المؤلفون: Hajime KUBO, Hajime OKITA, Junki SOGANO, Masahiro TODA, Mototsugu OYA, Nobuyuki TANAKA, Satoshi TAKAHASHI

المصدر: NMC Case Report Journal. 2023, 10:235

المؤلفون: Hajime KUBO, Katsuhiro MIZUTANI, Masahiro KATSUMATA, Masahiro TODA, Takashi IWAMA, Takenori AKIYAMA

المصدر: NMC Case Report Journal. 2023, 10:125

المؤلفون: Yijun Yang, Jaslyn Johnson, Constantine D. Troupes, Eric A. Feldsott, Lindsay Kraus, Emily Megill, Zilin Bian, Ngefor Asangwe, Tabito Kino, Deborah M. Eaton, Tao Wang, Marcus Wagner, Lena Ma, Christopher Bryan, Markus Wallner, Hajime Kubo, Remus M. Berretta, Mohsin Khan, Hong Wang, Raj Kishore, Steven R. Houser, Sadia Mohsin

المصدر: Redox Biology, Vol 67, Iss , Pp 102909- (2023)

مصطلحات موضوعية: Cortical bone stem cells, Extracellular vesicles, Cardiac repair, Immune modulation, Medicine (General), R5-920, Biology (General), QH301-705.5

الوصف: Few therapies have produced significant improvement in cardiac structure and function after ischemic cardiac injury (ICI). Our possible explanation is activation of local inflammatory responses negatively impact the cardiac repair process following ischemic injury. Factors that can alter immune response, including significantly altered cytokine levels in plasma and polarization of macrophages and T cells towards a pro-reparative phenotype in the myocardium post-MI is a valid strategy for reducing infarct size and damage after myocardial injury.Our previous studies showed that cortical bone stem cells (CBSCs) possess reparative effects after ICI. In our current study, we have identified that the beneficial effects of CBSCs appear to be mediated by miRNA in their extracellular vesicles (CBSC-EV). Our studies showed that CBSC-EV treated animals demonstrated reduced scar size, attenuated structural remodeling, and improved cardiac function versus saline treated animals. These effects were linked to the alteration of immune response, with significantly altered cytokine levels in plasma, and polarization of macrophages and T cells towards a pro-reparative phenotype in the myocardium post-MI. Our detailed in vitro studies demonstrated that CBSC-EV are enriched in miR-182/183 that mediates the pro-reparative polarization and metabolic reprogramming in macrophages, including enhanced OXPHOS rate and reduced ROS, via Ras p21 protein activator 1 (RASA1) axis under Lipopolysaccharides (LPS) stimulation. In summary, CBSC-EV deliver unique molecular cargoes, such as enriched miR-182/183, that modulate the immune response after ICI by regulating macrophage polarization and metabolic reprogramming to enhance repair.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2213231723003105Test; https://doaj.org/toc/2213-2317Test

الوصول الحر: https://doaj.org/article/f68a1e3939344780bdcabe697059e9a3Test

المؤلفون: Hajime KUBO, Hirotsugu NOGAWA, Katsuhiro MIZUTANI, Masahiro TODA, Taichi SAYANAGI, Takenori AKIYAMA, 久保 創, 佐柳 太一, 戸田 正博, 水谷 克洋, 秋山 武紀, 野川 博嗣

المصدر: 脳血管内治療 / No Kekkannai Chiryo. 2023, 8(2):63

المؤلفون: Hajime Kubo, Kazunori Asai, Nobuhiro Inoue, Rie Yamauchi, Shuji Ohno, 久保 元, 井上 信宏, 大野 修司, 山内 理恵, 浅井 和範

المصدر: 薬学教育 / Japanese Journal of Pharmaceutical Education. 2023, 7:2022-044

المؤلفون: Hajime KUBO, Masateru KATAYAMA, Sadao SUGA, Satoshi INOUE, 久保 創, 井上 賢, 片山 正輝, 菅 貞郎

المصدر: 脳卒中の外科 / Surgery for Cerebral Stroke. 2021, 49(1):26

المؤلفون: Hajime Kubo, Masateru Katayama, Sadao Suga, Satoshi Inoue, Utaro Hino, 久保 創, 井上 賢, 日野 宇太郎, 片山 正輝, 菅 貞郎

