المؤلفون: Kelly, Susyn J, Genevskiy, Vladislav, Björklund, Sebastian, Gonzalez-Martinez, Juan F, Poeschke, Lara, Schröder, Maik, Nilius, Georg, Tatkov, Stanislav, Kocherbitov, Vitaly

المصدر: Biomacromolecules. 25(3):1578-1591

الوصف: Muco-obstructive diseases change airway mucus properties, impairing mucociliary transport and increasing the likelihood of infections. To investigate the sorption properties and nanostructures of mucus in health and disease, we investigated mucus samples from patients and cell cultures (cc) from healthy, chronic obstructive pulmonary disease (COPD), and cystic fibrosis (CF) airways. Atomic force microscopy (AFM) revealed mucin monomers with typical barbell structures, where the globule to spacer volume ratio was the highest for CF mucin. Accordingly, synchrotron small-angle X-ray scattering (SAXS) revealed more pronounced scattering from CF mucin globules and suggested shorter carbohydrate side chains in CF mucin and longer side chains in COPD mucin. Quartz crystal microbalance with dissipation (QCM-D) analysis presented water sorption isotherms of the three types of human airway mucus, where, at high relative humidity, COPD mucus had the highest water content compared to cc-CF and healthy airway mucus (HAM). The higher hydration of the COPD mucus is consistent with the observation of longer side chains of the COPD mucins. At low humidity, no dehydration-induced glass transition was observed in healthy and diseased mucus, suggesting mucus remained in a rubbery state. However, in dialyzed cc-HAM, a sorption-desorption hysteresis (typically observed in the glassy state) appeared, suggesting that small molecules present in mucus suppress the glass transition.

وصف الملف: electronic

الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-66269Test
https://doi.org/10.1021/acs.biomac.3c01170Test
https://mau.diva-portal.org/smash/get/diva2:1843158/FULLTEXT01.pdfTest

المؤلفون: de la Cerda-vargas, MF, Pantalone, MR, Naucler, CS, Medrano-Guzman, R, Renaud, KJ, Rueda, BN, Reynoso-Sanchez, MD, Lopez-Quintana, B, Rodriguez-Florido, MA, Feria-Romero, IA, Trejo-Rosales, RR, Arreola-Rosales, RL, Candelas-Rangel, JA, Navarro-Dominguez, P, Meza-Mata, E, Munoz-Hernandez, MA, Segura-Lopez, FK, Gonzalez-Martinez, MD, Delgado-Aguirre, HA, Sandoval-Bonilla, BA

المصدر: Heliyon. 10(7):e28555

مصطلحات موضوعية: Medicin och hälsovetenskap

الوصول الحر: http://kipublications.ki.se/Default.aspx?queryparsed=id:156559426Test

المؤلفون: Tafadzwa Madanhire, Grace McHugh, Victoria Simms, Lucky Ngwira, Carmen Gonzalez-Martinez, Robina Semphere, Brewster Moyo, Claire Calderwood, Mark Nicol, Tsitsi Bandason, Jon O. Odland, Andrea M. Rehman, Rashida A. Ferrand

المصدر: BMC Pulmonary Medicine, Vol 24, Iss 1, Pp 1-9 (2024)

مصطلحات موضوعية: Chronic lung disease, HIV, FEV1, Africa, Azithromycin, Children, Diseases of the respiratory system, RC705-779

الوصف: Abstract Background Chronic lung disease (CLD) is common among children with HIV (CWH) including in those taking antiretroviral therapy (ART). Azithromycin has both antimicrobial and anti-inflammatory effects and has been effective in improving lung function in a variety of lung diseases. We investigated lung function trajectories among CWH with CLD on ART enrolled in a randomized controlled trial of adjuvant azithromycin. We also investigated factors that modified the effect of azithromycin on lung function. Methods The study used data from a double-blinded placebo-controlled trial conducted in Malawi and Zimbabwe of 48 weeks on azithromycin (BREATHE: ClinicalTrials.gov NCT02426112) among CWH aged 6 to 19 years taking ART for at least six months who had a forced expiratory volume in one second (FEV1) z-score

