المؤلفون: Kobiela, Adrian, Hewelt-Belka, Weronika, Frąckowiak, Joanna E., Kordulewska, Natalia, Hovhannisyan, Lilit, Bogucka, Aleksandra, Etherington, Rachel, Piróg, Artur, Dapic, Irena, Gabrielsson, Susanne, Brown, Sara J., Ogg, Graham S., Gutowska-Owsiak, Danuta

المساهمون: Fundacja na rzecz Nauki Polskiej, Narodowe Centrum Nauki, Medical Research Council, NIHR Oxford Biomedical Research Centre, Wellcome Trust, Medicinska Forskningsrådet, Karolinska Institutet

المصدر: Frontiers in Immunology ; volume 15 ; ISSN 1664-3224

مصطلحات موضوعية: Immunology, Immunology and Allergy

الوصف: Introduction Exosome-enriched small extracellular vesicles (sEVs) are nanosized organelles known to participate in long distance communication between cells, including in the skin. Atopic dermatitis (AD) is a chronic inflammatory skin disease for which filaggrin ( FLG ) gene mutations are the strongest genetic risk factor. Filaggrin insufficiency affects multiple cellular function, but it is unclear if sEV-mediated cellular communication originating from the affected keratinocytes is also altered, and if this influences peptide and lipid antigen presentation to T cells in the skin. Methods Available mRNA and protein expression datasets from filaggrin-insufficient keratinocytes (shFLG), organotypic models and AD skin were used for gene ontology analysis with FunRich tool. sEVs secreted by shFLG and control shC cells were isolated from conditioned media by differential centrifugation. Mass spectrometry was carried out for lipidomic and proteomic profiling of the cells and sEVs. T cell responses to protein, peptide, CD1a lipid antigens, as well as phospholipase A2-digested or intact sEVs were measured by ELISpot and ELISA. Results Data analysis revealed extensive remodeling of the sEV compartment in filaggrin insufficient keratinocytes, 3D models and the AD skin. Lipidomic profiles of shFLGsEV showed a reduction in the long chain (LCFAs) and polyunsaturated fatty acids (PUFAs; permissive CD1a ligands) and increased content of the bulky headgroup sphingolipids (non-permissive ligands). This resulted in a reduction of CD1a-mediated interferon-γ T cell responses to the lipids liberated from shFLG-generated sEVs in comparison to those induced by sEVs from control cells, and an increase in interleukin 13 secretion. The altered sEV lipidome reflected a generalized alteration in the cellular lipidome in filaggrin-insufficient cells and the skin of AD patients, resulting from a downregulation of key enzymes implicated in fatty acid elongation and desaturation, i.e., enzymes of the ACSL, ELOVL and FADS family. Discussion We ...

الإتاحة: https://doi.org/10.3389/fimmu.2024.1369238Test

المؤلفون: Griffante Gloria, Hewelt-Belka Weronika, Albano Camilla, Gugliesi Francesca, Pasquero Selina, Castillo Pacheco Sergio F., Bajetto Greta, Trifiro' Linda, Porporato Paolo E., Mina Erica, Vallino Marta, Krapp Christian, Jakobsen Martin R., Purdy John, Von Einem Jens, Landolfo Santo, Dell'oste Valentina, Biolatti Matteo

المساهمون: Griffante Gloria, Hewelt-Belka Weronika, Albano Camilla, Gugliesi Francesca, Pasquero Selina, Castillo Pacheco Sergio F., Bajetto Greta, Trifiro' Linda, Porporato Paolo E., Mina Erica, Vallino Marta, Krapp Christian, Jakobsen Martin R., Purdy John, Von Einem Jens, Landolfo Santo, Dell'oste Valentina, Biolatti Matteo

العلاقة: ispartofbook:50° Congresso Nazionale della Società Italiana di Microbiologia - Abstract Book; 50° Congresso Nazionale della Società Italiana di Microbiologia; firstpage:45; lastpage:45; numberofpages:1; http://hdl.handle.net/2318/1876818Test

الإتاحة: http://hdl.handle.net/2318/1876818Test

المؤلفون: Hewelt-Belka, Weronika, Młynarczyk, Michał, Garwolińska, Dorota, Kot-Wasik, Agata

المساهمون: Narodowe Centrum Nauki

المصدر: Journal of Agricultural and Food Chemistry ; volume 71, issue 46, page 17899-17908 ; ISSN 0021-8561 1520-5118

مصطلحات موضوعية: General Agricultural and Biological Sciences, General Chemistry

الإتاحة: https://doi.org/10.1021/acs.jafc.3c04489Test

المؤلفون: Woziński, Mateusz, Greber, Katarzyna Ewa, Pastewska, Monika, Kolasiński, Piotr, Hewelt-Belka, Weronika, Żołnowska, Beata, Sławiński, Jarosław, Szulczyk, Daniel, Sawicki, Wiesław, Ciura, Krzesimir

