المؤلفون: Kiemen, Ashley L., Forjaz, André, Sousa, Ricardo, Han, Kyu Sang, Hruban, Ralph H., Wood, Laura D., Wu, PeiHsun, Wirtz, Denis

المساهمون: Rolfe Pancreatic Cancer Foundation, Lustgarten Foundation, National Cancer Institute, Troper Wojcicki Foundation

المصدر: Advanced Materials Technologies ; volume 9, issue 6 ; ISSN 2365-709X 2365-709X

الوصف: Pancreatic ductal adenocarcinoma (PDAC) is a deadly cancer that can develop from pancreatic intraepithelial neoplasia (PanIN), a microscopic lesion in the pancreatic ductal system. PanIN and PDAC are conventionally studied in 2D via histological tissue sections. As such, their true structure is poorly understood due to the inability to image them in 3D. CODA, a recently developed technique for reconstruction of tissues at cellular resolution, is used to study the 3D morphology of the pancreas. Here, CODA is extended through 3D printing of normal pancreatic ducts, PanIN, and PDAC at cm‐scale and µm resolution. A methodology is presented to create 3D printable files from anatomical maps generated from serial histological images and to show detailed validation of the accuracy of this method. Existing 3D printing workflows utilizing medical images derived from computerized tomography (CT), X‐ray, and magnetic resonance imaging (MRI) are scientifically proven to be useful in printing whole organ‐scale structures with sub‐mm resolution. Here, using serial histological sections, it is demonstrated that 3D printing of higher‐resolution structures is also possible. Finally, with the 3D models of normal ducts, PanIN, and PDAC, marked changes to the structure of the human pancreas during tumorigenesis are revealed.

