المؤلفون: Abigail C. Dieter, Aliyah B. K. True, Emily A. Gilbertson, Grace Snyder, Adam Lacy-Hulbert, Nikki Traylor-Knowles, William E. Browne, Lauren E. Vandepas

المصدر: Frontiers in Marine Science, Vol 10 (2023)

مصطلحات موضوعية: cellular biology, vital dyes, ctenophora, FACS, cell sorting, mnemiopsis, Science, General. Including nature conservation, geographical distribution, QH1-199.5

الوصف: Cell suspension fluidics, such as flow cytometry (FCS) and fluorescence-activated cell sorting (FACS), facilitates the identification and precise separation of individual cells based on phenotype. Since its introduction, flow cytometry has been used to analyze cell types and cellular processes in diverse non-vertebrate taxa, including cnidarians, molluscs, and arthropods. Ctenophores, which diverged very early from the metazoan stem lineage, have emerged as an informative clade for the study of metazoan cell type evolution. We present standardized methodologies for flow cytometry-mediated identification and analyses of cells from the model ctenophore Mnemiopsis leidyi that can also be applied to isolate targeted cell populations. Here we focus on the identification and isolation of ctenophore phagocytes. Implementing flow cytometry methods in ctenophores allows for fine scale analyses of fundamental cellular processes conserved broadly across animals, as well as potentially revealing novel cellular phenotypes and behaviors restricted to the ctenophore lineage.

وصف الملف: electronic resource

العلاقة: https://www.frontiersin.org/articles/10.3389/fmars.2023.1276041/fullTest; https://doaj.org/toc/2296-7745Test

الوصول الحر: https://doaj.org/article/0c509da849724eb797db7f32b0b8c129Test

المؤلفون: Georges N. Nakhoul, Ali Mehdi, Jonathan J. Taliercio, Susana Arrigain, Jesse D. Schold, Abby Spencer, Jessica Greenfield, Amit Diwakar, Grace Snyder, John O’Toole, Joseph V. Nally, John R. Sedor, Patricia F. Kao, S. Beth Bierer

المصدر: BMC Nephrology, Vol 22, Iss 1, Pp 1-12 (2021)

مصطلحات موضوعية: Nephrology fellowship, Specialty training, Education, Diseases of the genitourinary system. Urology, RC870-923

الوصف: Abstract Background Interest in nephrology has been declining among internal medicine residents but the reasons behind this observation are not well characterized. Our objective was to evaluate factors influencing residents’ choice of subspecialty. Methods This is a mixed-method QUAL-QUAN design study that used the results of our previously published qualitative analysis on residents’ perception of nephrology to create and pilot a questionnaire of 60 questions. The final questionnaire was distributed to 26 programs across the United States and a total of 1992 residents. We calculated response rates and tabulated participant characteristics and percentage of participant responses. We categorized choice of fellowship into 2 medical categories (Highly Sought After vs. Less Sought After) and fitted a logistic regression model of choosing a highly vs. less sought after fellowship. Results Four hundred fifteen out of 1992 (21%) US residents responded to the survey. Of the 268 residents planning to pursue fellowship training, 67 (25%) selected a less sought after fellowship. Female sex was associated with significantly higher odds of selecting a less sought after fellowship (OR = 2.64, 95% CI: 1.47, 4.74). Major factors deterring residents from pursuing nephrology were perception of inadequate financial compensation, broad scope of clinical practice and complexity of patient population. We observed a decline in exposure to nephrology during the clinical years of medical school with only 35.4% of respondents rotating in nephrology versus 76.8% in residency. The quality of nephrology education was rated less positively during clinical medical school years (median of 50 on a 0–100 point scale) compared to the pre-clinical years (median 60) and residency (median 75). Conclusion Our study attempts to explain the declining interest in nephrology. Results suggest potential targets for improvement: diversified trainee exposure, sub-specialization of nephrology, and increased involvement of nephrologists in the education of trainees.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2369Test

