العنوان البديل: تنقيه وتوصيف االسبراجينيز المنتج من .sp Bacillus. (Arabic)

المؤلفون: I. H., Sahar, A. H., Zaid, A., Milad

المصدر: Baghdad Science Journal; 2023 Supplement, Vol. 20, p2342-2354, 13p

مصطلحات موضوعية: BACILLUS (Bacteria), ENZYME stability, GEL permeation chromatography, FRUCTOSE

مصطلحات جغرافية: BAGHDAD (Iraq)

الملخص (بالإنجليزية): The objective of this study was to isolate and identify the asparaginase-producing bacteria, then purify and characterize the enzyme in order to investigate their properties in the future. Fifteen local bacterial isolates were isolated from various sites in the city of Baghdad, identified by conventional morphological and biochemical procedures, and confirmed using vitek 2 methods, and submitted to primary screening processes for asparaginase production. For secondary screening, eight isolates with the greatest yellow zone ability on a specific solid medium were chosen. Bacillus sp. was reported to have the highest enzyme production (7.5 U/mg proteins). After 24 hours of incubation, submerged fermentation yielded optimal conditions for the production of L-asparaginase (L-ASNase) by the chosen isolate, with medium (2) serving as the optimal medium for production and fructose serving as the optimal source of carbon. In pH 6 at 40°C, Sephadex G-150 gel filtration chromatography was used to purify the enzyme. The final purification folds were increased by 2.5 times, resulting in an enzyme yield of 93.7%. It also showed the highest purified enzyme activity and stability was at 37°C. Also it revealed the highest activity and stability at pH 7.0 and pH 8.0 respectively. Enzyme lost activity when exposed to several metallic ions at concentrations of 1, 5, and 10 mM. [ABSTRACT FROM AUTHOR]

الملخص (بالعربية): هدفت الدراسة الى عزل وتشخيص البكتيريا المنتجة لألسباراجينيز ، ثم تنقية اإلنزيم وتوصيفه من أجل تحديد خصائصه في المستقبل. تم عزل 15 عزلة بكتيرية محلية من مواقع مختلفة في مدينة بغداد، وتم تشخيصها بوساطة االختبارات المظهريه والكيميائية واختبارات الفيتيك، خضعت العزالت لعمليات الفحص األولي إلنتاج األسباراجينيز؛ حيث تم اختيار ثماني عزالت للفحص الثانوي ذات القابليه االعلى في انتاج الهاله الصفراء في الوسط الصلب . بينت النتائج أن البكتريا العصويه من جنس sp Bacillus. لديها اعلى إنتاجية إنزيمية )7.5 وحدة / ملغم بروتين( بعد 24 ساعة من الحضانة ، حدتت إلى الظروف المثلى إلنتاج االنزيم بواسطة العزلة المختارة عن طريق التخمير المغمور ، حيث اختير الوسط )2( كأفضل وسيط لإلنتاج وبوجود الفركتوز كأفضل مصدر للكربون عند درجة حموضة 6 ودرجه حرارة 40 درجة مئوية. تم تنقيه االنزيم بوساطه كروماتوغرافيا الترشيح الهالمي باستخدام جل السيفادكس –ج 150 ، حيث ازدادت عدد مرات التنقية النهائية بمقدار 2.5 مرة ، مما أدى إلى إنتاج إنزيم بنسبة .٪93.7 كما أظهر االنزيم المنقى أعلى فعاليه وثباتيه عند درجه حرارة 37 م ، و اعطى اعلى فعاليه عند الرقم الهيدروجيني 7.0 واعلى ثباتيه عند الرقم الهيدروجيني .8.0 و فقد اإلنزيم نشاطه عند تعرضه لعدة أيونات معدنية بتركيزات 1 ، 5 ملي مولر. [ABSTRACT FROM AUTHOR]

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المؤلفون: Mohamed Grida, Rehab Mohamed, Abdelnaser H. Zaid

