المؤلفون: Shuoqing Shi, Yufan Zhao, Haiou Yang, Chewang Jia, Haoyuan Deng, Tianchi Zhang, Xin Lin, Weidong Huang

المصدر: Materials Research Letters, Vol 12, Iss 9, Pp 668-677 (2024)

مصطلحات موضوعية: Additive manufacturing, AlSi10Mg alloy, high-speed scanning remelting, microstructure, strength-plasticity performance, Materials of engineering and construction. Mechanics of materials, TA401-492

الوصف: The limited mechanical properties of Al–Si alloys hinder their application in demanding and extreme conditions. The cracking tendency of high-strength aluminum alloys and high-cost of rare-earth elements pose challenges for the large-scale application of aluminum alloys in additive manufacturing. The novel and practical high-speed scanning remelting proposed in this study enables Al–Si alloys to exhibit significant proportion of fine microstructures and nano-precipitates, surpassing the mechanical properties of aluminum alloys fabricated by normal methods with exceptional strength (496.1 ± 5.8 MPa) and plasticity (21.4 ± 0.9%). This in-situ microstructure control method opens new pathways for the application in demanding engineering contexts.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2166-3831Test

الوصول الحر: https://doaj.org/article/bde638489bb94417bf25ba7aa2380656Test

المؤلفون: Jianwei Li, Haoyuan Deng, Tianyuan Zhao, Yongkang Yang, Runmeng Li, Chao Wang, Hao Li, Qinyu Tian, Ziheng Xu, Tianze Gao, Ruiyang Zhang, Libo Hao, Xiang Sui, Shuyun Liu, Quanyi Guo

المصدر: Materials & Design, Vol 242, Iss , Pp 112990- (2024)

مصطلحات موضوعية: Osteochondral regeneration, Osteochondral interface, Freeze-casting technique, Structure orientation, Biochemical crosstalk, Materials of engineering and construction. Mechanics of materials, TA401-492

الوصف: Despite previous attempts using multiphasic scaffolds to organize cells and bioactive elements, osteochondral repair still faces challenges, such as thin cartilage layers and uneven bone-cartilage interfaces. These issues are likely exacerbated by detrimental biochemical interactions and the formation of new blood vessels within the osteochondral unit, impacting tissue repair quality. In this study, we devised a novel freeze-welding technique to create a unified porous scaffold. Chitosan was chosen as the primary material due to its structural and compositional similarity to glycosaminoglycans in the ECM. In particular, by meticulously adjusting freezing and solute conditions, we engineered the scaffold to have an axially aligned porous structure in the cartilage layer and a radially aligned porous structure in the subchondral layer (scaffold A/R), each designed to meet the unique repair needs of the respective tissues. This scaffold showed enhanced mechanical compressibility, shape retention, and interfacial bonding capabilities. The results demonstrated that this unique spatial scaffold structure offers significant advantages over purely axial scaffolds in guiding osteochondral regeneration. These advantages were evident in its ability to independently promote cartilage and bone growth, reduce undesirable bone-cartilage crosstalk, particularly in the context of vessel invasion in the cartilage layer, and optimize the remodeling of the osteochondral interface. This study offers valuable insights into the structural design patterns for osteochondral regeneration, highlighting the potential importance of structural scaffold design in guiding endogenous tissue remodeling during heterogeneous tissue regeneration.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S0264127524003642Test; https://doaj.org/toc/0264-1275Test

الوصول الحر: https://doaj.org/article/20805f7fbfbf47b69c39d8fb848c5d9bTest

المؤلفون: Liqing Peng, Hao Li, Haoyuan Deng, Tianze Gao, Runmeng Li, Ziheng Xu, Qinyu Tian, Tianyuan Zhao, Jianwei Li, Yongkang Yang, Chao Wang, Shuyun Liu, Quanyi Guo

المصدر: Journal of Orthopaedic Translation, Vol 44, Iss , Pp 72-87 (2024)

مصطلحات موضوعية: Articular cartilage repair, Human articular cartilage-derived extracellular matrix, Microfracture, Sheep, Subchondral bone, Diseases of the musculoskeletal system, RC925-935

