المؤلفون: Bláhová, Lucie, Nováková, Zuzana, Večeřa, Zbyněk, Vrlíková, Lucie, Dočekal, Bohumil, Dumková, Jana, Křůmal, Kamil, Mikuška, Pavel, Buchtová, Marcela, Hampl, Aleš, Hilscherová, Klára, Bláha, Luděk

المصدر: Nanotoxicology; Mar2020, Vol. 14 Issue 2, p214-231, 18p

مصطلحات موضوعية: DNA damage, GENETIC markers, MEMBRANE lipids, OLFACTORY nerve, TRANSMISSION electron microscopy

مستخلص: Although the production of engineered nanoparticles increases our knowledge of toxicity and mechanisms of bioactivity during relevant exposures is lacking. In the present study mice were exposed to PbO nanoparticles (PbONP; 192.5 µg/m³; 1.93 × 10⁶ particles/cm³) for 2, 5 and 13 weeks through continuous inhalation. The analyses addressed Pb and PbONP distribution in organs (lung, liver, kidney, brain) using electrothermal atomic absorption spectrometry and transmission electron microscopy, as well as histopathology and analyses of oxidative stress biomarkers. New LC-MS/MS methods were validated for biomarkers of lipid damage F2-isoprostanes (8-iso-prostaglandins F2-alpha and E2) and hydroxylated deoxoguanosine (8-OHdG, marker of DNA oxidation). Commonly studied malondialdehyde was also measured as TBARS by HPLC-DAD. The study revealed fast blood transport and distribution of Pb from the lung to the kidney and liver. A different Pb accumulation trend was observed in the brain, suggesting transfer of NP along the nasal nerve to the olfactory bulbs. Long-term inhalation of PbONP caused lipid peroxidation in animal brains (increased levels of TBARS and both isoprostanes). Membrane lipid damage was also detected in the kidney after shorter exposures, but not in the liver or lung. On the contrary, longer exposures to PbONP increased levels of 8-OHdG in the lung and temporarily increased lung weight after 2 and 5 weeks of exposure. The histopathological changes observed mainly in the lung and liver indicated inflammation and general toxicity responses. The present long-term inhalation study indicates risks of PbONP to both human health and the environment. [ABSTRACT FROM AUTHOR]

: Copyright of Nanotoxicology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Kučíre, Martin, Bagherpoor, Alireza J., Jaroš, Josef, Hampl, Aleš, Štros, Michal

المصدر: FASEB Journal; Dec2019, Vol. 33 Issue 12, p14307-14324, 18p

المؤلفون: Posplšilova, Veronika, Ešner, Milan, Cervenkova, Iveta, Fedr, Radek, Tinevez, Jean-Yvez, Hampl, Aleš, Anger, Martin

المصدر: Journal of Applied Biomedicine; 2019, Vol. 17 Issue 4, p209-217, 9p

مصطلحات موضوعية: MITOSIS, EMBRYONIC stem cells, MICROSCOPY, CELLULAR therapy

مستخلص: Embryonic stem (ES) cells are pluripotent cells widely used in cell therapy and tissue engineering. However, the broader clinical applications of ES cells are limited by their genomic instability and karyotypic abnormalities. Thus, understanding the mechanisms underlying ES cell karyotypic abnormalities is critical to optimizing their clinical use. In this study, we focused on proliferating human and mouse ES cells undergoing multipolar divisions. Specifically, we analyzed the frequency and outcomes of such divisions using a combination of time-lapse microscopy and cell tracking. This revealed that cells resulting from multipolar divisions were not only viable, but they also frequently underwent subsequent cell divisions. Our novel data also showed that in human and mouse ES cells, multipolar spindles allowed more robust escape from chromosome segregation control mechanisms than bipolar spindles. Considering the frequency of multipolar divisions in proliferating ES cells, it is conceivable that cell division errors underlie ES cell karyotypic instability. [ABSTRACT FROM AUTHOR]

: Copyright of Journal of Applied Biomedicine is the property of University of South Bohemia in Ceske Budejovice, Faculty of Health & Social Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

المؤلفون: Kaucka, Marketa, Zikmund, Tomas, Tesarova, Marketa, Gyllborg, Daniel, Hellander, Andreas, Jaros, Josef, Kaiser, Jozef, Petersen, Julian, Szarowska, Bara, Newton, Phillip T, Dyachuk, Vyacheslav, Li, Lei, Qian, Hong, Johansson, Anne-Sofie, Mishina, Yuji, Currie, Joshua D, Tanaka, Elly M, Erickson, Alek, Dudley, Andrew, Brismar, Hjalmar, Southam, Paul, Coen, Enrico, Chen, Min, Weinstein, Lee S, Hampl, Ales, Arenas, Ernest, Chagin, Andrei S, Fried, Kaj, Adameyko, Igor

الإتاحة: https://doi.org/10.7554/elife.25902.028Test

المؤلفون: Skoda, Jan, Nunukova, Alena, Loja, Tomas, Zambo, Iva, Neradil, Jakub, Mudry, Peter, Zitterbart, Karel, Hermanova, Marketa, Hampl, Ales, Sterba, Jaroslav, Veselska, Renata

المساهمون: Internal Grant Agency of the Czech Ministry of Healthcare, National Program of Sustainability II, European Regional Development Fund