المصدر: 脳卒中 / Japanese Journal of Stroke. 2021, 43(1):63

المؤلفون: Norihiko Sasaki, Yoko Itakura, Sadia Mohsin, Tomoaki Ishigami, Hajime Kubo, Yumi Chiba

المصدر: International Journal of Molecular Sciences, Vol 22, Iss 21, p 11849 (2021)

مصطلحات موضوعية: cortical bone stem cells, stemness, glycan profile, TGF-β1, Biology (General), QH301-705.5, Chemistry, QD1-999

الوصف: The newly established mouse cortical-bone-derived stem cells (mCBSCs) are unique stem cells compared to mouse mesenchymal stem cells (mMSCs). The mCBSC-treated hearts after myocardial infarction have been reported to have greater improvement in myocardial structure and functions. In this study, we examined the stemness features, cell surface glycan profiles, and paracrine functions of mCBSCs compared with mMSCs. The stemness analysis revealed that the self-renewing capacity of mCBSCs was greater than mMSCs; however, the differentiation capacity of mCBSCs was limited to the chondrogenic lineage among three types of cells (adipocyte, osteoblast, chondrocyte). The cell surface glycan profiles by lectin array analysis revealed that α2-6sialic acid is expressed at very low levels on the cell surface of mCBSCs compared with that on mMSCs. In contrast, the lactosamine (Galβ1-4GlcNAc) structure, poly lactosamine- or poly N-acetylglucosamine structure, and α2-3sialic acid on both N- and O-glycans were more highly expressed in mCBSCs. Moreover, we found that mCBSCs secrete a greater amount of TGF-β1 compared to mMSCs, and that the TGF-β1 contributed to the self-migration of mCBSCs and activation of fibroblasts. Together, these results suggest that unique characteristics in mCBSCs compared to mMSCs may lead to advanced utility of mCBSCs for cardiac and noncardiac repair.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/1422-0067/22/21/11849Test; https://doaj.org/toc/1661-6596Test; https://doaj.org/toc/1422-0067Test

الوصول الحر: https://doaj.org/article/9365f118b0e94f028fe399831c050fbdTest

المؤلفون: Thomas E. Sharp, III, BA, Hajime Kubo, PhD, Remus M. Berretta, BS, Timothy Starosta, BS, Markus Wallner, MD, PhD, Giana J. Schena, BS, Alexander R. Hobby, BS, Daohai Yu, PhD, Danielle M. Trappanese, PhD, Jon C. George, MD, Jeffery D. Molkentin, PhD, Steven R. Houser, PhD

المصدر: JACC: Basic to Translational Science, Vol 2, Iss 6, Pp 669-683 (2017)

مصطلحات موضوعية: acute myocardial infarction, heart failure with reduced ejection fraction, invasive hemodynamics, PKCα/β inhibitor, positive inotropy, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Inotropic support is often required to stabilize the hemodynamics of patients with acute decompensated heart failure; while efficacious, it has a history of leading to lethal arrhythmias and/or exacerbating contractile and energetic insufficiencies. Novel therapeutics that can improve contractility independent of beta-adrenergic and protein kinase A-regulated signaling, should be therapeutically beneficial. This study demonstrates that acute protein kinase C-α/β inhibition, with ruboxistaurin at 3 months’ post-myocardial infarction, significantly increases contractility and reduces the end-diastolic/end-systolic volumes, documenting beneficial remodeling. These data suggest that ruboxistaurin represents a potential novel therapeutic for heart failure patients, as a moderate inotrope or therapeutic, which leads to beneficial ventricular remodeling.

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S2452302X17301559Test; https://doaj.org/toc/2452-302XTest

الوصول الحر: https://doaj.org/article/dd3c3f7a8fed47668b3c56e6c1ad9062Test

المؤلفون: Aya Sasaki, Hajime Kubo, Masateru Katayama, Sadao Suga, Satoshi Inoue

المصدر: Journal of Neuroendovascular Therapy. 2020, 14(2):62