الوصف: Background: The utilization of decellularized extracellular matrix has gained considerable attention across numerous areas in regenerative research. Of particular interest is the human articular cartilage-derived extracellular matrix (hACECM), which presents as a promising facilitator for cartilage regeneration. Concurrently, the microfracture (MF) ​technique, a well-established marrow stimulation method, has proven efficacious in the repair of cartilage defects. However, as of the current literature review, no investigations have explored the potential of a combined application of hACECM and the microfracture technique in the repair of cartilage defects within a sheep model. Hypothesis: The combination of hACECM scaffold and microfracture will result in improved repair of full-thickness femoral condyle articular cartilage defects compared to the use of either technique alone. Study design: Controlled laboratory study. Methods: Full-thickness femoral condyle articular cartilage defect (diameter, 7.0 ​mm; debrided down to the subchondral bone plate) were created in the weight-bearing area of the femoral medial and lateral condyles (n ​= ​24). All of defected sheep were randomly divided into four groups: control, microfracture, hACECM scaffold, and hACECM scaffold ​+ ​microfracture. After 3, 6 and 12 months, the chondral repair was assessed for standardized (semi-) quantitative macroscopic, imaging, histological, immunohistochemical, mechanics, and biochemical analyses in each group. Result: At 3, 6 and 12 months after implantation, the gross view and pathological staining of regenerative tissues were better in the hACECM scaffold and hACECM scaffold ​+ ​microfracture groups than in the microfracture and control groups; Micro-CT result showed that the parameters about the calcified layer of cartilage and subchondral bone were better in the hACECM scaffold and hACECM scaffold ​+ ​microfracture groups than the others, and excessive subchondral bone proliferation in the microfracture group. The results demonstrate that human cartilage extracellular matrix scaffold alone is an efficient, safe and simple way to repair cartilage defects. Conclusion: hACECM scaffolds combined with/without microfracture facilitate chondral defect repair. The translational potential of this article: Preclinical large animal models represent an important adjunct and surrogate for studies on articular cartilage repair, while the sheep stifle joint reflects many key features of the human knee and are therefore optimal experimental model for future clinical application in human. In this study, we developed a human articular cartilage-derived extracellular matrix scaffold and to verify the viability of its use in sheep animal models. Clinical studies are warranted to further quantify the effects of hACECM scaffolds in similar settings.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2214031X23000657Test; https://doaj.org/toc/2214-031XTest

الوصول الحر: https://doaj.org/article/70aca3f093a749feb35f6d3e8913411aTest

المؤلفون: Tianyuan Zhao, Xu Li, Hao Li, Haoyuan Deng, Jianwei Li, Zhen Yang, Songlin He, Shuangpeng Jiang, Xiang Sui, Quanyi Guo, Shuyun Liu

المصدر: Acta Pharmaceutica Sinica B, Vol 13, Iss 10, Pp 4127-4148 (2023)

مصطلحات موضوعية: Drug delivery systems, Articular cartilage, Cartilage injury, Osteoarthritis, Cartilage regeneration, Multiple drug delivery strategy, Therapeutics. Pharmacology, RM1-950

الوصف: Articular cartilage (AC) injuries often lead to cartilage degeneration and may ultimately result in osteoarthritis (OA) due to the limited self-repair ability. To date, numerous intra-articular delivery systems carrying various therapeutic agents have been developed to improve therapeutic localization and retention, optimize controlled drug release profiles and target different pathological processes. Due to the complex and multifactorial characteristics of cartilage injury pathology and heterogeneity of the cartilage structure deposited within a dense matrix, delivery systems loaded with a single therapeutic agent are hindered from reaching multiple targets in a spatiotemporal matched manner and thus fail to mimic the natural processes of biosynthesis, compromising the goal of full cartilage regeneration. Emerging evidence highlights the importance of sequential delivery strategies targeting multiple pathological processes. In this review, we first summarize the current status and progress achieved in single-drug delivery strategies for the treatment of AC diseases. Subsequently, we focus mainly on advances in multiple drug delivery applications, including sequential release formulations targeting various pathological processes, synergistic targeting of the same pathological process, the spatial distribution in multiple tissues, and heterogeneous regeneration. We hope that this review will inspire the rational design of intra-articular drug delivery systems (DDSs) in the future.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2211383522004889Test; https://doaj.org/toc/2211-3835Test

