المؤلفون: Ramos, Stephan A., Armitage, Lucas H., Morton, John J., Alzofon, Nathaniel, Handler, Diana, Kelly, Geoffrey, Homann, Dirk, Jimeno, Antonio, Russ, Holger A.

المصدر: Stem Cell Reports ; volume 18, issue 4, page 829-840 ; ISSN 2213-6711

الإتاحة: https://doi.org/10.1016/j.stemcr.2023.02.013Test
https://api.elsevier.com/content/article/PII:S2213671123000607?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2213671123000607?httpAccept=text/plainTest

المؤلفون: van der Heide, Verena, Jangra, Sonia, Cohen, Phillip, Rathnasinghe, Raveen, Aslam, Sadaf, Aydillo, Teresa, Geanon, Daniel, Handler, Diana, Kelley, Geoffrey, Lee, Brian, Rahman, Adeeb, Dawson, Travis, Qi, Jingjing, D'Souza, Darwin, Kim-Schulze, Seunghee, Panzer, Julia K., Caicedo, Alejandro, Kusmartseva, Irina, Posgai, Amanda L., Atkinson, Mark A., Albrecht, Randy A., García-Sastre, Adolfo, Rosenberg, Brad R., Schotsaert, Michael, Homann, Dirk

المساهمون: JPB Foundation, Juvenile Diabetes Research Foundation United States of America, National Institutes of Health, Open Philanthropy Project, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases, Defense Advanced Research Projects Agency

المصدر: Cell Reports ; volume 38, issue 11, page 110508 ; ISSN 2211-1247

مصطلحات موضوعية: General Biochemistry, Genetics and Molecular Biology

الإتاحة: https://doi.org/10.1016/j.celrep.2022.110508Test
https://api.elsevier.com/content/article/PII:S2211124722002443?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S2211124722002443?httpAccept=text/plainTest

المؤلفون: Handler, Diana Z.,, Nitecki, Matthew H.

الوصول الحر: https://www.europeana.eu/item/08711/https___www_biodiversitylibrary_org_item_25172?utm_source=api&utm_medium=api&utm_campaign=YuvuWBeCaTest

المؤلفون: Handler, Diana Z.,, Nitecki, Matthew H.

الوصول الحر: https://www.europeana.eu/item/08711/bibliography_5239?utm_source=api&utm_medium=api&utm_campaign=YuvuWBeCaTest

المؤلفون: Geanon, Daniel, Lee, Brian, Gonzalez‐Kozlova, Edgar, Kelly, Geoffrey, Handler, Diana, Upadhyaya, Bhaskar, Leech, John, De Real, Ronaldo M., Herbinet, Manon, Magen, Assaf, Del Valle, Diane, Charney, Alexander, Kim‐Schulze, Seunghee, Gnjatic, Sacha, Merad, Miriam, Rahman, Adeeb H.

المساهمون: National Cancer Institute, National Institute of Allergy and Infectious Diseases

المصدر: Cytometry Part A ; volume 99, issue 5, page 446-461 ; ISSN 1552-4922 1552-4930

الوصف: Mass cytometry (CyTOF) represents one of the most powerful tools in immune phenotyping, allowing high throughput quantification of over 40 parameters at single‐cell resolution. However, wide deployment of CyTOF‐based immune phenotyping studies are limited by complex experimental workflows and the need for specialized CyTOF equipment and technical expertise. Furthermore, differences in cell isolation and enrichment protocols, antibody reagent preparation, sample staining, and data acquisition protocols can all introduce technical variation that can confound integrative analyses of large data‐sets of samples processed across multiple labs. Here, we present a streamlined whole blood CyTOF workflow which addresses many of these sources of experimental variation and facilitates wider adoption of CyTOF immune monitoring across sites with limited technical expertise or sample‐processing resources or equipment. Our workflow utilizes commercially available reagents including the Fluidigm MaxPar Direct Immune Profiling Assay (MDIPA), a dry tube 30‐marker immunophenotyping panel, and SmartTube Proteomic Stabilizer, which allows for simple and reliable fixation and cryopreservation of whole blood samples. We validate a workflow that allows for streamlined staining of whole blood samples with minimal processing requirements or expertise at the site of sample collection, followed by shipment to a central CyTOF core facility for batched downstream processing and data acquisition. We apply this workflow to characterize 184 whole blood samples collected longitudinally from a cohort of 72 hospitalized COVID‐19 patients and healthy controls, highlighting dynamic disease‐associated changes in circulating immune cell frequency and phenotype.

الإتاحة: https://doi.org/10.1002/cyto.a.24317Test

المؤلفون: Charney, Alexander W., Simons, Nicole W., Mouskas, Konstantinos, Lepow, Lauren, Cheng, Esther, Le Berichel, Jessica, Chang, Christie, Marvin, Robert, Del Valle, Diane Marie, Calorossi, Sharlene, Lansky, Alona, Walker, Laura, Patel, Manishkumar, Xie, Hui, Yi, Nancy, Yu, Alex, Kang, Gurpawan, Mendoza, Anthony, Liharska, Lora E., Moya, Emily, Hartnett, Matthew, Hatem, Sandra, Wilkins, Lillian, Eaton, Melody, Jamal, Hajra, Tuballes, Kevin, Chen, Steven T., Tabachnikova, Alexandra, Chung, Jonathan, Harris, Jocelyn, Batchelor, Craig, Lacunza, Jose, Yishak, Mahlet, Argueta, Kimberly, Karekar, Neha, Lee, Brian, Kelly, Geoffrey, Geanon, Daniel, Handler, Diana, Leech, John, Stefanos, Hiyab, Dawson, Travis, Scott, Ieisha, Francoeur, Nancy, Johnson, Jessica S., Vaid, Akhil, Glicksberg, Benjamin S., Nadkarni, Girish N., Schadt, Eric E., Gelb, Bruce D.