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/1471-2466Test

الوصول الحر: https://doaj.org/article/6f858e21bc1b4a75a442e815a61f6f78Test

المؤلفون: Alexander Chan, Rebecca M. Haley, Mohd Altaf Najar, David Gonzalez-Martinez, Lukasz J. Bugaj, George M. Burslem, Michael J. Mitchell, Andrew Tsourkas

المصدر: Nature Communications, Vol 15, Iss 1, Pp 1-21 (2024)

مصطلحات موضوعية: Science

الوصف: Abstract Recently, targeted degradation has emerged as a powerful therapeutic modality. Relying on “event-driven” pharmacology, proteolysis targeting chimeras (PROTACs) can degrade targets and are superior to conventional inhibitors against undruggable proteins. Unfortunately, PROTAC discovery is limited by warhead scarcity and laborious optimization campaigns. To address these shortcomings, analogous protein-based heterobifunctional degraders, known as bioPROTACs, have been developed. Compared to small-molecule PROTACs, bioPROTACs have higher success rates and are subject to fewer design constraints. However, the membrane impermeability of proteins severely restricts bioPROTAC deployment as a generalized therapeutic modality. Here, we present an engineered bioPROTAC template able to complex with cationic and ionizable lipids via electrostatic interactions for cytosolic delivery. When delivered by biocompatible lipid nanoparticles, these modified bioPROTACs can rapidly degrade intracellular proteins, exhibiting near-complete elimination (up to 95% clearance) of targets within hours of treatment. Our bioPROTAC format can degrade proteins localized to various subcellular compartments including the mitochondria, nucleus, cytosol, and membrane. Moreover, substrate specificity can be easily reprogrammed, allowing modular design and targeting of clinically-relevant proteins such as Ras, Jnk, and Erk. In summary, this work introduces an inexpensive, flexible, and scalable platform for efficient intracellular degradation of proteins that may elude chemical inhibition.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2041-1723Test

الوصول الحر: https://doaj.org/article/c8e03480a79f42f1b05ddadc6ec0b9d3Test

المؤلفون: Angel Antonio Gonzalez Martinez, Irenilza de Alencar Nääs, Thayla Morandi Ridolfi de Carvalho-Curi, Jair Minoro Abe

المصدر: AgriEngineering, Vol 6, Iss 2, Pp 1252-1265 (2024)

مصطلحات موضوعية: non-classic logic, broiler production, exhaust fans, evaporative cooling, Agriculture (General), S1-972, Engineering (General). Civil engineering (General), TA1-2040

الوصف: Broilers are particularly sensitive to heat stress, which can impair growth, and lower conversion efficiency and survival rates. Under a climate change scenario, maintaining optimal thermal conditions within broiler houses becomes more complex and energy-intensive. Climate change can worsen air quality issues inside broiler houses by increasing the concentration of harmful gases, and proper mechanical ventilation systems are essential for diluting and removing these gases. The present study aimed to develop and validate a model for the ideal broiler housing strategy by applying the Paraconsistent Annotated Evidential Logic Eτ. A database from four broiler houses in a commercial farm, rearing 157,700 birds from the 1st to the 42nd day of growth, was used in the research. All environmental data were recorded weekly inside the houses, and on day 42, flock mortality, overall feed-to-gain ratio, and body weight were calculated and registered. The Cohen’s Kappa statistics for each environmental parameter classification compared to the paraconsistent classification. Results indicated that temperature shows good agreement, relative humidity shows slight agreement, air velocity presents a good agreement, CO2 concentration has a slight agreement, and NH3 concentration is classified by slight agreement. The environmental and productivity variables as a function of the broiler age using the extreme True paraconsistent state indicate the model validation. The paraconsistent analysis presented the ideal scenario for broilers’ growth, maintaining the environmental variables level within a particular threshold and providing greater profit to broiler farmers.