الوصف: The Internet of Things (IoT) is used in Supply chain management (SCM) systems to respond to the globalization of complex and dynamic markets and competitiveness in various supply chain scopes. Despite the current buzz about IoT and its role in the supply chain, there is not enough empirical data or extensive expertise to guide its implementation. Therefore, this paper addresses the ambiguity of the assessing the performance of the IoT based supply chain by integrating plithogenic set with both of the Best-Worst method (BWM) and Vlse Kriterijumska Optimizacija Kompromisno Resenje (VIKOR) methods in a decision-making framework tailored for this field. The framework is based on 23 criteria that measure different aspects of the performance. The performance of the framework is assess according to plithogenic set theory and neutrosophic set theory using a case study of comparing the performance of IoT implantation with the SC of five e-commerce companies using three experts. The case study showed that the proposed framework has more consideration of the contradiction degree of each criteria to improve the accuracy of the evaluation results

العلاقة: https://zenodo.org/record/3808980Test; https://doi.org/10.5281/zenodo.3808980Test; oai:zenodo.org:3808980

الإتاحة: https://doi.org/10.5281/zenodo.3808980Test
https://doi.org/10.5281/zenodo.3808979Test
https://zenodo.org/record/3808980Test

المؤلفون: Wael Elabbassi, Farah Al Aila, Mohammed Andaleeb Chowdhury, Ahmed Najib, H. Zaid, M. Michelin, Arif Al Nooryani

المصدر: Indian Heart Journal, Vol 69, Iss 6, Pp 762-766 (2017)

مصطلحات موضوعية: Cardiogenic shock, Adult respiratory distress syndrome, Extra-corporal oxygenation, SOFA score, Multi-organ failure, Surgery, RD1-811, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Introduction: ECMO provides respiratory and circulatory support in critically ill patients. In our study, we report on a single center experience with ECMO and aim to identify the prognostic markers for survival to discharge from hospital. Methods: A registry was maintained on all patients who underwent ECMO implantation from September 2012 till January 2016 at a single institution. The collected data was analyzed to identify baseline characteristics, outcomes including clinical variables predictive of poor outcome. Results: A total of 29 patients underwent ECMO implantation. The average age of patients was 42 ± 18 years. 59% were males (N = 17). 19 cases had a cardiac indication for ECMO (66%) while 10 cases had a pulmonary indication (34%). On univariate analysis; presence of Multi-organ failure, SOFA score more than 18 and hemoglobin less than 10 g/dl at baseline and after ECMO removal were associated with increased 30 day mortality. Pearson correlation with 30 day mortality showed a positive correlation with MOF (+0.562, p = 0.002) and SOFA score >18 (+0.448, p = 0.015) and a negative correlation with anemia (−0.507, p = 0.005). 15 out of the total 29 patients (52%) died within 30 days of admission. Patients with MOF (log rank: 10.926, p = 0.001), SOFA score >18 (log rank: 7.758, p = 0.005) and hemoglobin

وصف الملف: electronic resource

العلاقة: http://www.sciencedirect.com/science/article/pii/S001948321630462XTest; https://doaj.org/toc/0019-4832Test

الوصول الحر: https://doaj.org/article/dabf8db4dbf446f68efc3ae13c20c57fTest

المؤلفون: Muhammad, Z. H. (Zaid)

المصدر: Jurnal Teknik Mesin Mercu Buana

مصطلحات موضوعية: Pompa, head loss, self cleaning filter, Hazen-William, Darcy-Weisbach, De Chezy-Manning, Indonesia

الوصف: Salah satu faktor penting dalam menentukan pompa yang akan digunakan dalam suatu sistem adalah head. Di PT.XY terdapat pompa water intake yang digunakan untuk mengambil air sungai yang akan diolah melewati self cleaning filter agar turbidity air sebelum masuk ke proses selanjutnya berkurang. Permasalahan yang terjadi pada PT.XY adalah terjadi kehilangan tekanan sebesar 0.7 [bar] dari discharge pompa menuju inlet self cleaning filter karena pengaruh jarak & fitting pada sistem pemipaan. Untuk menyelesaikan masalah tersebut dibutuhkan analisa kebutuhan minimum head pompa water intake dengan tiga metode yang berbeda, yaitu Hazen-William, Darcy-Weisbach, dan De Chezy-Manning. Dari hasil perhitungan dan analisa metode Hazen-William mempunyai error terkecil sebesar 6.2% dan minimum pressure pompa water intake untuk melewati self cleaning filter adalah 4.2 [bar]

وصف الملف: application/pdf

العلاقة: https://www.neliti.com/publications/340350/analisa-head-pompa-water-intake-terhadap-self-cleaning-filter-pada-ptxyTest

الإتاحة: https://www.neliti.com/publications/340350/analisa-head-pompa-water-intake-terhadap-self-cleaning-filter-pada-ptxyTest

العنوان البديل: تعدد اشكال النيوكليوتيدة المفردة لجين CRISP2 وعالقتها بوهن الخصوبة لدى المرضى العراقيين.