المساهمون: Narodowe Centrum Nauki, Narodowym Centrum Nauki, Ministerstwo Edukacji i Nauki

المصدر: Journal of Pharmaceutical and Biomedical Analysis ; volume 239, page 115916 ; ISSN 0731-7085

مصطلحات موضوعية: Clinical Biochemistry, Spectroscopy, Drug Discovery, Pharmaceutical Science, Analytical Chemistry

الإتاحة: https://doi.org/10.1016/j.jpba.2023.115916Test
https://api.elsevier.com/content/article/PII:S0731708523006854?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0731708523006854?httpAccept=text/plainTest

المؤلفون: Kłosowska-Chomiczewska, Ilona E., Macierzanka, Adam, Parchem, Karol, Miłosz, Pamela, Bladowska, Sonia, Płaczkowska, Iga, Hewelt-Belka, Weronika, Jungnickel, Christian

المصدر: Scientific Reports; 4/10/2024, Vol. 14 Issue 1, p1-16, 16p

مصطلحات موضوعية: SOLUBILIZATION, SURFACE active agents, RHAMNOLIPIDS, BIOSYNTHESIS, MICROORGANISMS

مستخلص: In the growing landscape of interest in natural surfactants, selecting the appropriate one for specific applications remains challenging. The extensive, yet often unsystematized, knowledge of microbial surfactants, predominantly represented by rhamnolipids (RLs), typically does not translate beyond the conditions presented in scientific publications. This limitation stems from the numerous variables and their interdependencies that characterize microbial surfactant production. We hypothesized that a computational recipe for biosynthesizing RLs with targeted applicational properties could be developed from existing literature and experimental data. We amassed literature data on RL biosynthesis and micellar solubilization and augmented it with our experimental results on the solubilization of triglycerides (TGs), a topic underrepresented in current literature. Utilizing this data, we constructed mathematical models that can predict RL characteristics and solubilization efficiency, represented as logPRL = f(carbon and nitrogen source, parameters of biosynthesis) and logMSR = f(solubilizate, rhamnolipid (e.g. logPRL), parameters of solubilization), respectively. The models, characterized by robust R² values of respectively 0.581–0.997 and 0.804, enabled the ranking of descriptors based on their significance and impact—positive or negative—on the predicted values. These models have been translated into ready-to-use calculators, tools designed to streamline the selection process for identifying a biosurfactant optimally suited for intended applications. [ABSTRACT FROM AUTHOR]

: Copyright of Scientific Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Griffante, Gloria, Hewelt-Belka, Weronika, Albano, Camilla, Gugliesi, Francesca, Pasquero, Selina, Castillo Pacheco, Sergio Fernando, Bajetto, Greta, Porporato, Paolo Ettore, Mina, Erica, Vallino, Marta, Krapp, Christian, Jakobsen, Martin Roelsgaard, Purdy, John, von Einem, Jens, Landolfo, Santo, Dell'Oste, Valentina, Biolatti, Matteo

المصدر: Griffante , G , Hewelt-Belka , W , Albano , C , Gugliesi , F , Pasquero , S , Castillo Pacheco , S F , Bajetto , G , Porporato , P E , Mina , E , Vallino , M , Krapp , C , Jakobsen , M R , Purdy , J , von Einem , J , Landolfo , S , Dell'Oste , V & Biolatti , M 2022 , ' IFI16 Impacts Metabolic Reprogramming during Human Cytomegalovirus Infection ' , mBio , vol. 13 , no. 3 , pp. e0043522 . https://doi.org/10.1128/mbio.00435-22Test

مصطلحات موضوعية: ACTIVATION, ANTIBODY, ATHEROSCLEROSIS, CHOLESTEROL, CHREBP, EXPRESSION, IFI16, KINASE, PATHWAY, RISK-FACTOR, VIRUS-INFECTION, glucose and lipid metabolism, human cytomegalovirus, lipidomics, virus-host interactions, Cytomegalovirus/physiology, Humans, DNA, Viral/genetics, Cytomegalovirus Infections, Cellular Reprogramming, Phosphoproteins/genetics, Virus Replication, Fibroblasts, Nuclear Proteins/genetics