المصدر: Nature Medicine ; volume 27, issue 3, page 560-560 ; ISSN 1078-8956 1546-170X

مصطلحات موضوعية: General Biochemistry, Genetics and Molecular Biology, General Medicine

الإتاحة: https://doi.org/10.1038/s41591-021-01247-3Test
http://www.nature.com/articles/s41591-021-01247-3.pdfTest
http://www.nature.com/articles/s41591-021-01247-3Test

المؤلفون: Charney, Alexander W., Simons, Nicole W., Mouskas, Konstantinos, Lepow, Lauren, Cheng, Esther, Le Berichel, Jessica, Chang, Christie, Marvin, Robert, Del Valle, Diane Marie, Calorossi, Sharlene, Lansky, Alona, Walker, Laura, Patel, Manishkumar, Xie, Hui, Yi, Nancy, Yu, Alex, Kang, Gurpawan, Mendoza, Anthony, Liharska, Lora E., Moya, Emily, Hartnett, Matthew, Hatem, Sandra, Wilkins, Lillian, Eaton, Melody, Jamal, Hajra, Tuballes, Kevin, Chen, Steven T., Tabachnikova, Alexandra, Chung, Jonathan, Harris, Jocelyn, Batchelor, Craig, Lacunza, Jose, Yishak, Mahlet, Argueta, Kimberly, Karekar, Neha, Lee, Brian, Kelly, Geoffrey, Geanon, Daniel, Handler, Diana, Leech, John, Stefanos, Hiyab, Dawson, Travis, Scott, Ieisha, Francoeur, Nancy, Johnson, Jessica S., Vaid, Akhil, Glicksberg, Benjamin S., Nadkarni, Girish N., Schadt, Eric E., Gelb, Bruce D.

المصدر: Nature Medicine ; volume 26, issue 8, page 1157-1158 ; ISSN 1078-8956 1546-170X

مصطلحات موضوعية: General Biochemistry, Genetics and Molecular Biology, General Medicine

الإتاحة: https://doi.org/10.1038/s41591-020-1004-3Test
http://www.nature.com/articles/s41591-020-1004-3.pdfTest
http://www.nature.com/articles/s41591-020-1004-3Test

المؤلفون: Charney, Alexander W., Simons, Nicole W., Mouskas, Konstantinos, Lepow, Lauren, Cheng, Esther, Le Berichel, Jessica, Chang, Christie, Marvin, Robert, Del Valle, Diane Marie, Calorossi, Sharlene, Lansky, Alona, Walker, Laura, Patel, Manishkumar, Xie, Hui, Yi, Nancy, Yu, Alex, Kang, Gurpawan, Liharska, Lora E., Moya, Emily, Hartnett, Matthew, Hatem, Sandra, Wilkins, Lillian, Eaton, Melody, Jamal, Hajra, Tuballes, Kevin, Chen, Steven T., Chung, Jonathan, Harris, Jocelyn, Batchelor, Craig, Lacunza, Jose, Yishak, Mahlet, Argueta, Kimberly, Karekar, Neha, Lee, Brian, Kelly, Geoffrey, Geanon, Daniel, Handler, Diana, Leech, John, Stefanos, Hiyab, Dawson, Travis, Scott, Ieisha, Francoeur, Nancy, Johnson, Jessica S., Vaid, Akhil, Glicksberg, Benjamin S., Nadkarni, Girish N., Schadt, Eric E., Gelb, Bruce D., Rahman, Adeeb, Sebra, Robert

المصدر: Nature Medicine ; volume 26, issue 9, page 1493-1493 ; ISSN 1078-8956 1546-170X

مصطلحات موضوعية: General Biochemistry, Genetics and Molecular Biology, General Medicine

الإتاحة: https://doi.org/10.1038/s41591-020-1057-3Test
https://www.nature.com/articles/s41591-020-1057-3.pdfTest
https://www.nature.com/articles/s41591-020-1057-3Test

المؤلفون: van der Heide, Verena, Jangra, Sonia, Cohen, Phillip, Rathnasinghe, Raveen, Aslam, Sadaf, Aydillo, Teresa, Geanon, Daniel, Handler, Diana, Kelly, Geoffrey, Lee, Brian, Rahman, Adeeb, Dawson, Travis, Qui, Jingjin, D'Souza, Darwin, Kim-Schulze, Seunghee, Panzer, Julia, Caicedo, Alejandro, Kusmartseva, Irina, Posgai, Amanda, Atkinson, Mark A., Albrecht, Randy, Garcia-Sastre, Adolfo, Rosenberg, Brad, Schotsaert, Michael, Homann, Dirk

المصدر: SSRN Electronic Journal ; ISSN 1556-5068

مصطلحات موضوعية: Industrial and Manufacturing Engineering, Polymers and Plastics, History, Business and International Management

الإتاحة: https://doi.org/10.2139/ssrn.3945929Test

المؤلفون: Nitecki, Matthew H., Handler, Diana Z.

المساهمون: University Library, University of Illinois Urbana Champaign

مصطلحات موضوعية: Catalogs and collections, North America, Ostracoda, Fossil, Paleontology, Paleozoic

الوصول الحر: http://www.biodiversitylibrary.org/bibliography/5239Test