الإتاحة: https://doi.org/10.1002/admt.202301837Test

المؤلفون: Jain, Sanjay, Pei, Liming, Spraggins, Jeffrey M., Angelo, Michael, Carson, James P., Gehlenborg, Nils, Ginty, Fiona, Gonçalves, Joana P., Hagood, James S., Hickey, John W., Kelleher, Neil L., Laurent, Louise C., Lin, Shin, Lin, Yiing, Liu, Huiping, Naba, Alexandra, Nakayasu, Ernesto S., Qian, Wei-Jun, Radtke, Andrea, Robson, Paul, Stockwell, Brent R., Van de Plas, Raf, Vlachos, Ioannis S., Zhou, Mowei, Ahn, Kyung Jin, Allen, Jamie, Anderson, David M., Anderton, Christopher R., Curcio, Christine, Angelin, Alessia, Arvanitis, Constadina, Atta, Lyla, Awosika-Olumo, Demi, Bahmani, Amir, Bai, Huajun, Balderrama, Karol, Balzano, Leandro, Bandyopadhyay, Gautam, Bandyopadhyay, Shovik, Bar-Joseph, Ziv, Barnhart, Kurt, Barwinska, Daria, Becich, Michael, Becker, Laren, Becker, Winston, Bedi, Kenneth, Bendall, Sean, Benninger, Kathy, Betancur, David, Bettinger, Keith, Billings, Sunteasja, Blood, Philip, Bolin, Daniel, Border, Samuel, Bosse, Marc, Bramer, Lisa, Brewer, Maya, Brusko, Maigan, Bueckle, Andreas, Burke, Karl, Burnum-Johnson, Kristin, Butcher, Eugene, Butterworth, Elizabeth, Cai, Long, Calandrelli, Riccardo, Caldwell, Michael, Campbell-Thompson, Martha, Cao, Dongfeng, Cao-Berg, Ivan, Caprioli, Richard, Caraccio, Chiara, Caron, Anita, Carroll, Megan, Chadwick, Chrystal, Chen, Angela, Chen, Derek, Chen, Fei, Chen, Haoran, Chen, Jing, Chen, Li, Chen, Lu, Chiacchia, Kenneth, Cho, Sanghee, Chou, Peter, Choy, Lisa, Cisar, Cecilia, Clair, Geremy, Clarke, Laura, Clouthier, Kelly A., Colley, Madeline E., Conlon, Kristin, Conroy, John, Contrepois, Kevin, Corbett, Anthony, Corwin, Alex, Cotter, Daniel, Courtois, Elise, Cruz, Aaron, Csonka, Christopher, Czupil, Kimberley, Daiya, Vicky, Dale, Kali, Davanagere, Shakeel Ahamed, Dayao, Monica, de Caestecker, Mark P., Decker, Aubrianna, Deems, Stephen, Degnan, David, Desai, Tushar, Deshpande, Vikrant, Deutsch, Gail, Devlin, Michelle, Diep, Dinh, Dodd, Carla, Donahue, Sean, Dong, Weixiu, dos Santos Peixoto, Rafael, Duffy, Michael, Dufresne, Martin, Duong, Thu Elizabeth, Dutra, Jennifer, Eadon, Michael T., El-Achkar, Tarek M., Enninful, Archibald, Eraslan, Gokcen, Eshelman, Diane, Espin-Perez, Almudena, Esplin, Edward D., Esselman, Allison, Falo, Louis D., Falo, Louis, Fan, Jean, Fan, Rong, Farrow, Melissa A., Farzad, Negin, Favaro, Patricia, Fermin, Jamie, Filiz, Ferda, Filus, Shane, Fisch, Kathleen, Fisher, Eyal, Fisher, Stephen, Flowers, Katelyn, Flynn, William F., Fogo, Agnes B., Fu, Dongtao, Fulcher, James, Fung, Anthony, Furst, Derek, Gallant, Michael, Gao, Fu, Gao, Yu, Gaulton, Kyle, Gaut, Joseph P., Gee, James, Ghag, Reetika R., Ghazanfar, Shila, Ghose, Soumya, Gisch, Debora, Gold, Ilan, Gondalia, Aashay, Gorman, Brittney, Greenleaf, William, Greenwald, Noah, Gregory, Brian, Guo, Rong, Gupta, Rajat, Hakimian, Hunter, Haltom, Jeff, Halushka, Marc, Han, Kyu Sang, Hanson, Casey, Harbury, Pehr, Hardi, Josef, Harlan, Linda, Harris, Raymond C., Hartman, Austin, Heidari, Elyas, Helfer, Jesse, Helminiak, David, Hemberg, Martin, Henning, Nathaniel, Herr, Bruce W., Ho, Jonhan, Holden-Wiltse, Jeanne, Hong, Seung-Hyun, Hong, Young-Kwon, Honick, Brendan, Hood, Greg, Hu, Po, Hu, Qiwen, Huang, Molly, Huyck, Heidie, Imtiaz, Tamjid, Isberg, Olof Gerdur, Itkin, Maxim, Jackson, Dana, Jacobs, Marni, Jain, Yashvardhan, Jewell, David, Jiang, Lihua, Jiang, Zhenghui G., Johnston, Sarah, Joshi, Pujan, Ju, Yingnan, Judd, Audra, Kagel, Adam, Kahn, Ari, Kalavros, Nikolaos, Kalhor, Kian, Karagkouni, Dimitra, Karathanos, Thomas, Karunamurthy, Arivarasan, Katari, Suhas, Kates, Heather, Kaushal, Madhurima, Keener, Nicholas, Keller, Mark, Kenney, Mariah, Kern, Colin, Kharchenko, Peter, Kim, Junhyong, Kingsford, Carl, Kirwan, Jessica, Kiselev, Vladimir, Kishi, Jocelyn, Kitata, Reta Birhanu, Knoten, Amanda, Kollar, Charles, Krishnamoorthy, Praveen, Kruse, Angela R. S., Kuang, Da, Kundaje, Anshul, Kutschera, Eric, Kwon, Yumi, Lake, Blue