الوصول الحر: https://doaj.org/article/400e244796284ab6b13234a467defda2Test

المؤلفون: Haoyuan Deng, Xuehui Zhang, Nan Cheng, Jianghui Zhang, Chongwei Song, Yunrui Sun, Zhongxin Hou, Yi Li, Qian Wang, Jianzhong Yin, Qiong Meng

المصدر: BMC Public Health, Vol 23, Iss 1, Pp 1-11 (2023)

مصطلحات موضوعية: Nephrolithiasis, Serum uric acid, Cross-sectional study, Restricted cubic splines, Public aspects of medicine, RA1-1270

الوصف: Abstract Background Existing evidence shows that there is an independent correlation between nephrolithiasis and gout, and hyperuricemia is the most important risk factor for gout. However, hyperuricemia was often used as an accompanying symptom of gout to explore its association with nephrolithiasis, there were few studies to explore whether hyperuricemia itself or serum uric acid (SUA) is related to the risk of nephrolithiasis. Evidence on the relationship between hyperuricemia and nephrolithiasis is still insufficient. Methods A total of 22,303 participants aged 30 to 79 years who participated in the China Multi-Ethnic Cohort (CMEC) study in Yunnan Province from May 2018 to September 2019 were included in the study. All participants received standardized face-to-face interviews, medical examinations, and biochemical examinations. Logistic regression was used to estimate the association between hyperuricemia and nephrolithiasis, and a restricted cubic spline (RCS) model was used to explore the dose–response relationship between SUA and the risk of nephrolithiasis. Results 14.5% of all participants were diagnosed with hyperuricemia, and 12.1% were diagnosed with nephrolithiasis. After adjusting for all potential confounders, the OR (95%CI) for nephrolithiasis in participants with hyperuricemia compared with participants without hyperuricemia was 1.464 (1.312,1.633), p

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2458Test

الوصول الحر: https://doaj.org/article/0eddea7b43144529a1e035902fa9dbfbTest

المؤلفون: Qian Wang, Yi Li, Jianzhong Yin, Qiong Meng, Yunrui Sun, Xiaoyu Han, Zhongxin Hou, Haoyuan Deng, Nan Cheng, Nishang Zhang, Jianghui Zhang

المصدر: BMJ Open, Vol 13, Iss 12 (2023)

مصطلحات موضوعية: Medicine

الوصف: Objective Sedentary behaviour is associated with a variety of adverse health outcomes, including obesity, oestrogen metabolism and chronic inflammation, all of which are related to the pathogenesis of uterine fibroids (UFs). This study aimed to explore the relationship between leisure sedentary time (LST) and UFs.Design Cross-sectional.Setting We conducted a cross-sectional analysis of data from patients from the Yunnan region in the China Multi-Ethnic Cohort Study.Participants A total of 6623 non-menopausal women aged 30–55 years old were recruited. Menstrual status was self-reported. Participants who lacked a unique national identity card, suffered from serious mental illness, did not have a clear diagnosis of UFs, or provided incomplete information were excluded.Primary and secondary outcome UFs were diagnosed by abdominal B-ultrasound. Leisure sedentary behaviour was assessed by using a face-to-face questionnaire interview. Logistic regression and restricted cubic spline were employed to explore the relationship between LST and UFs.Results A total of 562 participants had UFs, with a prevalence rate of 8.5% (7.8%, 9.2%). Multivariate adjusted logistic regression analysis showed that the risk of UFs in women with LST≥6 hour/day was 2.008 times that in women with LST0.05). According to the results of the stratified analysis for menstrual status and body mass index (BMI), there was a correlation between LST and the prevalence of UFs only in women with a BMI