الوصول الحر: https://doaj.org/article/997546ec0fcd49049548e36a6b371744Test

المؤلفون: Georges N. Nakhoul (10845856), Ali Mehdi (9018785), Jonathan J. Taliercio (10845859), Susana Arrigain (10845862), Jesse D. Schold (10845865), Abby Spencer (10845868), Jessica Greenfield (10293060), Amit Diwakar (10845871), Grace Snyder (10845874), John O’Toole (10845877), Joseph V. Nally (10845880), John R. Sedor (7572935), Patricia F. Kao (10845883), S. Beth Bierer (10845886)

مصطلحات موضوعية: Medicine, Cancer, Science Policy, Nephrology fellowship, Specialty training, Education

الوصف: Additional file 1: Table S1. Characteristics of Programs participating in the survey.

العلاقة: https://figshare.com/articles/journal_contribution/Additional_file_1_of_What_do_you_think_about_nephrology_A_national_survey_of_internal_medicine_residents/14643127Test

الإتاحة: https://doi.org/10.6084/m9.figshare.14643127.v1Test

المؤلفون: Samantha M. Baxter, Jennifer E. Posey, Nicole J. Lake, Nara Sobreira, Jessica X. Chong, Steven Buyske, Elizabeth E. Blue, Lisa H. Chadwick, Zeynep H. Coban-Akdemir, Kimberly F. Doheny, Colleen P. Davis, Monkol Lek, Christopher Wellington, Shalini N. Jhangiani, Mark Gerstein, Richard A. Gibbs, Richard P. Lifton, Daniel G. MacArthur, Tara C. Matise, James R. Lupski, David Valle, Michael J. Bamshad, Ada Hamosh, Shrikant Mane, Deborah A. Nickerson, Heidi L. Rehm, Anne O’Donnell-Luria, Marcia Adams, François Aguet, Gulsen Akay, Peter Anderson, Corina Antonescu, Harindra M. Arachchi, Mehmed M. Atik, Christina A. Austin-Tse, Larry Babb, Tamara J. Bacus, Vahid Bahrambeigi, Suganthi Balasubramanian, Yavuz Bayram, Arthur L. Beaudet, Christine R. Beck, John W. Belmont, Jennifer E. Below, Kaya Bilguvar, Corinne D. Boehm, Eric Boerwinkle, Philip M. Boone, Sara J. Bowne, Harrison Brand, Kati J. Buckingham, Alicia B. Byrne, Daniel Calame, Ian M. Campbell, Xiaolong Cao, Claudia Carvalho, Varuna Chander, Jaime Chang, Katherine R. Chao, Ivan K. Chinn, Declan Clarke, Ryan L. Collins, Beryl Cummings, Zain Dardas, Moez Dawood, Kayla Delano, Stephanie P. DiTroia, Harshavardhan Doddapaneni, Haowei Du, Renqian Du, Ruizhi Duan, Mohammad Eldomery, Christine M. Eng, Eleina England, Emily Evangelista, Selin Everett, Jawid Fatih, Adam Felsenfeld, Laurent C. Francioli, Christian D. Frazar, Jack Fu, Emmanuel Gamarra, Tomasz Gambin, Weiniu Gan, Mira Gandhi, Vijay S. Ganesh, Kiran V. Garimella, Laura D. Gauthier, Danielle Giroux, Claudia Gonzaga-Jauregui, Julia K. Goodrich, William W. Gordon, Sean Griffith, Christopher M. Grochowski, Shen Gu, Sanna