الوصف: Cellular lipid metabolism plays a pivotal role in human cytomegalovirus (HCMV) infection, as increased lipogenesis in HCMV-infected cells favors the envelopment of newly synthesized viral particles. As all cells are equipped with restriction factors (RFs) able to exert a protective effect against invading pathogens, we asked whether a similar defense mechanism would also be in place to preserve the metabolic compartment from HCMV infection. Here, we show that gamma interferon (IFN-γ)-inducible protein 16 (IFI16), an RF able to block HCMV DNA synthesis, can also counteract HCMV-mediated metabolic reprogramming in infected primary human foreskin fibroblasts (HFFs), thereby limiting virion infectivity. Specifically, we find that IFI16 downregulates the transcriptional activation of the glucose transporter 4 (GLUT4) through cooperation with the carbohydrate-response element-binding protein (ChREBP), thereby reducing HCMV-induced transcription of lipogenic enzymes. The resulting decrease in glucose uptake and consumption leads to diminished lipid synthesis, which ultimately curbs the de novo formation of enveloped viral particles in infected HFFs. Consistently, untargeted lipidomic analysis shows enhanced cholesteryl ester levels in IFI16 KO versus wild-type (WT) HFFs. Overall, our data unveil a new role of IFI16 in the regulation of glucose and lipid metabolism upon HCMV replication and uncover new potential targets for the development of novel antiviral therapies. IMPORTANCE Human cytomegalovirus (HCMV) gathers all the substrates and enzymes necessary for the assembly of new virions from its host cell. For instance, HCMV is known to induce cellular metabolism of infected cells to favor virion assembly. Cells are, however, equipped with a first-line defense represented by restriction factors (RFs), which after sensing viral DNA can trigger innate and adaptive responses, thereby blocking HCMV replication. One such RF is IFN-γ-inducible protein 16 (IFI16), which we have shown to downregulate viral replication in human ...

الإتاحة: https://doi.org/10.1128/mbio.00435-22Test
https://pure.au.dk/portal/da/publications/ifi16-impacts-metabolic-reprogramming-during-human-cytomegalovirus-infectionTest(5d4327b6-142b-498e-b7d6-e6cfbfad3d83).html

المؤلفون: Garwolińska, Dorota, Młynarczyk, Michał, Kot-Wasik, Agata, Hewelt-Belka, Weronika

المساهمون: Narodowe Centrum Nauki

المصدر: Journal of Proteome Research ; volume 21, issue 2, page 438-446 ; ISSN 1535-3893 1535-3907

الإتاحة: https://doi.org/10.1021/acs.jproteome.1c00760Test

المؤلفون: Garwolińska, Dorota, Hewelt-Belka, Weronika, Kot-Wasik, Agata, Sundekilde, Ulrik Kræmer

المصدر: Garwolińska , D , Hewelt-Belka , W , Kot-Wasik , A & Sundekilde , U K 2020 , ' Nuclear Magnetic Resonance Metabolomics Reveals Qualitative and Quantitative Differences in the Composition of Human Breast Milk and Milk Formulas ' , Nutrients , vol. 12 , no. 4 , 921 . https://doi.org/10.3390/nu12040921Test

مصطلحات موضوعية: breastfeeding, foodomics, human breast milk, human milk composition, human milk metabolites, infant feeding, infant formula, NMR metabolomics

الوصف: Commercial formula milk (FM) constitutes the best alternative to fulfill the nutritional requirements of infants when breastfeeding is precluded. Here, we present the comparative study of polar metabolite composition of human breast milk (HBM) and seven different brands of FM by nuclear magnetic resonance spectroscopy. The results of the multivariate data analysis exposed qualitative and quantitative differences between HBM and FM composition as well as within FM of various brands and in HBM itself (between individual mothers and lactation period). Several metabolites were found exclusively in HBM and FM. Statistically significant higher levels of isoleucine and methionine in their free form were detected in FM samples based on caprine milk, while FM samples based on bovine milk showed a higher level of glucose and galactose in comparison to HBM. The results suggest that the amelioration of FM formulation is imperative to better mimic the composition of minor nutrients in HBM.

الإتاحة: https://doi.org/10.3390/nu12040921Test
https://pure.au.dk/portal/da/publications/nuclear-magnetic-resonance-metabolomics-reveals-qualitative-and-quantitative-differences-in-the-composition-of-human-breast-milk-and-milk-formulasTest(b29755b3-22fe-4cae-87ca-4c375a8e486b).html
http://www.scopus.com/inward/record.url?scp=85082730664&partnerID=8YFLogxKTest

المؤلفون: Bakhytkyzy, Inal, Hewelt-Belka, Weronika, Kot-Wasik, Agata

المساهمون: National Science Centre, Poland

المصدر: Microchemical Journal ; volume 152, page 104269 ; ISSN 0026-265X

مصطلحات موضوعية: Spectroscopy, Analytical Chemistry

الإتاحة: https://doi.org/10.1016/j.microc.2019.104269Test
https://api.elsevier.com/content/article/PII:S0026265X19319198?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0026265X19319198?httpAccept=text/plainTest

المؤلفون: Garwolińska, Dorota, Kot-Wasik, Agata, Hewelt-Belka, Weronika

المصدر: Molecular Omics ; volume 19, issue 2, page 95-104 ; ISSN 2515-4184

الوصف: We provide key information regarding the pre-analytical variables affecting the metabolomics studies of biological fluids.

الإتاحة: https://doi.org/10.1039/d2mo00212dTest
http://pubs.rsc.org/en/content/articlepdf/2023/MO/D2MO00212DTest