المؤلفون: Ahmed Karmaoui, Abdelkrim Ben Salem, Samir El Jaafari, Hassan Chaachouay, Adil Moumane, Lhoussain Hajji

المصدر: Frontiers in Earth Science, Vol 10 (2022)

مصطلحات موضوعية: Drought, Water, Groundwater, dryland, vegetation cover, NDVI, Science

الوصف: The study investigates the land use and land cover (LULC) in Errachidia province (Pre-Saharan of Morocco) in the period 2005–2020. To this end, remote sensing (RS) tools such as LULC, the normalized difference vegetation index (NDVI), the normalized difference water index (NDWI), the Enhanced Vegetation Index (EVI), Gravity Recovery and Climate Experiment (GRACE) satellite data, and precipitations were processed and combined. The monitoring of LULC over this period reveals different changes in this area. Generally, for water bodies, two trends can be observed, an increasing trend since 2006 and a decreasing trend from 2011 to the present. However, an increasing trend was recorded for the urban-and-built-up-lands and the grasslands categories. NDVI, NDWI, and EVI showed three major peaks recorded in the same periods (in 2007, 2009, and 2015). In terms of water storage, three phases were found. The first depicted the lowest groundwater quantities with a decreasing trend, which corresponds to a period of drought and/or human pressure. The second phase, the most important that recorded the greatest storage of water while the third phase corresponds to low storage with a decreasing trend. LULC of croplands reveals a slightly increasing trend from 2012 to 2020, which shows an orientation to an extension of crops mainly of date palm encouraged by government programs. A strong correlation between the seasonal NDVI and water storage using GRACE-Data for the period was found. In the end, recommendations on the adaptation to CC were proposed. The findings demonstrate that RS techniques are useful tools to detect hydrological droughts, observe, and conserve land resources. In arid areas such as Errachidia, the solution lies in rationalizing the use of water resources protecting them from uncontrolled anthropogenic events, which may alleviate the pressure. To support local sustainable development, environmental scientists and decision-makers may use the outputs of this study.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/feart.2022.962097/fullTest; https://doaj.org/toc/2296-6463Test

الوصول الحر: https://doaj.org/article/cb2d1475945445008d06ad78b7c97745Test

المؤلفون: Hyrije Ademi, Dheeraj A Shinde, Max Gassmann, Daniela Gerst, Hassan Chaachouay, Johannes Vogel, Thomas A Gorr

المصدر: PLoS ONE, Vol 16, Iss 5, p e0251765 (2021)

مصطلحات موضوعية: Medicine, Science

الوصف: Since growing tumors stimulate angiogenesis, via vascular endothelial growth factor (VEGF), angiogenesis inhibitors (AIs, blockers of the VEGF signaling pathway) have been introduced to cancer therapy. However, AIs often yielded only modest and short-lived gains in cancer patients and more invasive tumor phenotypes in animal models. Combining anti-VEGF strategies with lactate uptake blockers may boost both efficacy and safety of AIs. We assessed this hypothesis by using the ex ovo chorioallantoic membrane (CAM) assay. We show that AI-based monotherapy (Avastin®, AVA) increases tumor hypoxia in human CAM cancer cell xenografts and cell spread in human as well as canine CAM cancer cell xenografts. In contrast, combining AVA treatment with lactate importer MCT1 inhibitors (α-cyano-4-hydroxycinnamic acid (CHC) or AZD3965 (AZD)) reduced both tumor growth and cell dissemination of human and canine explants. Moreover, combining AVA+AZD diminished blood perfusion and tumor hypoxia in human explants. Thus, the ex ovo CAM assay as an easy, fast and cheap experimental setup is useful for pre-clinical cancer research. Moreover, as an animal-free experimental setup the CAM assay can reduce the high number of laboratory animals used in pre-clinical cancer research.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1932-6203Test

الوصول الحر: https://doaj.org/article/4d0d39519819418880f3c2187cac7b0bTest

المؤلفون: Nadine Schulz, Hassan Chaachouay, Katarzyna J. Nytko, Mathias S. Weyland, Malgorzata Roos, Rudolf M. Füchslin, Franco Guscetti, Stephan Scheidegger, Carla Rohrer Bley