Gudmundsson, Stacey J. Hall, Adam Hansen, Tamar Harel, Arif O. Harmanci, Isabella Herman, Kurt Hetrick, Hadia Hijazi, Martha Horike-Pyne, Elvin Hsu, Jianhong Hu, Yongqing Huang, Jameson R. Hurless, Steve Jahl, Gail P. Jarvik, Yunyun Jiang, Eric Johanson, Angad Jolly, Ender Karaca, Michael Khayat, James Knight, J. Thomas Kolar, Sushant Kumar, Seema Lalani, Kristen M. Laricchia, Kathryn E. Larkin, Suzanne M. Leal, Gabrielle Lemire, Richard A. Lewis, He Li, Hua Ling, Rachel B. Lipson, Pengfei Liu, Alysia Kern Lovgren, Francesc López-Giráldez, Melissa P. MacMillan, Brian E. Mangilog, Stacy Mano, Dana Marafi, Beth Marosy, Jamie L. Marshall, Renan Martin, Colby T. Marvin, Michelle Mawhinney, Sean McGee, Daniel J. McGoldrick, Michelle Mehaffey, Betselote Mekonnen, Xiaolu Meng, Tadahiro Mitani, Christina Y. Miyake, David Mohr, Shaine Morris, Thomas E. Mullen, David R. Murdock, Mullai Murugan, Donna M. Muzny, Ben Myers, Juanita Neira, Kevin K. Nguyen, Patrick M. Nielsen, Natalie Nudelman, Emily O’Heir, Melanie C. O’Leary, Chrissie Ongaco, Jordan Orange, Ikeoluwa A. Osei-Owusu, Ingrid S. Paine, Lynn S. Pais, Justin Paschall, Karynne Patterson, Davut Pehlivan, Benjamin Pelle, Samantha Penney, Jorge Perez de Acha Chavez, Emma Pierce-Hoffman, Cecilia M. Poli, Jaya Punetha, Aparna Radhakrishnan, Matthew A. Richardson, Eliete Rodrigues, Gwendolin T. Roote, Jill A. Rosenfeld, Erica L. Ryke, Aniko Sabo, Alice Sanchez, Isabelle Schrauwen, Daryl A. Scott, Fritz Sedlazeck, Jillian Serrano, Chad A. Shaw, Tameka Shelford, Kathryn M. Shively, Moriel Singer-Berk, Joshua D. Smith, Hana Snow, Grace Snyder, Matthew Solomonson, Rachel G. Son, Xiaofei Song, Pawel Stankiewicz, Taylorlyn Stephan, V. Reid Sutton, Abigail Sveden, Diana Cornejo Sánchez, Monica Tackett, Michael Talkowski, Machiko S. Threlkeld, Grace Tiao, Miriam S. Udler, Laura Vail, Zaheer Valivullah, Elise Valkanas, Grace E. VanNoy, Qingbo S. Wang, Gao Wang, Lu Wang, Michael F. Wangler, Nicholas A. Watts, Ben Weisburd, Jeffrey M. Weiss, Marsha M. Wheeler, Janson J. White, Clara E. Williamson, Michael W. Wilson, Wojciech Wiszniewski, Marjorie A. Withers, Dane Witmer, Lauren Witzgall, Elizabeth Wohler, Monica H. Wojcik, Isaac Wong, Jordan C. Wood, Nan Wu, Jinchuan Xing, Yaping Yang, Qian Yi, Bo Yuan, Jordan E. Zeiger, Chaofan Zhang, Peng Zhang, Yan Zhang, Xiaohong Zhang, Yeting Zhang, Shifa Zhang, Huda Zoghbi, Igna van den Veyver

المصدر: Genet Med

مصطلحات موضوعية: Phenotype, Exome Sequencing, Humans, Exome, Genomics, Article, Genetic Association Studies, Genetics (clinical)