B., Lancaster, Samuel, Langlieb, Jonah, Lardenoije, Roy, Laronda, Monica, Laskin, Julia, Lau, Ken, Lee, Hayan, Lee, Maria, Lee, Mejeong, Levites Strekalova, Yulia, Li, Dongshunyi, Li, Jennifer, Li, Jilong, Li, Xiangtang, Li, Zhi, Liao, Yen-Chen, Liaw, Tiffany, Lin, Pei, Lin, Yulieh, Lindsay, Scott, Liu, Chunjie, Liu, Yang, Liu, Yuan, Lott, Marie, Lotz, Martin, Lowery, Lisa, Lu, Peiran, Lu, Xinyue, Lucarelli, Nicholas, Lun, Xiaokang, Luo, Zhifei, Ma, Jian, Macosko, Evan, Mahajan, Mayank, Maier, Libby, Makowski, Danika, Malek, Morad, Manthey, David, Manz, Trevor, Margulies, Kenneth, Marioni, John, Martindale, Matthew, Mason, Cayla, Mathews, Clayton, Maye, Peter, McCallum, Chuck, McDonough, Elizabeth, McDonough, Liz, Mcdowell, Hannah, Meads, Morgan, Medina-Serpas, Miguel, Melo Ferreira, Ricardo, Messinger, Jeffrey, Metis, Kay, Migas, Lukasz G., Miller, Brendan, Mimar, Sayat, Minor, Brittany, Misra, Ravi, Missarova, Alsu, Mistretta, Christopher, Moens, Roger, Moerth, Eric, Moffitt, Jeffrey, Molla, Gesmira, Monroe, Matthew, Monte, Emma, Morgan, Mike, Muraro, Daniele, Murphy, Bob, Murray, Evan, Musen, Mark A., Naglah, Ahmed, Nasamran, Chanond, Neelakantan, Taruna, Nevins, Stephanie, Nguyen, Hieu, Nguyen, Nam, Nguyen, Tram, Nguyen, Tri, Nigra, Deb, Nofal, Michel, Nolan, Garry, Nwanne, Gerald, O'Connor, Martin, Okuda, Kenichi, Olmer, Merissa, O'Neill, Kathleen, Otaluka, Nancy, Pang, Minxing, Parast, Mana, Pasa-Tolic, Ljiljana, Paten, Benedict, Patterson, Nathan Heath, Peng, Ting, Phillips, Gesina, Pichavant, Mina, Piehowski, Paul, Pilner, Hannah, Pingry, Ellie, Pita-Juarez, Yered, Plevritis, Sylvia, Ploumakis, Athanasios, Pouch, Alison, Pryhuber, Gloria, Puerto, Juan, Qaurooni, Danial, Qin, Ling, Quardokus, Ellen M., Rajbhandari, Presha, Rakow-Penner, Rebecca, Ramasamy, Ramalakshmi, Read, David, Record, Elizabeth G., Reeves, David, Ricarte, Allyson, Rodríguez-Soto, Ana, Ropelewski, Alexander, Rosario, Jean, Roselkis, Morla-Adames, Rowe, David, Roy, Tarun Kanti, Ruffalo, Matt, Ruschman, Nancy, Sabo, Angela, Sachdev, Nina, Saka, Sinem, Salamon, Diane, Sarder, Pinaki, Sasaki, Hiroshi, Satija, Rahul, Saunders, Diane, Sawka, Riley, Schey, Kevin, Schlehlein, Heidi, Scholten, David, Schultz, Sarah, Schwartz, Lauren, Schwenk, Melissa, Scibek, Robin, Segre, Ayellet, Serrata, Matthew, Shands, Walter, Shen, Xiaotao, Shendure, Jay, Shephard, Holly, Shi, Lingyan, Shi, Tujin, Shin, Dong-Guk, Shirey, Bill, Sibilla, Max, Silber, Michal, Silverstein, Jonathan, Simmel, Derek, Simmons, Alan, Singhal, Dhruv, Sivajothi, Santhosh, Smits, Thomas, Soncin, Francesca, Song, Qi, Stanley, Valentina, Stuart, Tim, Su, Hanquan, Su, Pei, Sun, Xin, Surrette, Christine, Swahn, Hannah, Tan, Kai, Teichmann, Sarah, Tejomay, Abhiroop, Tellides, George, Thomas, Kathleen, Thomas, Tracey, Thompson, Marissa, Tian, Hua, Tideman, Leonoor, Trapnell, Cole, Tsai, Albert G., Tsai, Chia-Feng, Tsai, Leo, Tsui, Elizabeth, Tsui, Tina, Tung, Jason, Turner, Morgan, Uranic, Jackie, Vaishnav, Eeshit Dhaval, Varra, Sricharan Reddy, Vaskivskyi, Vasyl, Velickovic, Dusan, Velickovic, Marija, Verheyden, Jamie, Waldrip, Jessica, Wallace, Douglas, Wan, Xueyi, Wang, Allen, Wang, Fusheng, Wang, Meng, Wang, Shuoshuo, Wang, Xuefei, Wasserfall, Clive, Wayne, Leonard, Webber, James, Weber, Griffin M., Wei, Bei, Wei, Jian-Jun, Weimer, Annika, Welling, Joel, Wen, Xingzhao, Wen, Zishen, Williams, MacKenzie, Winfree, Seth, Winograd, Nicholas, Woodard, Abashai, Wright, Devin, Wu, Fan, Wu, Pei-Hsun, Wu, Qiuyang, Wu, Xiaodong, Xing, Yi, Xu, Tiangyang, Yang, Manxi, Yang, Mingyu, Yap, Joseph, Ye, Dong Hye, Yin, Peng, Yuan, Zhou, Yun, Chi (Jina), Zahraei, Ali, Zemaitis, Kevin, Zhang, Bo, Zhang, Caibin, Zhang, Chenyu, Zhang, Chi, Zhang, Kun, Zhang, Shiping, Zhang, Ted, Zhang, Yida, Zhao, Bingqing, Zhao, Wenxin, Zheng, Jia Wen, Zhong, Sheng, Zhu, Bokai, Zhu, Chenchen, Zhu, Diming, Zhu, Quan, Zhu, Ying, Börner, Katy, Snyder, Michael P., HuBMAP Consortium