المصدر: International Journal of Molecular Sciences, Vol 18, Iss 6, p 1176 (2017)

مصطلحات موضوعية: DNA damage repair, kinetics, γH2AX-foci, comet assay, dog, radiation, DNA repair model, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: Time resolved data of DNA damage and repair after radiotherapy elucidates the relation between damage, repair, and cell survival. While well characterized in vitro, little is known about the time-course of DNA damage response in tumors sampled from individual patients. Kinetics of DNA damage after radiotherapy was assessed in eight dogs using repeated in vivo samples of tumor and co-irradiated normal tissue analyzed with comet assay and phosphorylated H2AX (γH2AX) immunohistochemistry. In vivo results were then compared (in silico) with a dynamic mathematical model for DNA damage formation and repair. Maximum %DNA in tail was observed at 15–60 min after irradiation, with a rapid decrease. Time-courses of γH2AX-foci paralleled these findings with a small time delay and were not influenced by covariates. The evolutionary parameter search based on %DNA in tail revealed a good fit of the DNA repair model to in vivo data for pooled sarcoma time-courses, but fits for individual sarcoma time-courses suffer from the heterogeneous nature of the in vivo data. It was possible to follow dynamics of comet tail intensity and γH2AX-foci during a course of radiation using a minimally invasive approach. DNA repair can be quantitatively investigated as time-courses of individual patients by integrating this resulting data into a dynamic mathematical model.

وصف الملف: electronic resource

العلاقة: http://www.mdpi.com/1422-0067/18/6/1176Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/39f380ff6ad14367a7982acf15be4fabTest

المؤلفون: Hyrije Ademi (10810512), Dheeraj A. Shinde (10810515), Max Gassmann (2951061), Daniela Gerst (10810518), Hassan Chaachouay (10810521), Johannes Vogel (515351), Thomas A. Gorr (10810524)

مصطلحات موضوعية: Biochemistry, Cell Biology, Molecular Biology, Pharmacology, Cancer, Chemical Sciences not elsewhere classified, VEGF, CHC, chick embryo chorioallantoic membranes, increases tumor hypoxia, ovo chorioallantoic membrane, cancer research, AVA, ovo CAM assay, CAM cancer cell xenografts, AZD, lactate uptake blockers, AI, MCT

الوصف: (PDF)

العلاقة: https://figshare.com/articles/journal_contribution/Tumor_tissue_hypoxia_on_paraffin_section_Pimonidazole_/14606346Test

الإتاحة: https://doi.org/10.1371/journal.pone.0251765.s005Test

المؤلفون: Hyrije Ademi (10810512), Dheeraj A. Shinde (10810515), Max Gassmann (2951061), Daniela Gerst (10810518), Hassan Chaachouay (10810521), Johannes Vogel (515351), Thomas A. Gorr (10810524)

مصطلحات موضوعية: Biochemistry, Cell Biology, Molecular Biology, Pharmacology, Cancer, Chemical Sciences not elsewhere classified, VEGF, CHC, chick embryo chorioallantoic membranes, increases tumor hypoxia, ovo chorioallantoic membrane, cancer research, AVA, ovo CAM assay, CAM cancer cell xenografts, AZD, lactate uptake blockers, AI, MCT

الوصف: (PDF)

العلاقة: https://figshare.com/articles/journal_contribution/In_vitro_drug_toxicity_assay_/14606343Test

الإتاحة: https://doi.org/10.1371/journal.pone.0251765.s004Test

المؤلفون: Hyrije Ademi (10810512), Dheeraj A. Shinde (10810515), Max Gassmann (2951061), Daniela Gerst (10810518), Hassan Chaachouay (10810521), Johannes Vogel (515351), Thomas A. Gorr (10810524)

مصطلحات موضوعية: Biochemistry, Cell Biology, Molecular Biology, Pharmacology, Cancer, Chemical Sciences not elsewhere classified, VEGF, CHC, chick embryo chorioallantoic membranes, increases tumor hypoxia, ovo chorioallantoic membrane, cancer research, AVA, ovo CAM assay, CAM cancer cell xenografts, AZD, lactate uptake blockers, AI, MCT