الوصف: PURPOSE: Mendelian disease genomic research has undergone a massive transformation over the past decade. With increasing availability of exome and genome sequencing, the role of Mendelian research has expanded beyond data collection, sequencing, and analysis to worldwide data sharing and collaboration. METHODS: Over the past 10 years, the National Institutes of Health–supported Centers for Mendelian Genomics (CMGs) have played a major role in this research and clinical evolution. RESULTS: We highlight the cumulative gene discoveries facilitated by the program, biomedical research leveraged by the approach, and the larger impact on the research community. Beyond generating a list of gene-phenotype relationships and participating in widespread data sharing, the CMGs have created resources, tools, and training for the larger community to foster understanding of genes and genome variation. The CMGs have participated in a wide range of data sharing activities, including deposition of all eligible CMG data into the Analysis, Visualization, and Informatics Lab-space (AnVIL), sharing candidate genes through the Matchmaker Exchange and the CMG website, and sharing variants in Genotypes to Mendelian Phenotypes (Geno2MP) and VariantMatcher. CONCLUSION: The work is far from complete; strengthening communication between research and clinical realms, continued development and sharing of knowledge and tools, and improving access to richly characterized data sets are all required to diagnose the remaining molecularly undiagnosed patients.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b797c7738329d28c572a5aa99b98ff15Test
https://doi.org/10.1016/j.gim.2021.12.005Test

المؤلفون: Abigail C. Dieter, Aliyah B. K. True, Emily A. Gilbertson, Grace Snyder, Adam Lacy-Hulbert, Nikki Traylor-Knowles, William E. Browne, Lauren E. Vandepas

مصطلحات موضوعية: Oceanography, Marine Biology, Marine Geoscience, Biological Oceanography, Chemical Oceanography, Physical Oceanography, Marine Engineering, cellular biology, vital dyes, ctenophora, FACS, cell sorting, mnemiopsis

الوصف: Cell suspension fluidics, such as flow cytometry (FCS) and fluorescence-activated cell sorting (FACS), facilitates the identification and precise separation of individual cells based on phenotype. Since its introduction, flow cytometry has been used to analyze cell types and cellular processes in diverse non-vertebrate taxa, including cnidarians, molluscs, and arthropods. Ctenophores, which diverged very early from the metazoan stem lineage, have emerged as an informative clade for the study of metazoan cell type evolution. We present standardized methodologies for flow cytometry-mediated identification and analyses of cells from the model ctenophore Mnemiopsis leidyi that can also be applied to isolate targeted cell populations. Here we focus on the identification and isolation of ctenophore phagocytes. Implementing flow cytometry methods in ctenophores allows for fine scale analyses of fundamental cellular processes conserved broadly across animals, as well as potentially revealing novel cellular phenotypes and behaviors restricted to the ctenophore lineage.

العلاقة: https://figshare.com/articles/figure/Image_3_Flow_cytometry_methods_for_targeted_isolation_of_ctenophore_cells_tif/24355444Test

الإتاحة: https://doi.org/10.3389/fmars.2023.1276041.s003Test
https://figshare.com/articles/figure/Image_3_Flow_cytometry_methods_for_targeted_isolation_of_ctenophore_cells_tif/24355444Test

المؤلفون: Shani Talice, Shany Barkan, Grace Snyder, Aner Ottolenghi, Shir Eliachar, Ronit Ben-Romano, Shelly Oisher, Ton Sharoni, Magda Lewandowska, Eliya Sultan, Oron Goldstein, Reuven Aharoni, Uzi Hadad, Claytus Davis, Yehu Moran, Orly Gershoni-Yahalom, Nikki Traylor-Knowles, Benyamin Rosental