وصف الملف: electronic resource

العلاقة: https://www.mdpi.com/2624-7402/6/2/71Test; https://doaj.org/toc/2624-7402Test

الوصول الحر: https://doaj.org/article/e8dba77bd83143a092b73b5a71dad838Test

المؤلفون: González Martínez, Juan Francisco

مرشدي الرسالة: Colchero Paetz, Jaime Virgilio, Abad López, José, Universidad de Murcia. Departamento de Física

المصدر: TDR (Tesis Doctorales en Red)

مصطلحات موضوعية: Microscopios y microscopía, Ciencias

الوصف: En esta tesis se analizan y discuten los aspectos que determinan los límites de la microscopía de fuerzas (SFM). Para tal fin, se han expuesto un conjunto de modelos de diversa complejidad (oscilador armónico, modelo continuo). Además, se ha descrito con detalle el origen y significado físico de las señales electrónicas en las que convertimos el desplazamiento físico (amplitud, fase, frecuencia y fuerza normal) y el sistema electrónico encargado de su adquisición y procesado (Capítulos 1 y 2). El comportamiento dinámico del fleje no puede limitarse a un oscilador armónico ya que constituye un sistema más complejo: es necesario considerar el fleje como un conjunto de osciladores, excitados en mayor o menor medida, donde cada uno de ellos, se ve afectado por disipaciones (internas, despreciables en este contexto) y externas (de tipo hidrodinámico) que, a su vez, tienen una dependencia en función de la distancia punta-muestra. Desde el punto de vista hidrodinámico, se analiza dicha disipación a través del factor de calidad del oscilador (Capítulo 6). Dicho análisis parte de la descomposición del sistema físico (el fleje) en sus diversas partes (el fleje, propiamente dicho, la punta y el vértice de la punta) y permite la optimización de la geometría del sistema fleje-punta para conseguir la máxima sensibilidad posible. El ruido térmico es uno de los límites fundamentales en las aplicaciones desarrolladas en esta tesis y es pues, el eje central que ha permitido su confección. Las fluctuaciones térmicas afectan a la posición del fleje y limitan la determinación de la frecuencia de resonancia del sistema. Se desarrolla una relación precisa (Capítulo 3) para el ruido térmico en frecuencia en función del ancho de banda y de la amplitud de oscilación del fleje, que rectifica la relación clásica empleada en la literatura. La diferente composición química de los compuestos presentes en una superficie se refleja en la existencia de una contribución adicional al ruido térmico, y, por tanto, a los límites de las medidas en microscopía de fuerzas. Se han realizado experimentos que evidencian la presencia de este ruido y explican cómo es posible extraer información del mismo (Capítulo 5). Además, justificamos su existencia a raíz de las diferentes interacciones hidrófobica e hidrofílica presentes en la superficie. La variación de la amplitud de oscilación está relacionada con la disipación del sistema punta-muestra. Además, sólo para pequeñas amplitudes el sistema dinámico puede considerarse armónico. Es necesario desarrollar un método de calibración de la amplitud de oscilación que permita conocer con precisión cuánto varía la posición del fleje. Esto es posible mediante una calibración correcta de la señal de la amplitud de oscilación y para tal fin, se proponen dos métodos (Capítulo 4): uno de ellos está basado en el cálculo de la desviación cuadrática media de la señal de salida de la electrónica; el segundo método se basa en el análisis del espectro de la señal correspondiente y en el cálculo del factor de calidad, la frecuencia de resonancia y la intensidad de la señal. Ambos métodos son también aplicables a la calibración de la fuerza normal. En este caso la importancia radica en que es posible calibrar la sensibilidad en fuerza normal sin que la punta del fleje sufra daños. Finalmente, en los experimentos expuestos se ha hecho uso de un sistema de reposicionamiento preciso (Capítulo 7). Este sistema, permite la manipulación ex-situ de las muestras y el reposicionamiento preciso en el microscopio de fuerzas sin ayuda de un microscopio óptico o marcas de posición.