المصدر: Nature Cell Biology, 26, (2024-03-01)

مصطلحات موضوعية: Cell Biology

الوصف: The Human BioMolecular Atlas Program (HuBMAP) aims to create a multi-scale spatial atlas of the healthy human body at single-cell resolution by applying advanced technologies and disseminating resources to the community. As the HuBMAP moves past its first phase, creating ontologies, protocols and pipelines, this Perspective introduces the production phase: the generation of reference spatial maps of functional tissue units across many organs from diverse populations and the creation of mapping tools and infrastructure to advance biomedical research. ; © Springer Nature Limited 2023. ; Correction to: Nature Cell Biology https://doi.org/10.1038/s41556-023-01194-wTest. Published online 19 July 2023. In the version of this article originally published, the name of Tianyang Xu was misspelled as Tiangyang Xu. The name has been corrected in the HTML and PDF versions of the article. ; The HuBMAP Consortium GitHub athttps://github.com/hubmapconsortiumhas 120 repositories that support data ingest, analysis, visualization and search plus HRA construction and usage. Documentation is available athttps://software.docs.hubmapconsortium.org/apis.html. ; We thank L. M. McGuire, SciStories, F. Goncalves, H. Schlehlein and V. A. Deshpande for their efforts in designing and creating graphics. We thank A. Honkala for assistance with manuscript formatting. We thank Z. Galis from the National Heart, Lung, and Blood Institute for many useful comments. Support for title page creation and format was provided by AuthorArranger, a tool developed at the National Cancer Institute. The authors gratefully acknowledge NIH HuBMAP grants U54HL165442 and U01HL166058 (L.P.); U54DK134301 and OT2OD033753 (S.J.); U54EY032442, U54DK120058 and U54DK134302 (J.M.S.); OT2OD033756 and OT2OD026671 (K.B.); UH3CA246635 (N.L.K.); UG3CA256967 (H.L.); U54HG010426 (M.P.S.); UH3CA246633 (M.A.); U54HD104393 (L.C.L. and P.R.); U54DK127823 (E.S.N., J.P.C. and W.-J.Q.); OT2OD033758 (N.G.); UH3CA246594 (F.G.); U54EY032442 and U54DK134302 (J.P.G.); U54HL145611 (S.L. and ...

العلاقة: https://authors.library.caltech.edu/records/44139-r9855Test; https://doi.org/10.1038/s41556-024-01384-0Test; oai:authors.library.caltech.edu:r1dag-ysc77

