساهمون: Japan Agency for Medical Research and Development, Japan Society for the Promotion of Science, Center of Innovation Program, Core Research for Evolutional Science and Technology, Mitsubishi Foundation, Naito Foundation

المصدر: The EMBO Journal ; volume 42, issue 20 ; ISSN 0261-4189 1460-2075

مصطلحات موضوعية: General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, Molecular Biology, General Neuroscience

الوصف: Mitochondrial DNA (mtDNA) leakage into the cytoplasm can occur when cells are exposed to noxious stimuli. Specific sensors recognize cytoplasmic mtDNA to promote cytokine production. Cytoplasmic mtDNA can also be secreted extracellularly, leading to sterile inflammation. However, the mode of secretion of mtDNA out of cells upon noxious stimuli and its relevance to human disease remain unclear. Here, we show that pyroptotic cells secrete mtDNA encapsulated within exosomes. Activation of caspase‐1 leads to mtDNA leakage from the mitochondria into the cytoplasm via gasdermin‐D. Caspase‐1 also induces intraluminal membrane vesicle formation, allowing for cellular mtDNA to be taken up and secreted as exosomes. Encapsulation of mtDNA within exosomes promotes a strong inflammatory response that is ameliorated upon exosome biosynthesis inhibition in vivo . We further show that monocytes derived from patients with Behçet's syndrome (BS), a chronic systemic inflammatory disorder, show enhanced caspase‐1 activation, leading to exosome‐mediated mtDNA secretion and similar inflammation pathology as seen in BS patients. Collectively, our findings support that mtDNA‐containing exosomes promote inflammation, providing new insights into the propagation and exacerbation of inflammation in human inflammatory diseases.

الإتاحة: https://doi.org/10.15252/embj.2022112573Test

المؤلفون: Okita, Yasutaka, Hirano, Toru, Wang, Bowen, Nakashima, Yuta, Minoda, Saki, Nagahara, Hajime, Kumanogoh, Atsushi

المساهمون: national institute of biomedical innovation, health and nutrition

المصدر: Arthritis Research & Therapy ; volume 25, issue 1 ; ISSN 1478-6362

الوصف: Background This work aims to develop a deep learning model, assessing atlantoaxial subluxation (AAS) in rheumatoid arthritis (RA), which can often be ambiguous in clinical practice. Methods We collected 4691 X-ray images of the cervical spine of the 906 patients with RA. Among these images, 3480 were used for training the deep learning model, 803 were used for validating the model during the training process, and the remaining 408 were used for testing the performance of the trained model. The two-dimensional key points’ detection model of Deep High-Resolution Representation Learning for Human Pose Estimation was adopted as the base convolutional neural network model. The model inferred four coordinates to calculate the atlantodental interval (ADI) and space available for the spinal cord (SAC). Finally, these values were compared with those by clinicians to evaluate the performance of the model. Results Among the 408 cervical images for testing the performance, the trained model correctly identified the four coordinates in 99.5% of the dataset. The values of ADI and SAC were positively correlated among the model and two clinicians. The sensitivity of AAS diagnosis with ADI or SAC by the model was 0.86 and 0.97 respectively. The specificity of that was 0.57 and 0.5 respectively. Conclusions We present the development of a deep learning model for the evaluation of cervical lesions of patients with RA. The model was demonstrably shown to be useful for quantitative evaluation.

الإتاحة: https://doi.org/10.1186/s13075-023-03172-xTest

المؤلفون: Makimoto, Kiyoko, Shiozawa, Kazuko, Shima, Yoshihito, Hirano, Toru, Inoue, Mitsuyo

المصدر: Open Journal of Nursing ; volume 13, issue 05, page 314-328 ; ISSN 2162-5336 2162-5344

مصطلحات موضوعية: General Medicine

الإتاحة: https://doi.org/10.4236/ojn.2023.135021Test

المؤلفون: Inoue, Mitsuyo, Shiozawa, Kazuko, Yoshihara, Ryosuke, Shima, Yoshihito, Hirano, Toru, Makimoto, Kiyoko