الوصف (مترجم): This thesis analyses and describes the fundamental and experimental limits of Scanning Force Microscopy. To this end, a set of models for the dynamical behaviour of the system are proposed. In addition, the origin and physical meaning of the electronic signals generated by the motion of the cantilever (amplitude, phase, frequency and normal force) and the electronics involved in its acquisition and processing have been described in detail (Chapters 1 and 2). The dynamic behaviour of the cantilever cannot be described correctly by the harmonic oscillator. It is necessary to consider the cantilever as a set of oscillators, excited in different modes, each of them being affected by dissipations (internal, negligible in this context) and external (hydrodynamic) that, in turn, depends on the tip-sample distance. From the hydrodynamic point of view, this dissipation is analysed through the quality factor of the oscillator (Chapter 6). This analysis is based on the decomposition of the system (the cantilever) in its components (the lever, the tip and the apex of the tip) and allows the optimization of the geometry of the system (cantilever) in order to obtain the maximum sensitivity possible. The thermal noise is one of the fundamental limits of Scanning Force Microscopy. Temperature fluctuations affect the position of the cantilever and limit the resolution of the frequency measurements. A precise relationship (Chapter 3) for thermal frequency noise as a function of the bandwidth and amplitude of oscillation of the cantilever has been developed. This result rectifies the classic relationship from the scientific literature. We have found that the chemical composition of a surface induces an additional contribution to noise, and therefore to the limits in SFM. To show and analyse in more detail the presence of this noise and the information it contains, various experiments have been developed (Chapter 5). Furthermore, we justify the nature of this noise from the different hydrophobic and hydrophilic interactions on the surface. The fluctuations in the amplitude of oscillation is related to the dissipation of the tip-sample system. Only for small amplitudes, the dynamic system can be considered harmonic. It is important to develop a calibration method for the oscillation amplitude. For this purpose, two methods (Chapter 4) are proposed: one based on the mean square deviation calculus of the output signal of the electronics; the second method, based on spectral analysis and the calculation of the quality factor, resonant frequency and signal strength. Both methods are also applicable to the calibration of the normal force. In this case, the importance lies in the calibration of the sensitivity of normal force without tip damage. Finally, for some of the experiments reported along this thesis, a precise repositioning system (Chapter 7) has been developed. This system allows the ex-situ sample handling and the precise repositioning of the sample in the SFM without the aid of an optical microscope or position marks.

وصف الملف: application/pdf

الوصول الحر: http://hdl.handle.net/10803/334415Test

المؤلفون: González Martínez, María Teresa

المساهمون: University/Department: Universitat Autònoma de Barcelona. Departament de Ciència Animal i dels Aliments

مرشدي الرسالة: González Martínez, Blanca Edelia, Hernández Herrero, Manuela

المصدر: TDX (Tesis Doctorals en Xarxa)