الإتاحة: https://doi.org/10.1038/s41556-024-01384-0Test

المؤلفون: Jain, Sanjay, Pei, Liming, Spraggins, Jeffrey M., Angelo, Michael, Carson, James P., Gehlenborg, Nils, Ginty, Fiona, Gonçalves, Joana P., Hagood, James S., Hickey, John W., Kelleher, Neil L., Laurent, Louise C., Lin, Shin, Lin, Yiing, Liu, Huiping, Naba, Alexandra, Nakayasu, Ernesto S., Qian, Wei-Jun, Radtke, Andrea, Robson, Paul, Stockwell, Brent R., Van de Plas, Raf, Vlachos, Ioannis S., Zhou, Mowei, Ahn, Kyung Jin, Allen, Jamie, Anderson, David M., Anderton, Christopher R., Curcio, Christine, Angelin, Alessia, Arvanitis, Constadina, Atta, Lyla, Awosika-Olumo, Demi, Bahmani, Amir, Bai, Huajun, Balderrama, Karol, Balzano, Leandro, Bandyopadhyay, Gautam, Bandyopadhyay, Shovik, Bar-Joseph, Ziv, Barnhart, Kurt, Barwinska, Daria, Becich, Michael, Becker, Laren, Becker, Winston, Bedi, Kenneth, Bendall, Sean, Benninger, Kathy, Betancur, David, Bettinger, Keith, Billings, Sunteasja, Blood, Philip, Bolin, Daniel, Border, Samuel, Bosse, Marc, Bramer, Lisa, Brewer, Maya, Brusko, Maigan, Bueckle, Andreas, Burke, Karl, Burnum-Johnson, Kristin, Butcher, Eugene, Butterworth, Elizabeth, Cai, Long, Calandrelli, Riccardo, Caldwell, Michael, Campbell-Thompson, Martha, Cao, Dongfeng, Cao-Berg, Ivan, Caprioli, Richard, Caraccio, Chiara, Caron, Anita, Carroll, Megan, Chadwick, Chrystal, Chen, Angela, Chen, Derek, Chen, Fei, Chen, Haoran, Chen, Jing, Chen, Li, Chen, Lu, Chiacchia, Kenneth, Cho, Sanghee, Chou, Peter, Choy, Lisa, Cisar, Cecilia, Clair, Geremy, Clarke, Laura, Clouthier, Kelly A., Colley, Madeline E., Conlon, Kristin, Conroy, John, Contrepois, Kevin, Corbett, Anthony, Corwin, Alex, Cotter, Daniel, Courtois, Elise, Cruz, Aaron, Csonka, Christopher, Czupil, Kimberley, Daiya, Vicky, Dale, Kali, Davanagere, Shakeel Ahamed, Dayao, Monica, de Caestecker, Mark P., Decker, Aubrianna, Deems, Stephen, Degnan, David, Desai, Tushar, Deshpande, Vikrant, Deutsch, Gail, Devlin, Michelle, Diep, Dinh, Dodd, Carla, Donahue, Sean, Dong, Weixiu, dos Santos Peixoto, Rafael, Duffy, Michael, Dufresne, Martin, Duong, Thu Elizabeth, Dutra, Jennifer, Eadon, Michael T., El-Achkar, Tarek M., Enninful, Archibald, Eraslan, Gokcen, Eshelman, Diane, Espin-Perez, Almudena, Esplin, Edward D., Esselman, Allison, Falo, Louis D., Falo, Louis, Fan, Jean, Fan, Rong, Farrow, Melissa A., Farzad, Negin, Favaro, Patricia, Fermin, Jamie, Filiz, Ferda, Filus, Shane, Fisch, Kathleen, Fisher, Eyal, Fisher, Stephen, Flowers, Katelyn, Flynn, William F., Fogo, Agnes B., Fu, Dongtao, Fulcher, James, Fung, Anthony, Furst, Derek, Gallant, Michael, Gao, Fu, Gao, Yu, Gaulton, Kyle, Gaut, Joseph P., Gee, James, Ghag, Reetika R., Ghazanfar, Shila, Ghose, Soumya, Gisch, Debora, Gold, Ilan, Gondalia, Aashay, Gorman, Brittney, Greenleaf, William, Greenwald, Noah, Gregory, Brian, Guo, Rong, Gupta, Rajat, Hakimian, Hunter, Haltom, Jeff, Halushka, Marc, Han, Kyu Sang, Hanson, Casey, Harbury, Pehr, Hardi, Josef, Harlan, Linda, Harris, Raymond C., Hartman, Austin, Heidari, Elyas, Helfer, Jesse, Helminiak, David, Hemberg, Martin, Henning, Nathaniel, Herr, Bruce W., Ho, Jonhan, Holden-Wiltse, Jeanne, Hong, Seung-Hyun, Hong, Young-Kwon, Honick, Brendan, Hood, Greg, Hu, Po, Hu, Qiwen, Huang, Molly, Huyck, Heidie, Imtiaz, Tamjid, Isberg, Olof Gerdur, Itkin, Maxim, Jackson, Dana, Jacobs, Marni, Jain, Yashvardhan, Jewell, David, Jiang, Lihua, Jiang, Zhenghui G., Johnston, Sarah, Joshi, Pujan, Ju, Yingnan, Judd, Audra, Kagel, Adam, Kahn, Ari, Kalavros, Nikolaos, Kalhor, Kian, Karagkouni, Dimitra, Karathanos, Thomas, Karunamurthy, Arivarasan, Katari, Suhas, Kates, Heather, Kaushal, Madhurima, Keener, Nicholas, Keller, Mark, Kenney, Mariah, Kern, Colin, Kharchenko, Peter, Kim, Junhyong, Kingsford, Carl, Kirwan, Jessica, Kiselev, Vladimir, Kishi, Jocelyn, Kitata, Reta Birhanu, Knoten, Amanda, Kollar, Charles, Krishnamoorthy, Praveen, Kruse, Angela R. S., Kuang, Da, Kundaje, Anshul, Kutschera, Eric, Kwon, Yumi, Lake, Blue