المصدر: Open Journal of Nursing ; volume 13, issue 01, page 22-44 ; ISSN 2162-5336 2162-5344

مصطلحات موضوعية: General Medicine

الإتاحة: https://doi.org/10.4236/ojn.2023.131002Test

المؤلفون: Sato, Masayuki, Ohashi, Masayuki, Tashi, Hideki, Makino, Tatsuo, Shibuya, Yohei, Hirano, Toru, Watanabe, Kei

المصدر: Journal of Orthopaedic Science ; volume 28, issue 6, page 1221-1226 ; ISSN 0949-2658

مصطلحات موضوعية: Orthopedics and Sports Medicine, Surgery

الإتاحة: https://doi.org/10.1016/j.jos.2022.10.001Test
https://api.elsevier.com/content/article/PII:S0949265822002858?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0949265822002858?httpAccept=text/plainTest

المؤلفون: Watanabe, Kei, Ohashi, Masayuki, Sekimoto, Hiroyuki, Tashi, Hideki, Shibuya, Yohei, Makino, Tatsuo, Hasegawa, Kazuhiro, Hirano, Toru

المصدر: Journal of Orthopaedic Science ; volume 28, issue 3, page 529-535 ; ISSN 0949-2658

مصطلحات موضوعية: Orthopedics and Sports Medicine, Surgery

الإتاحة: https://doi.org/10.1016/j.jos.2022.01.015Test
https://api.elsevier.com/content/article/PII:S0949265822000288?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0949265822000288?httpAccept=text/plainTest

المؤلفون: Ohashi, Masayuki, Watanabe, Kei, Hirano, Toru, Hasegawa, Kazuhiro, Tashi, Hideki, Makino, Tatsuo, Minato, Keitaro, Sato, Masayuki, Kawashima, Hiroyuki

المصدر: European Spine Journal; Jun2024, Vol. 33 Issue 6, p2522-2529, 8p

مستخلص: Purpose: We aimed to determine the clinical significance of neck and shoulder pain (NSP) 10 years after posterior spinal fusion (PSF) for thoracic adolescent idiopathic scoliosis (AIS) and the relationship between radiographic parameters and NSP. Methods: Of 72 patients who underwent PSF for thoracic AIS (Lenke 1 or 2) between 2000 and 2013, we included 52 (46 females; Lenke type 1 in 34 patients and type 2 in 18; mean age, 25.6 years) who underwent NSP evaluation using visual analog scale (VAS, 10 cm) 10 years postoperatively (follow-up rate, 72.2%). Correlation analyses were performed using Spearman's rank correlation coefficient (r). Results: The VAS for NSP was 2.6 cm in median and 3.4 cm in mean at 10 years. The VAS had significant negative correlations with several SRS-22 domain scores (rs = − 0.348 for pain, − 0.347 for function, − 0.308 for mental health, and − 0.372 for total) (p < 0.05). In addition, the VAS score was significantly correlated with cervical lordosis (CL) (rs = 0.296), lumbar lordosis (rs = − 0.299), and sacral slope (rs = 0.362) (p < 0.05). Furthermore, at the 10-year follow-up, CL was significantly negatively correlated with T1 slope (rs = − 0.763) and thoracic kyphosis (TK) (− 0.554 for T1-12 and − 0.344 for T5-12) (p < 0.02). Conclusion: NSP was associated with deterioration in SRS-22 scores, indicating that NSP is a clinically significant long-term issue in PSF for thoracic AIS. Restoring or maintaining the TK and T1 slopes, which are controllable factors during PSF, may improve cervical lordosis and alleviate NSP at 10-year follow-up. [ABSTRACT FROM AUTHOR]

: Copyright of European Spine Journal is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Noto, Yoshiyuki, Endo, Yuki, Ohashi, Masayuki, Hirano, Toru, Kuramoto, Tatsuya, Chida, Koichi, Watanabe, Kei