مصطلحات موضوعية: Queso chihuahua, Histamina, Tiramina, Ciències de la Salut

الوصف: La inocuidad de los alimentos constituye una de las preocupaciones de la industria de alimentos ya que afecta directamente a la salud de los consumidores. El queso chihuahua es el queso madurado de mayor consumo en México, la maduración se realiza mediante la adición de microorganismos lácticos, principalmente del género Lactococcus y en algunos casos también Streptococcus thermophilus y Lactobacillus. Se ha demostrado que en alimentos madurados se pueden desarrollar aminas biógenas por la presencia de enzimas amino descarboxilasas de los microorganismos fermentadores. El consumo de histamina y tiramina pueden ser de riesgo para los consumidores, en especial para quienes presentan inhibición de la enzima mono amino oxidasa. El objetivo de este trabajo fue evaluar el desarrollo de histamina y tiramina en 8 quesos chihuahua elaborados con leche pasteurizada durante la vida de anaquel. Se inició con el análisis de la calidad microbiológica y físico-química de los quesos requerida por la normatividad vigente, se desarrollaron cultivos en medios selectivos para identificar bacterias del queso con enzimas histidina y tirosina descarboxilasa (HDC y TDC) utilizando un caldo sintético con histidina o tirosina añadido, este estudio se realizó en 4 momentos de la vida de anaquel y para identificar las bacterias ácido láctico (BAL) con enzimas descarboxilasas en las pruebas positivas, se utilizó el sistema bioquímico API 50 CH (Biomerieux). Al mismo tiempo, se cuantificó la presencia de histamina y tiramina por cromatografía de líquidos de alta resolución (HPLC) en tres diferentes momentos, al inicio y a la mitad de la vida de anaquel, y en la fecha de caducidad del producto. Por otra parte, de los quesos se realizó una extracción directa de ADN bacteriano, donde se desarrolló una metodología que incluye un proceso de eliminación de la grasa. Se analizó la presencia de genes que codifican para las enzimas histidina y tirosina descarboxilasa por PCR (reacción de la cadena de la polimerasa) utilizando oligonucleótidos previamente reportados para hdc y diseñando nuevos oligonucleótidos para tdc. Los resultados microbiológicos mostraron que los quesos cumplen con la normatividad mexicana para estos productos, la cuenta total de bacterias fue de 3,10 a 3,80 Log UFC/g, de coliformes de 2,6 a 3,4 Log UFC/g. No se detectaron bacterias patógenas como Salmonella, Listeria monocytogenes ni Staphylococcus aureus. Los estudios físico químicos muestran variaciones importantes entre las marcas en las concentraciones de sodio, proteína y grasa en algunos casos los valores encontrados no corresponden a lo declarado en las etiquetas. La detección de microorganismos con enzima HDC al final de la vida de anaquel fue en un 37,5% de las muestras y para microorganismos con la enzima TDC en un 75%. Se identificaron Lactobacillus pentosus con enzimas HDC y TDC en 3 productos y L. rhamnosus con enzima TDC en 2 productos. Al inicio del almacenamiento ninguno de los quesos presentaba histamina y solo el 37,5% contenían tiramina en concentraciones de 34 a 122 mg/Kg de queso, mientras que al final de la vida de anaquel en el 75% de los quesos se detectó tiramina (115 a 209 mg/Kg de queso) y en el 37% se encontró histamina (47 a 205 mg/Kg de queso). En 6 de los 8 quesos analizados (75%) se detectó el gen tdc, mientras que en solo 3 (37,5%) se logró detectar el gen hdc. Al asociar los resultados de HPLC al final del almacenamiento con la presencia de los genes tdc y hdc se observa una correlación significativa de 0,001 y 0,024 respectivamente. Es indispensable disminuir la formación de aminas biógenas en los quesos ya sea por pasteurización de la leche, selección de las cepas iniciadoras y/o con buenas prácticas higiénicas.

الوصف (مترجم): Food safety is a concern in the food industry since it directly affects the consumer health. Chihuahua cheese is the mature cheese of major consumption in Mexico; the maturation is realized by adding lactic microorganisms, especially Lactococcus and in some cases Streptococcus thermophiles and Lactobacillus. It has been demonstrated that mature food can develop biogenic amines because of the presence of amino descarboxilasas enzymes of the fermenter microorganisms. The consumption of histamine and tyramine can be risky for consumers, especially for those who present inhibition of monoamine oxidase enzyme. The aim of this study was to evaluate the development of histamine and tyramine in 8 chihuahua cheeses elaborated with pasteurized milk during its shelf life. It began with the analysis of the microbiological and physical-chemical quality of the cheeses required by current regulations; selective media cultures were developed to identify bacteria in cheeses with enzymes histidine and tyrosine decarboxylase (HDC y TDC) using a synthetic broth with histidine or tyrosine added, this study was made in 4 times of the shelf life and to identify the lactic acid bacteria (BAL) with descarboxilasa enzyme in the positive tests, the biochemical system API 50 CH (Biomerieux) was used. At the same time, the presence of histamine and tyramine was quantified by high- performance liquid chromatography (HPLC) in three different moments, at the beginning and middle of shelf life and at the product expiration. On the other hand, bacterial ADN was extracted directly from cheese to develop a methodology that includes a fat removal process. It was analyzed the presence of genes that encode the histidine and tyrosine decarboxylase enzyme by PCR reaction (Polymerase Chain) using primers previously reported for hdc and designing new primers for tdc. The microbiological results showed that the cheeses fulfill the Mexican regulations for these products, the total account of bacteria was from 3,10 to 3,80 Log UFC/g, and coliformes from 2,6 to 3,4 Log UFC/g. Pathogenic bacteria such as Salmonella, Listeria monocytogenes or Staphylococcus aureus were not found. The physical-.chemical studies show important variations between the marks in the concentrations of sodium, protein and fat, in some cases the values do not correspond to the ones declared in the labels. The detection of microorganisms with enzyme HDC at the end of shelf life was 37,5% of the samples and microorganisms with the enzyme TDC was 75%. Lactobacillus pentosus were identified with enzymes HDC and TDC in 3 products and L. rhamnosus with enzyme TDC in 2 products. At the beginning of storage none of the cheeses had histamine and only 37.5% contained tyramine in concentrations from 34 to 122 mg/Kg of cheese, whereas at the end of the shelf life 75% of the cheeses had tyramine (115 to 209 mg/Kg of cheese) and 37% histamine (47-205 mg/Kg of cheese). Six of the eight cheeses analyzed (75%) had tdc gene whereas only 3 (37,5%) contained the hdc gene. The results of HPLC at the end of storage in the presence of tdc and hdc genes show a significant correlation of 0,001 and 0,024 respectively. It is indispensable to decrease the formation of biogenic amines in cheeses either by pasteurization of milk, selection of the strains initiators and/or with good hygiene practices.