B., Lancaster, Samuel, Langlieb, Jonah, Lardenoije, Roy, Laronda, Monica, Laskin, Julia, Lau, Ken, Lee, Hayan, Lee, Maria, Lee, Mejeong, Levites Strekalova, Yulia, Li, Dongshunyi, Li, Jennifer, Li, Jilong, Li, Xiangtang, Li, Zhi, Liao, Yen-Chen, Liaw, Tiffany, Lin, Pei, Lin, Yulieh, Lindsay, Scott, Liu, Chunjie, Liu, Yang, Liu, Yuan, Lott, Marie, Lotz, Martin, Lowery, Lisa, Lu, Peiran, Lu, Xinyue, Lucarelli, Nicholas, Lun, Xiaokang, Luo, Zhifei, Ma, Jian, Macosko, Evan, Mahajan, Mayank, Maier, Libby, Makowski, Danika, Malek, Morad, Manthey, David, Manz, Trevor, Margulies, Kenneth, Marioni, John, Martindale, Matthew, Mason, Cayla, Mathews, Clayton, Maye, Peter, McCallum, Chuck, McDonough, Elizabeth, McDonough, Liz, Mcdowell, Hannah, Meads, Morgan, Medina-Serpas, Miguel, Melo Ferreira, Ricardo, Messinger, Jeffrey, Metis, Kay, Migas, Lukasz G., Miller, Brendan, Mimar, Sayat, Minor, Brittany, Misra, Ravi, Missarova, Alsu, Mistretta, Christopher, Moens, Roger, Moerth, Eric, Moffitt, Jeffrey, Molla, Gesmira, Monroe, Matthew, Monte, Emma, Morgan, Mike, Muraro, Daniele, Murphy, Bob, Murray, Evan, Musen, Mark A., Naglah, Ahmed, Nasamran, Chanond, Neelakantan, Taruna, Nevins, Stephanie, Nguyen, Hieu, Nguyen, Nam, Nguyen, Tram, Nguyen, Tri, Nigra, Deb, Nofal, Michel, Nolan, Garry, Nwanne, Gerald, O'Connor, Martin, Okuda, Kenichi, Olmer, Merissa, O'Neill, Kathleen, Otaluka, Nancy, Pang, Minxing, Parast, Mana, Pasa-Tolic, Ljiljana, Paten, Benedict, Patterson, Nathan Heath, Peng, Ting, Phillips, Gesina, Pichavant, Mina, Piehowski, Paul, Pilner, Hannah, Pingry, Ellie, Pita-Juarez, Yered, Plevritis, Sylvia, Ploumakis, Athanasios, Pouch, Alison, Pryhuber, Gloria, Puerto, Juan, Qaurooni, Danial, Qin, Ling, Quardokus, Ellen M., Rajbhandari, Presha, Rakow-Penner, Rebecca, Ramasamy, Ramalakshmi, Read, David, Record, Elizabeth G., Reeves, David, Ricarte, Allyson, Rodríguez-Soto, Ana, Ropelewski, Alexander, Rosario, Jean, Roselkis, Morla-Adames, Rowe, David, Roy, Tarun Kanti, Ruffalo, Matt, Ruschman, Nancy, Sabo, Angela, Sachdev, Nina, Saka, Sinem, Salamon, Diane, Sarder, Pinaki, Sasaki, Hiroshi, Satija, Rahul, Saunders, Diane, Sawka, Riley, Schey, Kevin, Schlehlein, Heidi, Scholten, David, Schultz, Sarah, Schwartz, Lauren, Schwenk, Melissa, Scibek, Robin, Segre, Ayellet, Serrata, Matthew, Shands, Walter, Shen, Xiaotao, Shendure, Jay, Shephard, Holly, Shi, Lingyan, Shi, Tujin, Shin, Dong-Guk, Shirey, Bill, Sibilla, Max, Silber, Michal, Silverstein, Jonathan, Simmel, Derek, Simmons, Alan, Singhal, Dhruv, Sivajothi, Santhosh, Smits, Thomas, Soncin, Francesca, Song, Qi, Stanley, Valentina, Stuart, Tim, Su, Hanquan, Su, Pei, Sun, Xin, Surrette, Christine, Swahn, Hannah, Tan, Kai, Teichmann, Sarah, Tejomay, Abhiroop, Tellides, George, Thomas, Kathleen, Thomas, Tracey, Thompson, Marissa, Tian, Hua, Tideman, Leonoor, Trapnell, Cole, Tsai, Albert G., Tsai, Chia-Feng, Tsai, Leo, Tsui, Elizabeth, Tsui, Tina, Tung, Jason, Turner, Morgan, Uranic, Jackie, Vaishnav, Eeshit Dhaval, Varra, Sricharan Reddy, Vaskivskyi, Vasyl, Velickovic, Dusan, Velickovic, Marija, Verheyden, Jamie, Waldrip, Jessica, Wallace, Douglas, Wan, Xueyi, Wang, Allen, Wang, Fusheng, Wang, Meng, Wang, Shuoshuo, Wang, Xuefei, Wasserfall, Clive, Wayne, Leonard, Webber, James, Weber, Griffin M., Wei, Bei, Wei, Jian-Jun, Weimer, Annika, Welling, Joel, Wen, Xingzhao, Wen, Zishen, Williams, MacKenzie, Winfree, Seth, Winograd, Nicholas, Woodard, Abashai, Wright, Devin, Wu, Fan, Wu, Pei-Hsun, Wu, Qiuyang, Wu, Xiaodong, Xing, Yi, Xu, Tiangyang, Yang, Manxi, Yang, Mingyu, Yap, Joseph, Ye, Dong Hye, Yin, Peng, Yuan, Zhou, Yun, Chi, Zahraei, Ali, Zemaitis, Kevin, Zhang, Bo, Zhang, Caibin, Zhang, Chenyu, Zhang, Chi, Zhang, Kun, Zhang, Shiping, Zhang, Ted, Zhang, Yida, Zhao, Bingqing, Zhao, Wenxin, Zheng, Jia Wen, Zhong, Sheng, Zhu, Bokai, Zhu, Chenchen, Zhu, Diming, Zhu, Quan, Zhu, Ying, Börner, Katy, Snyder, Michael P., HuBMAP Consortium