وصف الملف: electronic resource

العلاقة: https://bmjopen.bmj.com/content/13/12/e073592.fullTest; https://doaj.org/toc/2044-6055Test

الوصول الحر: https://doaj.org/article/ea5cad33dfa74e77b73b7ae47d7dabc6Test

المؤلفون: Haoyang Zhang, Maohuan Ran, Liping Jiang, Xiance Sun, Tianming Qiu, Jing Li, Ningning Wang, Xiaofeng Yao, Cong Zhang, Haoyuan Deng, Shaopeng Wang, Guang Yang

المصدر: Ecotoxicology and Environmental Safety, Vol 268, Iss , Pp 115711- (2023)

مصطلحات موضوعية: DEHP, Apoptosis, PPARα, Mitochondrial dysfunction, Endoplasmic reticulum stress, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350

الوصف: Di-2-ethylhexyl phthalate (DEHP), as a common endocrine disrupting chemicals, can induce toxicity to reproductive system. However, the mechanism remains to be explored. In our study, DEHP exposure induced testicular injury in rats. The high throughput transcriptional sequencing was performed to identify differentially expressed genes (DEGs) between the treatment and control groups. KEGG analysis revealed that DEGs were enriched in apoptosis, PPARα, and ER stress pathway. DEHP up-regulated the expression of PPARα, Bax, Bim, caspase-4. GRP78, PERK, p-PERK, eIF2α, p-eIF2α, ATF4 and CHOP. This view has also been confirmed in TM3 and TM4 cells. In vitro, after pre-treatment with GW6471 (an inhibitor of PPARα) or GSK (an inhibitor of PERK), the apoptosis was inhibited and mitochondrial dysfunction was improved. Moreover, the improvement of mitochondrial dysfunction decreased the expression of PERK pathway by using SS-31(a protective agent for mitochondrial function). Interestingly, ER stress promoted the accumulation of ROS by ERO1L (the downstream of CHOP during ER stress), and the ROS further aggravated the ER stress, thus forming a feedback loop during the apoptosis. In this process, a vicious cycle consisting of PERK, eIF2α, ATF4, CHOP, ERO1L, ROS was involved. Taken together, our results suggested that mitochondrial dysfunction and ER stress-ROS feedback loop caused by PPARα activation played a crucial role in DEHP-induced apoptosis. This work provides insight into the mechanism of DEHP-induced reproductive toxicity.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S0147651323012150Test; https://doaj.org/toc/0147-6513Test

الوصول الحر: https://doaj.org/article/017247dc12eb463f9a68507e91a4ca14Test

المؤلفون: Zhen Yang, Jianwei Li, Haoyuan Deng, Hao Li, Tianyuan Zhao, Tianze Gao, Dan Xing, Jianhao Lin

المصدر: Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)

مصطلحات موضوعية: 3D printing, cartilage regeneration, cartilage repair, tissue engineering, visualization research, Biotechnology, TP248.13-248.65

الوصف: The self-repair ability of cartilage defects is limited, and 3D printing technology provides hope for the repair and regeneration of cartilage defects. Although 3D printing technology and cartilage repair and regeneration have been studied for decades, there are still few articles specifically describing the relationship between 3D printing and cartilage defect repair and regeneration, and a bibliometric analysis has not been completed. To supplement, sort out and summarize the content in related fields, we analyzed the research status of 3D printing technology and cartilage repair and regeneration from 2002 to 2022. According to the set search strategy, the Web of Science Core Collection was used as the data source, and the literature search was completed on December 6, 2022. CiteSpace V and VOSviewer were used as bibliometric tools to complete the analysis of the research focus and direction of the published literature. Based on the analysis results, we focus on the occurrence and development of this field of combined medical and engineering research. Moreover, the current advantages and limitations of this field as well as future development prospects are discussed in depth. It will help to shape researchers’ understanding of 3D printing and cartilage repair and regeneration, inspire researchers’ research ideas, guide research directions, and promote related research results to clinical application.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fbioe.2023.1214715/fullTest; https://doaj.org/toc/2296-4185Test

