المؤلفون: Espeseth, Amy S., Yuan, Maoli, Citron, Michael, Reiserova, Lucia, Morrow, Gavin, Wilson, Aaron, Horton, Melanie, Rukhman, Mark, Kinek, Keith, Hou, Fuxiang, Li, Shui L., Li, Fengsheng, Choi, Yesle, Heidecker, Gwen, Luo, Bin, Wu, Guoxin, Zhang, Lan, Strable, Erica, DeStefano, Joanne, Secore, Susan, Mukhopadhyay, Tarit K., Richardson, Douglas D., Sayeed, Eddy, Welch, Lisa S., Bett, Andrew J., Feinberg, Mark B., Gupta, Swati B., Cooper, Christopher L., Parks, Christopher L.

المصدر: eBioMedicine ; volume 82, page 104203 ; ISSN 2352-3964

مصطلحات موضوعية: General Biochemistry, Genetics and Molecular Biology, General Medicine

الإتاحة: https://doi.org/10.1016/j.ebiom.2022.104203Test
https://api.elsevier.com/content/article/PII:S2352396422003851?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2352396422003851?httpAccept=text/plainTest

المؤلفون: Vora, Kalpit, Chen, Zhifeng, Su, Hua-Poo, Tang, Aimin, Cox, Kara, Callahan, Cheryl, Zhang, Lan, Patel, Sangita, Nahas, Debbie, Citron, Mike, Cejas, Pedro, Swoyer, Ryan, Luo, Bin, Eddins, Michael, Reid, John, Fridman, Arthur, Galli, Jennifer, Cosmi, Scott, Dhanasekeran, Govindarajan, Wen, Zhiyun, He, Xi, Wang, Dai, Heidecker, Gwen, Flynn, Jessica, Cook, James, Soisson, Stephen, Casimiro, Danilo, Bett, Andrew, Blair, Wade, Distefano, Daniel, Haines, Christopher

المصدر: Open Forum Infectious Diseases ; volume 5, issue suppl_1, page S47-S48 ; ISSN 2328-8957

مصطلحات موضوعية: Infectious Diseases, Oncology

الوصف: Background Respiratory syncytial virus (RSV) infection is a major public health burden for infants and the elderly worldwide. Currently, there are no approved vaccines and only one moderately effective marketed antibody (Synagis®) for the prevention of RSV infection in high-risk infants. Sampling the human antibody repertoire has led to the realization that the RSV fusion (F) protein in its prefusion conformation is the preferred target for potent neutralizing antibodies and thus makes the protein an attractive candidate for vaccine developers. Methods We report the isolation of a potent and broad RSV neutralizing monoclonal antibody (mAb), which was discovered through molecular cloning of cultured RSV postfusion F protein-baited single-sorted human memory B cells. The epitope for the mAb was mapped to RSV F protein using various methods; including X-ray crystallography, alanine scan, and RSV escape mutant generation Results The mAb binds to both RSV pre- and postfusion F proteins at site IV and can neutralize RSV A and B laboratory strains with subnanomolar potency, superior to that of Palivizumab. Antigenic site IV is conserved between pre- and postfusion F proteins in both RSV A and B subgroups, and sequence alignment showed that the mAb-binding site was conserved in >1,000 RSV A and B clinical isolates. In vivo cotton rat studies demonstrated protection of both the upper and lower respiratory tract of antibody-infused animals challenged with either RSV A or RSV B. Conclusion Overall, the fully human mAb we have isolated has great potential to be developed for passive immune-prophylaxis in infants. A prevalent view of the RSV scientific community is that RSV neutralizing mAbs in human sera primarily target the prefusion F protein and predominantly bind antigenic site Ø. In contrast, our finding demonstrates that very broad and potent RSV neutralizing mAb can also recognize sites common to pre- and postfusion F proteins. Furthermore, the RSV F antigenic site IV presents a neutralizing epitope ...

الإتاحة: https://doi.org/10.1093/ofid/ofy209.114Test
http://academic.oup.com/ofid/article-pdf/5/suppl_1/S47/33595448/ofy209.114.pdfTest

المؤلفون: Fan, Jiang, Liang, Xiaoping, Horton, Melanie S, Perry, Helen C, Citron, Michael P, Heidecker, Gwen J, Fu, Tong-Ming, Joyce, Joseph, Przysiecki, Craig T, Keller, Paul M, Garsky, Victor M, Ionescu, Roxana, Rippeon, Yvette, Shi, Li, Chastain, Michael A, Condra, Jon H, Davies, Mary-Ellen, Liao, Jason, Emini, Emilio A, Shiver, John W

المصدر: Vaccine ; volume 22, issue 23-24, page 2993-3003 ; ISSN 0264-410X

مصطلحات موضوعية: Infectious Diseases, Public Health, Environmental and Occupational Health, General Veterinary, General Immunology and Microbiology, Molecular Medicine

الإتاحة: https://doi.org/10.1016/j.vaccine.2004.02.021Test
https://api.elsevier.com/content/article/PII:S0264410X04001835?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0264410X04001835?httpAccept=text/plainTest