المصدر: Nature Cell Biology, 25(8), 1089-1100, (2023-08)

مصطلحات موضوعية: Cell Biology, envir, geo

الوصف: The Human BioMolecular Atlas Program (HuBMAP) aims to create a multi-scale spatial atlas of the healthy human body at single-cell resolution by applying advanced technologies and disseminating resources to the community. As the HuBMAP moves past its first phase, creating ontologies, protocols and pipelines, this Perspective introduces the production phase: the generation of reference spatial maps of functional tissue units across many organs from diverse populations and the creation of mapping tools and infrastructure to advance biomedical research. ; © Springer Nature Limited 2023. We thank L. M. McGuire, SciStories, F. Goncalves, H. Schlehlein and V. A. Deshpande for their efforts in designing and creating graphics. We thank A. Honkala for assistance with manuscript formatting. We thank Z. Galis from the National Heart, Lung, and Blood Institute for many useful comments. Support for title page creation and format was provided by AuthorArranger, a tool developed at the National Cancer Institute. The authors gratefully acknowledge NIH HuBMAP grants U54HL165442 and U01HL166058 (L.P.); U54DK134301 and OT2OD033753 (S.J.); U54EY032442, U54DK120058 and U54DK134302 (J.M.S.); OT2OD033756 and OT2OD026671 (K.B.); UH3CA246635 (N.L.K.); UG3CA256967 (H.L.); U54HG010426 (M.P.S.); UH3CA246633 (M.A.); U54HD104393 (L.C.L. and P.R.); U54DK127823 (E.S.N., J.P.C. and W.-J.Q.); OT2OD033758 (N.G.); UH3CA246594 (F.G.); U54EY032442 and U54DK134302 (J.P.G.); U54HL145611 (S.L. and Y.L.); U01HG012680 (A.N.); U54DK120058, U54EY032442 and U54DK134302 (B.R.S.); UG3CA256959 (M.Z.); and U54HL165440 (I.S.V.). The authors are also supported by these grants: 2U01DK114933, P50DK133943 and U24DK135157 (S.J.); U54HL145608 and U54HL165443 (J.S.H.); U24CA268108 and U2CDK114886 (K.B.); Department of Defense W81XWH-22-1-0058 and Additional Ventures (L.P.). J.W.H. was supported by an NIH T32 Fellowship (T32CA196585) and an American Cancer Society: Roaring Fork Valley Postdoctoral Fellowship (PF-20-032-01-CSM). This work was supported, in part, by the .

العلاقة: https://doi.org/10.1038/s41556-023-01194-wTest

الإتاحة: https://doi.org/10.1038/s41556-023-01194-wTest

المؤلفون: Kiemen, Ashley L., Braxton, Alicia M., Grahn, Mia P., Han, Kyu Sang, Babu, Jaanvi Mahesh, Reichel, Rebecca, Jiang, Ann C., Kim, Bridgette, Hsu, Jocelyn, Amoa, Falone, Reddy, Sashank, Hong, Seung-Mo, Cornish, Toby C., Thompson, Elizabeth D., Huang, Peng, Wood, Laura D., Hruban, Ralph H., Wirtz, Denis, Wu, Pei-Hsun