المؤلفون: A. H., Zaid¹ zaid.a@sc.uobaghdad.edu.iq, Al-Kazaz, Abdulkareem A.²

المصدر: Iraqi Journal of Agricultural Sciences. 2023, Vol. 54 Issue 3, p657-666. 10p.

مصطلحات موضوعية: *MALE infertility, *GENETIC polymorphisms, *INFERTILITY, *SINGLE nucleotide polymorphisms, *SPERMATOZOA, *GENE frequency, *MORPHOLOGY

الشركة/الكيان: ORGANIZATION of American States

الملخص (بالإنجليزية): The objective of this study was to evaluate the association and effect of CRISP2 variants on the risk of asthenozoospermia, a male infertility condition marked by absent or diminished sperm motility. There are numerous reasons why individuals develop asthenozoospermia. Therefore, it is crucial to understand the molecular mechanisms underlying this condition of infertility. Furthermore, seminal plasma, a rich source of sperm quality-related biomarkers, transports the many spermatozoa pools that make up human ejaculate down the epididymis. These spermatozoa vary in size, shape, and motility. The morphology and mobility of male ejaculated spermatozoa are affected by a number of genes, including CRISP2. In seminal samples from 120 Iraqi infertile male patients and 40 healthy males who were matched for age, gender, and ethnicity as a control group, the connection of the CRISP2 gene single nucleotide polymorphisms L56V, M176I, and C196R with infertility was investigated. According to statistical analysis of the genotype distribution of these three nsSNPs of the CRISP2 gene in patients with the asthenozoospermia subgroup and the control group, there weren't detectable differences in genotype distribution between AS, OAS, OTA, and fertile men in the Iraqi research sample. Based on allele frequencies, C, T, and G were determined to be protective alleles, with OR values of 0.74, 0.64, and 2.0, respectively. [ABSTRACT FROM AUTHOR]

الملخص (بالعربية): تهدف الدراسة الحالية الى تقييم التأثير والعالقه بين تغايرات المورث CRISPs وخطر وهن النطف كحالة من عدم الخصوبة عند الذكور تتميز بانخفاض أو عدم وجود حيوانات منوية حركة، ومسبباتها في البشر عوامل متعددة. وبالتالي ، فإن توضيح العمليات الجزيئية التي تسبب هذه الحالة أمر مهم. يتكون القذف البشري من تجمعات غير متجانسة من الحيوانات المنوية متفاوتة الخصائص مثل الشكل والحجم والحركة التي يتم نقلها عبر ا ً البربخ خالل البالزما المنوية ، والتي تعد مصدر ا ً غني للعالمات الحيوية المتعلقة بجودة الحيوانات المنوية. يعد 2CRISP واحًدا من عدة جينات تساهم في شكل وحركة الحيوانات المنوية الذكرية المقذوفة. في الدراسة تم التحقق من العالقة بين تعدد أشكال النوكليوتيدات المفردة V56L و I176M و R196C للمورث 2CRISP مع وهن الخصوبة في عينة السائل المنوي المأخوذة من ًض 120 ا من الذكور الع ارقيين المصابين و مري 40 من الذكور األصحاء المتطابقين في العمر والجنس والعرق كمجموعة ضابطة. أظهرت نتائج التحليل االحصائي لتوزيع الطرز الوراثية لهذه األشكال الثالثة غير المترادفة للمورث 2CRISP في المجموعات الفرعية للمرضى بوهن النطف ومجموعة السيطرة عدم وجود اختالفات ذات داللة احصائية في توزيع هذه االنماط بين المجاميع الفرعية المختلفة والرجال العراقيين الخصبين في عينة الدراسة. وأظهرت نتائج تحليل تكرار األليالت C و T و G كأليالت واقية بقيم النسبة الفردية 0.74 و 0.64 و 2.0على التوالي. [ABSTRACT FROM AUTHOR]