الوصف: (PDF)

العلاقة: https://figshare.com/articles/journal_contribution/S1_Raw_images_-/14606334Test

الإتاحة: https://doi.org/10.1371/journal.pone.0251765.s001Test

المؤلفون: Hyrije Ademi (10810512), Dheeraj A. Shinde (10810515), Max Gassmann (2951061), Daniela Gerst (10810518), Hassan Chaachouay (10810521), Johannes Vogel (515351), Thomas A. Gorr (10810524)

مصطلحات موضوعية: Biochemistry, Cell Biology, Molecular Biology, Pharmacology, Cancer, Chemical Sciences not elsewhere classified, VEGF, CHC, chick embryo chorioallantoic membranes, increases tumor hypoxia, ovo chorioallantoic membrane, cancer research, AVA, ovo CAM assay, CAM cancer cell xenografts, AZD, lactate uptake blockers, AI, MCT

الوصف: (PDF)

العلاقة: https://figshare.com/articles/journal_contribution/Primer_Probe_sequences_used_in_ddPCR_/14606337Test

الإتاحة: https://doi.org/10.1371/journal.pone.0251765.s002Test

المؤلفون: Hyrije Ademi (10810512), Dheeraj A. Shinde (10810515), Max Gassmann (2951061), Daniela Gerst (10810518), Hassan Chaachouay (10810521), Johannes Vogel (515351), Thomas A. Gorr (10810524)

مصطلحات موضوعية: Biochemistry, Cell Biology, Molecular Biology, Pharmacology, Cancer, Chemical Sciences not elsewhere classified, VEGF, CHC, chick embryo chorioallantoic membranes, increases tumor hypoxia, ovo chorioallantoic membrane, cancer research, AVA, ovo CAM assay, CAM cancer cell xenografts, AZD, lactate uptake blockers, AI, MCT

الوصف: (PDF)

العلاقة: https://figshare.com/articles/journal_contribution/Primer_used_in_SYBR_green_qPCR_/14606340Test

الإتاحة: https://doi.org/10.1371/journal.pone.0251765.s003Test

المؤلفون: Hyrije Ademi (10810512), Dheeraj A. Shinde (10810515), Max Gassmann (2951061), Daniela Gerst (10810518), Hassan Chaachouay (10810521), Johannes Vogel (515351), Thomas A. Gorr (10810524)

مصطلحات موضوعية: Biochemistry, Cell Biology, Molecular Biology, Pharmacology, Cancer, Chemical Sciences not elsewhere classified, VEGF, CHC, chick embryo chorioallantoic membranes, increases tumor hypoxia, ovo chorioallantoic membrane, cancer research, AVA, ovo CAM assay, CAM cancer cell xenografts, AZD, lactate uptake blockers, AI, MCT

الوصف: (PDF)

العلاقة: https://figshare.com/articles/journal_contribution/Tumor_tissue_hypoxia_on_paraffin_section_Carbonic_anhydrase_IX_CA_IX_/14606349Test

الإتاحة: https://doi.org/10.1371/journal.pone.0251765.s006Test

المؤلفون: Hyrije Ademi (10810512), Dheeraj A. Shinde (10810515), Max Gassmann (2951061), Daniela Gerst (10810518), Hassan Chaachouay (10810521), Johannes Vogel (515351), Thomas A. Gorr (10810524)

مصطلحات موضوعية: Biochemistry, Cell Biology, Molecular Biology, Pharmacology, Cancer, Chemical Sciences not elsewhere classified, VEGF, CHC, chick embryo chorioallantoic membranes, increases tumor hypoxia, ovo chorioallantoic membrane, cancer research, AVA, ovo CAM assay, CAM cancer cell xenografts, AZD, lactate uptake blockers, AI, MCT

الوصف: (PDF)

العلاقة: https://figshare.com/articles/journal_contribution/Flux_measurement_on_D17_tumor_explants_treated_with_10-times_higher_AVA_concentration_/14606358Test

الإتاحة: https://doi.org/10.1371/journal.pone.0251765.s009Test