المساهمون: Foundation for the National Institutes of Health, The Sol Goldman Pancreatic Cancer Research Center, ARCS Foundation, Inc, Susan Wojcicki and Dennis Troper, The Sol Goldman Pancreatic Cancer Research Center, The Sol Goldman Pancreatic Cancer Research Center, Susan Wojcicki and Dennis Troper, The Rolfe Foundation for Pancreatic Cancer Research, Allegheny Health Network – Johns Hopkins Cancer Research Fund, The Sol Goldman Pancreatic Cancer Research Center, Susan Wojcicki and Dennis Troper, The Sol Goldman Pancreatic Cancer Research Center, NVIDIA GPU Grant

المصدر: Nature Methods ; volume 19, issue 11, page 1490-1499 ; ISSN 1548-7091 1548-7105

مصطلحات موضوعية: Cell Biology, Molecular Biology, Biochemistry, Biotechnology

الإتاحة: https://doi.org/10.1038/s41592-022-01650-9Test
https://www.nature.com/articles/s41592-022-01650-9.pdfTest
https://www.nature.com/articles/s41592-022-01650-9Test

مستخلص: An artificial nail is formed by injection molding a first section and a second section. The first section and second section are adjacent to one another. One of the sections defines a front distal tip of the artificial nail and is formed by injecting a quantity of heated material under pressure from a nozzle into a sprue, through a runner and through a cavity gate into a mold cavity. The other section is formed by injecting another quantity of heated material under pressure from a heated nozzle through another cavity gate into the mold cavity.

المؤلفون: Phillip, Jude M., Han, Kyu-Sang, Chen, Wei-Chiang, Wirtz, Denis, Wu, Pei-Hsun

المساهمون: U.S. Department of Health & Human Services | NIH | National Cancer Institute, U.S. Department of Health & Human Services | NIH | National Institute on Aging

المصدر: Nature Protocols ; volume 16, issue 2, page 754-774 ; ISSN 1754-2189 1750-2799

مصطلحات موضوعية: General Biochemistry, Genetics and Molecular Biology

الإتاحة: https://doi.org/10.1038/s41596-020-00432-xTest
https://www.nature.com/articles/s41596-020-00432-x.pdfTest
https://www.nature.com/articles/s41596-020-00432-xTest

مستخلص: An artificial nail or tip arrangement and method of making the same are provided. The arrangement includes a body having a concave lower surface with a shape corresponding to a natural nail. The arrangement also includes an adhesive layer having a first surface and an opposing second surface. The first surface adheres to the concave lower surface of the body and the second surface is provided to adhere to the natural nail when applied thereto. The arrangement further includes a removable layer that covers the second surface of the adhesive layer, and which is removable to expose the second surface of the adhesive layer for application to the natural nail. The removable layer is provided with a plurality of slits so that a surface of the removable layer remains smooth after adherence of the first surface of the adhesive layer with the concave lower surface of the body.

مستخلص: An ultrasonic wave energy artificial nail remover in one aspect includes a handle, a body attached to the handle, an ultrasonic sound wave generator attached to the body, and a tip having the shape of a natural nail. The tip is made to vibrate by the ultrasonic sound wave generator. The shape of the tip allows for a quicker removal of the artificial nail and requires less skill to successfully remove an artificial nail without damaging the natural nail than removal with a narrow flat tip allows and requires. Ultrasonic energy is channeled through the natural nail shaped tip to facilitate removal of an artificial nail in a quick manner with little cleanup involved.

مستخلص: An artificial nail is formed by injection molding a first section and a second section. The first section and second section are adjacent to one another. One of the sections defines a front distal tip of the artificial nail and is formed by injecting a quantity of heated material under pressure from a nozzle into a sprue, through a runner and through a cavity gate into a mold cavity. The other section is formed by injecting another quantity of heated material under pressure from a heated nozzle through another cavity gate into the mold cavity.

مستخلص: Exemplary embodiments of artificial nail or tip arrangement and method of making the same can be provided. For example, at least one body can be provided which can have a particular surface with a shape that at least approximately corresponds to a shape of at least one portion of a natural nail. Further, an adhesive layer can be secured to at least one portion of the surface of the body. The adhesive layer can have a first surface which adheres to at least one portion of the particular surface of the body and a second surface which is provided to adhere to an upper surface of the at least one portion of the natural nail when directly applied thereto. Further, a removable layer can be provided which covers at least one section of the second surface of the adhesive layer, and which is removable to expose at least one portion of the second surface of the adhesive layer for an application to the upper surface of the portion of the natural nail. The removable layer can include at least one section which extends outwardly outside a periphery of the body.