المؤلفون: Ahmed G. Osman Ahmed Khalil, Mohamed H. Zaid Karim Fahmy

المصدر: The Medical Journal of Cairo University. 89:1745-1751

مصطلحات موضوعية: Laparoscopic sleeve gastrectomy, medicine.medical_specialty, business.industry, medicine.medical_treatment, medicine.disease, University hospital, Omentopexy, Group B, Surgery, Stenosis, Staple line, Medicine, Operative time, business, Prospective cohort study

الوصف: Background: Laparoscopic sleeve gastrecomy is the most popular bariatric procedure with good outcome and acceptable complications (bleeding, leakage, stenosis or twist). Aim of Study: To evaluate the role of omentopexy rein-forcement of staple line in reducing postoperative leakage, bleeding and gastric twist. Patients and Methods: This is a prospective cohort study conducted at Ain-Shams University Hospitals, in the period From May 2017 to November 2020. Four hundred morbid obese patients were recruited for this study. Ethical approval was obtained from the Ethical Committee of Ain Shams University Hospitals, and informed written consents were obtained from all patients. Results: Our study included 200 patients who underwent Laparoscopic sleeve gastrectomy, one hundred patients un-derwent LSG without omentopexty (group A) and the other one hundred patients underwent LSG with omentopexy (group B). As regards the age, group A (control group) mean age of 35.74±7.88 while group B (omentopexy group) mean age of 35.6±7.85. The mean BMI 40.95±5.25 in group A while 40.94±5.02 in group B. Three cases of group A (1.5%) while group B had no cases of postoperative leakage with p-value is 0.24. Two cases of group A had postoperative bleeding (1%) while it did not happen in group B with P value is 0.499. Gastric twist had happened in only 1 case of group A (0.5%) while did not happen in any cases of group B, P value is 1. The mean operative time in group A 46.08±10.95 minutes, while 67.49±9.19 minutes in group B with p-value 0.00. Conclusion: LSG with omentopexy reduce rate of post-operative complications (bleeding, leakage and gastric twist) but need longer time than conventional LSG.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::2fdb8f16658e6292597df1ecf812ae59Test
https://doi.org/10.21608/mjcu.2021.197249Test

المؤلفون: Mohamed H. Zaid, Fawzy Salah Fawzy Ahmed Khalil

المصدر: The Medical Journal of Cairo University. 89:1739-1744

مصطلحات موضوعية: medicine.medical_specialty, Axillary lymph nodes, business.industry, Breast surgery, medicine.medical_treatment, Ultrasound, Modified Radical Mastectomy, medicine.disease, Breast cancer, medicine.anatomical_structure, Lymphedema, medicine, Lymph, Radiology, Prospective cohort study, business

الوصف: Background: Breast cancer-related lymphedema of the upper extremity is a fearful annoying complication for axillary clearance in breast cancer survivors. Preservation of upper extremity lymph nodes could help in reduction of incidence of the patients complaining of post axillary clearance lymph-edema. Aim of Study: To evaluate the role of focused ultrasound on lateral group of axillary lymph nodes in evaluation of clinically palpable axillary lymph nodes thus sparing the non-suspicious lateral group by ultrasound and prevention of upcoming lymphedema. Patients and Methods: This prospective study was con-ducted on fifty patients with T1 and T2 breast cancer and pathological axillary lymph nodes by ultrasound. They were scheduled for either conservative breast surgery or modified radical mastectomy with axillary clearance. Lateral group of axillary lymph nodes were divided preoperatively by focused axillary ultrasound into 2 groups. Group A included non-suspicious lateral axillary lymph nodes while group B included suspicious lateral axillary lymph nodes. During axillary dissection, the lateral group of axillary nodes (lying lateral to the thoracodorsal pedicle) was sent separately for histopatho-logical assessment (paraffin). Results: In Group A, 40 patients had a free lateral group by focused axillary US. By postoperative histopathology examination, 37 of them were confirmed free and only 3 cases proved to have metastatic deposits. While in group B, 10 patients had suspicious lateral group by focused axillary US. By postoperative histopathological examination, 8 of them had metastatic deposits and only 2 cases were free of the lateral group. So we concluded that the sensitivity of focused ultrasound of lateral group of axillary lymph nodes was 72.73%, the specificity was 94.87% and the accuracy was 90% in patients who had already suspicious lymph nodes by axillary US. Conclusion: Focused ultrasound of lateral group of axillary lymph nodes has moderate sensitivity, high specificity and accuracy. Sparing of free lateral group in patients with breast cancer could be a helpful surgical procedure aiming to reduc-tion of the rate of upper extremity lymphedema.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::84a390ad2068d1e2b0acb865743a6f4cTest
https://doi.org/10.21608/mjcu.2021.197228Test