المصدر: European Spine Journal ; ISSN 0940-6719 1432-0932

مصطلحات موضوعية: Orthopedics and Sports Medicine, Surgery

الإتاحة: https://doi.org/10.1007/s00586-023-08006-7Test

المؤلفون: Ohtsubo, Shuhei, Ohashi, Masayuki, Hirano, Toru, Tashi, Hideki, Makino, Tatsuo, Minato, Keitaro, Mitsuma, Yusuke, Deguchi, Hiroyuki, Hoshino, Rintaro, Ohashi, Nobuko, Furutani, Kenta, Kawashima, Hiroyuki, Watanabe, Kei

المصدر: Spinal Cord Series & Cases; 4/17/2024, Vol. 10 Issue 1, p1-6, 6p

المؤلفون: Nakayama, Yoichi, Watanabe, Ryu, Yamamoto, Wataru, Ebina, Kosuke, Hirano, Toru, Kotani, Takuya, Shiba, Hideyuki, Katayama, Masaki, Son, Yonsu, Amuro, Hideki, Onishi, Akira, Jinno, Sadao, Hara, Ryota, Murakami, Kosaku, Murata, Koichi, Ito, Hiromu, Tanaka, Masao, Matsuda, Shuichi, Morinobu, Akio, Hashimoto, Motomu

المصدر: Rheumatology; Feb2024, Vol. 63 Issue 2, p349-357, 9p

مصطلحات موضوعية: INTERLEUKINS, DISEASE progression, RESEARCH, DATABASES, HEMOGLOBINS, SCIENTIFIC observation, MEDICAL information storage & retrieval systems, JANUS kinases, ANTIRHEUMATIC agents, TREATMENT effectiveness, RHEUMATOID arthritis, ANEMIA, TUMOR necrosis factors, DESCRIPTIVE statistics, KAPLAN-Meier estimator, RESEARCH funding, T cells, PROPORTIONAL hazards models, CHEMICAL inhibitors, DISEASE complications

مصطلحات جغرافية: JAPAN

مستخلص: Objectives Anaemia, a common comorbidity of RA, is related to high disease activity and poor prognosis. It is unknown which biologic/targeted synthetic (b/ts)-DMARDs are optimal for patients with anaemia and RA in regulating anaemia and controlling disease activity. Methods We investigated the change in haemoglobin (Hb) levels, drug retention rates and disease activities after the administration of b/ts-DMARDs with different modes of action [TNF inhibitors (TNFis), immunoglobulin fused with cytotoxic T-lymphocyte antigen (CTLA-4-Ig), IL-6 receptor inhibitors (IL-6Ris) and Janus kinase inhibitors (JAKis)] in patients with RA stratified by baseline Hb levels using the multicentre observational registry for patients with RA in Japan (ANSWER cohort). Results A total of 2093 patients with RA were classified into three groups based on tertiles of the baseline Hb levels (Hb^low, anaemic; Hb^int, intermediate; Hb^high, non-anaemic). IL-6Ri increased Hb levels in all groups (the mean change at 12 months in Hb^low was +1.5 g/dl, Hb^int +0.7 g/dl and Hb^high +0.1 g/dl). JAKis increased the Hb level in patients with anaemia and RA and retained or decreased the Hb level in non-anaemic patients (the mean change at 12 months in Hb^low was +0.6 g/dl, Hb^int 0 g/dl and Hb^high −0.3 g/dl). In patients with anaemia and RA, overall adjusted 3-year drug retention rates were higher in JAKi followed by IL-6Ri, CTLA4-Ig and TNFi (78.6%, 67.9%, 61.8% and 50.8%, respectively). Change of disease activity at 12 months was not different among different b/ts-DMARDs treatments. Conclusion IL-6Ri and JAKi can effectively treat patients with anaemia and RA in a real-world setting. [ABSTRACT FROM AUTHOR]

: Copyright of Rheumatology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)