الوصف: Stem cells are the base for cell therapy due to their ability to self-renew, differentiate into other cell types, and live throughout the life of an organism. The initial cell therapy was bone marrow transplantation; bone marrow that contains haematopoietic stem cells is transferred from a healthy donor into a sick recipient to transfer the healthy genotype1,2. Stem cell therapy approach may be possible in corals, where there is genotypic variation in heat tolerance that influences survival during increased water temperatures3–5. However, we first need the ability to reliably isolate and transplant stem cells in Hexacorallia, which includes stony corals and sea anemones6. In this work, we used the sea anemone Nematostella vectensis as our model for candidate stem cell transplantation, as it is the only hexacorallian species that has fluorescent-tagged transgenic strains7. We established cell transplantation to show that there are cell populations exhibiting the functional characteristics of stem cells. We showed that a subpopulation of cells from N. vectensis can be transplanted from donor to recipient, are long-lived and self-renewing, can proliferate and differentiate, can integrate into the recipient, and rescue recipient animals treated with lethal doses of a chemotherapeutic agent. Lastly, we showed that this subpopulation can be enriched by sorting, using species non-specific cell markers and that similar subpopulations of cells can be isolated from other hexacorallians, including stony corals. This lays a foundation for the possibility of stem cell-based therapy in species of Hexacorallia.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::d8ea80f0508edbe8facecd88f1b20425Test
https://doi.org/10.21203/rs.3.rs-2137324/v1Test

المؤلفون: Abby L. Spencer, Amit Diwakar, Joseph V. Nally, S. Beth Bierer, Jessica Greenfield, Georges N. Nakhoul, Patricia Kao, Jonathan J. Taliercio, Ali Mehdi, Susana Arrigain, Grace Snyder, John F. O’Toole, John R. Sedor, Jesse D. Schold

المصدر: BMC Nephrology, Vol 22, Iss 1, Pp 1-12 (2021)
BMC Nephrology

مصطلحات موضوعية: Adult, Male, Nephrology, medicine.medical_specialty, Attitude of Health Personnel, education, Logistic regression, Subspecialty, Education, Odds, Sex Factors, Qualitative analysis, Surveys and Questionnaires, Internal medicine, Internal Medicine, Humans, Medicine, Financial compensation, Career Choice, business.industry, Research, Nephrology fellowship, Mentors, Work-Life Balance, Specialty training, Clinical Clerkship, Internship and Residency, Relative Value Scales, United States, Diseases of the genitourinary system. Urology, Clinical Practice, Scale (social sciences), Female, RC870-923, business

الوصف: BackgroundInterest in nephrology has been declining among internal medicine residents but the reasons behind this observation are not well characterized. Our objective was to evaluate factors influencing residents’ choice of subspecialty.MethodsThis is a mixed-method QUAL-QUAN design study that used the results of our previously published qualitative analysis on residents’ perception of nephrology to create and pilot a questionnaire of 60 questions. The final questionnaire was distributed to 26 programs across the United States and a total of 1992 residents. We calculated response rates and tabulated participant characteristics and percentage of participant responses. We categorized choice of fellowship into 2 medical categories (Highly Sought After vs. Less Sought After) and fitted a logistic regression model of choosing a highly vs. less sought after fellowship.ResultsFour hundred fifteen out of 1992 (21%) US residents responded to the survey. Of the 268 residents planning to pursue fellowship training, 67 (25%) selected a less sought after fellowship. Female sex was associated with significantly higher odds of selecting a less sought after fellowship (OR = 2.64, 95% CI: 1.47, 4.74). Major factors deterring residents from pursuing nephrology were perception of inadequate financial compensation, broad scope of clinical practice and complexity of patient population. We observed a decline in exposure to nephrology during the clinical years of medical school with only 35.4% of respondents rotating in nephrology versus 76.8% in residency. The quality of nephrology education was rated less positively during clinical medical school years (median of 50 on a 0–100 point scale) compared to the pre-clinical years (median 60) and residency (median 75).ConclusionOur study attempts to explain the declining interest in nephrology. Results suggest potential targets for improvement: diversified trainee exposure, sub-specialization of nephrology, and increased involvement of nephrologists in the education of trainees.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1af99b2e5d0d9de6fbeb784877a87e1bTest
https://doi.org/10.21203/rs.3.rs-153207/v1Test