المؤلفون: Mohamed H Zaid, Moheb S Eskandaros, Ahmed Darwish, Alaa Abbass

المصدر: Obesity Surgery. 31:4673-4681

مصطلحات موضوعية: medicine.medical_specialty, Nutrition and Dietetics, business.industry, Endocrinology, Diabetes and Metabolism, Reflux, Anastomosis, medicine.disease, Obesity, Roux-en-Y anastomosis, humanities, digestive system diseases, Surgery, law.invention, Randomized controlled trial, Weight loss, law, medicine, GERD, medicine.symptom, business, Esophagitis

الوصف: Patients with obesity having GERD usually undergo Roux-en-Y gastric bypass (RYGB) as the procedure of choice. However, the emergence of one anastomosis gastric bypass (OAGB) as a less time-consuming operation with fewer complications offers a potential option for these patients. This randomized controlled trial included 80 patients (out of 457 screened) with mild-to-moderate GERD that were equally divided into two groups for OAGB and RYGB. GERD was diagnosed by 20-item questionnaire, upper endoscopy, 24-h pH monitoring, and manometry. Follow-up at 6 and 12 months was done. No significant differences were found between the two groups regarding demographic data, comorbidities, and weight loss. OAGB had less operative time and fewer complications. Both procedures had comparable favorable effects in reducing the GERD symptoms evidenced by upper endoscopy, 24-h pH monitoring, and manometry. OAGB is a promising bariatric procedure in weight loss for patients with obesity having mild-to-moderate GERD (up to grade B esophagitis by Los Angeles score). Furthermore, wide-scale studies and on more severe degrees of GERD are required to fully understand its benefits in GERD patients with obesity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::3802b1e739268183f2c3b9dbccd42ef5Test
https://doi.org/10.1007/s11695-021-05667-5Test

المؤلفون: Moheb S. Eskandaros, M.D., Mohamed H. Zaid, M.D, A. Darwish, M.D.v, Ahmed

المصدر: The Medical Journal of Cairo University. 89:1111-1117

مصطلحات موضوعية: medicine.medical_specialty, Resuscitation, business.industry, Glasgow Coma Scale, Peritonitis, Mean age, medicine.disease, Surgery, Blunt, Associated injury, Haemodynamic instability, medicine, Prospective cohort study, business

الوصف: Background: In recent years, there is a success of Non-operative management of blunt liver trauma. Aim of Study: The aim of our study is to discuss our experience in Operative and Non-Operative management of Blunt liver Trauma. Patients and Methods: A prospective cohort enrolled twenty-seven patients with blunt liver trauma admitted to our hepato-biliary unit were managed with non-operative conservative management, Angioembolization or operative intervention. Results: The patients mean age was 31.1±7.51 years. Two (7.4%) patients were diabetic, three (11.1%) were hypertensive. Twenty-two (81.5%) patients Glasgow coma score >10. Twenty (74%) patients were managed Non-operatively. Nineteen (95%) patients had successful Non-operative management but one patient (5%)patient needed delayed operation. Seven (25.9%) patients were managed operatively. The most com-monly associated injury was chest (59.2%), head (33.3%), spleen (11.1 %) and kidney (3.7%). Liver related complications included biloma & pseudo-aneurysm. The overall mortality was (7.4%). Conclusion: NOM of blunt liver injuries in haemodynam-ically stable patients is safe and feasible. Haemodynamic instability, generalized peritonitis during resuscitation or associated intra-abdominal organ injuries requires early surgical intervention. Liver related complications contribute to failure of NOM. Associated extra-abdominal and intra-abdominal injuries do not interfere with non-operative management.v

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::c352673930d743a5a2e9d6d045a2c402Test
https://doi.org/10.21608/mjcu.2021.184998Test

المؤلفون: Moheb S, Eskandaros, Alaa, Abbass, Mohamed H, Zaid, Ahmed A, Darwish

المصدر: Obesity surgery. 32(5)

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=pmid________::8e1b7bccb136ec0c938491cc02f39f2fTest
https://pubmed.ncbi.nlm.nih.gov/35041123Test