المصدر: Tumor Biology ; volume 37, issue 7, page 9535-9548 ; ISSN 1010-4283 1423-0380

مصطلحات موضوعية: General Medicine

الإتاحة: https://doi.org/10.1007/s13277-016-4837-0Test

المؤلفون: Kratochvílová, Kateřina, Moráň, Lukáš, Paďourová, Stanislava, Stejskal, Stanislav, Tesařová, Lenka, Šimara, Pavel, Hampl, Aleš, Koutná, Irena, Vaňhara, Petr

المساهمون: Ministry of Education, Youth and Sports of the Czech Republic, Grant Agency of the Czech Republic, Masaryk University

المصدر: European Journal of Cell Biology ; volume 95, issue 3-5, page 115-123 ; ISSN 0171-9335

مصطلحات موضوعية: Cell Biology, General Medicine, Histology, Pathology and Forensic Medicine

الإتاحة: https://doi.org/10.1016/j.ejcb.2016.02.002Test
https://api.elsevier.com/content/article/PII:S017193351630005X?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S017193351630005X?httpAccept=text/plainTest

المؤلفون: Švachová, Veronika, Vojtová, Lucy, Pavliňák, David, Vojtek, Libor, Sedláková, Veronika, Hyršl, Pavel, Alberti, Milan, Jaroš, Josef, Hampl, Aleš, Jančář, Josef

المساهمون: Ministry of Education, Youth and Sports of the Czech Republic, European Regional Development Fund

المصدر: Materials Science and Engineering: C ; volume 67, page 493-501 ; ISSN 0928-4931

مصطلحات موضوعية: Biomaterials, Bioengineering, Mechanics of Materials

الإتاحة: https://doi.org/10.1016/j.msec.2016.05.059Test
https://api.elsevier.com/content/article/PII:S0928493116304945?httpAccept=text/xmlTest
https://api.elsevier.com/content/article/PII:S0928493116304945?httpAccept=text/plainTest

المؤلفون: Kolářová, Lenka, Prokeš, Lubomír, Kučera, Lukáš, Hampl, Aleš, Peňa-Méndez, Eladia, Vaňhara, Petr, Havel, Josef

المصدر: Journal of the American Society for Mass Spectrometry ; volume 28, issue 3, page 419-427 ; ISSN 1044-0305

الإتاحة: https://doi.org/10.1007/s13361-016-1567-xTest

المؤلفون: Jirik, Radovan, Soucek, Karel, Mezl, Martin, Bartos, Michal, Drazanova, Eva, Drafi, Frantisek, Grossova, Lucie, Kratochvila, Jiei, Macicek, Ondrej, Nylund, Kim, Hampl, Ales, Gilja, Odd Helge, Taxt, Torfinn, Starcuk, Zenon

المصدر: 2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

الإتاحة: https://doi.org/10.1109/embc.2014.6944569Test
http://xplorestaging.ieee.org/ielx7/6923026/6943513/06944569.pdf?arnumber=6944569Test

المؤلفون: Kolářová, Lenka, Kučera, Lukáš, Vaňhara, Petr, Hampl, Aleš, Havel, Josef

المساهمون: Grant Agency of Masaryk University, HistoPARK - Centre for Analysis and Modeling of Tissues and Organs

المصدر: Rapid Communications in Mass Spectrometry ; volume 29, issue 17, page 1585-1595 ; ISSN 0951-4198 1097-0231

الوصف: Rationale Many kinds of nanoparticles (NPs) have been used for mass spectrometry (MS) so far. Here we report the first use of flower‐like gold nanoparticles (AuNPs) as a mediator to enhance ionization in MS of peptides and proteins. Methods Flower‐like AuNPs were characterized using transmission and scanning electron microscopy, UV‐VIS spectrophotometry, and laser desorption/ionization (LDI)‐MS and compared with polyhedral AuNPs. Mass spectra were obtained in positive ion mode using a time‐of‐flight (TOF) analyzer coupled with either matrix‐assisted laser desorption/ionization (MALDI) or surface‐assisted laser desorption/ionization (SALDI) methods. Results The intensities of peptide peaks ( m/z 500–3500) were up to 7.5× and up to 7× higher using flower‐like AuNPs and flower‐like AuNPs‐enriched α‐cyano‐4‐hydroxycinnamic acid (CHCA) matrix respectively, than the classical CHCA matrix. The signals of higher mass peptide/protein peaks ( m/z 3600–17000) were up to 2× higher with using flower‐like AuNPs‐enriched CHCA matrix than conventional CHCA matrix. The signal of profile peaks generated by intact cell MALDI‐TOFMS of fibroblast suspension ( m/z 4000–20000) was 2× higher with using flower‐like AuNPs combined with sinapinic acid (SA) compared to SA matrix alone. The use of flower‐like AuNPs as internal calibration standard for the calibration of MS spectra of peptides was performed. Conclusions Flower‐like AuNPs and flower‐like AuNPs combined with CHCA or SA as combined matrices for MS measurement of peptides and proteins were used. Comparison of the conventional MALDI method and our method with flower‐like AuNPs was carried out. In addition, gold clusters generated from flower‐like AuNPs by SALDI provide a suitable internal calibration standard for MS analysis of peptides. Copyright © 2015 John Wiley & Sons, Ltd.

الإتاحة: https://doi.org/10.1002/rcm.7265Test