المؤلفون: Grace Snyder, Daniel A. Shoskes

المصدر: Translational Andrology and Urology

مصطلحات موضوعية: Renal failure, Pediatrics, medicine.medical_specialty, Urology, medicine.medical_treatment, Population, 030232 urology & nephrology, 030209 endocrinology & metabolism, Review Article, Disease, End stage renal disease, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Testosterone replacement, education, Dialysis, education.field_of_study, business.industry, Testosterone (patch), Endocrinology, Reproductive Medicine, testosterone, Etiology, dialysis, Transplant patient, business

الوصف: Hypogonadism is a common problem in the end-stage renal disease (ESRD) and renal transplant population. It has widespread systemic effects and has been linked with mortality in dialysis patients and at the time of renal transplant. The etiology is likely multifactorial and most patients are afflicted by various comorbidities that can contribute to hypogonadism. Clinical manifestations are mostly nonspecific. We review the approach to the diagnosis of hypogonadism, focusing on both laboratory values and clinical signs and symptoms. We review treatment with testosterone replacement in this population and highlight various studies that tend to have small sample sizes. Though these studies provide insight into testosterone replacement, the need for larger studies is emphasized to better understand the effects and safety of therapy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11fa78ce2fcc4ba0355d538fe09753b2Test
https://doi.org/10.21037/tau.2016.08.01Test

المؤلفون: Didier A. Mandelbrot, Grace Snyder, Emilio D. Poggio, Jianbo Li, Neetika Garg

المصدر: Transplantation. 104(3)

مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Creatinine Excretion Rate, Adolescent, Urology, Renal function, Contrast Media, Kidney Function Tests, Living donor, Donor Selection, Iodine Radioisotopes, Young Adult, Sex Factors, Sex factors, Living Donors, Medicine, Humans, Aged, Retrospective Studies, Transplantation, Kidney, business.industry, Body Weight, Middle Aged, Iothalamic Acid, Renal Elimination, medicine.anatomical_structure, Creatinine, Female, Creatinine urine, Creatinine blood, business, Creatinine metabolism, Glomerular Filtration Rate

الوصف: BACKGROUND Glomerular filtration rate (GFR) assessment is a key aspect in the evaluation of living kidney donor candidates; however, data on performance of commonly used methods are limited. METHODS We examined 769 living kidney donor candidates with 24-hour urine collections assessed as accurate by comparing measured creatinine excretion rate (CER) to CER estimated using a 4-variable equation previously developed and validated using robust methodology. RESULTS Of all collections, 42.6% would have been deemed inaccurate, mostly under-collections, using the conventional weight- and gender-based CER estimation. Creatinine clearance (CrCl) overestimated I-iothalamate GFR (iGFR), estimated GFR (eGFR), underestimated iGFR, and their average [Avg (CrCl and eGFR)] essentially eliminated the GFR bias (median bias = +2.2, -5.4, and -1.0 mL/min/1.73 m, respectively; P < 0.001). This held true for all subgroups except blacks, where all 3 measures overestimated iGFR. Avg (CrCl and eGFR) also offered modestly improved accuracy compared with CrCl alone, as measured by the proportion of values falling within 10% (50.7% versus 45.3%; P = 0.009) and 30% of iGFR (94.5% versus 89.3%; P < 0.001). CONCLUSIONS When measured GFR is unavailable, the Avg (CrCl and eGFR) provides a better estimate of kidney function in kidney donor candidates than either measure alone, although in blacks the estimates are neither better nor worse.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bdee6df327147bb3bf2a7f06a1184daeTest
https://pubmed.ncbi.nlm.nih.gov/31205262Test

المؤلفون: Jennifer Locraft Cuddapah, James Gifford, Mary Grace Snyder

المصدر: Phi Delta Kappan. 94:50-54

مصطلحات موضوعية: Pedagogy, Psychology, Literal and figurative language, Education

الوصف: A close look at one professional's career change into teaching illustrates unique challenges and qualities, showing in stark relief what makes the induction smoother and the experience more successful.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::aacdb631c1443e36dabcd24ac9744dbcTest
https://doi.org/10.1177/003172171309400613Test