وصف الملف: application/pdf

الوصول الحر: http://hdl.handle.net/10803/129087Test

المؤلفون: Matthew Pease, Jonathan Elmer, Arka N. Mallela, Jorge Gonzalez-Martinez, David O. Okonkwo, Flora Hammond, Sergiu Abramovici, James F. Castellano, Wesley T. Kerr

المصدر: Neurotrauma Reports, Vol 5, Iss 1, Pp 330-336 (2024)

مصطلحات موضوعية: anti-seizure medicine, early seizures, epilepsy, levetiracetam, seizure prophylaxis, phenytoin, seizures, Medical emergencies. Critical care. Intensive care. First aid, RC86-88.9

الوصف: Seizures are common after severe traumatic brain injury (TBI), with rates in the acute period approaching 5% with seizure prophylaxis in historical clinical trials. Post-traumatic seizures (PTS) are divided into categories: immediate PTS occur prior to resuscitation, typically in the field; early PTS occur from resuscitation to 7 days post-trauma; and late PTS occur thereafter. The relationship between immediate and early PTS, as well as their risk factors, are not well studied in modern cohorts. We performed a secondary analysis of a prospective database of severe TBI patients, defined as a post-resuscitation Glasgow Coma Scale ?8, from a single institution. For the 579 patients included, rates of immediate and early PTS were 1.6% and 3.8%, respectively. We were unable to identify any clinical correlates for immediate seizures. In contrast, early PTS were associated with age (odds ratio [OR] 1.5; 95% confidence interval [CI]: 1.1?2.0; p?0.01), hypoxia (3.3, 95% CI: 1.2?8.5; p?=?0.02), and subdural hematoma (SDH) (2.8, 95% CI: 1.0?2.8; p?=?0.04) in multivariable modeling. Patients with early PTS had higher rates of status epilepticus than those with immediate PTS (45% [n?=?10/22] vs. 0% [n?=?0/9]; p?=?0.03). This supports the notion of immediate PTS, which typically occur in the field and may not reliably be deciphered from pathological posturing responses, as an entity distinct from early PTS. Status epilepticus was highly morbid, associated with a 70% mortality rate. Our previously identified markers may help risk-stratify patients who may warrant longer monitoring with continuous electroencephalography to detect and treat early PTS and corresponding status epilepticus risk.

وصف الملف: electronic resource

العلاقة: https://doaj.org/toc/2689-288XTest

الوصول الحر: https://doaj.org/article/01cc4bc3237d4554a586b2ff2af55b4dTest

المؤلفون: González Martínez, Juan

المساهمون: University/Department: Universitat Rovira i Virgili. Departament de Pedagogia

مرشدي الرسالة: juan.gonzalezm@urv.cat, Espuny Vidal, Cinta, Gisbert Cervera, Mercè

المصدر: TDX (Tesis Doctorals en Xarxa)

مصطلحات موضوعية: Tecnologia educativa, Educació secundària, Competència digital, eduCAT1x1, one-to-one, Competència comunicativa