الوصول الحر: https://doaj.org/article/d52e70435c544815b64e69dd7aa12724Test

المؤلفون: Zineng Yan, Han Yin, Jiang Wu, Guangzhao Tian, Muzhe Li, Zhiyao Liao, Songlin He, Haoyuan Deng, Chao Ning, Zhengang Ding, Xun Yuan, Xiang Sui, Mingxue Chen, Shuyun Liu, Quanyi Guo

المصدر: Materials Today Bio, Vol 19, Iss , Pp 100549- (2023)

مصطلحات موضوعية: Three-dimensional scaffold culture, Exosomes, Cartilage repair, miRNA, Medicine (General), R5-920, Biology (General), QH301-705.5

الوصف: Improving the poor microenvironment in the joint cavity has potential for treating cartilage injury, and mesenchymal stem cell (MSC)-derived exosomes (MSC-Exos), which can modulate cellular behavior, are becoming a new cell-free therapy for cartilage repair. Here, we used acellular cartilage extracellular matrix (ACECM) to prepare 3D scaffolds and 2D substrates by low-temperature deposition modeling (LDM) and tape casting. We aimed to investigate whether MSC-Exos cultured on scaffolds of different dimensions could improve the poor joint cavity microenvironment caused by cartilage injury and to explore the related mechanisms. In vitro experiments showed that exosomes derived from MSCs cultured on three-dimensional (3D) scaffolds (3D-Exos) had increased efficiency. In short-term animal experiments, compared with exosomes derived from MSCs cultured in a two-dimensional (2D) environment (2D-Exos), 3D-Exos had a stronger ability to regulate the joint cavity microenvironment. Long-term animal studies confirmed the therapeutic efficacy of 3D-Exos over 2D-Exos. Thus, 3D-Exos were applied in the rat knee osteochondral defect model after adsorption in the micropores of the scaffold and combined with subsequent articular cavity injections, and they showed a stronger cartilage repair ability. These findings provide a new strategy for repairing articular cartilage damage. Furthermore, miRNA sequencing indicated that the function of 3D-Exos may be associated with high expression of miRNAs. Thus, our study provides valuable insights for the design of 3D-Exos to promote cartilage regeneration.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2590006423000091Test; https://doaj.org/toc/2590-0064Test

الوصول الحر: https://doaj.org/article/0450dbd9ed364e948bfc9efc295be2fdTest

المؤلفون: Haoyuan Deng, Zhuoran Yi, Qiang Shen, Mei Qin, Yi Sun, Guoqiang Luo

المصدر: Materials & Design, Vol 223, Iss , Pp 111103- (2022)

مصطلحات موضوعية: Metastable intermixed composites, PVDF/Al composites, Graphene, Energy release, Combustion, Materials of engineering and construction. Mechanics of materials, TA401-492

الوصف: Energetic metastable intermixed composites (MICs) consisting of polyvinylidene fluoride (PVDF) and fuels aluminum (Al) have the potential to be used as propellants and explosives. Our study introduced SPEEK-modified graphene (SPG) prepared by non-covalent modification of sulfonated poly(ether-ether-ketone) (SPEEK) into PVDF/Al to improve its energy release. The optimized interface generated by the interaction between SO3H groups of SPG layer and CF2 dipoles of PVDF layer integrates graphene onto the surface of PVDF/Al particles. Due to the effect of graphene on the interfacial thermal resistance, the enthalpy of SPG coated PVDF/Al particles is about 1.5 times higher than that of uncoated particles. The existence of SPG improves the heat transfer efficiency of MICs. For comparison, reduced graphene oxide (RGO) has also been introduced as an additive. Graphene is introduced to linearly affect(R2 = 0.9865) the main oxidation enthalpy by lifting the surface oxidation heat release of the Al core. Therefore, graphene is a new strategy for increasing the energy release rate while improving the energy output.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S0264127522007250Test; https://doaj.org/toc/0264-1275Test

الوصول الحر: https://doaj.org/article/2ee8e09a47594cc2af63731dbb2c5c1fTest