الوصف: La present tesi realitza un seguiment del primer any d’implementació del projecte eduCAT1x1 a la demarcació educativa de les Terres de l’Ebre, on durant el curs 2010/2011 s’ha generalitzat un model basat en la completa digitalització de les aules d’Educació Secundària Obligatòria (incorporació de pissarres digitals interactives, distribució d’un microportàtil a cada alumne, generalització dels llibres digitals, connexió de les aules a la xarxa, etc.). Aquest seguiment focalitza especialment en els aspectes relatius a la infraestructura, a la competència digital de l’alumnat i a l’aprofitament al si d’una de les assignatures; i està previst realitzar-lo amb tres Instituts d’Ensenyament Secundari públics de la demarcació. Pel que fa a la implementació, es detecten mancances pel que fa a les infraestructures, a la qualitat dels materials educatius digitals i a les possibilitats de compartició, a la productivitat de les tecnologies i a la formació del professorat; pel que fa a la competència digital de l’alumnat, es constaten mancances en relació a la seva capacitat de fer servir les TIC per a l’aprenentatge; i finalment, pel que fa a l’aprofitament a l’assignatura de Llengua Castellana, atès el baix aprenentatge de la competència comunicativa, es detecta un greu problema curricular i un escàs rendiment de la innovació aplicada. Una vegada analitzada la implementació, es formulen diferents propostes de millora adreçades a millorar la realitat d’eduCAT1x1.

الوصف (مترجم): This Ph. D. report presents the explanation of the the first eduCAT1x1 project year implementation in the educational demarcation of Terres de l’Ebre (Tarragona), during the school year 2010/2011, where it has been generalized a model based on the complete Secondary Education classrooms digitization (incorporating interactive whiteboards, microlaptops for each student, digital books, connecting classrooms to the Internet, etc.). This report focuses particularly on matters relating to infrastructure, the students digital competence and the improving within one of the subjects, and it has been developed in three public high schools from our area. Regarding the implementation issue, we have found weaknesses in terms of infrastructures, the quality of digital educational materials, the possibilities of knowledge sharing and teacher training options; regarding the students digital competence, we’ve shown clear shortcomings in relation to their ability to use ICT for their own learning; and, finally, regarding the real improving within the Spanish language subject, it is very clear the low level of communicative competence learning, so we’ve detected a serious curricular problem among others. Once exposed the implementation process, we formulate several learning improving proposals in the context of this eduCAT1x1 project.

وصف الملف: application/pdf

الوصول الحر: http://hdl.handle.net/10803/84032Test

المؤلفون: Paracini, Nicolo, Gutfreund, Philipp, Welbourn, Rebecca, Gonzalez-Martinez, Juan Francisco, Zhu, Kexin, Miao, Yansong, Yepuri, Nageshwar, Darwish, Tamim A. ., Garvey, Christopher, Waldie, Sarah, Larsson, Johan, Wolff, Max, Cardenas, Marite

المصدر: ACS Applied Materials and Interfaces. 15(3):3772-3780

مصطلحات موضوعية: nanoparticle-supported lipid bilayers, nanoSLB, membrane curvature, model membranes, neutron reflectometry, GISANS, GISAXS

الوصف: Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We combined scattering techniques with direct imaging, to gain access to sub-nanometer scale structural information on stable nanoparticle monolayers assembled on silicon crystals in a noncovalent manner using a Langmuir-Schaefer deposition. The structure of supported lipid bilayers formed on the nanoparticle arrays via vesicle fusion was investigated using a combination of grazing incidence X-ray and neutron scattering techniques complemented by fluorescence microscopy imaging. Ordered nanoparticle assemblies were shown to be suitable and stable substrates for the formation of curved and fluid lipid bilayers that retained lateral mobility, as shown by fluorescence recovery after photobleaching and quartz crystal microbalance measurements. Neutron reflectometry revealed the formation of high-coverage lipid bilayers around the spherical particles together with a flat lipid bilayer on the substrate below the nanoparticles. The presence of coexisting flat and curved supported lipid bilayers on the same substrate, combined with the sub-nanometer accuracy and isotopic sensitivity of grazing incidence neutron scattering, provides a promising novel approach to investigate curvature-dependent membrane phenomena on supported lipid bilayers.

وصف الملف: electronic

الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-512532Test
https://doi.org/10.1021/acsami.2c18956Test
https://uu.diva-portal.org/smash/get/diva2:1800587/FULLTEXT01.pdfTest